LOCUS BBa_I11062 2024 bp DNA linear 22-Dec-2024 DEFINITION HK022 half bit ACCESSION BBa_I11062 VERSION BBa_I11062.1 FEATURES Location/Qualifiers misc_feature 1..3 /label=spacer misc_feature 4..9 /label=KpnI misc_feature 10..12 /label=spacer RBS 13..18 /label=RBS misc_feature 19..23 /label=spacer CDS 24..1094 /label=Integrase HK022+LVA (BBa_I11040) misc_feature 1095..1127 /label=LVA misc_feature 1128..1133 /label=stop misc_feature 1134..1136 /label=spacer misc_feature 1137..1142 /label=AclI misc_feature 1143..1145 /label=spacer misc_feature 1146..1563 /label=P21 attP attachment site (BBa_I11053) misc_feature 1564..1566 /label=spacer misc_feature 1567..1572 /label=PvuI misc_feature 1573..1575 /label=spacer stem_loop 1576..1705 /label=B0025 reverse terminator misc_feature 1706..1708 /label=spacer misc_feature 1709..1714 /label=XmaI misc_feature 1715..1717 /label=spacer misc_feature 1718..1736 /label=P21 attB (I11053) misc_feature 1737..1739 /label=spacer misc_feature 1740..1745 /label=BmtI RBS 1746..1751 /label=RBS misc_feature 1752..1756 /label=spacer CDS 1757..1972 /label=cI HK022 misc_feature 1973..2005 /label=AAV misc_feature 2006..2011 /label=stop misc_feature 2012..2014 /label=spacer misc_feature 2015..2022 /label=NotI misc_feature 2021..2022 /label=spacer ORIGIN 1 atcggtaccg caaggaggtc atcatgggaa gaaggcgaag tcatgagcgc cgggatttac 61 cccctaacct ttatataaga aacaatggat attactgcta cagggaccca aggacgggta 121 aagagtttgg attaggccga gacaggcgaa tcgcaatcac tgaagctata caggccaaca 181 ttgagttgct atccgggaac aggcgtgagt cactgataga cagaattaaa ggcgctgacg 241 caatcactct tcatgcgtgg cttgaccgat atgaaacaat cctcagcgag aggggtatca 301 ggccgaaaac tctactcgac tacgccagca aaatcagagc aatccgaaga aaattgccgg 361 acaaaccgct cgctgacata tcaacgaaag aggtggcagc aatgctaaac acctacgtag 421 cagaaggcaa atcggcttcc gcaaaattga tcaggtcaac ccttgttgac gtttttcgtg 481 aggcaatagc cgaggggcat gtggctacga atccggtaac agcaacccgc acagcaaaat 541 cagaagtaag gcgttcaaga ctgacagcta atgaatatgt cgcgatatac catgcagccg 601 aaccgctccc aatctggctg agactggcaa tggatttggc tgtcgttaca gggcagagag 661 taggcgattt gtgcagaatg aaatggtcag acataaacga caaccatctt cacattgaac 721 agagtaaaac aggggctaaa ctcgccattc cactaacgct aacgattgac gcgctcaata 781 tctcattggc tgatacacta cagcaatgca gggaggccag cagcagtgaa actataatcg 841 catcaaagca tcacgatccg ctttccccga aaacagtatc aaagtatttt acaaaggcga 901 gaaatgcatc tggactctca tttgatggaa acccgccaac attccatgaa ctgcgtagcc 961 tgtcagcgag gctataccgg aaccagattg gcgataagtt tgctcaacgt cttctcgggc 1021 ataaatcaga ttcaatggcg gcgcggtata gggacagccg tggacgggaa tgggacaaaa 1081 ttgaaatcga caaagcagca aacgacgaaa actacgcttt agtagcttga tgagcaaacg 1141 ttcgaaaatc tttccccaaa ataaaaacga acgtcaatga aatcaaacgg ttgaataaag 1201 ttgattttgg ctaatacaaa gacaagaaaa taatatttat gattaaatat cagcgagttg 1261 aatacataat ttttatatac tgctgcgcca tatgggctgg actgaagccg cagacctgat 1321 tgttaaaggt atggaaggcg caatcaacgc caagaccgta acctatgact tcgaacgtct 1381 gatggaaggc gctaaactgc tgaaatgcag cgagtttggt gacgcgatca tcaaaaatat 1441 gtaattacca catgtgttaa atattataac gggcgtataa cacgcccgtt gttttatgat 1501 gatgtaaaat cttccccaaa actttcccca aaacccttcc ccaaaactgg ctattttcta 1561 tgcacacgat cggtatataa acgcagaaag gcccacccga aggtgagcca gtgtgactct 1621 aagtagagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc tttcgactga 1681 gcctttcgtt ttatttgatg cctggattcc cgggtcaata tggcgcagca tcatctcaag 1741 ctagcaggag gctaccatgt acttaacact tcaggagtgg aacgctcgcc agcgacgccc 1801 aagaagcctt gaaacagttc gtcgatgggt gcgcgaatgc aggatattcc ctcctccggt 1861 taaggatgga agagagtatc tgttccacga atcagcggta aaggttgact taaatcgacc 1921 agtaacaggt agccttttga agaggatcag aaatgggaag aaggcgaagt cagcagcaaa 1981 cgacgaaaac tacgctgctg ctgtttaata accggcggcc gcct //