##gff-version 3
##sequence-region BBa_I12028 1 4044
BBa_I12028	.	engineered_region	1002	3068	.	+	0	ID=BBa_I12027;Name=BBa_I12027
BBa_I12028	.	terminator	3028	3068	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I12027
BBa_I12028	.	stop_codon	3061	3061	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I12028	.	polyA_site	3055	3068	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I12028	.	stem_loop	3035	3054	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I12028	.	engineered_region	3028	3068	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I12028	.	ribosome_entry_site	2220	2231	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I12027
BBa_I12028	.	conserved_region	2224	2227	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I12028	.	CDS	2238	2906	.	+	0	ID=BBa_C0052;Name=BBa_C0052;Parent=BBa_I12027
BBa_I12028	.	engineered_region	2238	2906	.	+	0	ID=annotation7035;Name=BBa_C0052;Parent=BBa_C0052
BBa_I12028	.	CDS	2238	2906	.	+	0	ID=annotation1743;Name=cI 434;Parent=BBa_C0052
BBa_I12028	.	engineered_tag	2907	2931	.	+	0	ID=annotation2213992;Name=Help:Barcodes;Parent=BBa_C0052
BBa_I12028	.	tag	2868	2906	.	+	0	ID=annotation1745;Name=LVA;Parent=BBa_C0052
BBa_I12028	.	terminator	2940	3019	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I12027
BBa_I12028	.	stem_loop	2951	2994	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I12028	.	engineered_region	2940	3019	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I12028	.	promoter	1002	2211	.	+	0	ID=BBa_I0500;Name=BBa_I0500;Parent=BBa_I12027
BBa_I12028	.	promoter	2081	2211	.	+	0	ID=annotation1498841;Name=Pbad;Parent=BBa_I0500
BBa_I12028	.	promoter	2044	2115	.	+	0	ID=annotation1498842;Name=PC;Parent=BBa_I0500
BBa_I12028	.	sequence_feature	1002	2080	.	+	0	ID=annotation1498843;Name=AraC;Parent=BBa_I0500
BBa_I12028	.	engineered_region	1	993	.	+	0	ID=BBa_I12024;Name=BBa_I12024
BBa_I12028	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_I12024
BBa_I12028	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_I12028	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_I12028	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_I12028	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_I12028	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_I12028	.	CDS	82	856	.	+	0	ID=BBa_C0051;Name=BBa_C0051;Parent=BBa_I12024
BBa_I12028	.	engineered_tag	832	856	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_I12028	.	CDS	85	792	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_I12028	.	tag	793	825	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_I12028	.	terminator	865	993	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_I12024
BBa_I12028	.	terminator	953	993	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I12028	.	stop_codon	986	986	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I12028	.	polyA_site	980	993	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I12028	.	stem_loop	960	979	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I12028	.	engineered_region	953	993	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I12028	.	terminator	865	944	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I12028	.	stem_loop	876	919	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I12028	.	engineered_region	865	944	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I12028	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I12024
BBa_I12028	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I12028	.	engineered_region	3167	4044	.	+	0	ID=BBa_E0430;Name=BBa_E0430
BBa_I12028	.	terminator	4004	4044	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0430
BBa_I12028	.	stop_codon	4037	4037	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I12028	.	polyA_site	4031	4044	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I12028	.	stem_loop	4011	4030	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I12028	.	engineered_region	4004	4044	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I12028	.	ribosome_entry_site	3167	3178	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0430
BBa_I12028	.	conserved_region	3171	3174	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I12028	.	terminator	3916	3995	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0430
BBa_I12028	.	stem_loop	3927	3970	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I12028	.	engineered_region	3916	3995	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I12028	.	CDS	3185	3907	.	+	0	ID=BBa_E0030;Name=BBa_E0030;Parent=BBa_E0430
BBa_I12028	.	promoter	3077	3158	.	+	0	ID=BBa_I12006;Name=BBa_I12006
BBa_I12028	.	non_covalent_binding_site	3085	3101	.	+	0	ID=annotation786500;Name=OR1 lambda;Parent=BBa_I12006
BBa_I12028	.	promoter	3147	3152	.	+	0	ID=annotation837228;Name=-10;Parent=BBa_I12006
BBa_I12028	.	non_covalent_binding_site	3109	3125	.	+	0	ID=annotation786518;Name=OR2 lambda;Parent=BBa_I12006
BBa_I12028	.	promoter	3124	3129	.	+	0	ID=annotation837284;Name=-35;Parent=BBa_I12006
BBa_I12028	.	non_covalent_binding_site	3132	3145	.	+	0	ID=annotation786365;Name=OR1 434;Parent=BBa_I12006
>BBa_I12028
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatact
agatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaa
gaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttattt
aatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcc
cttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccc
tgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggta
agcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctcca
agccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccag
acttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacag
tacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttg
gcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccagg
catcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctact
agagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttatgacaacttgacggctacatcat
tcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagag
ttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctg
atacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgaca
agcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcg
tacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagattt
atcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggct
ggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcg
cggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcggg
aacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagat
tgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacc
cgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccg
ccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaa
ccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaag
tgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatcc
ataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagct
actagagaaagaggagaaatactagatgagtatttcttccagggtaaaaagcaaaagaatccagcttggacttaaccag
gctgaacttgctcaaaaggtggggactacccagcagtctatagagcagctcgaaaacggtaaaactaagcgaccacgct
ttttaccagaacttgcgtcagctcttggcgtaagtgttgactggctgctcaatggcacctctgattcgaatgttagatt
tgttgggcacgttgagcccaaagggaaatatccattgattagcatggttagagctggttcgtggtgtgaagcttgtgaa
ccctacgatatcaaggacattgatgaatggtatgacagtgacgttaacttattaggcaatggattctggctgaaggttg
aaggtgattccatgacctcacctgtaggtcaaagcatccctgaaggtcatatggtgttagtagatactggacgggagcc
agtgaatggaagccttgttgtagccaaactgactgacgcgaacgaagcaacattcaagaaactggtcatagatggcggt
cagaagtacctgaaaggcctgaatccttcatggcctatgactcctatcaacggaaactgcaagattatcggtgttgtcg
tggaagcgagggtaaaattcgtagctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtg
tagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtt
tgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggcaac
cattatcaccgccagaggtaaaatagtcaacacgcacggtgttagatattacaaactttcttgtatagatttaacgtta
ctagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagc
tggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccct
gaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgc
ttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgca
ccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcat
cgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaac
gtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcg
tgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgag
ctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccggg
atcactctcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaa
gactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggc
ctttctgcgtttata