LOCUS BBa_I12047 4137 bp DNA linear 22-Nov-2024 DEFINITION BL oscillator, cooperativity + replaced -10 region (Llac), reporter protein, kickstart ACCESSION BBa_I12047 VERSION BBa_I12047.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 CDS 84..791 /label=cI lambda misc_feature 792..824 /label=LVA misc_feature 831..855 /label=Help:Barcodes CDS 81..830 /label=BBa_C0051 stem_loop 875..918 /label=stem_loop misc_feature 864..943 /label=BBa_B0010 terminator 864..943 /label=BBa_B0010 stem_loop 959..978 /label=T7 TE misc_feature 985..985 /label=stop polyA_site 979..992 /label=polya misc_feature 952..992 /label=BBa_B0012 terminator 952..992 /label=BBa_B0012 misc_feature 1..992 /label=BBa_I12038 misc_feature 1001..1041 /label=B0012 misc_feature 1050..1129 /label=B0010 terminator 1001..1129 /label=BBa_B0025 misc_feature 1146..1162 /label=OR1 lambda misc_feature 1170..1186 /label=OR2 lambda promoter 1185..1190 /label=-35 misc_feature 1193..1206 /label=OR1 434 promoter 1208..1213 /label=-10 misc_feature 1215..1228 /label=OR2 434 misc_feature 1220..1220 /label=start promoter 1138..1228 /label=BBa_I12040 misc_feature 1241..1244 /label=conserved RBS 1237..1248 /label=BBa_B0034 CDS 1258..1965 /label=cI lambda misc_feature 1966..1998 /label=LVA misc_feature 2005..2029 /label=Help:Barcodes CDS 1255..2029 /label=BBa_C0051 misc_feature 2042..2045 /label=conserved RBS 2038..2049 /label=BBa_B0034 misc_feature 2062..2065 /label=conserved RBS 2058..2069 /label=BBa_B0034 CDS 2076..2837 /label=YFP (LVA) misc_feature 2832..2837 /label=2 misc_feature 2793..2831 /label=SsrA misc_feature 2317..2317 /label=A (Q->L) misc_feature 2076..2837 /label=BBa_E0032 misc_feature 2076..2837 /label=BBa_E0032 stem_loop 2857..2900 /label=stem_loop misc_feature 2846..2925 /label=BBa_B0010 terminator 2846..2925 /label=BBa_B0010 stem_loop 2941..2960 /label=T7 TE misc_feature 2967..2967 /label=stop polyA_site 2961..2974 /label=polya misc_feature 2934..2974 /label=BBa_B0012 terminator 2934..2974 /label=BBa_B0012 misc_feature 2058..2974 /label=BBa_E0432 stem_loop 2994..3037 /label=stem_loop misc_feature 2983..3062 /label=BBa_B0010 terminator 2983..3062 /label=BBa_B0010 stem_loop 3078..3097 /label=T7 TE misc_feature 3104..3104 /label=stop polyA_site 3098..3111 /label=polya misc_feature 3071..3111 /label=BBa_B0012 terminator 3071..3111 /label=BBa_B0012 terminator 2983..3111 /label=BBa_B0015 promoter 1001..3111 /label=BBa_I12046 misc_feature 3120..3160 /label=B0012 misc_feature 3169..3248 /label=B0010 terminator 3120..3248 /label=BBa_B0025 misc_feature 3265..3281 /label=OR1 misc_feature 3289..3305 /label=OR2 promoter 3304..3309 /label=-35 misc_feature 3315..3321 /label=mutate to obliterate OR3 promoter 3327..3332 /label=-10 promoter 3257..3338 /label=BBa_I12007 misc_feature 3351..3354 /label=conserved RBS 3347..3358 /label=BBa_B0034 CDS 3365..4033 /label=cI 434 promoter 3365..4000 /label=BBa_I12014 stem_loop 4020..4063 /label=stem_loop misc_feature 4009..4088 /label=BBa_B0010 terminator 4009..4088 /label=BBa_B0010 stem_loop 4104..4123 /label=T7 TE misc_feature 4130..4130 /label=stop polyA_site 4124..4137 /label=polya misc_feature 4097..4137 /label=BBa_B0012 terminator 4097..4137 /label=BBa_B0012 terminator 4009..4137 /label=BBa_B0015 misc_feature 3120..4137 /label=BBa_I12002 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgagcacaa aaaagaaacc attaacacaa gagcagcttg 121 aggacgcacg tcgccttaaa gcaatttatg aaaaaaagaa aaatgaactt ggcttatccc 181 aggaatctgt cgcagacaag atggggatgg ggcagtcagg cgttggtgct ttatttaatg 241 gcatcaatgc attaaatgct tataacgccg cattgcttgc aaaaattctc aaagttagcg 301 ttgaagaatt tagcccttca atcgccagag aaatctacga gatgtatgaa gcggttagta 361 tgcagccgtc acttagaagt gagtatgagt accctgtttt ttctcatgtt caggcaggga 421 tgttctcacc tgagcttaga acctttacca aaggtgatgc ggagagatgg gtaagcacaa 481 ccaaaaaagc cagtgattct gcattctggc ttgaggttga aggtaattcc atgaccgcac 541 caacaggctc caagccaagc tttcctgacg gaatgttaat tctcgttgac cctgagcagg 601 ctgttgagcc aggtgatttc tgcatagcca gacttggggg tgatgagttt accttcaaga 