LOCUS       BBa_I12025              1969 bp    DNA     linear       10-May-2024
DEFINITION  Test construct for I12007
ACCESSION   BBa_I12025
VERSION     BBa_I12025.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     CDS             85..792
                     /label=cI lambda
     misc_feature    793..825
                     /label=LVA
     misc_feature    832..856
                     /label=Help:Barcodes
     CDS             82..856
                     /label=BBa_C0051
     stem_loop       876..919
                     /label=stem_loop
     misc_feature    865..944
                     /label=BBa_B0010
     terminator      865..944
                     /label=BBa_B0010
     stem_loop       960..979
                     /label=T7 TE
     misc_feature    986..986
                     /label=stop
     polyA_site      980..993
                     /label=polya
     misc_feature    953..993
                     /label=BBa_B0012
     terminator      953..993
                     /label=BBa_B0012
     terminator      865..993
                     /label=BBa_B0015
     misc_feature    1..993
                     /label=BBa_I12024
     misc_feature    1010..1026
                     /label=OR1
     misc_feature    1034..1050
                     /label=OR2
     promoter        1049..1054
                     /label=-35
     misc_feature    1060..1066
                     /label=mutate to obliterate OR3
     promoter        1072..1077
                     /label=-10
     promoter        1002..1083
                     /label=BBa_I12007
     misc_feature    1096..1099
                     /label=conserved
     RBS             1092..1103
                     /label=BBa_B0034
     CDS             1110..1832
                     /label=BBa_E0030
     stem_loop       1852..1895
                     /label=stem_loop
     misc_feature    1841..1920
                     /label=BBa_B0010
     terminator      1841..1920
                     /label=BBa_B0010
     stem_loop       1936..1955
                     /label=T7 TE
     misc_feature    1962..1962
                     /label=stop
     polyA_site      1956..1969
                     /label=polya
     misc_feature    1929..1969
                     /label=BBa_B0012
     terminator      1929..1969
                     /label=BBa_B0012
     misc_feature    1092..1969
                     /label=BBa_E0430
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgagcaca aaaaagaaac cattaacaca agagcagctt
      121 gaggacgcac gtcgccttaa agcaatttat gaaaaaaaga aaaatgaact tggcttatcc
      181 caggaatctg tcgcagacaa gatggggatg gggcagtcag gcgttggtgc tttatttaat
      241 ggcatcaatg cattaaatgc ttataacgcc gcattgcttg caaaaattct caaagttagc
      301 gttgaagaat ttagcccttc aatcgccaga gaaatctacg agatgtatga agcggttagt
      361 atgcagccgt cacttagaag tgagtatgag taccctgttt tttctcatgt tcaggcaggg
      421 atgttctcac ctgagcttag aacctttacc aaaggtgatg cggagagatg ggtaagcaca
      481 accaaaaaag ccagtgattc tgcattctgg cttgaggttg aaggtaattc catgaccgca
      541 ccaacaggct ccaagccaag ctttcctgac ggaatgttaa ttctcgttga ccctgagcag
      601 gctgttgagc caggtgattt ctgcatagcc agacttgggg gtgatgagtt taccttcaag
      661 aaactgatca gggatagcgg tcaggtgttt ttacaaccac taaacccaca gtacccaatg
      721 atcccatgca atgagagttg ttccgttgtg gggaaagtta tcgctagtca gtggcctgaa
      781 gagacgtttg gcgctgcaaa cgacgaaaac tacgctttag tagcttaata acgctgatag
      841 tgctagtgta gatcgctact agagccaggc atcaaataaa acgaaaggct cagtcgaaag
      901 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
      961 gctcaccttc gggtgggcct ttctgcgttt atatactaga ggcaaccatt atcaccgcca
     1021 gaggtaaaat agtcaacacg cacggtgtta gatatttata aatagtggtg atagatttaa
     1081 cgttactaga gaaagaggag aaatactaga tggtgagcaa gggcgaggag ctgttcaccg
     1141 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
     1201 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
     1261 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cttcggctac ggcctgcaat
     1321 gcttcgcccg ctaccccgac cacatgaagc tgcacgactt cttcaagtcc gccatgcccg
     1381 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     1441 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     1501 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
     1561 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
     1621 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     1681 acggccccgt gctgctgccc gacaaccact acctgagcta ccagtccgcc ctgagcaaag
     1741 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     1801 ctctcggcat ggacgagctg tacaagtaat aatactagag ccaggcatca aataaaacga
     1861 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     1921 tactagagtc acactggctc accttcgggt gggcctttct gcgtttata
//