LOCUS       BBa_I12002              1018 bp    DNA     linear       17-May-2024
DEFINITION  Barkai-Leibler design experiment part B (434cI)
ACCESSION   BBa_I12002
VERSION     BBa_I12002.1  
FEATURES             Location/Qualifiers
     misc_feature    1..41
                     /label=B0012
     misc_feature    50..129
                     /label=B0010
     terminator      1..129
                     /label=BBa_B0025
     misc_feature    146..162
                     /label=OR1
     misc_feature    170..186
                     /label=OR2
     promoter        185..190
                     /label=-35
     misc_feature    196..202
                     /label=mutate to obliterate OR3
     promoter        208..213
                     /label=-10
     promoter        138..219
                     /label=BBa_I12007
     misc_feature    232..235
                     /label=conserved
     RBS             228..239
                     /label=BBa_B0034
     CDS             246..914
                     /label=cI 434
     promoter        246..881
                     /label=BBa_I12014
     stem_loop       901..944
                     /label=stem_loop
     misc_feature    890..969
                     /label=BBa_B0010
     terminator      890..969
                     /label=BBa_B0010
     stem_loop       985..1004
                     /label=T7 TE
     misc_feature    1011..1011
                     /label=stop
     polyA_site      1005..1018
                     /label=polya
     misc_feature    978..1018
                     /label=BBa_B0012
     terminator      978..1018
                     /label=BBa_B0012
     terminator      890..1018
                     /label=BBa_B0015
ORIGIN
        1 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca
       61 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt
      121 gatgcctggt actagaggca accattatca ccgccagagg taaaatagtc aacacgcacg
      181 gtgttagata tttataaata gtggtgatag atttaacgtt actagagaaa gaggagaaat
      241 actagatgag tatttcttcc agggtaaaaa gcaaaagaat ccagcttgga cttaaccagg
      301 ctgaacttgc tcaaaaggtg gggactaccc agcagtctat agagcagctc gaaaacggta
      361 aaactaagcg accacgcttt ttaccagaac ttgcgtcagc tcttggcgta agtgttgact
      421 ggctgctcaa tggcacctct gattcgaatg ttagatttgt tgggcacgtt gagcccaaag
      481 ggaaatatcc attgattagc atggttagag ctggttcgtg gtgtgaagct tgtgaaccct
      541 acgatatcaa ggacattgat gaatggtatg acagtgacgt taacttatta ggcaatggat
      601 tctggctgaa ggttgaaggt gattccatga cctcacctgt aggtcaaagc atccctgaag
      661 gtcatatggt gttagtagat actggacggg agccagtgaa tggaagcctt gttgtagcca
      721 aactgactga cgcgaacgaa gcaacattca agaaactggt catagatggc ggtcagaagt
      781 acctgaaagg cctgaatcct tcatggccta tgactcctat caacggaaac tgcaagatta
      841 tcggtgttgt cgtggaagcg agggtaaaat tcgtataata atactagagc caggcatcaa
      901 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
      961 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//