LOCUS       BBa_I11063              1951 bp    DNA     linear       22-Dec-2024
DEFINITION  P21 half bit
ACCESSION   BBa_I11063
VERSION     BBa_I11063.1  
FEATURES             Location/Qualifiers
     misc_feature    1..3
                     /label=spacer
     misc_feature    4..9
                     /label=SalI
     misc_feature    10..12
                     /label=spacer
     RBS             13..18
                     /label=RBS
     misc_feature    19..23
                     /label=spacer
     CDS             24..1163
                     /label=Integrase p21 (BBa_I11050)
     misc_feature    1164..1196
                     /label=LVA
     misc_feature    1197..1202
                     /label=stop
     misc_feature    1203..1205
                     /label=spacer
     misc_feature    1206..1211
                     /label=SacII
     misc_feature    1212..1214
                     /label=spacer
     misc_feature    1215..1443
                     /label=HK022 attP attachment site (BBa_I11043)
     misc_feature    1444..1446
                     /label=spacer
     misc_feature    1447..1452
                     /label=MluI
     misc_feature    1453..1455
                     /label=spacer
     misc_feature    1456..1584
                     /label=BBa_I11013 terminator
     misc_feature    1585..1587
                     /label=spacer
     misc_feature    1588..1593
                     /label=ApaI
     misc_feature    1594..1596
                     /label=spacer
     misc_feature    1597..1647
                     /label=HK022 attB rev comp (BBa_I11042)
     misc_feature    1648..1650
                     /label=spacer
     misc_feature    1651..1656
                     /label=BamHI
     RBS             1657..1662
                     /label=RBS
     misc_feature    1663..1667
                     /label=spacer
     CDS             1668..1901
                     /label=Excisionase p21 (BBa_I11051)
     misc_feature    1902..1934
                     /label=AAV
     misc_feature    1935..1940
                     /label=stop
     misc_feature    1941..1943
                     /label=spacer
     misc_feature    1944..1949
                     /label=XbaI
     misc_feature    1950..1951
                     /label=spacer
ORIGIN
        1 atcgtcgacg caaggaggtc atcatggctg ctagaccccg atctcacaaa atctctatac
       61 ccaatttata ttgcaaatta gataagcgaa ccggaaaggt atattggcaa tacaaacatc
      121 cactatccgg tcgttttcat agcttaggaa ctgatgagaa tgaagcaaaa caagttgcta
      181 ctgaagcaaa taccattatt gctgaacaac gtaccagaca aatattaagc gtcaatgagc
      241 gtctggaaag aatgaaaggc aggcgctcag acattacggt gacagaatgg cttgataaat
      301 atatttctat ccaggaggac aggctgcaac ataatgaact aagacccaac tcctatcggc
      361 aaaaaggcaa acccattcgt cttttccgtg agcattgtgg aatgcaacac ctcaaggata
      421 ttaccgcact tgatattgcc gaaataattg atgctgtaaa ggctgaaggt cataacagga
      481 tggcgcaagt cgtgagaatg gtgttgatcg acgtcttcaa agaagcacaa cacgcaggac
      541 atgttccgcc aggatttaac ccagcgcagg caacaaaaca accgcgaaat cgagtaaacc
      601 gccaaagatt gtcactgctc gaatggcagg caatatttga aagcgtaagc agacggcagc
      661 cctatttaaa atgcggcatg ctacttgctc ttgttactgg acagcgttta ggcgatatct
      721 gcaatttgaa attctctgat atatgggacg acatgttgca cattactcag gaaaaaaccg
      781 gttcaaaact tgctattccg cttaacctga aatgcgatgc tctgaatatt acccttcgtg
      841 aagttatatc tcagtgcagg gatgctgttg ttagtaaata tctggtccat taccgtcaca
      901 ctacctctca agcaaacaga ggagaccagg tttctgcaaa tactctgaca acggctttta
      961 aaaaggccaa ggaaaaatgt ggcataaaat gggagcaagg aactgcgccc acatttcatg
     1021 aacagcgatc tctgtcagaa cgattatatc gggaacaggg tctggatacg caaaagttgt
     1081 taggccataa atccagaaaa atgaccgacc gatacaatga tgatcgtggt aaagactgga
     1141 ttatcgtaga tatcaaaaca gcagcagcaa acgacgaaaa ctacgcttta gtagcttagt
     1201 aggcaccgcg gcgaaggtca ctaatactat ctaagtagtt gattcatagt gactggatat
     1261 gttgcgtttt gtcgcattat gtagtctatc atttaaccac agattagtgt aatgcgatga
     1321 tttttaagtg attaatgtta ttttgtcatc ctttaggtga ataagttgta tatttaaaat
     1381 ctctttaatt atcagtaaat taatgtaagt aggtcattat tagtcaaaat aaaatcattt
     1441 gtcacaacgc gtgtaccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
     1501 ttcgttttat ctgttgtttg tttatgaacg ctctctacta gagtcacact ggctcacctt
     1561 cgggtgggcc tttctgcgtt tatacgtggg ccctcaattc cgctttgcga ctcaaccttt
     1621 ttcacctaaa gtgcaccgac cgtgaatcaa ggatccagga ggtcctcatg tctcgactaa
     1681 tcactttaca ggactgggct aaagaagaat ttggggactt agcaccaagt gagcgagttc
     1741 tgaaaaaata cgcgcaaggg aaaatgatgg ccccacccgc tataaaagtt ggtcgctact
     1801 ggatgattga ccgaaattcc cgttttgtag gaacgcttgc agaaccgcaa ctcccaataa
     1861 acgcaaaccc aaaactccaa cggataatcg ctgatggctg cgcagcaaac gacgaaaact
     1921 acgctgctgc tgtttaataa ggatctagac c
//