BBa_I12042
type: ComponentDefinition
persistent identity: BBa I12042
display id: BBa_I12042
version: 1
was generated by: iGEM to SBOL conversion
description: BL oscillator, no cooperativity, reporter protein, kickstart
created: 2004-08-19T11:00:00Z
modified: 2015-08-31T04:07:31Z
owned by: james
owned by: myers
mutable description: This part is a Barkai-Leibler oscillator with no cooperativity for the repressor (one 434 cI OR site). A reporter protein (EYFP) and a kickstart is included.
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 103
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: There is no cooperativity for 434 cI in this oscillator. A protein EYFP (with degradation tag) coding region is placed directly after the lambda cI coding region in order to keep track of the lambda cI levels. This part also includes a "kickstart" that will yield lambda cI if ATC is added.
star: false
creator: Hans
type: DnaRegion
role: partType/Project
role: SO:0000804
component: BBa_I12043
component: BBa_I12038
component: BBa_I12002
sequence annotation: BBa_I12038
sequence annotation: BBa_I12043
sequence annotation: BBa_I12002
sequence: https://synbiohub.org/public/igem/BBa_I12042_sequence/1

BBa_I12043
type: Component
persistent identity: component2238925
display id: component2238925
version: 1
top level: BBa_I12042
definition: BBa_I12043
access: public

BBa_I12038
type: Component
persistent identity: component2238886
display id: component2238886
version: 1
top level: BBa_I12042
definition: BBa_I12038
access: public

BBa_I12002
type: Component
persistent identity: component2238946
display id: component2238946
version: 1
top level: BBa_I12042
definition: BBa_I12002
access: public

BBa_I12038
type: SequenceAnnotation
persistent identity: annotation2238886
display id: annotation2238886
version: 1
top level: BBa_I12042
location: https://synbiohub.org/public/igem/BBa_I12042/annotation2238886/range2238886/1
component: BBa_I12038

https://synbiohub.org/public/igem/BBa_I12042/annotation2238886/range2238886/1
type: Range
persistent identity: range2238886
display id: range2238886
version: 1
top level: BBa_I12042
direction: direction/reverse
start: 1
end: 992
orientation: inline

BBa_I12043
type: SequenceAnnotation
persistent identity: annotation2238925
display id: annotation2238925
version: 1
top level: BBa_I12042
location: https://synbiohub.org/public/igem/BBa_I12042/annotation2238925/range2238925/1
component: BBa_I12043

https://synbiohub.org/public/igem/BBa_I12042/annotation2238925/range2238925/1
type: Range
persistent identity: range2238925
display id: range2238925
version: 1
top level: BBa_I12042
direction: direction/reverse
start: 1001
end: 3102
orientation: inline

BBa_I12002
type: SequenceAnnotation
persistent identity: annotation2238946
display id: annotation2238946
version: 1
top level: BBa_I12042
location: https://synbiohub.org/public/igem/BBa_I12042/annotation2238946/range2238946/1
component: BBa_I12002

https://synbiohub.org/public/igem/BBa_I12042/annotation2238946/range2238946/1
type: Range
persistent identity: range2238946
display id: range2238946
version: 1
top level: BBa_I12042
direction: direction/reverse
start: 3111
end: 4128
orientation: inline

BBa_I12002
type: ComponentDefinition
persistent identity: BBa I12002
display id: BBa_I12002
version: 1
was generated by: iGEM to SBOL conversion
description: Barkai-Leibler design experiment part B (434cI)
created: 2004-07-12T11:00:00Z
modified: 2015-08-31T04:07:31Z
owned by: james
owned by: myers
mutable description: This forms the repressor of a Barkai-Leibler oscillator. The 434 cI has no LVA tag (BBa_I12014 is the same as BBa_C0052 with no LVA tag).
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 147
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This part to work in conjunction with BBa_I12000. This part creates one of the proteins involved in the Modified Barkai-Leibler relaxation oscillator design (repressor protein: 434 cI). In the presence of Lambda cI, this part will create 434 cI, which will in turn repress the production of Lambda cI in BBa_12000. The LVA tag was removed from the 434 cI coding region due to the fact that we want the repressor to be as potent as possible, thus, if it doesn't degrade as quickly, the relative strength should be increased.
star: false
creator: Hans
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I12014
component: BBa_B0034
component: BBa_B0015
component: BBa_I12007
component: BBa_B0025
sequence annotation: BBa_B0025
sequence annotation: BBa_I12007
sequence annotation: BBa_B0034
sequence annotation: BBa_I12014
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_I12002_sequence/1

