LOCUS       BBa_I12023              1902 bp    DNA     linear       10-May-2024
DEFINITION  Inducible Lambda cI Repressor Generator (Controlled by ATC and 
            TetR)
ACCESSION   BBa_I12023
VERSION     BBa_I12023.1  
FEATURES             Location/Qualifiers
     misc_feature    1..3
                     /label=OR3
     misc_feature    11..28
                     /label=OR2
     misc_feature    34..51
                     /label=OR1
     promoter        18..23
                     /label=-35
     promoter        8..13
                     /label=-35
     promoter        30..35
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0053
     promoter        1..54
                     /label=BBa_R0053
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             84..700
                     /label=tetR
     misc_feature    701..734
                     /label=SsrA
     misc_feature    741..765
                     /label=Help:Barcodes
     CDS             81..740
                     /label=BBa_C0040
     stem_loop       785..828
                     /label=stem_loop
     misc_feature    774..853
                     /label=BBa_B0010
     terminator      774..853
                     /label=BBa_B0010
     stem_loop       869..888
                     /label=T7 TE
     misc_feature    895..895
                     /label=stop
     polyA_site      889..902
                     /label=polya
     misc_feature    862..902
                     /label=BBa_B0012
     terminator      862..902
                     /label=BBa_B0012
     misc_feature    911..929
                     /label=TetR 1
     misc_feature    911..964
                     /label=BBa_R0040
     promoter        930..935
                     /label=-35
     misc_feature    936..954
                     /label=TetR 2
     promoter        953..958
                     /label=-10
     promoter        911..964
                     /label=BBa_R0040
     misc_feature    977..980
                     /label=conserved
     RBS             973..984
                     /label=BBa_B0034
     CDS             994..1701
                     /label=cI lambda
     misc_feature    1702..1734
                     /label=LVA
     misc_feature    1741..1765
                     /label=Help:Barcodes
     CDS             991..1740
                     /label=BBa_C0051
     stem_loop       1785..1828
                     /label=stem_loop
     misc_feature    1774..1853
                     /label=BBa_B0010
     terminator      1774..1853
                     /label=BBa_B0010
     stem_loop       1869..1888
                     /label=T7 TE
     misc_feature    1895..1895
                     /label=stop
     polyA_site      1889..1902
                     /label=polya
     misc_feature    1862..1902
                     /label=BBa_B0012
     terminator      1862..1902
                     /label=BBa_B0012
ORIGIN
        1 aataaacttg actaaagatt cctttagtag ataatttaag tgttctttaa tttctactag
       61 agaaagagga gaaatactag atgtccagat tagataaaag taaagtgatt aacagcgcat
      121 tagagctgct taatgaggtc ggaatcgaag gtttaacaac ccgtaaactc gcccagaagc
      181 taggtgtaga gcagcctaca ttgtattggc atgtaaaaaa taagcgggct ttgctcgacg
      241 ccttagccat tgagatgtta gataggcacc atactcactt ttgcccttta gaaggggaaa
      301 gctggcaaga ttttttacgt aataacgcta aaagttttag atgtgcttta ctaagtcatc
      361 gcgatggagc aaaagtacat ttaggtacac ggcctacaga aaaacagtat gaaactctcg
      421 aaaatcaatt agccttttta tgccaacaag gtttttcact agagaatgca ttatatgcac
      481 tcagcgctgt ggggcatttt actttaggtt gcgtattgga agatcaagag catcaagtcg
      541 ctaaagaaga aagggaaaca cctactactg atagtatgcc gccattatta cgacaagcta
      601 tcgaattatt tgatcaccaa ggtgcagagc cagccttctt attcggcctt gaattgatca
      661 tatgcggatt agaaaaacaa cttaaatgtg aaagtgggtc cgctgcaaac gacgaaaact
      721 acgctttagt agcttaataa cactgatagt gctagtgtag atcactacta gagccaggca
      781 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
      841 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta
      901 tatactagag tccctatcag tgatagagat tgacatccct atcagtgata gagatactga
      961 gcactactag agaaagagga gaaatactag atgagcacaa aaaagaaacc attaacacaa
     1021 gagcagcttg aggacgcacg tcgccttaaa gcaatttatg aaaaaaagaa aaatgaactt
     1081 ggcttatccc aggaatctgt cgcagacaag atggggatgg ggcagtcagg cgttggtgct
     1141 ttatttaatg gcatcaatgc attaaatgct tataacgccg cattgcttgc aaaaattctc
     1201 aaagttagcg ttgaagaatt tagcccttca atcgccagag aaatctacga gatgtatgaa
     1261 gcggttagta tgcagccgtc acttagaagt gagtatgagt accctgtttt ttctcatgtt
     1321 caggcaggga tgttctcacc tgagcttaga acctttacca aaggtgatgc ggagagatgg
     1381 gtaagcacaa ccaaaaaagc cagtgattct gcattctggc ttgaggttga aggtaattcc
     1441 atgaccgcac caacaggctc caagccaagc tttcctgacg gaatgttaat tctcgttgac
     1501 cctgagcagg ctgttgagcc aggtgatttc tgcatagcca gacttggggg tgatgagttt
     1561 accttcaaga aactgatcag ggatagcggt caggtgtttt tacaaccact aaacccacag
     1621 tacccaatga tcccatgcaa tgagagttgt tccgttgtgg ggaaagttat cgctagtcag
     1681 tggcctgaag agacgtttgg cgctgcaaac gacgaaaact acgctttagt agcttaataa
     1741 cgctgatagt gctagtgtag atcgctacta gagccaggca tcaaataaaa cgaaaggctc
     1801 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga
     1861 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta
//