LOCUS       BBa_I12022              2290 bp    DNA     linear       13-May-2024
DEFINITION  Inducible 434 cI Repressor Generator (Controlled by IPTG and LacI)
ACCESSION   BBa_I12022
VERSION     BBa_I12022.1  
FEATURES             Location/Qualifiers
     misc_feature    1..3
                     /label=OR3
     misc_feature    11..28
                     /label=OR2
     misc_feature    34..51
                     /label=OR1
     promoter        18..23
                     /label=-35
     promoter        8..13
                     /label=-35
     promoter        30..35
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0053
     promoter        1..54
                     /label=BBa_R0053
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    1170..1208
                     /label=LVA
     CDS             81..1208
                     /label=lacI-LVA
     misc_feature    81..1208
                     /label=BBa_C0012
     misc_feature    1209..1233
                     /label=Help:Barcodes
     CDS             81..1208
                     /label=BBa_C0012
     stem_loop       1253..1296
                     /label=stem_loop
     misc_feature    1242..1321
                     /label=BBa_B0010
     terminator      1242..1321
                     /label=BBa_B0010
     stem_loop       1337..1356
                     /label=T7 TE
     misc_feature    1363..1363
                     /label=stop
     polyA_site      1357..1370
                     /label=polya
     misc_feature    1330..1370
                     /label=BBa_B0012
     terminator      1330..1370
                     /label=BBa_B0012
     terminator      1242..1370
                     /label=BBa_B0015
     misc_feature    1381..1397
                     /label=lac O1
     promoter        1398..1403
                     /label=-35
     misc_feature    1404..1420
                     /label=lac O1
     promoter        1421..1426
                     /label=-10
     misc_feature    1379..1432
                     /label=BBa_R0011
     promoter        1379..1432
                     /label=BBa_R0011
     misc_feature    63..1433
                     /label=BBa_Q04121
     misc_feature    1446..1449
                     /label=conserved
     RBS             1442..1453
                     /label=BBa_B0034
     CDS             1460..2128
                     /label=cI 434
     misc_feature    2090..2128
                     /label=LVA
     misc_feature    1460..2128
                     /label=BBa_C0052
     misc_feature    2129..2153
                     /label=Help:Barcodes
     CDS             1460..2128
                     /label=BBa_C0052
     stem_loop       2173..2216
                     /label=stem_loop
     misc_feature    2162..2241
                     /label=BBa_B0010
     terminator      2162..2241
                     /label=BBa_B0010
     stem_loop       2257..2276
                     /label=T7 TE
     misc_feature    2283..2283
                     /label=stop
     polyA_site      2277..2290
                     /label=polya
     misc_feature    2250..2290
                     /label=BBa_B0012
     terminator      2250..2290
                     /label=BBa_B0012
     terminator      2162..2290
                     /label=BBa_B0015
ORIGIN
        1 aataaacttg actaaagatt cctttagtag ataatttaag tgttctttaa tttctactag
       61 agaaagagga gaaatactag atggtgaatg tgaaaccagt aacgttatac gatgtcgcag
      121 agtatgccgg tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc agccacgttt
      181 ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac attcccaacc
      241 gcgtggcaca acaactggcg ggcaaacagt cgttgctgat tggcgttgcc acctccagtc
      301 tggccctgca cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc gatcaactgg
      361 gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg
      421 tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg ctggatgacc
      481 aggatgccat tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt cttgatgtct
      541 ctgaccagac acccatcaac agtattattt tctcccatga agacggtacg cgactgggcg
      601 tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt
      661 ctgtctcggc gcgtctgcgt ctggctggct ggcataaata tctcactcgc aatcaaattc
      721 agccgatagc ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa caaaccatgc
      781 aaatgctgaa tgagggcatc gttcccactg cgatgctggt tgccaacgat cagatggcgc
      841 tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat atctcggtag
      901 tgggatacga cgataccgaa gacagctcat gttatatccc gccgttaacc accatcaaac
      961 aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc
     1021 aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccctgg
     1081 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     1141 gacaggtttc ccgactggaa agcgggcagg ctgcaaacga cgaaaactac gctttagtag
     1201 cttaataact ctgatagtgc tagtgtagat ctctactaga gccaggcatc aaataaaacg
     1261 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     1321 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata tactagagaa
     1381 ttgtgagcgg ataacaattg acattgtgag cggataacaa gatactgagc acatactaga
     1441 gaaagaggag aaatactaga tgagtatttc ttccagggta aaaagcaaaa gaatccagct
     1501 tggacttaac caggctgaac ttgctcaaaa ggtggggact acccagcagt ctatagagca
     1561 gctcgaaaac ggtaaaacta agcgaccacg ctttttacca gaacttgcgt cagctcttgg
     1621 cgtaagtgtt gactggctgc tcaatggcac ctctgattcg aatgttagat ttgttgggca
     1681 cgttgagccc aaagggaaat atccattgat tagcatggtt agagctggtt cgtggtgtga
     1741 agcttgtgaa ccctacgata tcaaggacat tgatgaatgg tatgacagtg acgttaactt
     1801 attaggcaat ggattctggc tgaaggttga aggtgattcc atgacctcac ctgtaggtca
     1861 aagcatccct gaaggtcata tggtgttagt agatactgga cgggagccag tgaatggaag
     1921 ccttgttgta gccaaactga ctgacgcgaa cgaagcaaca ttcaagaaac tggtcataga
     1981 tggcggtcag aagtacctga aaggcctgaa tccttcatgg cctatgactc ctatcaacgg
     2041 aaactgcaag attatcggtg ttgtcgtgga agcgagggta aaattcgtag ctgcaaacga
     2101 cgaaaactac gctttagtag cttaataact ctgatagtgc tagtgtagat ctctactaga
     2161 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
     2221 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc
     2281 tgcgtttata
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