LOCUS       BBa_I12043              2102 bp    DNA     linear       02-May-2024
DEFINITION  Activator for BL oscillator with reporter protein
ACCESSION   BBa_I12043
VERSION     BBa_I12043.1  
FEATURES             Location/Qualifiers
     misc_feature    1..41
                     /label=B0012
     misc_feature    50..129
                     /label=B0010
     terminator      1..129
                     /label=BBa_B0025
     misc_feature    193..206
                     /label=OR1 434
     misc_feature    146..162
                     /label=OR1 lambda
     misc_feature    170..186
                     /label=OR2 lambda
     promoter        208..213
                     /label=-10
     promoter        185..190
                     /label=-35
     promoter        138..219
                     /label=BBa_I12006
     misc_feature    232..235
                     /label=conserved
     RBS             228..239
                     /label=BBa_B0034
     CDS             249..956
                     /label=cI lambda
     misc_feature    957..989
                     /label=LVA
     misc_feature    996..1020
                     /label=Help:Barcodes
     CDS             246..1020
                     /label=BBa_C0051
     misc_feature    1033..1036
                     /label=conserved
     RBS             1029..1040
                     /label=BBa_B0034
     misc_feature    1053..1056
                     /label=conserved
     RBS             1049..1060
                     /label=BBa_B0034
     CDS             1067..1828
                     /label=YFP (LVA)
     misc_feature    1823..1828
                     /label=2
     misc_feature    1784..1822
                     /label=SsrA
     misc_feature    1308..1308
                     /label=A (Q->L)
     misc_feature    1067..1828
                     /label=BBa_E0032
     misc_feature    1067..1828
                     /label=BBa_E0032
     stem_loop       1848..1891
                     /label=stem_loop
     misc_feature    1837..1916
                     /label=BBa_B0010
     terminator      1837..1916
                     /label=BBa_B0010
     stem_loop       1932..1951
                     /label=T7 TE
     misc_feature    1958..1958
                     /label=stop
     polyA_site      1952..1965
                     /label=polya
     misc_feature    1925..1965
                     /label=BBa_B0012
     terminator      1925..1965
                     /label=BBa_B0012
     misc_feature    1049..1965
                     /label=BBa_E0432
     stem_loop       1985..2028
                     /label=stem_loop
     misc_feature    1974..2053
                     /label=BBa_B0010
     terminator      1974..2053
                     /label=BBa_B0010
     stem_loop       2069..2088
                     /label=T7 TE
     misc_feature    2095..2095
                     /label=stop
     polyA_site      2089..2102
                     /label=polya
     misc_feature    2062..2102
                     /label=BBa_B0012
     terminator      2062..2102
                     /label=BBa_B0012
     terminator      1974..2102
                     /label=BBa_B0015
ORIGIN
        1 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca
       61 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt
      121 gatgcctggt actagaggca accattatca ccgccagagg taaaatagtc aacacgcacg
      181 gtgttagata ttacaaactt tcttgtatag atttaacgtt actagagaaa gaggagaaat
      241 actagatgag cacaaaaaag aaaccattaa cacaagagca gcttgaggac gcacgtcgcc
      301 ttaaagcaat ttatgaaaaa aagaaaaatg aacttggctt atcccaggaa tctgtcgcag
      361 acaagatggg gatggggcag tcaggcgttg gtgctttatt taatggcatc aatgcattaa
      421 atgcttataa cgccgcattg cttgcaaaaa ttctcaaagt tagcgttgaa gaatttagcc
      481 cttcaatcgc cagagaaatc tacgagatgt atgaagcggt tagtatgcag ccgtcactta
      541 gaagtgagta tgagtaccct gttttttctc atgttcaggc agggatgttc tcacctgagc
      601 ttagaacctt taccaaaggt gatgcggaga gatgggtaag cacaaccaaa aaagccagtg
      661 attctgcatt ctggcttgag gttgaaggta attccatgac cgcaccaaca ggctccaagc
      721 caagctttcc tgacggaatg ttaattctcg ttgaccctga gcaggctgtt gagccaggtg
      781 atttctgcat agccagactt gggggtgatg agtttacctt caagaaactg atcagggata
      841 gcggtcaggt gtttttacaa ccactaaacc cacagtaccc aatgatccca tgcaatgaga
      901 gttgttccgt tgtggggaaa gttatcgcta gtcagtggcc tgaagagacg tttggcgctg
      961 caaacgacga aaactacgct ttagtagctt aataacgctg atagtgctag tgtagatcgc
     1021 tactagagaa agaggagaaa tactagagaa agaggagaaa tactagatgg tgagcaaggg
     1081 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
     1141 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
     1201 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt
     1261 cggctacggc ctgcaatgct tcgcccgcta ccccgaccac atgaagctgc acgacttctt
     1321 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
     1381 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     1441 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     1501 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
     1561 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
     1621 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagctacca
     1681 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     1741 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagaggcctg ctgcaaacga
     1801 cgaaaactac gctttagtag cttaataata ctagagccag gcatcaaata aaacgaaagg
     1861 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
     1921 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagccaggca
     1981 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
     2041 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta
     2101 ta
//