BBa_I12014
type: Component
persistent identity: component2223483
display id: component2223483
version: 1
top level: BBa_I12002
definition: cI 434
access: public

BBa_B0034
type: Component
persistent identity: component2223481
display id: component2223481
version: 1
top level: BBa_I12002
definition: BBa_B0034
access: public

BBa_B0015
type: Component
persistent identity: component2223490
display id: component2223490
version: 1
top level: BBa_I12002
definition: BBa_B0015
access: public

BBa_I12007
type: Component
persistent identity: component2223479
display id: component2223479
version: 1
top level: BBa_I12002
definition: Prm +
access: public

BBa_B0025
type: Component
persistent identity: component2223473
display id: component2223473
version: 1
top level: BBa_I12002
definition: BBa_B0025
access: public

BBa_B0025
type: SequenceAnnotation
persistent identity: annotation2223473
display id: annotation2223473
version: 1
top level: BBa_I12002
location: https://synbiohub.org/public/igem/BBa_I12002/annotation2223473/range2223473/1
component: BBa_B0025

https://synbiohub.org/public/igem/BBa_I12002/annotation2223473/range2223473/1
type: Range
persistent identity: range2223473
display id: range2223473
version: 1
top level: BBa_I12002
direction: direction/reverse
start: 1
end: 129
orientation: inline

BBa_I12007
type: SequenceAnnotation
persistent identity: annotation2223479
display id: annotation2223479
version: 1
top level: BBa_I12002
location: https://synbiohub.org/public/igem/BBa_I12002/annotation2223479/range2223479/1
component: BBa_I12007

https://synbiohub.org/public/igem/BBa_I12002/annotation2223479/range2223479/1
type: Range
persistent identity: range2223479
display id: range2223479
version: 1
top level: BBa_I12002
direction: direction/reverse
start: 138
end: 219
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2223481
display id: annotation2223481
version: 1
top level: BBa_I12002
location: https://synbiohub.org/public/igem/BBa_I12002/annotation2223481/range2223481/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I12002/annotation2223481/range2223481/1
type: Range
persistent identity: range2223481
display id: range2223481
version: 1
top level: BBa_I12002
direction: direction/reverse
start: 228
end: 239
orientation: inline

BBa_I12014
type: SequenceAnnotation
persistent identity: annotation2223483
display id: annotation2223483
version: 1
top level: BBa_I12002
location: https://synbiohub.org/public/igem/BBa_I12002/annotation2223483/range2223483/1
component: BBa_I12014

https://synbiohub.org/public/igem/BBa_I12002/annotation2223483/range2223483/1
type: Range
persistent identity: range2223483
display id: range2223483
version: 1
top level: BBa_I12002
direction: direction/reverse
start: 246
end: 881
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2223490
display id: annotation2223490
version: 1
top level: BBa_I12002
location: https://synbiohub.org/public/igem/BBa_I12002/annotation2223490/range2223490/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_I12002/annotation2223490/range2223490/1
type: Range
persistent identity: range2223490
display id: range2223490
version: 1
top level: BBa_I12002
direction: direction/reverse
start: 890
end: 1018
orientation: inline

BBa_I12038
type: ComponentDefinition
persistent identity: BBa I12038
display id: BBa_I12038
version: 1
was generated by: iGEM to SBOL conversion
description: Kickstart for the Barkai-Leibler oscillator
created: 2004-08-17T11:00:00Z
modified: 2015-08-31T04:07:31Z
mutable provenance: BioBricks parts
owned by: james
owned by: myers
mutable description: This part is designed for "kickstarting" the Barkai-Leibler oscillator by producing lambda cI.
discontinued: false
dominant: true
experience: experience/None
group u list: _3_
m user id: 0
owner id: 103
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Addition of ATC will repress the tetR and this will result in production of lambda cI.
star: false
creator: Hans
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_C0051
component: BBa_B0034
component: BBa_B0012
component: BBa_B0010
component: BBa_R0040
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence annotation: BBa_C0051
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I12038_sequence/1

BBa_C0051
type: Component
persistent identity: component1056895
display id: component1056895
version: 1
top level: BBa_I12038
definition: cI lam
access: public

BBa_B0034
type: Component
persistent identity: component1056885
display id: component1056885
version: 1
top level: BBa_I12038
definition: BBa_B0034
access: public

