LOCUS       BBa_I12050              2009 bp    DNA     linear       22-Nov-2024
DEFINITION  434 cI (LVA-) Generator (pBAD/araC promoter)
ACCESSION   BBa_I12050
VERSION     BBa_I12050.1  
FEATURES             Location/Qualifiers
     promoter        1080..1210
                     /label=Pbad
     promoter        1043..1114
                     /label=PC
     misc_feature    1..1079
                     /label=AraC
     promoter        1..1210
                     /label=BBa_I0500
     misc_feature    1223..1226
                     /label=conserved
     RBS             1219..1230
                     /label=BBa_B0034
     misc_feature    1867..1872
                     /label=2
     CDS             1237..1872
                     /label=cI 434
     CDS             1237..1872
                     /label=BBa_C0056
     stem_loop       1892..1935
                     /label=stem_loop
     misc_feature    1881..1960
                     /label=BBa_B0010
     terminator      1881..1960
                     /label=BBa_B0010
     stem_loop       1976..1995
                     /label=T7 TE
     misc_feature    2002..2002
                     /label=stop
     polyA_site      1996..2009
                     /label=polya
     misc_feature    1969..2009
                     /label=BBa_B0012
     terminator      1969..2009
                     /label=BBa_B0012
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagaa agaggagaaa tactagatga gtatttcttc cagggtaaaa
     1261 agcaaaagaa tccagcttgg acttaaccag gctgaacttg ctcaaaaggt ggggactacc
     1321 cagcagtcta tagagcagct cgaaaacggt aaaactaagc gaccacgctt tttaccagaa
     1381 cttgcgtcag ctcttggcgt aagtgttgac tggctgctca atggcacctc tgattcgaat
     1441 gttagatttg ttgggcacgt tgagcccaaa gggaaatatc cattgattag catggttaga
     1501 gctggttcgt ggtgtgaagc ttgtgaaccc tacgatatca aggacattga tgaatggtat
     1561 gacagtgacg ttaacttatt aggcaatgga ttctggctga aggttgaagg tgattccatg
     1621 acctcacctg taggtcaaag catccctgaa ggtcatatgg tgttagtaga tactggacgg
     1681 gagccagtga atggaagcct tgttgtagcc aaactgactg acgcgaacga agcaacattc
     1741 aagaaactgg tcatagatgg cggtcagaag tacctgaaag gcctgaatcc ttcatggcct
     1801 atgactccta tcaacggaaa ctgcaagatt atcggtgttg tcgtggaagc gagggtaaaa
     1861 ttcgtataat aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg
     1921 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc
     1981 accttcgggt gggcctttct gcgtttata
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