LOCUS BBa_I1100 9369 bp DNA linear 25-Nov-2024 DEFINITION SBWB Ribosome Test System ACCESSION BBa_I1100 VERSION BBa_I1100.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 CDS 1219..7209 /label=BBa_C0101 stem_loop 7229..7272 /label=stem_loop misc_feature 7218..7297 /label=BBa_B0010 terminator 7218..7297 /label=BBa_B0010 stem_loop 7313..7332 /label=T7 TE misc_feature 7339..7339 /label=stop polyA_site 7333..7346 /label=polya misc_feature 7306..7346 /label=BBa_B0012 terminator 7306..7346 /label=BBa_B0012 terminator 7218..7346 /label=BBa_B0015 promoter 7369..7374 /label=-35 promoter 7379..7395 /label=OR1 promoter 7355..7371 /label=OR2 promoter 7392..7397 /label=-10 misc_feature 7355..7403 /label=BBa_R1051 promoter 7355..7403 /label=BBa_R1051 CDS 7410..8285 /label=araC misc_feature 8286..8318 /label=LVA misc_feature 8325..8349 /label=Help:Barcodes CDS 7410..8349 /label=BBa_C0080 stem_loop 8369..8412 /label=stem_loop misc_feature 8358..8437 /label=BBa_B0010 terminator 8358..8437 /label=BBa_B0010 stem_loop 8453..8472 /label=T7 TE misc_feature 8479..8479 /label=stop polyA_site 8473..8486 /label=polya misc_feature 8446..8486 /label=BBa_B0012 terminator 8446..8486 /label=BBa_B0012 terminator 8358..8486 /label=BBa_B0015 promoter 8509..8514 /label=-35 promoter 8519..8535 /label=OR1 promoter 8495..8511 /label=OR2 promoter 8532..8537 /label=-10 misc_feature 8495..8543 /label=BBa_R1051 promoter 8495..8543 /label=BBa_R1051 RBS 8552..8565 /label=BBa_B0035 CDS 8574..9232 /label=BBa_C0100 stem_loop 9252..9295 /label=stem_loop misc_feature 9241..9320 /label=BBa_B0010 terminator 9241..9320 /label=BBa_B0010 stem_loop 9336..9355 /label=T7 TE misc_feature 9362..9362 /label=stop polyA_site 9356..9369 /label=polya misc_feature 9329..9369 /label=BBa_B0012 terminator 9329..9369 /label=BBa_B0012 terminator 9241..9369 /label=BBa_B0015 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagaa attgaagagt ttgatcatgg ctcagattga acgctggcgg 1261 caggcctaac acatgcaagt cgaacggtaa caggaagaag cttgcttctt tgctgacgag 1321 tggcggacgg gtgagtaatg tctgggaaac tgcctgatgg agggggataa ctactggaaa 1381 cggtagctaa taccgcataa cgtcgcaaga ccaaagaggg ggaccttcgg gcctcttgcc 1441 atcggatgtg cccagatggg attagctagt aggtggggta acggctcacc taggcgacga 1501 tccctagctg gtctgagagg atgaccagcc acactggaac tgagacacgg tccagactcc 1561 tacgggaggc agcagtgggg aatattgcac aatgggcgca agcctgatgc agccatgccg 1621 cgtgtatgaa gaaggccttc gggttgtaaa gtactttcag cggggaggaa gggagtaaag 1681 ttaatacctt tgctcattga cgttacccgc agaagaagca ccggctaact ccgtgccagc 1741 agccgcggta atacggaggg tgcaagcgtt aatcggaatt actgggcgta aagcgcacgc 1801 aggcggtttg ttaagtcaga tgtgaaatcc ccgggctcaa cctgggaact gcatctgata 1861 ctggcaagct tgagtctcgt agaggggggt agaattccag gtgtagcggt gaaatgcgta 1921 gagatctgga ggaataccgg tggcgaaggc ggccccctgg acgaagactg acgctcaggt 1981 gcgaaagcgt ggggagcaaa caggattaga taccctggta gtccacgccg taaacgatgt 