BBa_B0012
type: Component
persistent identity: component1056911
display id: component1056911
version: 1
top level: BBa_I12038
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1056901
display id: component1056901
version: 1
top level: BBa_I12038
definition: BBa_B0010
access: public

BBa_R0040
type: Component
persistent identity: component1056877
display id: component1056877
version: 1
top level: BBa_I12038
definition: p(tetR)
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1056877
display id: annotation1056877
version: 1
top level: BBa_I12038
location: https://synbiohub.org/public/igem/BBa_I12038/annotation1056877/range1056877/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_I12038/annotation1056877/range1056877/1
type: Range
persistent identity: range1056877
display id: range1056877
version: 1
top level: BBa_I12038
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1056885
display id: annotation1056885
version: 1
top level: BBa_I12038
location: https://synbiohub.org/public/igem/BBa_I12038/annotation1056885/range1056885/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I12038/annotation1056885/range1056885/1
type: Range
persistent identity: range1056885
display id: range1056885
version: 1
top level: BBa_I12038
direction: direction/forward
start: 63
end: 74
orientation: inline

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation1056895
display id: annotation1056895
version: 1
top level: BBa_I12038
location: https://synbiohub.org/public/igem/BBa_I12038/annotation1056895/range1056895/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_I12038/annotation1056895/range1056895/1
type: Range
persistent identity: range1056895
display id: range1056895
version: 1
top level: BBa_I12038
direction: direction/forward
start: 81
end: 830
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1056901
display id: annotation1056901
version: 1
top level: BBa_I12038
location: https://synbiohub.org/public/igem/BBa_I12038/annotation1056901/range1056901/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I12038/annotation1056901/range1056901/1
type: Range
persistent identity: range1056901
display id: range1056901
version: 1
top level: BBa_I12038
direction: direction/forward
start: 864
end: 943
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1056911
display id: annotation1056911
version: 1
top level: BBa_I12038
location: https://synbiohub.org/public/igem/BBa_I12038/annotation1056911/range1056911/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I12038/annotation1056911/range1056911/1
type: Range
persistent identity: range1056911
display id: range1056911
version: 1
top level: BBa_I12038
direction: direction/forward
start: 952
end: 992
orientation: inline

BBa_I12043
type: ComponentDefinition
persistent identity: BBa I12043
display id: BBa_I12043
version: 1
was generated by: iGEM to SBOL conversion
description: Activator for BL oscillator with reporter protein
created: 2004-08-19T11:00:00Z
modified: 2015-08-31T04:07:31Z
owned by: james
owned by: myers
mutable description: This part forms the activator part of the Barkai-Leibler oscillator. It has no cooperativity for the repressor protein 434 cI (one OR site). It includes a reporter protein.
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 103
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This part has no cooperativity for the repressor 434 cI (only one 434 OR site). A reporter protein (EYFP) coding region is placed after the lambda cI coding region. This will make it possible to keep track of the lambda cI levels in the cell.
star: false
creator: Hans
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_B0034
component: BBa_C0051
component: BBa_B0015
component: BBa_E0432
component: BBa_B0025
component: BBa_I12006
sequence annotation: BBa_B0025
sequence annotation: BBa_I12006
sequence annotation: BBa_B0034
sequence annotation: BBa_C0051
sequence annotation: BBa_E0432
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_I12043_sequence/1

BBa_B0034
type: Component
persistent identity: component2231746
display id: component2231746
version: 1
top level: BBa_I12043
definition: BBa_B0034
access: public

BBa_C0051
type: Component
persistent identity: component2231744
display id: component2231744
version: 1
top level: BBa_I12043
definition: cI lam
access: public

BBa_B0015
type: Component
persistent identity: component2231767
display id: component2231767
version: 1
top level: BBa_I12043
definition: BBa_B0015
access: public

BBa_E0432
type: Component
persistent identity: component2231760
display id: component2231760
version: 1
top level: BBa_I12043
definition: BBa_E0432
access: public

BBa_B0025
type: Component
persistent identity: component2231732
display id: component2231732
version: 1
top level: BBa_I12043
definition: BBa_B0025
access: public

BBa_B0034
type: Component
persistent identity: component2231740
display id: component2231740
version: 1
top level: BBa_I12043
definition: BBa_B0034
access: public

BBa_I12006
type: Component
persistent identity: component2231738
display id: component2231738
version: 1
top level: BBa_I12043
definition: Prm +
access: public

