LOCUS       BBa_I12012              1111 bp    DNA     linear       25-Nov-2024
DEFINITION  Reporter for the Barkai-Leibler oscillator
ACCESSION   BBa_I12012
VERSION     BBa_I12012.1  
FEATURES             Location/Qualifiers
     promoter        38..43
                     /label=-10
     promoter        15..20
                     /label=-35
     misc_feature    25..41
                     /label=OR1
     misc_feature    1..17
                     /label=OR2
     misc_feature    1..49
                     /label=BBa_R0051
     promoter        1..49
                     /label=BBa_R0051
     misc_feature    62..65
                     /label=conserved
     RBS             58..69
                     /label=BBa_B0034
     CDS             76..837
                     /label=YFP (LVA)
     misc_feature    832..837
                     /label=2
     misc_feature    793..831
                     /label=SsrA
     misc_feature    317..317
                     /label=A (Q->L)
     misc_feature    76..837
                     /label=BBa_E0032
     misc_feature    76..837
                     /label=BBa_E0032
     stem_loop       857..900
                     /label=stem_loop
     misc_feature    846..925
                     /label=BBa_B0010
     terminator      846..925
                     /label=BBa_B0010
     stem_loop       941..960
                     /label=T7 TE
     misc_feature    967..967
                     /label=stop
     polyA_site      961..974
                     /label=polya
     misc_feature    934..974
                     /label=BBa_B0012
     terminator      934..974
                     /label=BBa_B0012
     misc_feature    58..974
                     /label=BBa_E0432
     stem_loop       994..1037
                     /label=stem_loop
     misc_feature    983..1062
                     /label=BBa_B0010
     terminator      983..1062
                     /label=BBa_B0010
     stem_loop       1078..1097
                     /label=T7 TE
     misc_feature    1104..1104
                     /label=stop
     polyA_site      1098..1111
                     /label=polya
     misc_feature    1071..1111
                     /label=BBa_B0012
     terminator      1071..1111
                     /label=BBa_B0012
     terminator      983..1111
                     /label=BBa_B0015
ORIGIN
        1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagaaa
       61 gaggagaaat actagatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
      121 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
      181 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
      241 gtgccctggc ccaccctcgt gaccaccttc ggctacggcc tgcaatgctt cgcccgctac
      301 cccgaccaca tgaagctgca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
      361 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
      421 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
      481 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
      541 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
      601 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
      661 ctgcccgaca accactacct gagctaccag tccgccctga gcaaagaccc caacgagaag
      721 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
      781 gagctgtaca agaggcctgc tgcaaacgac gaaaactacg ctttagtagc ttaataatac
      841 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
      901 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc
      961 tttctgcgtt tatatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac
     1021 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc
     1081 tcaccttcgg gtgggccttt ctgcgtttat a
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