BBa_I12018
type: ComponentDefinition
persistent identity: BBa I12018
display id: BBa_I12018
version: 1
was generated by: iGEM to SBOL conversion
description: Barkai-Leibler Relaxation Oscillator
created: 2004-07-21T11:00:00Z
modified: 2015-08-31T04:07:31Z
owned by: james
owned by: myers
mutable description: Modified Design
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 147
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: mcnamara
type: DnaRegion
role: partType/Project
role: SO:0000804
component: BBa_I12016
component: BBa_I12015
component: BBa_I12017
sequence annotation: BBa_I12015
sequence annotation: BBa_I12016
sequence annotation: BBa_I12017
sequence: https://synbiohub.org/public/igem/BBa_I12018_sequence/1

BBa_I12016
type: Component
persistent identity: component2231609
display id: component2231609
version: 1
top level: BBa_I12018
definition: BBa_I12016
access: public

BBa_I12015
type: Component
persistent identity: component2231591
display id: component2231591
version: 1
top level: BBa_I12018
definition: BBa_I12015
access: public

BBa_I12017
type: Component
persistent identity: component2231620
display id: component2231620
version: 1
top level: BBa_I12018
definition: BBa_I12017
access: public

BBa_I12015
type: SequenceAnnotation
persistent identity: annotation2231591
display id: annotation2231591
version: 1
top level: BBa_I12018
location: https://synbiohub.org/public/igem/BBa_I12018/annotation2231591/range2231591/1
component: BBa_I12015

https://synbiohub.org/public/igem/BBa_I12018/annotation2231591/range2231591/1
type: Range
persistent identity: range2231591
display id: range2231591
version: 1
top level: BBa_I12018
direction: direction/reverse
start: 1
end: 219
orientation: inline

BBa_I12016
type: SequenceAnnotation
persistent identity: annotation2231609
display id: annotation2231609
version: 1
top level: BBa_I12018
location: https://synbiohub.org/public/igem/BBa_I12018/annotation2231609/range2231609/1
component: BBa_I12016

https://synbiohub.org/public/igem/BBa_I12018/annotation2231609/range2231609/1
type: Range
persistent identity: range2231609
display id: range2231609
version: 1
top level: BBa_I12018
direction: direction/reverse
start: 228
end: 1247
orientation: inline

BBa_I12017
type: SequenceAnnotation
persistent identity: annotation2231620
display id: annotation2231620
version: 1
top level: BBa_I12018
location: https://synbiohub.org/public/igem/BBa_I12018/annotation2231620/range2231620/1
component: BBa_I12017

https://synbiohub.org/public/igem/BBa_I12018/annotation2231620/range2231620/1
type: Range
persistent identity: range2231620
display id: range2231620
version: 1
top level: BBa_I12018
direction: direction/reverse
start: 1256
end: 2046
orientation: inline

BBa_I12015
type: ComponentDefinition
persistent identity: BBa I12015
display id: BBa_I12015
version: 1
was generated by: iGEM to SBOL conversion
description: Protein Receiver (434; lambda)
created: 2004-07-21T11:00:00Z
modified: 2015-08-31T04:07:31Z
owned by: james
owned by: myers
mutable description: phage 434 represses and lambda activates
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 147
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: mcnamara
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_I12006
component: BBa_B0025
sequence annotation: BBa_B0025
sequence annotation: BBa_I12006
sequence: https://synbiohub.org/public/igem/BBa_I12015_sequence/1

BBa_I12006
type: Component
persistent identity: component2218164
display id: component2218164
version: 1
top level: BBa_I12015
definition: Prm +
access: public

BBa_B0025
type: Component
persistent identity: component2218158
display id: component2218158
version: 1
top level: BBa_I12015
definition: BBa_B0025
access: public

BBa_B0025
type: SequenceAnnotation
persistent identity: annotation2218158
display id: annotation2218158
version: 1
top level: BBa_I12015
location: https://synbiohub.org/public/igem/BBa_I12015/annotation2218158/range2218158/1
component: BBa_B0025

https://synbiohub.org/public/igem/BBa_I12015/annotation2218158/range2218158/1
type: Range
persistent identity: range2218158
display id: range2218158
version: 1
top level: BBa_I12015
direction: direction/reverse
start: 1
end: 129
orientation: inline

