BBa_I1060 1 BBa_I1060 Custom cI(2) Protein w/LacO-1 Promoter (Used for Anti-sense inhibition) 2003-01-31T12:00:00Z 2015-08-31T04:07:30Z Modified from BioBricks <bb_part>BBa_R0011</bb_part>, and <bb_part>BBa_C0051</bb_part>. Coding sequence for modified cI protein with LacO-1 promoter for antisense IS10 mRNA regulation.</P> false false _1_ 0 24 7 It's complicated false <P> <P> <bb_part>BBa_I1060</bb_part> Custom cI Protein with LacO-1 Promoter is based on BioBricks <bb_part>BBa_C0050</bb_part> and <bb_part>BBa_R0011</bb_part>. It is designed such that <bb_part>BBa_I1063</bb_part> can be used to interfere with the <bb_part>BBa_1060</bb_part> transcript by anti-sense mRNA binding, in the IS10 method shown below. <bb_part>BBa_I1060</bb_part> and <bb_part>BBa_I1063</bb_part> can act indepently of related biobrick parts <bb_part>BBa_1010</bb_part>, <bb_part>BBa_1011</bb_part>, <bb_part>BBa_1012</bb_part>, <bb_part>BBa_I1030</bb_part>, <bb_part>BBa_I1031</bb_part>, <bb_part>BBa_I1032</bb_part>, <bb_part>BBa_I1041</bb_part>, <bb_part>BBa_I1042</bb_part>, <bb_part>BBa_I1020</bb_part>, <bb_part>BBa_I1023</bb_part> and <bb_part>BBa_1013</bb_part>.<P> Can be used with all parts of the BBa_I system. It is inhibited by BBa_I1063. Incompatible with systems containing cI (wild type) false Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton annotation1958 1 swap range1958 1 72 72 annotation1959 1 swap range1959 1 79 79 annotation1952 1 LacO-1 region range1952 1 1 20 annotation1948 1 -10 range1948 1 44 49 annotation1956 1 C added range1956 1 86 88 annotation7060 1 BBa_I1060 range7060 1 1 835 annotation1947 1 -35 range1947 1 21 26 annotation1951 1 2 range1951 1 830 835 annotation1949 1 Complemenatry to Antisense mRNA I1063 range1949 1 56 158 annotation1953 1 LacO-1 range1953 1 27 43 annotation1957 1 Binds BBa_I1063 range1957 1 89 90 annotation1943 1 rbs range1943 1 73 78 annotation1942 1 stem_loop range1942 1 56 85 annotation1946 1 cI lambda range1946 1 83 835 annotation1961 1 swap range1961 1 82 82 annotation1950 1 SsrA range1950 1 797 835 annotation1960 1 swap range1960 1 81 81 annotation1954 1 start range1954 1 56 56 annotation1945 1 start range1945 1 83 85 annotation1955 1 HH117a swap range1955 1 58 58 BBa_I1060_sequence 1 ataaatgtgagcggataacattgacattgtgagcggataacaagatactgagcacgccaaaaatcaataatgagacaagatcatgtgcagtactaagaaaaagcctttgactcaggaacaactcgaagatgcccgccgtctcaaggccatctacgagaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z