2041 cgacttggag gttgtgccct tgaggcgtgg cttccggagc taacgcgtta agtcgaccgc 2101 ctggggagta cggccgcaag gttaaaactc aaatgaattg acgggggccc gcacaagcgg 2161 tggagcatgt ggtttaattc gatgcaacgc gaagaacctt acctggtctt gacatccacg 2221 gaagttttca gagatgagaa tgtgccttcg ggaaccgtga gacaggtgct gcatggctgt 2281 cgtcagctcg tgttgtgaaa tgttgggtta agtcccgcaa cgagcgcaac ccttatcctt 2341 tgttgccagc ggtccggccg ggaactcaaa ggagactgcc agtgataaac tggaggaagg 2401 tggggatgac gtcaagtcat catggccctt acgaccaggg ctacacacgt gctacaatgg 2461 cgcatacaaa gagaagcgac ctcgcgagag caagcggacc tcataaagtg cgtcgtagtc 2521 cggattggag tctgcaactc gactccatga agtcggaatc gctagtaatc gtggatcaga 2581 atgccacggt gaatacgttc ccgggccttg tacacaccgc ccgtcacacc atgggagtgg 2641 gttgcaaaag aagtaggtag cttaaccttc gggagggcgc ttaccacttt gtgattcatg 2701 actggggtga agtcgtaaca aggtaaccgt aggggaacct gcggttggat cacctcctta 2761 ccttaaagaa gcgtactttg tagtgctcac acagattgtc tgatagaaag tgaaaagcaa 2821 ggcgtttacg cgttgggagt gaggctgaag agaataaggc cgttcgcttt ctattaatga 2881 aagctcaccc tacacgaaaa tatcacgcaa cgcgtgataa gcaattttcg tgtccccttc 2941 gtctagaggc ccaggacacc gccctttcac ggcggtaaca ggggttcgaa tcccctaggg 3001 gacgccactt gctggtttgt gagtgaaagt cgccgacctt aatatctcaa aactcatctt 3061 cgggtgatgt ttgagatatt tgctctttaa aaatctggat caagctgaaa attgaaacac 3121 tgaacaacga gagttgttcg tgagtctctc aaattttcgc aacacgatga tgaatcgaaa 3181 gaaacatctt cgggttgtga ggttaagcga ctaagcgtac acggtggatg ccctggcagt 3241 cagaggcgat gaaggacgtg ctaatctgcg ataagcgtcg gtaaggtgat atgaaccgtt 3301 ataaccggcg atttccgaat ggggaaaccc agtgtgtttc gacacactat cattaactga 3361 atccataggt taatgaggcg aaccggggga actgaaacat ctaagtaccc cgaggaaaag 3421 aaatcaaccg agattccccc agtagcggcg agcgaacggg gagcagccca gagcctgaat 3481 cagtgtgtgt gttagtggaa gcgtctggaa aggcgcgcga tacagggtga cagccccgta 3541 cacaaaaatg cacatgctgt gagctcgatg agtagggcgg gacacgtggt atcctgtctg 3601 aatatggggg gaccatcctc caaggctaaa tactcctgac tgaccgatag tgaaccagta 3661 ccgtgaggga aaggcgaaaa gaaccccggc gaggggagtg aaaaagaacc tgaaaccgtg 3721 tacgtacaag cagtgggagc acgcttaggc gtgtgactgc gtaccttttg tataatgggt 3781 cagcgactta tattctgtag caaggttaac cgaatagggg agccgaaggg aaaccgagtc 3841 ttaactgggc gttaagttgc agggtataga cccgaaaccc ggtgatctag ccatgggcag 3901 gttgaaggtt gggtaacact aactggagga ccgaaccgac taatgttgaa aaattagcgg 3961 atgacttgtg gctgggggtg aaaggccaat caaaccggga gatagctggt tctccccgaa 4021 agctatttag gtagcgcctc gtgaattcat ctccgggggt agagcactgt ttcggcaagg 4081 gggtcatccc gacttaccaa cccgatgcaa actgcgaata ccggagaatg ttatcacggg 4141 agacacacgg cgggtgctaa cgtccgtcgt gaagagggaa acaacccaga ccgccagcta 4201 aggtcccaaa gtcatggtta agtgggaaac gatgtgggaa ggcccagaca gccaggatgt 4261 tggcttagaa gcagccatca tttaaagaaa gcgtaatagc tcactggtcg agtcggcctg 4321 cgcggaagat gtaacggggc taaaccatgc accgaagctg cggcagcgac gcttatgcgt 4381 tgttgggtag gggagcgttc tgtaagcctg cgaaggtgtg ctgtgaggca tgctggaggt 4441 atcagaagtg cgaatgctga