BBa_I12006
type: SequenceAnnotation
persistent identity: annotation2218164
display id: annotation2218164
version: 1
top level: BBa_I12015
location: https://synbiohub.org/public/igem/BBa_I12015/annotation2218164/range2218164/1
component: BBa_I12006

https://synbiohub.org/public/igem/BBa_I12015/annotation2218164/range2218164/1
type: Range
persistent identity: range2218164
display id: range2218164
version: 1
top level: BBa_I12015
direction: direction/reverse
start: 138
end: 219
orientation: inline

BBa_I12016
type: ComponentDefinition
persistent identity: BBa I12016
display id: BBa_I12016
version: 1
was generated by: iGEM to SBOL conversion
description: Amplifier
created: 2004-07-21T11:00:00Z
modified: 2015-08-31T04:07:31Z
owned by: james
owned by: myers
mutable description: Amplifies TIPS signal and produces lambda.
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 147
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: mcnamara
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_C0051
component: BBa_B0034
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_C0051
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I12016_sequence/1

BBa_C0051
type: Component
persistent identity: component946573
display id: component946573
version: 1
top level: BBa_I12016
definition: cI lam
access: public

BBa_B0034
type: Component
persistent identity: component946563
display id: component946563
version: 1
top level: BBa_I12016
definition: BBa_B0034
access: public

BBa_B0012
type: Component
persistent identity: component946589
display id: component946589
version: 1
top level: BBa_I12016
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component946579
display id: component946579
version: 1
top level: BBa_I12016
definition: BBa_B0010
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation946563
display id: annotation946563
version: 1
top level: BBa_I12016
location: https://synbiohub.org/public/igem/BBa_I12016/annotation946563/range946563/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I12016/annotation946563/range946563/1
type: Range
persistent identity: range946563
display id: range946563
version: 1
top level: BBa_I12016
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation946573
display id: annotation946573
version: 1
top level: BBa_I12016
location: https://synbiohub.org/public/igem/BBa_I12016/annotation946573/range946573/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_I12016/annotation946573/range946573/1
type: Range
persistent identity: range946573
display id: range946573
version: 1
top level: BBa_I12016
direction: direction/forward
start: 19
end: 768
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation946579
display id: annotation946579
version: 1
top level: BBa_I12016
location: https://synbiohub.org/public/igem/BBa_I12016/annotation946579/range946579/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I12016/annotation946579/range946579/1
type: Range
persistent identity: range946579
display id: range946579
version: 1
top level: BBa_I12016
direction: direction/forward
start: 802
end: 881
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation946589
display id: annotation946589
version: 1
top level: BBa_I12016
location: https://synbiohub.org/public/igem/BBa_I12016/annotation946589/range946589/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I12016/annotation946589/range946589/1
type: Range
persistent identity: range946589
display id: range946589
version: 1
top level: BBa_I12016
direction: direction/forward
start: 890
end: 930
orientation: inline

BBa_I12017
type: ComponentDefinition
persistent identity: BBa I12017
display id: BBa_I12017
version: 1
was generated by: iGEM to SBOL conversion
description: Protein Generator (434, -LVA)
created: 2004-07-21T11:00:00Z
modified: 2015-08-31T04:07:31Z
owned by: james
owned by: myers
mutable description: Produces 434 cI without an LVA tag
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 147
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: mcnamara
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_B0012
component: BBa_B0010
component: BBa_I12014
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_I12014
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I12017_sequence/1

BBa_B0012
type: Component
persistent identity: component1021664
display id: component1021664
version: 1
top level: BBa_I12017
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1021654
display id: component1021654
version: 1
top level: BBa_I12017
definition: BBa_B0010
access: public

BBa_I12014
type: Component
persistent identity: component1021649
display id: component1021649
version: 1
top level: BBa_I12017
definition: cI 434
access: public

