BBa_I1060
1
BBa_I1060
Custom cI(2) Protein w/LacO-1 Promoter (Used for Anti-sense inhibition)
2003-01-31T12:00:00Z
2015-08-31T04:07:30Z
Modified from BioBricks <bb_part>BBa_R0011</bb_part>, and <bb_part>BBa_C0051</bb_part>.
Coding sequence for modified cI protein with LacO-1 promoter for antisense IS10 mRNA regulation.</P>
false
false
_1_
0
24
7
It's complicated
false
<P> <P> <bb_part>BBa_I1060</bb_part> Custom cI Protein with LacO-1 Promoter is based on BioBricks <bb_part>BBa_C0050</bb_part> and <bb_part>BBa_R0011</bb_part>. It is designed such that <bb_part>BBa_I1063</bb_part> can be used to interfere with the <bb_part>BBa_1060</bb_part> transcript by anti-sense mRNA binding, in the IS10 method shown below. <bb_part>BBa_I1060</bb_part> and <bb_part>BBa_I1063</bb_part> can act indepently of related biobrick parts <bb_part>BBa_1010</bb_part>, <bb_part>BBa_1011</bb_part>, <bb_part>BBa_1012</bb_part>, <bb_part>BBa_I1030</bb_part>, <bb_part>BBa_I1031</bb_part>, <bb_part>BBa_I1032</bb_part>, <bb_part>BBa_I1041</bb_part>, <bb_part>BBa_I1042</bb_part>, <bb_part>BBa_I1020</bb_part>, <bb_part>BBa_I1023</bb_part> and <bb_part>BBa_1013</bb_part>.<P> Can be used with all parts of the BBa_I system. It is inhibited by BBa_I1063. Incompatible with systems containing cI (wild type)
false
Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton
annotation1958
1
swap
range1958
1
72
72
annotation1959
1
swap
range1959
1
79
79
annotation1952
1
LacO-1 region
range1952
1
1
20
annotation1948
1
-10
range1948
1
44
49
annotation1956
1
C added
range1956
1
86
88
annotation7060
1
BBa_I1060
range7060
1
1
835
annotation1947
1
-35
range1947
1
21
26
annotation1951
1
2
range1951
1
830
835
annotation1949
1
Complemenatry to Antisense mRNA I1063
range1949
1
56
158
annotation1953
1
LacO-1
range1953
1
27
43
annotation1957
1
Binds BBa_I1063
range1957
1
89
90
annotation1943
1
rbs
range1943
1
73
78
annotation1942
1
stem_loop
range1942
1
56
85
annotation1946
1
cI lambda
range1946
1
83
835
annotation1961
1
swap
range1961
1
82
82
annotation1950
1
SsrA
range1950
1
797
835
annotation1960
1
swap
range1960
1
81
81
annotation1954
1
start
range1954
1
56
56
annotation1945
1
start
range1945
1
83
85
annotation1955
1
HH117a swap
range1955
1
58
58
BBa_I1060_sequence
1
ataaatgtgagcggataacattgacattgtgagcggataacaagatactgagcacgccaaaaatcaataatgagacaagatcatgtgcagtactaagaaaaagcctttgactcaggaacaactcgaagatgcccgccgtctcaaggccatctacgagaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z