cataagtaac gataaagcgg gtgaaaagcc cgctcgccgg 4501 aagaccaagg gttcctgtcc aacgttaatc ggggcagggt gagtcgaccc ctaaggcgag 4561 gccgaaaggc gtagtcgatg ggaaacaggt taatattcct gtacttggtg ttactgcgaa 4621 ggggggacgg agaaggctat gttggccggg cgacggttgt cccggtttaa gcgtgtaggc 4681 tggttttcca ggcaaatccg gaaaatcaag gctgaggcgt gatgacgagg cactacggtg 4741 ctgaagcaac aaatgccctg cttccaggaa aagcctctaa gcatcaggta acatcaaatc 4801 gtaccccaaa ccgacacagg tggtcaggta gagaatacca aggcgcttga gagaactcgg 4861 gtgaaggaac taggcaaaat ggtgccgtaa cttcgggaga aggcacgctg atatgtaggt 4921 gaggtccctc gcggatggag ctgaaatcag tcgaagatac cagctggctg caactgttta 4981 ttaaaaacac agcactgtgc aaacacgaaa gtggacgtat acggtgtgac gcctgcccgg 5041 tgccggaagg ttaattgatg gggttagcgc aagcgaagct cttgatcgaa gccccggtaa 5101 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga 5161 cctgcacgaa tggcgtaatg atggccaggc tgtctccacc cgagactcag tgaaattgaa 5221 ctcgctgtga agatgcagtg tacccgcggc aagacggaaa gaccccgtga acctttacta 5281 tagcttgaca ctgaacattg agccttgatg tgtaggatag gtgggaggct ttgaagtgtg 5341 gacgccagtc tgcatggagc cgaccttgaa ataccaccct ttaatgtttg atgttctaac 5401 gttgacccgt aatccgggtt gcggacagtg tctggtgggt agtttgactg gggcggtctc 5461 ctcctaaaga gtaacggagg agcacgaagg ttggctaatc ctggtcggac atcaggaggt 5521 tagtgcaatg gcataagcca gcttgactgc gagcgtgacg gcgcgagcag gtgcgaaagc 5581 aggtcatagt gatccggtgg ttctgaatgg aagggccatc gctcaacgga taaaaggtac 5641 tccggggata acaggctgat accgcccaag agttcatatc gacggcggtg tttggcacct 5701 cgatgtcggc tcatcacatc ctggggctga agtaggtccc aagggtatgg ctgttcgcca 5761 tttaaagtgg tacgcgagct gggtttagaa cgtcgtgaga cagttcggtc cctatctgcc 5821 gtgggcgctg gagaactgag gggggctgct cctagtacga gaggaccgga gtggacgcat 5881 cactggtgtt cgggttgtca tgccaatggc actgcccggt agctaaatgc ggaagagata 5941 agtgctgaaa gcatctaagc acgaaacttg ccccgagatg agttctccct gaccctttaa 6001 gggtcctgaa ggaacgttga agacgacgac gttgataggc cgggtgtgta agcgcagcga 6061 tgcgttgagc taaccggtac taatgaaccg tgaggcttaa ccttacaacg ccgaagctgt 6121 tttggcggat gagagaagat tttcagcctg atacagatta aatcagaacg cagaagcggt 6181 ctgataaaac agaatttgcc tggcggcagt agcgcggtgg tcccacctga ccccatgccg 6241 aactcagaag tgaaacgccg tagcgccgat ggtagtgtgg ggtctcccca tgcgagagta 6301 gggaactgcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt 6361 tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgg gagcggattt 6421 gaacgttgcg aagcaacggc ccggagggtg gcgggcagga cgcccgccat aaactgccag 6481 gcatcaaatt aagcagaagg ccatcctgac ggatggcctt tttgcgtttc tacaaactct 6541 tcctgtcgtc atatctacaa gccatccccc cacagatacg gtaaactagc ctcgtttttg 6601 catcaggaaa gcagctatga accactcctt aaaaccctgg aacacatttg gcattgatca 6661 taatgctcag cacattgtat gtgccgaaga cgaacaacaa ttactcaatg cctggcagta 6721 tgcaaccgca gaaggacaac ccgttcttat tctgggtgaa ggaagtaatg tactttttct 6781 ggaggactat cgcggcacgg tgatcatcaa ccggatcaaa ggtatcgaaa ttcatgatga 6841 acctgatgcg tggtatttac atgtaggagc cggagaaaac tggcatcgtc