BBa_B0034
type: Component
persistent identity: component1021644
display id: component1021644
version: 1
top level: BBa_I12017
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1021644
display id: annotation1021644
version: 1
top level: BBa_I12017
location: https://synbiohub.org/public/igem/BBa_I12017/annotation1021644/range1021644/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I12017/annotation1021644/range1021644/1
type: Range
persistent identity: range1021644
display id: range1021644
version: 1
top level: BBa_I12017
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_I12014
type: SequenceAnnotation
persistent identity: annotation1021649
display id: annotation1021649
version: 1
top level: BBa_I12017
location: https://synbiohub.org/public/igem/BBa_I12017/annotation1021649/range1021649/1
component: BBa_I12014

https://synbiohub.org/public/igem/BBa_I12017/annotation1021649/range1021649/1
type: Range
persistent identity: range1021649
display id: range1021649
version: 1
top level: BBa_I12017
direction: direction/forward
start: 19
end: 654
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1021654
display id: annotation1021654
version: 1
top level: BBa_I12017
location: https://synbiohub.org/public/igem/BBa_I12017/annotation1021654/range1021654/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I12017/annotation1021654/range1021654/1
type: Range
persistent identity: range1021654
display id: range1021654
version: 1
top level: BBa_I12017
direction: direction/forward
start: 663
end: 742
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1021664
display id: annotation1021664
version: 1
top level: BBa_I12017
location: https://synbiohub.org/public/igem/BBa_I12017/annotation1021664/range1021664/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I12017/annotation1021664/range1021664/1
type: Range
persistent identity: range1021664
display id: range1021664
version: 1
top level: BBa_I12017
direction: direction/forward
start: 751
end: 791
orientation: inline

cI 434
type: ComponentDefinition
persistent identity: BBa I12014
display id: BBa_I12014
version: 1
was generated by: iGEM to SBOL conversion
description: Repressor, 434 cI (RBS- LVA-)
created: 2004-07-20T11:00:00Z
modified: 2015-08-31T04:07:31Z
mutable provenance: Nikolnikov,S., Posfai,G. and Sain,B. "The construction of a versatile plasmid vector that allows direct selection of fragments cloned into six unique sites of the cI gene of coliphage 434"
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The 434 cI repressor protein coding sequence is a 710 base-pair sequence with the standard RBS-compatible BioBrick prefix and the standard BioBrick suffix sections on its ends. It binds to the 434 regulatory sequence, BBa_R0052. The sequence has no LVA tag nor RBS.
discontinued: false
dominant: true
experience: experience/None
group u list: _3_
m user id: 0
owner id: 148
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Essentially the same as BBa_C0052 but with the LVA tag removed.
star: false
creator: ryhsiao
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: cI 434
sequence: https://synbiohub.org/public/igem/BBa_I12014_sequence/1

cI 434
type: SequenceAnnotation
persistent identity: annotation912810
display id: annotation912810
version: 1
top level: cI 434
location: https://synbiohub.org/public/igem/BBa_I12014/annotation912810/range912810/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I12014/annotation912810/range912810/1
type: Range
persistent identity: range912810
display id: range912810
version: 1
top level: cI 434
direction: direction/forward
start: 1
end: 669
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

cI lam
type: ComponentDefinition
persistent identity: BBa C0051
display id: BBa_C0051
version: 1
was generated by: iGEM to SBOL conversion
description: cI repressor from E. coli phage lambda (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999).
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the cI repressor based on cI repressor from bacteriophage lambda modified with an LVA tail for rapid degradation of the protein. cI repressor binds to the cI regulator (BBa_R0051).
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001 References (unparsed) here: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001 BBa_C0051 cI repressor is based on the cI repressor from the Elowitz's repressilator. It has been modified to include a rapid degradation LAA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cI lambda
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0051_sequence/1

cI lambda
type: SequenceAnnotation
persistent identity: annotation23334
display id: annotation23334
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation23334/range23334/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0051/annotation23334/range23334/1
type: Range
persistent identity: range23334
display id: range23334
version: 1
top level: cI lam
direction: direction/forward
start: 4
end: 711
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation23335
display id: annotation23335
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation23335/range23335/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0051/annotation23335/range23335/1
type: Range
persistent identity: range23335
display id: range23335
version: 1
top level: cI lam
direction: direction/forward
start: 712
end: 744
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213991
display id: annotation2213991
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation2213991/range2213991/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0051/annotation2213991/range2213991/1
type: Range
persistent identity: range2213991
display id: range2213991
version: 1
top level: cI lam
direction: direction/forward
start: 751
end: 775
orientation: inline