BBa_B0025
type: SequenceAnnotation
persistent identity: annotation2231732
display id: annotation2231732
version: 1
top level: BBa_I12043
location: https://synbiohub.org/public/igem/BBa_I12043/annotation2231732/range2231732/1
component: BBa_B0025

https://synbiohub.org/public/igem/BBa_I12043/annotation2231732/range2231732/1
type: Range
persistent identity: range2231732
display id: range2231732
version: 1
top level: BBa_I12043
direction: direction/reverse
start: 1
end: 129
orientation: inline

BBa_I12006
type: SequenceAnnotation
persistent identity: annotation2231738
display id: annotation2231738
version: 1
top level: BBa_I12043
location: https://synbiohub.org/public/igem/BBa_I12043/annotation2231738/range2231738/1
component: BBa_I12006

https://synbiohub.org/public/igem/BBa_I12043/annotation2231738/range2231738/1
type: Range
persistent identity: range2231738
display id: range2231738
version: 1
top level: BBa_I12043
direction: direction/reverse
start: 138
end: 219
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2231740
display id: annotation2231740
version: 1
top level: BBa_I12043
location: https://synbiohub.org/public/igem/BBa_I12043/annotation2231740/range2231740/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I12043/annotation2231740/range2231740/1
type: Range
persistent identity: range2231740
display id: range2231740
version: 1
top level: BBa_I12043
direction: direction/reverse
start: 228
end: 239
orientation: inline

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation2231744
display id: annotation2231744
version: 1
top level: BBa_I12043
location: https://synbiohub.org/public/igem/BBa_I12043/annotation2231744/range2231744/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_I12043/annotation2231744/range2231744/1
type: Range
persistent identity: range2231744
display id: range2231744
version: 1
top level: BBa_I12043
direction: direction/reverse
start: 246
end: 1020
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2231746
display id: annotation2231746
version: 1
top level: BBa_I12043
location: https://synbiohub.org/public/igem/BBa_I12043/annotation2231746/range2231746/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I12043/annotation2231746/range2231746/1
type: Range
persistent identity: range2231746
display id: range2231746
version: 1
top level: BBa_I12043
direction: direction/reverse
start: 1029
end: 1040
orientation: inline

BBa_E0432
type: SequenceAnnotation
persistent identity: annotation2231760
display id: annotation2231760
version: 1
top level: BBa_I12043
location: https://synbiohub.org/public/igem/BBa_I12043/annotation2231760/range2231760/1
component: BBa_E0432

https://synbiohub.org/public/igem/BBa_I12043/annotation2231760/range2231760/1
type: Range
persistent identity: range2231760
display id: range2231760
version: 1
top level: BBa_I12043
direction: direction/reverse
start: 1049
end: 1965
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2231767
display id: annotation2231767
version: 1
top level: BBa_I12043
location: https://synbiohub.org/public/igem/BBa_I12043/annotation2231767/range2231767/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_I12043/annotation2231767/range2231767/1
type: Range
persistent identity: range2231767
display id: range2231767
version: 1
top level: BBa_I12043
direction: direction/reverse
start: 1974
end: 2102
orientation: inline

cI 434
type: ComponentDefinition
persistent identity: BBa I12014
display id: BBa_I12014
version: 1
was generated by: iGEM to SBOL conversion
description: Repressor, 434 cI (RBS- LVA-)
created: 2004-07-20T11:00:00Z
modified: 2015-08-31T04:07:31Z
mutable provenance: Nikolnikov,S., Posfai,G. and Sain,B. "The construction of a versatile plasmid vector that allows direct selection of fragments cloned into six unique sites of the cI gene of coliphage 434"
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The 434 cI repressor protein coding sequence is a 710 base-pair sequence with the standard RBS-compatible BioBrick prefix and the standard BioBrick suffix sections on its ends. It binds to the 434 regulatory sequence, BBa_R0052. The sequence has no LVA tag nor RBS.
discontinued: false
dominant: true
experience: experience/None
group u list: _3_
m user id: 0
owner id: 148
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Essentially the same as BBa_C0052 but with the LVA tag removed.
star: false
creator: ryhsiao
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: cI 434
sequence: https://synbiohub.org/public/igem/BBa_I12014_sequence/1

cI 434
type: SequenceAnnotation
persistent identity: annotation912810
display id: annotation912810
version: 1
top level: cI 434
location: https://synbiohub.org/public/igem/BBa_I12014/annotation912810/range912810/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I12014/annotation912810/range912810/1
type: Range
persistent identity: range912810
display id: range912810
version: 1
top level: cI 434
direction: direction/forward
start: 1
end: 669
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