661 aactgatcag ggatagcggt caggtgtttt tacaaccact aaacccacag tacccaatga 721 tcccatgcaa tgagagttgt tccgttgtgg ggaaagttat cgctagtcag tggcctgaag 781 agacgtttgg cgctgcaaac gacgaaaact acgctttagt agcttaataa cgctgatagt 841 gctagtgtag atcgctacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga 901 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg 961 ctcaccttcg ggtgggcctt tctgcgttta tatactagag tataaacgca gaaaggccca 1021 cccgaaggtg agccagtgtg actctagtag agagcgttca ccgacaaaca acagataaaa 1081 cgaaaggccc agtctttcga ctgagccttt cgttttattt gatgcctggt actagaggca 1141 accattatca ccgccagagg taaaatagtc aacacgcacg gtgttagata ttacaaactt 1201 tcttgtagat acttacaatg tatcttgtta ctagagaaag aggagaaata ctagatgagc 1261 acaaaaaaga aaccattaac acaagagcag cttgaggacg cacgtcgcct taaagcaatt 1321 tatgaaaaaa agaaaaatga acttggctta tcccaggaat ctgtcgcaga caagatgggg 1381 atggggcagt caggcgttgg tgctttattt aatggcatca atgcattaaa tgcttataac 1441 gccgcattgc ttgcaaaaat tctcaaagtt agcgttgaag aatttagccc ttcaatcgcc 1501 agagaaatct acgagatgta tgaagcggtt agtatgcagc cgtcacttag aagtgagtat 1561 gagtaccctg ttttttctca tgttcaggca gggatgttct cacctgagct tagaaccttt 1621 accaaaggtg atgcggagag atgggtaagc acaaccaaaa aagccagtga ttctgcattc 1681 tggcttgagg ttgaaggtaa ttccatgacc gcaccaacag gctccaagcc aagctttcct 1741 gacggaatgt taattctcgt tgaccctgag caggctgttg agccaggtga tttctgcata 1801 gccagacttg ggggtgatga gtttaccttc aagaaactga tcagggatag cggtcaggtg 1861 tttttacaac cactaaaccc acagtaccca atgatcccat gcaatgagag ttgttccgtt 1921 gtggggaaag ttatcgctag tcagtggcct gaagagacgt ttggcgctgc aaacgacgaa 1981 aactacgctt tagtagctta ataacgctga tagtgctagt gtagatcgct actagagaaa 2041 gaggagaaat actagagaaa gaggagaaat actagatggt gagcaagggc gaggagctgt 2101 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca 2161 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct 2221 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccttc ggctacggcc 2281 tgcaatgctt cgcccgctac cccgaccaca tgaagctgca cgacttcttc aagtccgcca 2341 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga 2401 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca 2461 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc 2521 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc 2581 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca 2641 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagctaccag tccgccctga 2701 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg 2761 ggatcactct cggcatggac gagctgtaca agaggcctgc tgcaaacgac gaaaactacg 2821 ctttagtagc ttaataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa 2881 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact 2941 ggctcacctt cgggtgggcc tttctgcgtt tatatactag agccaggcat caaataaaac 3001 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc 3061 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagagt 3121 ataaacgcag aaaggcccac ccgaaggtga gccagtgtga ctctagtaga gagcgttcac 3181 cgacaaacaa cagataaaac gaaaggccca gtctttcgac tgagcctttc gttttatttg 3241 atgcctggta ctagaggcaa ccattatcac cgccagaggt aaaatagtca acacgcacgg 3301 tgttagatat ttataaatag tggtgataga tttaacgtta ctagagaaag aggagaaata 3361 ctagatgagt atttcttcca gggtaaaaag caaaagaatc cagcttggac ttaaccaggc 3421 tgaacttgct caaaaggtgg ggactaccca gcagtctata gagcagctcg aaaacggtaa 3481 aactaagcga ccacgctttt taccagaact tgcgtcagct cttggcgtaa gtgttgactg 3541 gctgctcaat ggcacctctg attcgaatgt tagatttgtt gggcacgttg agcccaaagg 3601 gaaatatcca ttgattagca tggttagagc tggttcgtgg tgtgaagctt gtgaacccta 3661 cgatatcaag gacattgatg aatggtatga cagtgacgtt aacttattag gcaatggatt 3721 ctggctgaag gttgaaggtg attccatgac ctcacctgta ggtcaaagca tccctgaagg 3781 tcatatggtg ttagtagata ctggacggga gccagtgaat ggaagccttg ttgtagccaa 3841 actgactgac gcgaacgaag caacattcaa gaaactggtc atagatggcg gtcagaagta 3901 cctgaaaggc ctgaatcctt catggcctat gactcctatc aacggaaact gcaagattat 3961 cggtgttgtc gtggaagcga gggtaaaatt cgtataataa tactagagcc aggcatcaaa 4021 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga 4081 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata //