LOCUS BBa_I12039 4053 bp DNA linear 25-Nov-2024 DEFINITION Test construct for BBa_I12036 ACCESSION BBa_I12039 VERSION BBa_I12039.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 CDS 85..792 /label=cI lambda misc_feature 793..825 /label=LVA misc_feature 832..856 /label=Help:Barcodes CDS 82..856 /label=BBa_C0051 stem_loop 876..919 /label=stem_loop misc_feature 865..944 /label=BBa_B0010 terminator 865..944 /label=BBa_B0010 stem_loop 960..979 /label=T7 TE misc_feature 986..986 /label=stop polyA_site 980..993 /label=polya misc_feature 953..993 /label=BBa_B0012 terminator 953..993 /label=BBa_B0012 terminator 865..993 /label=BBa_B0015 misc_feature 1..993 /label=BBa_I12024 promoter 2081..2211 /label=Pbad promoter 2044..2115 /label=PC misc_feature 1002..2080 /label=AraC promoter 1002..2211 /label=BBa_I0500 misc_feature 2224..2227 /label=conserved RBS 2220..2231 /label=BBa_B0034 CDS 2238..2906 /label=cI 434 misc_feature 2868..2906 /label=LVA misc_feature 2238..2906 /label=BBa_C0052 misc_feature 2907..2931 /label=Help:Barcodes CDS 2238..2906 /label=BBa_C0052 stem_loop 2951..2994 /label=stem_loop misc_feature 2940..3019 /label=BBa_B0010 terminator 2940..3019 /label=BBa_B0010 stem_loop 3035..3054 /label=T7 TE misc_feature 3061..3061 /label=stop polyA_site 3055..3068 /label=polya misc_feature 3028..3068 /label=BBa_B0012 terminator 3028..3068 /label=BBa_B0012 misc_feature 1002..3068 /label=BBa_I12027 misc_feature 3085..3101 /label=OR1 lambda misc_feature 3109..3125 /label=OR2 lambda misc_feature 3132..3145 /label=OR1 434 misc_feature 3154..3167 /label=OR2 434 promoter 3124..3129 /label=-35 promoter 3147..3152 /label=-10 misc_feature 3159..3159 /label=start promoter 3077..3167 /label=BBa_I12036 misc_feature 3180..3183 /label=conserved RBS 3176..3187 /label=BBa_B0034 CDS 3194..3916 /label=BBa_E0030 stem_loop 3936..3979 /label=stem_loop misc_feature 3925..4004 /label=BBa_B0010 terminator 3925..4004 /label=BBa_B0010 stem_loop 4020..4039 /label=T7 TE misc_feature 4046..4046 /label=stop polyA_site 4040..4053 /label=polya misc_feature 4013..4053 /label=BBa_B0012 terminator 4013..4053 /label=BBa_B0012 misc_feature 3176..4053 /label=BBa_E0430 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagaaagagg agaaatacta gatgagcaca aaaaagaaac cattaacaca agagcagctt 121 gaggacgcac gtcgccttaa agcaatttat gaaaaaaaga aaaatgaact tggcttatcc 181 caggaatctg tcgcagacaa gatggggatg gggcagtcag gcgttggtgc tttatttaat 241 ggcatcaatg cattaaatgc ttataacgcc gcattgcttg caaaaattct caaagttagc 301 gttgaagaat ttagcccttc aatcgccaga gaaatctacg agatgtatga agcggttagt 361 atgcagccgt cacttagaag tgagtatgag taccctgttt tttctcatgt tcaggcaggg 421 atgttctcac ctgagcttag aacctttacc aaaggtgatg cggagagatg ggtaagcaca 481 accaaaaaag ccagtgattc tgcattctgg cttgaggttg aaggtaattc catgaccgca 541 ccaacaggct ccaagccaag ctttcctgac ggaatgttaa ttctcgttga ccctgagcag 601 gctgttgagc caggtgattt ctgcatagcc agacttgggg gtgatgagtt taccttcaag 661 aaactgatca gggatagcgg tcaggtgttt ttacaaccac taaacccaca gtacccaatg 721 atcccatgca atgagagttg ttccgttgtg gggaaagtta tcgctagtca gtggcctgaa 781 gagacgtttg gcgctgcaaa cgacgaaaac tacgctttag tagcttaata acgctgatag 841 tgctagtgta gatcgctact agagccaggc atcaaataaa acgaaaggct cagtcgaaag 901 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg 961 gctcaccttc gggtgggcct ttctgcgttt atatactaga gttatgacaa cttgacggct 1021 acatcattca ctttttcttc acaaccggca cggaactcgc tcgggctggc cccggtgcat 1081 tttttaaata