cI lam
type: ComponentDefinition
persistent identity: BBa C0051
display id: BBa_C0051
version: 1
was generated by: iGEM to SBOL conversion
description: cI repressor from E. coli phage lambda (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999).
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the cI repressor based on cI repressor from bacteriophage lambda modified with an LVA tail for rapid degradation of the protein. cI repressor binds to the cI regulator (BBa_R0051).
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001 References (unparsed) here: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001 BBa_C0051 cI repressor is based on the cI repressor from the Elowitz's repressilator. It has been modified to include a rapid degradation LAA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cI lambda
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0051_sequence/1

cI lambda
type: SequenceAnnotation
persistent identity: annotation23334
display id: annotation23334
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation23334/range23334/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0051/annotation23334/range23334/1
type: Range
persistent identity: range23334
display id: range23334
version: 1
top level: cI lam
direction: direction/forward
start: 4
end: 711
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation23335
display id: annotation23335
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation23335/range23335/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0051/annotation23335/range23335/1
type: Range
persistent identity: range23335
display id: range23335
version: 1
top level: cI lam
direction: direction/forward
start: 712
end: 744
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213991
display id: annotation2213991
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation2213991/range2213991/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0051/annotation2213991/range2213991/1
type: Range
persistent identity: range2213991
display id: range2213991
version: 1
top level: cI lam
direction: direction/forward
start: 751
end: 775
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_B0025
type: ComponentDefinition
persistent identity: BBa B0025
display id: BBa_B0025
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0015), reversed
created: 2003-12-02T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: -- No description --
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Caitlin Conboy
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: B0012
sequence annotation: B0010
sequence: https://synbiohub.org/public/igem/BBa_B0025_sequence/1

B0012
type: SequenceAnnotation
persistent identity: annotation369702
display id: annotation369702
version: 1
top level: BBa_B0025
location: https://synbiohub.org/public/igem/BBa_B0025/annotation369702/range369702/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0025/annotation369702/range369702/1
type: Range
persistent identity: range369702
display id: range369702
version: 1
top level: BBa_B0025
direction: direction/reverse
start: 1
end: 41
orientation: inline

B0010
type: SequenceAnnotation
persistent identity: annotation369703
display id: annotation369703
version: 1
top level: BBa_B0025
location: https://synbiohub.org/public/igem/BBa_B0025/annotation369703/range369703/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0025/annotation369703/range369703/1
type: Range
persistent identity: range369703
display id: range369703
version: 1
top level: BBa_B0025
direction: direction/reverse
start: 50
end: 129
orientation: inline

Prm +
type: ComponentDefinition
persistent identity: BBa I12007
display id: BBa_I12007
version: 1
was generated by: iGEM to SBOL conversion
description: Modified lambda Prm promoter (OR-3 obliterated)
created: 2004-07-14T11:00:00Z
modified: 2015-08-31T04:07:31Z
mutable provenance: Shih and Gussin (1983)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Lambda Prm promoter modified to be activated but not repressed by the lambda repressor (cI)
discontinued: false
dominant: true
experience: experience/None
group u list: _3_
m user id: 0
owner id: 103
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In wild type lambda phage, the OR3 site (-27 to -11) reads "tatcccttgcggtgata" on the sense strand of DNA. To prevent lambda repressor (cI) from binding to this site, the 4th through 10th nt of OR3 were replaced by the 7 nt between OR2 and OR1, "aaatagt" (-57 to -51), which in effect mutates 5 nt of the promoter. These nt were chosen to be about halfway between the -10 and -35 boxes of the promoter. Furthermore, since these nt already act as a spacer in wild-type phage, it is hoped they will not create undesired interactions.
star: true
creator: Hans
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OR1
sequence annotation: OR2
sequence annotation: -35
sequence annotation: mutate to obliterate OR3
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_I12007_sequence/1