BBa_B0025
type: ComponentDefinition
persistent identity: BBa B0025
display id: BBa_B0025
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0015), reversed
created: 2003-12-02T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: -- No description --
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Caitlin Conboy
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: B0012
sequence annotation: B0010
sequence: https://synbiohub.org/public/igem/BBa_B0025_sequence/1

B0012
type: SequenceAnnotation
persistent identity: annotation369702
display id: annotation369702
version: 1
top level: BBa_B0025
location: https://synbiohub.org/public/igem/BBa_B0025/annotation369702/range369702/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0025/annotation369702/range369702/1
type: Range
persistent identity: range369702
display id: range369702
version: 1
top level: BBa_B0025
direction: direction/reverse
start: 1
end: 41
orientation: inline

B0010
type: SequenceAnnotation
persistent identity: annotation369703
display id: annotation369703
version: 1
top level: BBa_B0025
location: https://synbiohub.org/public/igem/BBa_B0025/annotation369703/range369703/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0025/annotation369703/range369703/1
type: Range
persistent identity: range369703
display id: range369703
version: 1
top level: BBa_B0025
direction: direction/reverse
start: 50
end: 129
orientation: inline

Prm +
type: ComponentDefinition
persistent identity: BBa I12006
display id: BBa_I12006
version: 1
was generated by: iGEM to SBOL conversion
description: Modified lamdba Prm promoter (repressed by 434 cI)
created: 2004-07-13T11:00:00Z
modified: 2015-08-31T04:07:31Z
mutable provenance: Bushman(1993), Shih & Gussin (1983)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Lamdba Prm promoter modified to be activated by lamda repressor (cI) and repressed by 434 repressor (cI)
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 147
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The O-R1 region of 434 contained 14 base pairs as opposed to the 17 base pairs of the O-R3 site of lambda. Also, it was noticed that the O-R3 site of the lambda included part of the -10 site. Hence, to preserve the spacing and the -10 site, the three nucleotides that were in both the -10 site and the lambda O-R3 site were retained. The 14 nucleotides that were in the O-R3 site and not in the -10 site were replaced with the O-R1 site of the 434.
star: true
creator: mcnamara
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OR1 lambda
sequence annotation: OR2 lambda
sequence annotation: -35
sequence annotation: OR1 434
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_I12006_sequence/1

OR1 lambda
type: SequenceAnnotation
persistent identity: annotation786500
display id: annotation786500
version: 1
top level: Prm +
location: https://synbiohub.org/public/igem/BBa_I12006/annotation786500/range786500/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_I12006/annotation786500/range786500/1
type: Range
persistent identity: range786500
display id: range786500
version: 1
top level: Prm +
direction: direction/forward
start: 9
end: 25
orientation: inline

OR2 lambda
type: SequenceAnnotation
persistent identity: annotation786518
display id: annotation786518
version: 1
top level: Prm +
location: https://synbiohub.org/public/igem/BBa_I12006/annotation786518/range786518/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_I12006/annotation786518/range786518/1
type: Range
persistent identity: range786518
display id: range786518
version: 1
top level: Prm +
direction: direction/forward
start: 33
end: 49
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation837284
display id: annotation837284
version: 1
top level: Prm +
location: https://synbiohub.org/public/igem/BBa_I12006/annotation837284/range837284/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I12006/annotation837284/range837284/1
type: Range
persistent identity: range837284
display id: range837284
version: 1
top level: Prm +
direction: direction/forward
start: 48
end: 53
orientation: inline

OR1 434
type: SequenceAnnotation
persistent identity: annotation786365
display id: annotation786365
version: 1
top level: Prm +
location: https://synbiohub.org/public/igem/BBa_I12006/annotation786365/range786365/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_I12006/annotation786365/range786365/1
type: Range
persistent identity: range786365
display id: range786365
version: 1
top level: Prm +
direction: direction/forward
start: 56
end: 69
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation837228
display id: annotation837228
version: 1
top level: Prm +
location: https://synbiohub.org/public/igem/BBa_I12006/annotation837228/range837228/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I12006/annotation837228/range837228/1
type: Range
persistent identity: range837228
display id: range837228
version: 1
top level: Prm +
direction: direction/forward
start: 71
end: 76
orientation: inline

