LOCUS       BBa_I12031              1157 bp    DNA     linear       10-May-2024
DEFINITION  Barkai-Leibler design experiment Part A (Lambda cI) wth 
            cooperativity
ACCESSION   BBa_I12031
VERSION     BBa_I12031.1  
FEATURES             Location/Qualifiers
     misc_feature    1..41
                     /label=B0012
     misc_feature    50..129
                     /label=B0010
     terminator      1..129
                     /label=BBa_B0025
     misc_feature    146..162
                     /label=OR1 lambda
     promoter        185..190
                     /label=-35
     misc_feature    170..186
                     /label=OR2 lambda
     misc_feature    193..206
                     /label=OR1 434
     promoter        208..213
                     /label=-10
     misc_feature    214..228
                     /label=OR2 434
     RBS             229..240
                     /label=BBa_B0034 (Elowitz)
     misc_feature    233..238
                     /label=conserved
     promoter        138..239
                     /label=BBa_I12034
     CDS             249..956
                     /label=cI lambda
     misc_feature    957..989
                     /label=LVA
     misc_feature    996..1020
                     /label=Help:Barcodes
     CDS             246..1020
                     /label=BBa_C0051
     stem_loop       1040..1083
                     /label=stem_loop
     misc_feature    1029..1108
                     /label=BBa_B0010
     terminator      1029..1108
                     /label=BBa_B0010
     stem_loop       1124..1143
                     /label=T7 TE
     misc_feature    1150..1150
                     /label=stop
     polyA_site      1144..1157
                     /label=polya
     misc_feature    1117..1157
                     /label=BBa_B0012
     terminator      1117..1157
                     /label=BBa_B0012
     terminator      1029..1157
                     /label=BBa_B0015
ORIGIN
        1 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca
       61 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt
      121 gatgcctggt actagaggca accattatca ccgccagagg taaaatagtc aacacgcacg
      181 gtgttagata ttacaaactt tcttgtatag attacaatgt atcttgtaaa gaggagaaat
      241 actagatgag cacaaaaaag aaaccattaa cacaagagca gcttgaggac gcacgtcgcc
      301 ttaaagcaat ttatgaaaaa aagaaaaatg aacttggctt atcccaggaa tctgtcgcag
      361 acaagatggg gatggggcag tcaggcgttg gtgctttatt taatggcatc aatgcattaa
      421 atgcttataa cgccgcattg cttgcaaaaa ttctcaaagt tagcgttgaa gaatttagcc
      481 cttcaatcgc cagagaaatc tacgagatgt atgaagcggt tagtatgcag ccgtcactta
      541 gaagtgagta tgagtaccct gttttttctc atgttcaggc agggatgttc tcacctgagc
      601 ttagaacctt taccaaaggt gatgcggaga gatgggtaag cacaaccaaa aaagccagtg
      661 attctgcatt ctggcttgag gttgaaggta attccatgac cgcaccaaca ggctccaagc
      721 caagctttcc tgacggaatg ttaattctcg ttgaccctga gcaggctgtt gagccaggtg
      781 atttctgcat agccagactt gggggtgatg agtttacctt caagaaactg atcagggata
      841 gcggtcaggt gtttttacaa ccactaaacc cacagtaccc aatgatccca tgcaatgaga
      901 gttgttccgt tgtggggaaa gttatcgcta gtcagtggcc tgaagagacg tttggcgctg
      961 caaacgacga aaactacgct ttagtagctt aataacgctg atagtgctag tgtagatcgc
     1021 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
     1081 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg
     1141 gcctttctgc gtttata
//