LOCUS BBa_I12020 5176 bp DNA linear 07-Dec-2025 DEFINITION Test Construct of BBa_I12006 promoter ACCESSION BBa_I12020 VERSION BBa_I12020.1 FEATURES Location/Qualifiers misc_feature 1..3 /label=OR3 misc_feature 11..28 /label=OR2 misc_feature 34..51 /label=OR1 promoter 18..23 /label=-35 promoter 8..13 /label=-35 promoter 30..35 /label=-10 misc_feature 1..54 /label=BBa_R0053 promoter 1..54 /label=BBa_R0053 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 misc_feature 1170..1208 /label=LVA CDS 81..1208 /label=lacI-LVA misc_feature 81..1208 /label=BBa_C0012 misc_feature 1209..1233 /label=Help:Barcodes CDS 81..1208 /label=BBa_C0012 stem_loop 1253..1296 /label=stem_loop misc_feature 1242..1321 /label=BBa_B0010 terminator 1242..1321 /label=BBa_B0010 stem_loop 1337..1356 /label=T7 TE misc_feature 1363..1363 /label=stop polyA_site 1357..1370 /label=polya misc_feature 1330..1370 /label=BBa_B0012 terminator 1330..1370 /label=BBa_B0012 terminator 1242..1370 /label=BBa_B0015 misc_feature 1381..1397 /label=lac O1 promoter 1398..1403 /label=-35 misc_feature 1404..1420 /label=lac O1 promoter 1421..1426 /label=-10 misc_feature 1379..1432 /label=BBa_R0011 promoter 1379..1432 /label=BBa_R0011 misc_feature 63..1433 /label=BBa_Q04121 misc_feature 1446..1449 /label=conserved RBS 1442..1453 /label=BBa_B0034 CDS 1460..2128 /label=cI 434 misc_feature 2090..2128 /label=LVA misc_feature 1460..2128 /label=BBa_C0052 misc_feature 2129..2153 /label=Help:Barcodes CDS 1460..2128 /label=BBa_C0052 stem_loop 2173..2216 /label=stem_loop misc_feature 2162..2241 /label=BBa_B0010 terminator 2162..2241 /label=BBa_B0010 stem_loop 2257..2276 /label=T7 TE misc_feature 2283..2283 /label=stop polyA_site 2277..2290 /label=polya misc_feature 2250..2290 /label=BBa_B0012 terminator 2250..2290 /label=BBa_B0012 terminator 2162..2290 /label=BBa_B0015 misc_feature 1..2290 /label=BBa_I12022 misc_feature 2299..2301 /label=OR3 misc_feature 2309..2326 /label=OR2 misc_feature 2332..2349 /label=OR1 promoter 2316..2321 /label=-35 promoter 2306..2311 /label=-35 promoter 2328..2333 /label=-10 misc_feature 2299..2352 /label=BBa_R0053 promoter 2299..2352 /label=BBa_R0053 misc_feature 2365..2368 /label=conserved RBS 2361..2372 /label=BBa_B0034 CDS 2382..2998 /label=tetR misc_feature 2999..3032 /label=SsrA misc_feature 3039..3063 /label=Help:Barcodes CDS 2379..3038 /label=BBa_C0040 stem_loop 3083..3126 /label=stem_loop misc_feature 3072..3151 /label=BBa_B0010 terminator 3072..3151 /label=BBa_B0010 stem_loop 3167..3186 /label=T7 TE misc_feature 3193..3193 /label=stop polyA_site 3187..3200 /label=polya misc_feature 3160..3200 /label=BBa_B0012 terminator 3160..3200 /label=BBa_B0012 misc_feature 3209..3227 /label=TetR 1 misc_feature 3209..3262 /label=BBa_R0040 promoter 3228..3233 /label=-35 misc_feature 3234..3252 /label=TetR 2 promoter 3251..3256 /label=-10 promoter 3209..3262 /label=BBa_R0040 misc_feature 3275..3278 /label=conserved RBS 3271..3282 /label=BBa_B0034 CDS 3292..3999 /label=cI lambda misc_feature 4000..4032 /label=LVA misc_feature 4039..4063 /label=Help:Barcodes CDS 3289..4038 /label=BBa_C0051 stem_loop 4083..4126 /label=stem_loop misc_feature 4072..4151 /label=BBa_B0010 terminator 4072..4151 /label=BBa_B0010 stem_loop 4167..4186 /label=T7 TE misc_feature 4193..4193 /label=stop polyA_site 4187..4200 /label=polya misc_feature 4160..4200 /label=BBa_B0012 terminator 4160..4200 /label=BBa_B0012 misc_feature 2299..4200 /label=BBa_I12023 misc_feature 4264..4277 /label=OR1 434 misc_feature 4217..4233 /label=OR1 lambda misc_feature 4241..4257 /label=OR2 lambda promoter 4279..4284 /label=-10 promoter 4256..4261 /label=-35 promoter 4209..4290 /label=BBa_I12006 misc_feature 4303..4306 /label=conserved RBS 4299..4310 /label=BBa_B0034 CDS 4317..5039 /label=BBa_E0030 stem_loop 5059..5102 /label=stem_loop misc_feature 5048..5127 /label=BBa_B0010 terminator 5048..5127 /label=BBa_B0010 stem_loop 5143..5162 /label=T7 TE misc_feature 5169..5169 /label=stop polyA_site 5163..5176 /label=polya misc_feature 5136..5176 /label=BBa_B0012 terminator 5136..5176 /label=BBa_B0012 misc_feature 4299..5176 /label=BBa_E0430 ORIGIN 1 aataaacttg actaaagatt cctttagtag ataatttaag tgttctttaa tttctactag 61 agaaagagga gaaatactag atggtgaatg tgaaaccagt aacgttatac gatgtcgcag 121 agtatgccgg tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc agccacgttt 181 ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac attcccaacc 241 gcgtggcaca acaactggcg ggcaaacagt cgttgctgat tggcgttgcc acctccagtc 301 tggccctgca cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc gatcaactgg 361 gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg 421 tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg ctggatgacc 481 aggatgccat tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt cttgatgtct 541 ctgaccagac acccatcaac agtattattt tctcccatga agacggtacg cgactgggcg 601 tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt 661 ctgtctcggc gcgtctgcgt ctggctggct ggcataaata tctcactcgc aatcaaattc 721 agccgatagc ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa caaaccatgc 781 aaatgctgaa tgagggcatc gttcccactg cgatgctggt tgccaacgat cagatggcgc 841 tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat atctcggtag 901 tgggatacga cgataccgaa gacagctcat gttatatccc gccgttaacc accatcaaac 961 aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc 1021 aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccctgg 1081 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac 1141 gacaggtttc ccgactggaa agcgggcagg ctgcaaacga cgaaaactac gctttagtag 1201 cttaataact ctgatagtgc tagtgtagat ctctactaga gccaggcatc aaataaaacg 1261 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct 1321 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata tactagagaa 1381 ttgtgagcgg ataacaattg acattgtgag cggataacaa gatactgagc acatactaga 1441 gaaagaggag aaatactaga tgagtatttc ttccagggta aaaagcaaaa gaatccagct 1501 tggacttaac caggctgaac ttgctcaaaa ggtggggact acccagcagt ctatagagca 1561 gctcgaaaac ggtaaaacta agcgaccacg ctttttacca gaacttgcgt cagctcttgg 1621 cgtaagtgtt gactggctgc tcaatggcac ctctgattcg aatgttagat ttgttgggca 1681 cgttgagccc aaagggaaat atccattgat tagcatggtt agagctggtt cgtggtgtga 1741 agcttgtgaa ccctacgata tcaaggacat tgatgaatgg tatgacagtg acgttaactt 1801 attaggcaat ggattctggc tgaaggttga aggtgattcc atgacctcac ctgtaggtca 1861 aagcatccct gaaggtcata tggtgttagt agatactgga cgggagccag tgaatggaag 1921 ccttgttgta gccaaactga ctgacgcgaa cgaagcaaca ttcaagaaac tggtcataga 1981 tggcggtcag aagtacctga aaggcctgaa tccttcatgg cctatgactc ctatcaacgg 2041 aaactgcaag attatcggtg ttgtcgtgga agcgagggta aaattcgtag ctgcaaacga 2101 cgaaaactac gctttagtag cttaataact ctgatagtgc tagtgtagat ctctactaga 2161 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt 2221 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc 2281 tgcgtttata tactagagaa taaacttgac taaagattcc tttagtagat aatttaagtg 2341 ttctttaatt tctactagag aaagaggaga aatactagat gtccagatta gataaaagta 2401 aagtgattaa cagcgcatta gagctgctta atgaggtcgg aatcgaaggt ttaacaaccc 2461 gtaaactcgc ccagaagcta ggtgtagagc agcctacatt gtattggcat gtaaaaaata 2521 agcgggcttt gctcgacgcc ttagccattg agatgttaga taggcaccat actcactttt 2581 gccctttaga aggggaaagc tggcaagatt ttttacgtaa taacgctaaa agttttagat 2641 gtgctttact aagtcatcgc gatggagcaa aagtacattt aggtacacgg cctacagaaa 2701 aacagtatga aactctcgaa aatcaattag cctttttatg ccaacaaggt ttttcactag 2761 agaatgcatt atatgcactc agcgctgtgg ggcattttac tttaggttgc gtattggaag 2821 atcaagagca tcaagtcgct aaagaagaaa gggaaacacc tactactgat agtatgccgc 2881 cattattacg acaagctatc gaattatttg atcaccaagg tgcagagcca gccttcttat 2941 tcggccttga attgatcata tgcggattag aaaaacaact taaatgtgaa agtgggtccg 3001 ctgcaaacga cgaaaactac gctttagtag cttaataaca ctgatagtgc tagtgtagat 3061 cactactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 3121 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg 3181 tgggcctttc tgcgtttata tactagagtc cctatcagtg atagagattg acatccctat 3241 cagtgataga gatactgagc actactagag aaagaggaga aatactagat gagcacaaaa 3301 aagaaaccat taacacaaga gcagcttgag gacgcacgtc gccttaaagc aatttatgaa 3361 aaaaagaaaa atgaacttgg cttatcccag gaatctgtcg cagacaagat ggggatgggg 3421 cagtcaggcg ttggtgcttt atttaatggc atcaatgcat taaatgctta taacgccgca 3481 ttgcttgcaa aaattctcaa agttagcgtt gaagaattta gcccttcaat cgccagagaa 3541 atctacgaga tgtatgaagc ggttagtatg cagccgtcac ttagaagtga gtatgagtac 3601 cctgtttttt ctcatgttca ggcagggatg ttctcacctg agcttagaac ctttaccaaa 3661 ggtgatgcgg agagatgggt aagcacaacc aaaaaagcca gtgattctgc attctggctt 3721 gaggttgaag gtaattccat gaccgcacca acaggctcca agccaagctt tcctgacgga 3781 atgttaattc tcgttgaccc tgagcaggct gttgagccag gtgatttctg catagccaga 3841 cttgggggtg atgagtttac cttcaagaaa ctgatcaggg atagcggtca ggtgttttta 3901 caaccactaa acccacagta cccaatgatc ccatgcaatg agagttgttc cgttgtgggg 3961 aaagttatcg ctagtcagtg gcctgaagag acgtttggcg ctgcaaacga cgaaaactac 4021 gctttagtag cttaataacg ctgatagtgc tagtgtagat cgctactaga gccaggcatc 4081 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg 4141 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata 4201 tactagaggc aaccattatc accgccagag gtaaaatagt caacacgcac ggtgttagat 4261 attacaaact ttcttgtata gatttaacgt tactagagaa agaggagaaa tactagatgg 4321 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg 4381 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca 4441 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg 4501 tgaccacctt cggctacggc ctgcaatgct tcgcccgcta ccccgaccac atgaagctgc 4561 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca 4621 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga 4681 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc 4741 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca 4801 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc 4861 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc 4921 tgagctacca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc 4981 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaataat 5041 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt 5101 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg 5161 cctttctgcg tttata //