https://synbiohub.org/public/igem/BBa_I12018_sequence/1
type: Sequence
persistent identity: BBa I12018 sequence
display id: BBa_I12018_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggtactagaggcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttagatattacaaactttcttgtatagatttaacgttactagagaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttagatatttataaatagtggtgatagatttaacgttactagagaaagaggagaaatactagatgagtatttcttccagggtaaaaagcaaaagaatccagcttggacttaaccaggctgaacttgctcaaaaggtggggactacccagcagtctatagagcagctcgaaaacggtaaaactaagcgaccacgctttttaccagaacttgcgtcagctcttggcgtaagtgttgactggctgctcaatggcacctctgattcgaatgttagatttgttgggcacgttgagcccaaagggaaatatccattgattagcatggttagagctggttcgtggtgtgaagcttgtgaaccctacgatatcaaggacattgatgaatggtatgacagtgacgttaacttattaggcaatggattctggctgaaggttgaaggtgattccatgacctcacctgtaggtcaaagcatccctgaaggtcatatggtgttagtagatactggacgggagccagtgaatggaagccttgttgtagccaaactgactgacgcgaacgaagcaacattcaagaaactggtcatagatggcggtcagaagtacctgaaaggcctgaatccttcatggcctatgactcctatcaacggaaactgcaagattatcggtgttgtcgtggaagcgagggtaaaattcgtataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctac…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I12015_sequence/1
type: Sequence
persistent identity: BBa I12015 sequence
display id: BBa_I12015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggtactagaggcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttagatattacaaactttcttgtatagatttaacgt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I12016_sequence/1
type: Sequence
persistent identity: BBa I12016 sequence
display id: BBa_I12016_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttagatatttataaatagtggtgatagatttaacgt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I12017_sequence/1
type: Sequence
persistent identity: BBa I12017 sequence
display id: BBa_I12017_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgagtatttcttccagggtaaaaagcaaaagaatccagcttggacttaaccaggctgaacttgctcaaaaggtggggactacccagcagtctatagagcagctcgaaaacggtaaaactaagcgaccacgctttttaccagaacttgcgtcagctcttggcgtaagtgttgactggctgctcaatggcacctctgattcgaatgttagatttgttgggcacgttgagcccaaagggaaatatccattgattagcatggttagagctggttcgtggtgtgaagcttgtgaaccctacgatatcaaggacattgatgaatggtatgacagtgacgttaacttattaggcaatggattctggctgaaggttgaaggtgattccatgacctcacctgtaggtcaaagcatccctgaaggtcatatggtgttagtagatactggacgggagccagtgaatggaagccttgttgtagccaaactgactgacgcgaacgaagcaacattcaagaaactggtcatagatggcggtcagaagtacctgaaaggcctgaatccttcatggcctatgactcctatcaacggaaactgcaagattatcggtgttgtcgtggaagcgagggtaaaattcgtataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I12014_sequence/1
type: Sequence
persistent identity: BBa I12014 sequence
display id: BBa_I12014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagtatttcttccagggtaaaaagcaaaagaatccagcttggacttaaccaggctgaacttgctcaaaaggtggggactacccagcagtctatagagcagctcgaaaacggtaaaactaagcgaccacgctttttaccagaacttgcgtcagctcttggcgtaagtgttgactggctgctcaatggcacctctgattcgaatgttagatttgttgggcacgttgagcccaaagggaaatatccattgattagcatggttagagctggttcgtggtgtgaagcttgtgaaccctacgatatcaaggacattgatgaatggtatgacagtgacgttaacttattaggcaatggattctggctgaaggttgaaggtgattccatgacctcacctgtaggtcaaagcatccctgaaggtcatatggtgttagtagatactggacgggagccagtgaatggaagccttgttgtagccaaactgactgacgcgaacgaagcaacattcaagaaactggtcatagatggcggtcagaagtacctgaaaggcctgaatccttcatggcctatgactcctatcaacggaaactgcaagattatcggtgttgtcgtggaagcgagggtaaaattcgtataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0051_sequence/1
type: Sequence
persistent identity: BBa C0051 sequence
display id: BBa_C0051_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0025_sequence/1
type: Sequence
persistent identity: BBa B0025 sequence
display id: BBa_B0025_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctgg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I12006_sequence/1
type: Sequence
persistent identity: BBa I12006 sequence
display id: BBa_I12006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttagatattacaaactttcttgtatagatttaacgt
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z