OR1
type: SequenceAnnotation
persistent identity: annotation937701
display id: annotation937701
version: 1
top level: Prm +
location: https://synbiohub.org/public/igem/BBa_I12007/annotation937701/range937701/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_I12007/annotation937701/range937701/1
type: Range
persistent identity: range937701
display id: range937701
version: 1
top level: Prm +
direction: direction/forward
start: 9
end: 25
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation937702
display id: annotation937702
version: 1
top level: Prm +
location: https://synbiohub.org/public/igem/BBa_I12007/annotation937702/range937702/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_I12007/annotation937702/range937702/1
type: Range
persistent identity: range937702
display id: range937702
version: 1
top level: Prm +
direction: direction/forward
start: 33
end: 49
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation937703
display id: annotation937703
version: 1
top level: Prm +
location: https://synbiohub.org/public/igem/BBa_I12007/annotation937703/range937703/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I12007/annotation937703/range937703/1
type: Range
persistent identity: range937703
display id: range937703
version: 1
top level: Prm +
direction: direction/forward
start: 48
end: 53
orientation: inline

mutate to obliterate OR3
type: SequenceAnnotation
persistent identity: annotation937704
display id: annotation937704
version: 1
top level: Prm +
location: https://synbiohub.org/public/igem/BBa_I12007/annotation937704/range937704/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_I12007/annotation937704/range937704/1
type: Range
persistent identity: range937704
display id: range937704
version: 1
top level: Prm +
direction: direction/forward
start: 59
end: 65
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation937705
display id: annotation937705
version: 1
top level: Prm +
location: https://synbiohub.org/public/igem/BBa_I12007/annotation937705/range937705/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I12007/annotation937705/range937705/1
type: Range
persistent identity: range937705
display id: range937705
version: 1
top level: Prm +
direction: direction/forward
start: 71
end: 76
orientation: inline

BBa_E0432
type: ComponentDefinition
persistent identity: BBa E0432
display id: BBa_E0432
version: 1
was generated by: iGEM to SBOL conversion
description: EYFP (RBS+ LVA+ TERM) (B0034.E0032.B0015)
created: 2003-12-04T12:00:00Z
modified: 2015-08-31T04:07:26Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: -- No description --
discontinued: false
dominant: true
experience: experience/Issues
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: Caitlin Conboy and Jennifer Braff
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0012
component: BBa_B0010
component: BBa_E0032
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_E0032
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_E0432_sequence/1

BBa_B0012
type: Component
persistent identity: component942938
display id: component942938
version: 1
top level: BBa_E0432
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component942928
display id: component942928
version: 1
top level: BBa_E0432
definition: BBa_B0010
access: public

BBa_E0032
type: Component
persistent identity: component942920
display id: component942920
version: 1
top level: BBa_E0432
definition: YFP
access: public

BBa_B0034
type: Component
persistent identity: component942908
display id: component942908
version: 1
top level: BBa_E0432
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation942908
display id: annotation942908
version: 1
top level: BBa_E0432
location: https://synbiohub.org/public/igem/BBa_E0432/annotation942908/range942908/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_E0432/annotation942908/range942908/1
type: Range
persistent identity: range942908
display id: range942908
version: 1
top level: BBa_E0432
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_E0032
type: SequenceAnnotation
persistent identity: annotation942920
display id: annotation942920
version: 1
top level: BBa_E0432
location: https://synbiohub.org/public/igem/BBa_E0432/annotation942920/range942920/1
component: BBa_E0032

https://synbiohub.org/public/igem/BBa_E0432/annotation942920/range942920/1
type: Range
persistent identity: range942920
display id: range942920
version: 1
top level: BBa_E0432
direction: direction/forward
start: 19
end: 780
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation942928
display id: annotation942928
version: 1
top level: BBa_E0432
location: https://synbiohub.org/public/igem/BBa_E0432/annotation942928/range942928/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_E0432/annotation942928/range942928/1
type: Range
persistent identity: range942928
display id: range942928
version: 1
top level: BBa_E0432
direction: direction/forward
start: 789
end: 868
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation942938
display id: annotation942938
version: 1
top level: BBa_E0432
location: https://synbiohub.org/public/igem/BBa_E0432/annotation942938/range942938/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_E0432/annotation942938/range942938/1
type: Range
persistent identity: range942938
display id: range942938
version: 1
top level: BBa_E0432
direction: direction/forward
start: 877
end: 917
orientation: inline

Prm +
type: ComponentDefinition
persistent identity: BBa I12006
display id: BBa_I12006
version: 1
was generated by: iGEM to SBOL conversion
description: Modified lamdba Prm promoter (repressed by 434 cI)
created: 2004-07-13T11:00:00Z
modified: 2015-08-31T04:07:31Z
mutable provenance: Bushman(1993), Shih & Gussin (1983)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Lamdba Prm promoter modified to be activated by lamda repressor (cI) and repressed by 434 repressor (cI)
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 147
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The O-R1 region of 434 contained 14 base pairs as opposed to the 17 base pairs of the O-R3 site of lambda. Also, it was noticed that the O-R3 site of the lambda included part of the -10 site. Hence, to preserve the spacing and the -10 site, the three nucleotides that were in both the -10 site and the lambda O-R3 site were retained. The 14 nucleotides that were in the O-R3 site and not in the -10 site were replaced with the O-R1 site of the 434.
star: true
creator: mcnamara
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OR1 lambda
sequence annotation: OR2 lambda
sequence annotation: -35
sequence annotation: OR1 434
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_I12006_sequence/1

