##gff-version 3
##sequence-region BBa_I10499 1 903
BBa_I10499	.	ribosome_entry_site	1	13	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_I10499	.	ribosome_entry_site	7	10	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_I10499	.	ribosome_entry_site	1	13	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_I10499	.	engineered_region	1	13	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_I10499	.	terminator	809	903	.	+	0	ID=BBa_B0014;Name=BBa_B0014
BBa_I10499	.	terminator	809	849	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0014
BBa_I10499	.	engineered_region	809	849	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I10499	.	stop_codon	842	842	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I10499	.	stem_loop	816	835	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I10499	.	polyA_site	836	849	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I10499	.	terminator	858	903	.	+	0	ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_B0014
BBa_I10499	.	stem_loop	870	892	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_I10499	.	engineered_region	858	903	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_I10499	.	CDS	20	775	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_I10499	.	point_mutation	511	511	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_I10499	.	CDS	20	769	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_I10499	.	engineered_region	20	775	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_I10499	.	engineered_tag	776	800	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_I10499	.	point_mutation	193	193	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_I10499	.	engineered_region	20	21	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
>BBa_I10499
tcacacaggaaagtactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtaga
agcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcattt
atcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgc
taatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaac
aatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttcccta
ttcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttattttt
acatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaa
tcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaa
aaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctg
ccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctag
tgtagatcactactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaa
aagccagattattaatccggcttttttattattt