LOCUS       BBa_I10337               570 bp    DNA     linear       25-Nov-2024
DEFINITION  R0040-E0433 composite
ACCESSION   BBa_I10337
VERSION     BBa_I10337.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..428
                     /label=lacZ alpha
     misc_feature    161..161
                     /label=T
     misc_feature    167..167
                     /label=A
     misc_feature    188..188
                     /label=C
     misc_feature    158..158
                     /label=C
     misc_feature    191..191
                     /label=G
     promoter        106..115
                     /label=T3 promoter
     promoter        253..271
                     /label=T7 promoter
     misc_feature    279..428
                     /label=lacZ gene fragment
     misc_feature    81..95
                     /label=lacZ gene fragment
     misc_feature    81..433
                     /label=BBa_E0033
     stem_loop       453..496
                     /label=stem_loop
     misc_feature    442..521
                     /label=BBa_B0010
     terminator      442..521
                     /label=BBa_B0010
     stem_loop       537..556
                     /label=T7 TE
     misc_feature    563..563
                     /label=stop
     polyA_site      557..570
                     /label=polya
     misc_feature    530..570
                     /label=BBa_B0012
     terminator      530..570
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaccatga ttacgccaag cgcgcaatta accctcacta
      121 aagggaacaa aagctggagc tccaccgcgg tggcggcagc actagagcta gtggatcccc
      181 cgggctgtag aaattcgata tcaagcttat cgataccgtc gacctcgagg gggggcccgg
      241 tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac
      301 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
      361 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
      421 gcctgaataa taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact
      481 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
      541 caccttcggg tgggcctttc tgcgtttata
//