OR1 lambda
type: SequenceAnnotation
persistent identity: annotation786500
display id: annotation786500
version: 1
top level: Prm +
location: https://synbiohub.org/public/igem/BBa_I12006/annotation786500/range786500/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_I12006/annotation786500/range786500/1
type: Range
persistent identity: range786500
display id: range786500
version: 1
top level: Prm +
direction: direction/forward
start: 9
end: 25
orientation: inline

OR2 lambda
type: SequenceAnnotation
persistent identity: annotation786518
display id: annotation786518
version: 1
top level: Prm +
location: https://synbiohub.org/public/igem/BBa_I12006/annotation786518/range786518/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_I12006/annotation786518/range786518/1
type: Range
persistent identity: range786518
display id: range786518
version: 1
top level: Prm +
direction: direction/forward
start: 33
end: 49
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation837284
display id: annotation837284
version: 1
top level: Prm +
location: https://synbiohub.org/public/igem/BBa_I12006/annotation837284/range837284/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I12006/annotation837284/range837284/1
type: Range
persistent identity: range837284
display id: range837284
version: 1
top level: Prm +
direction: direction/forward
start: 48
end: 53
orientation: inline

OR1 434
type: SequenceAnnotation
persistent identity: annotation786365
display id: annotation786365
version: 1
top level: Prm +
location: https://synbiohub.org/public/igem/BBa_I12006/annotation786365/range786365/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_I12006/annotation786365/range786365/1
type: Range
persistent identity: range786365
display id: range786365
version: 1
top level: Prm +
direction: direction/forward
start: 56
end: 69
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation837228
display id: annotation837228
version: 1
top level: Prm +
location: https://synbiohub.org/public/igem/BBa_I12006/annotation837228/range837228/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I12006/annotation837228/range837228/1
type: Range
persistent identity: range837228
display id: range837228
version: 1
top level: Prm +
direction: direction/forward
start: 71
end: 76
orientation: inline

YFP
type: ComponentDefinition
persistent identity: BBa E0032
display id: BBa_E0032
version: 1
was generated by: iGEM to SBOL conversion
description: enhanced yellow fluorescent protein derived from A. victoria GFP
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:25Z
mutable provenance: Modified from <bb_part>BBa_E0031</bb_part>
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Yellow fluorescent protein (EYFP) reporter coding sequence without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. Annotated mutation cause a Q81L mutation that appears to not be near the active site.
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_E0032 yellow fluorescent protein is based on BioBrick part BBa_E0031. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000804
role: partType/Reporter
sequence annotation: YFP (LVA)
sequence annotation: BBa_E0032
sequence annotation: A (Q->L)
sequence annotation: SsrA
sequence annotation: 2
sequence: https://synbiohub.org/public/igem/BBa_E0032_sequence/1

YFP (LVA)
type: SequenceAnnotation
persistent identity: annotation2156
display id: annotation2156
version: 1
top level: YFP
location: https://synbiohub.org/public/igem/BBa_E0032/annotation2156/range2156/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E0032/annotation2156/range2156/1
type: Range
persistent identity: range2156
display id: range2156
version: 1
top level: YFP
direction: direction/forward
start: 1
end: 762
orientation: inline

BBa_E0032
type: SequenceAnnotation
persistent identity: annotation7041
display id: annotation7041
version: 1
top level: YFP
location: https://synbiohub.org/public/igem/BBa_E0032/annotation7041/range7041/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_E0032/annotation7041/range7041/1
type: Range
persistent identity: range7041
display id: range7041
version: 1
top level: YFP
direction: direction/forward
start: 1
end: 762
orientation: inline