tggtaaaata 6901 cactttgcag gaaggtatgc ctggtctgga aaatctggca ttaattcctg gttgtgtcgg 6961 ctcatcacct atccagaata ttggtgctta tggcgtagaa ttacagcgag tttgcgctta 7021 tgttgattct gttgaactgg cgacaggcaa gcaagtgcgc ttaactgcca aagagtgccg 7081 ttttggctat cgcgacagta tttttaaaca tgaataccag gaccgcttcg ctattgtagc 7141 cgtaggtctg cgtctgccaa aagagtggca acctgtacta acgtatggtg acttaactcg 7201 tctggatcct actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc 7261 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc 7321 ttcgggtggg cctttctgcg tttatatact agagtaacac cgtgcgtgtt gacaatttta 7381 cctctggcgg tgatactggt tgctactaga tggctgaagc gcaaaatgat cccctgctgc 7441 cgggatactc gtttaacgcc catctggtgg cgggtttaac gccgattgag gccaatggtt 7501 atctcgattt ttttatcgac cgaccgctgg gaatgaaagg ttatattctc aatctcacca 7561 ttcgcggtca gggggtggtg aaaaatcagg gacgagaatt tgtctgccga ccgggtgata 7621 ttttgctgtt cccgccagga gagattcatc actacggtcg tcatccggag gctcgcgaat 7681 ggtatcacca gtgggtttac tttcgtccgc gcgcctactg gcatgaatgg cttaactggc 7741 cgtcaatatt tgccaatacg ggtttctttc gcccggatga agcgcaccag ccgcatttca 7801 gcgacctgtt tgggcaaatc attaacgccg ggcaagggga agggcgctat tcggagctgc 7861 tggcgataaa tctgcttgag caattgttac tgcggcgcat ggaagcgatt aacgagtcgc 7921 tccatccacc gatggataat cgggtacgcg aggcttgtca gtacatcagc gatcacctgg 7981 cagacagcaa ttttgatatc gccagcgtcg cacagcatgt ttgcctgtcg ccgtcgcgtc 8041 tgtcacatct tttccgccag cagttaggga ttagcgtctt aagctggcgc gaggaccaac 8101 gcatcagcca ggcgaagctg cttttgagca ctacccggat gcctatcgcc accgtcggtc 8161 gcaatgttgg ttttgacgat caactctatt tctcgcgagt atttaaaaaa tgcaccgggg 8221 ccagcccgag cgagttccgt gccggttgtg aagaaaaagt gaatgatgta gccgtcaagt 8281 tgtcagctgc aaacgacgaa aactacgctt tagtagctta ataacactga tagtgctagt 8341 gtagatcact actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc 8401 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc 8461 ttcgggtggg cctttctgcg tttatatact agagtaacac cgtgcgtgtt gacaatttta 8521 cctctggcgg tgatactggt tgctactaga gattaaagag gagaatacta gagtacgccc 8581 cgccctgcca ctcatcgcag tactgttgta attcattaag cattctgccg acatggaagc 8641 catcacaaac ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg 8701 tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta 8761 aatcaaaact ggtgaaactc acccagggat tggctgagac gaaaaacata ttctcaataa 8821 accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt 8881 gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac gtttcagttt 8941 gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt 9001 tcattgccat acgaaattcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg 9061 ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct 9121 gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac 9181 gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc attactagag 9241 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt 9301 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct 9361 gcgtttata //