LOCUS       BBa_I12046              2111 bp    DNA     linear       25-Nov-2024
DEFINITION  Activator for BL oscillator with reporter protein, (cooperativity 
            and L-strain -10 region)
ACCESSION   BBa_I12046
VERSION     BBa_I12046.1  
FEATURES             Location/Qualifiers
     misc_feature    1..41
                     /label=B0012
     misc_feature    50..129
                     /label=B0010
     terminator      1..129
                     /label=BBa_B0025
     misc_feature    146..162
                     /label=OR1 lambda
     misc_feature    170..186
                     /label=OR2 lambda
     promoter        185..190
                     /label=-35
     misc_feature    193..206
                     /label=OR1 434
     promoter        208..213
                     /label=-10
     misc_feature    215..228
                     /label=OR2 434
     misc_feature    220..220
                     /label=start
     promoter        138..228
                     /label=BBa_I12040
     misc_feature    241..244
                     /label=conserved
     RBS             237..248
                     /label=BBa_B0034
     CDS             258..965
                     /label=cI lambda
     misc_feature    966..998
                     /label=LVA
     misc_feature    1005..1029
                     /label=Help:Barcodes
     CDS             255..1029
                     /label=BBa_C0051
     misc_feature    1042..1045
                     /label=conserved
     RBS             1038..1049
                     /label=BBa_B0034
     misc_feature    1062..1065
                     /label=conserved
     RBS             1058..1069
                     /label=BBa_B0034
     CDS             1076..1837
                     /label=YFP (LVA)
     misc_feature    1832..1837
                     /label=2
     misc_feature    1793..1831
                     /label=SsrA
     misc_feature    1317..1317
                     /label=A (Q->L)
     misc_feature    1076..1837
                     /label=BBa_E0032
     misc_feature    1076..1837
                     /label=BBa_E0032
     stem_loop       1857..1900
                     /label=stem_loop
     misc_feature    1846..1925
                     /label=BBa_B0010
     terminator      1846..1925
                     /label=BBa_B0010
     stem_loop       1941..1960
                     /label=T7 TE
     misc_feature    1967..1967
                     /label=stop
     polyA_site      1961..1974
                     /label=polya
     misc_feature    1934..1974
                     /label=BBa_B0012
     terminator      1934..1974
                     /label=BBa_B0012
     misc_feature    1058..1974
                     /label=BBa_E0432
     stem_loop       1994..2037
                     /label=stem_loop
     misc_feature    1983..2062
                     /label=BBa_B0010
     terminator      1983..2062
                     /label=BBa_B0010
     stem_loop       2078..2097
                     /label=T7 TE
     misc_feature    2104..2104
                     /label=stop
     polyA_site      2098..2111
                     /label=polya
     misc_feature    2071..2111
                     /label=BBa_B0012
     terminator      2071..2111
                     /label=BBa_B0012
     terminator      1983..2111
                     /label=BBa_B0015
ORIGIN
        1 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca
       61 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt
      121 gatgcctggt actagaggca accattatca ccgccagagg taaaatagtc aacacgcacg
      181 gtgttagata ttacaaactt tcttgtagat acttacaatg tatcttgtta ctagagaaag
      241 aggagaaata ctagatgagc acaaaaaaga aaccattaac acaagagcag cttgaggacg
      301 cacgtcgcct taaagcaatt tatgaaaaaa agaaaaatga acttggctta tcccaggaat
      361 ctgtcgcaga caagatgggg atggggcagt caggcgttgg tgctttattt aatggcatca
      421 atgcattaaa tgcttataac gccgcattgc ttgcaaaaat tctcaaagtt agcgttgaag
      481 aatttagccc ttcaatcgcc agagaaatct acgagatgta tgaagcggtt agtatgcagc
      541 cgtcacttag aagtgagtat gagtaccctg ttttttctca tgttcaggca gggatgttct
      601 cacctgagct tagaaccttt accaaaggtg atgcggagag atgggtaagc acaaccaaaa
      661 aagccagtga ttctgcattc tggcttgagg ttgaaggtaa ttccatgacc gcaccaacag
      721 gctccaagcc aagctttcct gacggaatgt taattctcgt tgaccctgag caggctgttg
      781 agccaggtga tttctgcata gccagacttg ggggtgatga gtttaccttc aagaaactga
      841 tcagggatag cggtcaggtg tttttacaac cactaaaccc acagtaccca atgatcccat
      901 gcaatgagag ttgttccgtt gtggggaaag ttatcgctag tcagtggcct gaagagacgt
      961 ttggcgctgc aaacgacgaa aactacgctt tagtagctta ataacgctga tagtgctagt
     1021 gtagatcgct actagagaaa gaggagaaat actagagaaa gaggagaaat actagatggt
     1081 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     1141 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     1201 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     1261 gaccaccttc ggctacggcc tgcaatgctt cgcccgctac cccgaccaca tgaagctgca
     1321 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     1381 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     1441 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     1501 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
     1561 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
     1621 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     1681 gagctaccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     1741 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agaggcctgc
     1801 tgcaaacgac gaaaactacg ctttagtagc ttaataatac tagagccagg catcaaataa
     1861 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
     1921 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag
     1981 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
     2041 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt
     2101 ctgcgtttat a
//