cccgcgagaa atagagttga tcgtcaaaac caacattgcg accgacggtg 1141 gcgataggca tccgggtggt gctcaaaagc agcttcgcct ggctgatacg ttggtcctcg 1201 cgccagctta agacgctaat ccctaactgc tggcggaaaa gatgtgacag acgcgacggc 1261 gacaagcaaa catgctgtgc gacgctggcg atatcaaaat tgctgtctgc caggtgatcg 1321 ctgatgtact gacaagcctc gcgtacccga ttatccatcg gtggatggag cgactcgtta 1381 atcgcttcca tgcgccgcag taacaattgc tcaagcagat ttatcgccag cagctccgaa 1441 tagcgccctt ccccttgccc ggcgttaatg atttgcccaa acaggtcgct gaaatgcggc 1501 tggtgcgctt catccgggcg aaagaacccc gtattggcaa atattgacgg ccagttaagc 1561 cattcatgcc agtaggcgcg cggacgaaag taaacccact ggtgatacca ttcgcgagcc 1621 tccggatgac gaccgtagtg atgaatctct cctggcggga acagcaaaat atcacccggt 1681 cggcaaacaa attctcgtcc ctgatttttc accaccccct gaccgcgaat ggtgagattg 1741 agaatataac ctttcattcc cagcggtcgg tcgataaaaa aatcgagata accgttggcc 1801 tcaatcggcg ttaaacccgc caccagatgg gcattaaacg agtatcccgg cagcagggga 1861 tcattttgcg cttcagccat acttttcata ctcccgccat tcagagaaga aaccaattgt 1921 ccatattgca tcagacattg ccgtcactgc gtcttttact ggctcttctc gctaaccaaa 1981 ccggtaaccc cgcttattaa aagcattctg taacaaagcg ggaccaaagc catgacaaaa 2041 acgcgtaaca aaagtgtcta taatcacggc agaaaagtcc acattgatta tttgcacggc 2101 gtcacacttt gctatgccat agcattttta tccataagat tagcggatcc tacctgacgc 2161 tttttatcgc aactctctac tgtttctcca tacccgtttt tttgggctag ctactagaga 2221 aagaggagaa atactagatg agtatttctt ccagggtaaa aagcaaaaga atccagcttg 2281 gacttaacca ggctgaactt gctcaaaagg tggggactac ccagcagtct atagagcagc 2341 tcgaaaacgg taaaactaag cgaccacgct ttttaccaga acttgcgtca gctcttggcg 2401 taagtgttga ctggctgctc aatggcacct ctgattcgaa tgttagattt gttgggcacg 2461 ttgagcccaa agggaaatat ccattgatta gcatggttag agctggttcg tggtgtgaag 2521 cttgtgaacc ctacgatatc aaggacattg atgaatggta tgacagtgac gttaacttat 2581 taggcaatgg attctggctg aaggttgaag gtgattccat gacctcacct gtaggtcaaa 2641 gcatccctga aggtcatatg gtgttagtag atactggacg ggagccagtg aatggaagcc 2701 ttgttgtagc caaactgact gacgcgaacg aagcaacatt caagaaactg gtcatagatg 2761 gcggtcagaa gtacctgaaa ggcctgaatc cttcatggcc tatgactcct atcaacggaa 2821 actgcaagat tatcggtgtt gtcgtggaag cgagggtaaa attcgtagct gcaaacgacg 2881 aaaactacgc tttagtagct taataactct gatagtgcta gtgtagatct ctactagagc 2941 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg 3001 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg 3061 cgtttatata ctagaggcaa ccattatcac cgccagaggt aaaatagtca acacgcacgg 3121 tgttagatat tacaaacttt cttgtataga tttacaatgt atcttgttac tagagaaaga 3181 ggagaaatac tagatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct 3241 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg 3301 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt 3361 gccctggccc accctcgtga ccaccttcgg ctacggcctg caatgcttcg cccgctaccc 3421 cgaccacatg aagctgcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga 3481 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga 3541 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa 3601 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga 3661 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag 3721 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct 3781 gcccgacaac cactacctga gctaccagtc cgccctgagc aaagacccca acgagaagcg 3841 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga 3901 gctgtacaag taataatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag 3961 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg 4021 gctcaccttc gggtgggcct ttctgcgttt ata //