A (Q->L)
type: SequenceAnnotation
persistent identity: annotation2161
display id: annotation2161
version: 1
top level: YFP
location: https://synbiohub.org/public/igem/BBa_E0032/annotation2161/range2161/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_E0032/annotation2161/range2161/1
type: Range
persistent identity: range2161
display id: range2161
version: 1
top level: YFP
direction: direction/forward
start: 242
end: 242
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation2160
display id: annotation2160
version: 1
top level: YFP
location: https://synbiohub.org/public/igem/BBa_E0032/annotation2160/range2160/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_E0032/annotation2160/range2160/1
type: Range
persistent identity: range2160
display id: range2160
version: 1
top level: YFP
direction: direction/forward
start: 718
end: 756
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation2159
display id: annotation2159
version: 1
top level: YFP
location: https://synbiohub.org/public/igem/BBa_E0032/annotation2159/range2159/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_E0032/annotation2159/range2159/1
type: Range
persistent identity: range2159
display id: range2159
version: 1
top level: YFP
direction: direction/forward
start: 757
end: 762
orientation: inline

https://synbiohub.org/public/igem/BBa_I12042_sequence/1
type: Sequence
persistent identity: BBa I12042 sequence
display id: BBa_I12042_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggtactagaggcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttagatattacaaactttcttgtatagatttaacgttactagagaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I12002_sequence/1
type: Sequence
persistent identity: BBa I12002 sequence
display id: BBa_I12002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggtactagaggcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttagatatttataaatagtggtgatagatttaacgttactagagaaagaggagaaatactagatgagtatttcttccagggtaaaaagcaaaagaatccagcttggacttaaccaggctgaacttgctcaaaaggtggggactacccagcagtctatagagcagctcgaaaacggtaaaactaagcgaccacgctttttaccagaacttgcgtcagctcttggcgtaagtgttgactggctgctcaatggcacctctgattcgaatgttagatttgttgggcacgttgagcccaaagggaaatatccattgattagcatggttagagctggttcgtggtgtgaagcttgtgaaccctacgatatcaaggacattgatgaatggtatgacagtgacgttaacttattaggcaatggattctggctgaaggttgaaggtgattccatgacctcacctgtaggtcaaagcatccctgaaggtcatatggtgttagtagatactggacgggagccagtgaatggaagccttgttgtagccaaactgactgacgcgaacgaagcaacattcaagaaactggtcatagatggcggtcagaagtacctgaaaggcctgaatccttcatggcctatgactcctatcaacggaaactgcaagattatcggtgttgtcgtggaagcgagggtaaaattcgtataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I12038_sequence/1
type: Sequence
persistent identity: BBa I12038 sequence
display id: BBa_I12038_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I12043_sequence/1
type: Sequence
persistent identity: BBa I12043 sequence
display id: BBa_I12043_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggtactagaggcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttagatattacaaactttcttgtatagatttaacgttactagagaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagaaagaggagaaatactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagccaggcatcaaataaaacgaaaggctc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I12014_sequence/1
type: Sequence
persistent identity: BBa I12014 sequence
display id: BBa_I12014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagtatttcttccagggtaaaaagcaaaagaatccagcttggacttaaccaggctgaacttgctcaaaaggtggggactacccagcagtctatagagcagctcgaaaacggtaaaactaagcgaccacgctttttaccagaacttgcgtcagctcttggcgtaagtgttgactggctgctcaatggcacctctgattcgaatgttagatttgttgggcacgttgagcccaaagggaaatatccattgattagcatggttagagctggttcgtggtgtgaagcttgtgaaccctacgatatcaaggacattgatgaatggtatgacagtgacgttaacttattaggcaatggattctggctgaaggttgaaggtgattccatgacctcacctgtaggtcaaagcatccctgaaggtcatatggtgttagtagatactggacgggagccagtgaatggaagccttgttgtagccaaactgactgacgcgaacgaagcaacattcaagaaactggtcatagatggcggtcagaagtacctgaaaggcctgaatccttcatggcctatgactcctatcaacggaaactgcaagattatcggtgttgtcgtggaagcgagggtaaaattcgtataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0051_sequence/1
type: Sequence
persistent identity: BBa C0051 sequence
display id: BBa_C0051_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0025_sequence/1
type: Sequence
persistent identity: BBa B0025 sequence
display id: BBa_B0025_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctgg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I12007_sequence/1
type: Sequence
persistent identity: BBa I12007 sequence
display id: BBa_I12007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttagatatttataaatagtggtgatagatttaacgt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0432_sequence/1
type: Sequence
persistent identity: BBa E0432 sequence
display id: BBa_E0432_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I12006_sequence/1
type: Sequence
persistent identity: BBa I12006 sequence
display id: BBa_I12006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttagatattacaaactttcttgtatagatttaacgt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0032_sequence/1
type: Sequence
persistent identity: BBa E0032 sequence
display id: BBa_E0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z