##gff-version 3
##sequence-region BBa_I1101 1 4589
BBa_I1101	.	terminator	1974	2014	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I1101	.	stem_loop	1981	2000	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I1101	.	polyA_site	2001	2014	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I1101	.	stop_codon	2007	2007	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I1101	.	engineered_region	1974	2014	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I1101	.	promoter	1	1210	.	+	0	ID=BBa_I0500;Name=BBa_I0500
BBa_I1101	.	promoter	1043	1114	.	+	0	ID=annotation1498842;Name=PC;Parent=BBa_I0500
BBa_I1101	.	promoter	1080	1210	.	+	0	ID=annotation1498841;Name=Pbad;Parent=BBa_I0500
BBa_I1101	.	sequence_feature	1	1079	.	+	0	ID=annotation1498843;Name=AraC;Parent=BBa_I0500
BBa_I1101	.	terminator	4549	4589	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I1101	.	stem_loop	4556	4575	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I1101	.	polyA_site	4576	4589	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I1101	.	stop_codon	4582	4582	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I1101	.	engineered_region	4549	4589	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I1101	.	terminator	4461	4540	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I1101	.	engineered_region	4461	4540	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I1101	.	stem_loop	4472	4515	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I1101	.	CDS	3026	3684	.	+	0	ID=BBa_C0100;Name=BBa_C0100
BBa_I1101	.	promoter	2023	2071	.	+	0	ID=BBa_R1051;Name=BBa_R1051
BBa_I1101	.	promoter	2047	2063	.	+	0	ID=annotation2076;Name=OR1;Parent=BBa_R1051
BBa_I1101	.	promoter	2060	2065	.	+	0	ID=annotation2078;Name=-10;Parent=BBa_R1051
BBa_I1101	.	engineered_region	2023	2071	.	+	0	ID=annotation7074;Name=BBa_R1051;Parent=BBa_R1051
BBa_I1101	.	promoter	2037	2042	.	+	0	ID=annotation2075;Name=-35;Parent=BBa_R1051
BBa_I1101	.	promoter	2023	2039	.	+	0	ID=annotation2077;Name=OR2;Parent=BBa_R1051
BBa_I1101	.	CDS	1219	1877	.	+	0	ID=BBa_C0100;Name=BBa_C0100
BBa_I1101	.	CDS	2078	2992	.	+	0	ID=BBa_C0080;Name=BBa_C0080
BBa_I1101	.	CDS	2078	2953	.	+	0	ID=annotation308191;Name=araC;Parent=BBa_C0080
BBa_I1101	.	tag	2954	2986	.	+	0	ID=annotation308196;Name=LVA;Parent=BBa_C0080
BBa_I1101	.	engineered_tag	2993	3017	.	+	0	ID=annotation2214004;Name=Help:Barcodes;Parent=BBa_C0080
BBa_I1101	.	terminator	1886	1965	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I1101	.	engineered_region	1886	1965	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I1101	.	stem_loop	1897	1940	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I1101	.	engineered_region	3691	4452	.	+	0	ID=BBa_E0032;Name=BBa_E0032
BBa_I1101	.	sequence_alteration	3932	3932	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_I1101	.	engineered_region	3691	4452	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_I1101	.	tag	4408	4446	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_I1101	.	CDS	3691	4452	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_I1101	.	stop_codon	4447	4452	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
>BBa_I1101
ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcat
tttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtgg
tgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaa
aagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtga
tcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgcc
gcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttg
cccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccag
ttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgac
cgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcac
caccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataa
ccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcg
cttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactg
cgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaa
gccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacac
tttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttc
tccatacccgtttttttgggctagctactagagtacgccccgccctgccactcatcgcagtactgttgtaattcattaa
gcattctgccgacatggaagccatcacaaacggcatgatgaacctgaatcgccagcggcatcagcaccttgtcgccttg
cgtataatatttgcccatggtgaaaacgggggcgaagaagttgtccatattggccacgtttaaatcaaaactggtgaaa
ctcacccagggattggctgagacgaaaaacatattctcaataaaccctttagggaaataggccaggttttcaccgtaac
acgccacatcttgcgaatatatgtgtagaaactgccggaaatcgtcgtggtattcactccagagcgatgaaaacgtttc
agtttgctcatggaaaacggtgtaacaagggtgaacactatcccatatcaccagctcaccgtctttcattgccatacga
aattccggatgagcattcatcaggcgggcaagaatgtgaataaaggccggataaaacttgtgcttatttttctttacgg
tctttaaaaaggccgtaatatccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatg
ttctttacgatgccattgggatatatcaacggtggtatatccagtgatttttttctccattactagagccaggcatcaa
ataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtc
acactggctcaccttcgggtgggcctttctgcgtttatatactagagtaacaccgtgcgtgttgacaattttacctctg
gcggtgatactggttgctactagatggctgaagcgcaaaatgatcccctgctgccgggatactcgtttaacgcccatct
ggtggcgggtttaacgccgattgaggccaatggttatctcgatttttttatcgaccgaccgctgggaatgaaaggttat
attctcaatctcaccattcgcggtcagggggtggtgaaaaatcagggacgagaatttgtctgccgaccgggtgatattt
tgctgttcccgccaggagagattcatcactacggtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactt
tcgtccgcgcgcctactggcatgaatggcttaactggccgtcaatatttgccaatacgggtttctttcgcccggatgaa
gcgcaccagccgcatttcagcgacctgtttgggcaaatcattaacgccgggcaaggggaagggcgctattcggagctgc
tggcgataaatctgcttgagcaattgttactgcggcgcatggaagcgattaacgagtcgctccatccaccgatggataa
tcgggtacgcgaggcttgtcagtacatcagcgatcacctggcagacagcaattttgatatcgccagcgtcgcacagcat
gtttgcctgtcgccgtcgcgtctgtcacatcttttccgccagcagttagggattagcgtcttaagctggcgcgaggacc
aacgcatcagccaggcgaagctgcttttgagcactacccggatgcctatcgccaccgtcggtcgcaatgttggttttga
cgatcaactctatttctcgcgagtatttaaaaaatgcaccggggccagcccgagcgagttccgtgccggttgtgaagaa
aaagtgaatgatgtagccgtcaagttgtcagctgcaaacgacgaaaactacgctttagtagcttaataacactgatagt
gctagtgtagatcactactagagtacgccccgccctgccactcatcgcagtactgttgtaattcattaagcattctgcc
gacatggaagccatcacaaacggcatgatgaacctgaatcgccagcggcatcagcaccttgtcgccttgcgtataatat
ttgcccatggtgaaaacgggggcgaagaagttgtccatattggccacgtttaaatcaaaactggtgaaactcacccagg
gattggctgagacgaaaaacatattctcaataaaccctttagggaaataggccaggttttcaccgtaacacgccacatc
ttgcgaatatatgtgtagaaactgccggaaatcgtcgtggtattcactccagagcgatgaaaacgtttcagtttgctca
tggaaaacggtgtaacaagggtgaacactatcccatatcaccagctcaccgtctttcattgccatacgaaattccggat
gagcattcatcaggcgggcaagaatgtgaataaaggccggataaaacttgtgcttatttttctttacggtctttaaaaa
ggccgtaatatccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctttacga
tgccattgggatatatcaacggtggtatatccagtgatttttttctccattactagatggtgagcaagggcgaggagct
gttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggc
gagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccc
tcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtc
cgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtg
aagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggc
acaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaactt
caagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggc
cccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcaca
tggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacga
cgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggc
ctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgc
gtttata