LOCUS BBa_I12051 4025 bp DNA linear 22-Dec-2024 DEFINITION (New and improved) Test circuit for I12006 ACCESSION BBa_I12051 VERSION BBa_I12051.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 CDS 85..792 /label=cI lambda misc_feature 793..825 /label=LVA misc_feature 832..856 /label=Help:Barcodes CDS 82..856 /label=BBa_C0051 stem_loop 876..919 /label=stem_loop misc_feature 865..944 /label=BBa_B0010 terminator 865..944 /label=BBa_B0010 stem_loop 960..979 /label=T7 TE misc_feature 986..986 /label=stop polyA_site 980..993 /label=polya misc_feature 953..993 /label=BBa_B0012 terminator 953..993 /label=BBa_B0012 terminator 865..993 /label=BBa_B0015 misc_feature 1..993 /label=BBa_I12024 promoter 2081..2211 /label=Pbad promoter 2044..2115 /label=PC misc_feature 1002..2080 /label=AraC promoter 1002..2211 /label=BBa_I0500 misc_feature 2224..2227 /label=conserved RBS 2220..2231 /label=BBa_B0034 misc_feature 2868..2873 /label=2 CDS 2238..2873 /label=cI 434 CDS 2238..2873 /label=BBa_C0056 stem_loop 2893..2936 /label=stem_loop misc_feature 2882..2961 /label=BBa_B0010 terminator 2882..2961 /label=BBa_B0010 stem_loop 2977..2996 /label=T7 TE misc_feature 3003..3003 /label=stop polyA_site 2997..3010 /label=polya misc_feature 2970..3010 /label=BBa_B0012 terminator 2970..3010 /label=BBa_B0012 misc_feature 1002..3010 /label=BBa_I12050 misc_feature 3074..3087 /label=OR1 434 misc_feature 3027..3043 /label=OR1 lambda misc_feature 3051..3067 /label=OR2 lambda promoter 3089..3094 /label=-10 promoter 3066..3071 /label=-35 promoter 3019..3100 /label=BBa_I12006 misc_feature 3113..3116 /label=conserved RBS 3109..3120 /label=BBa_B0034 misc_feature 3883..3888 /label=stop misc_feature 3844..3882 /label=AAV misc_feature 3127..3888 /label=BBa_E0034 stem_loop 3908..3951 /label=stem_loop misc_feature 3897..3976 /label=BBa_B0010 terminator 3897..3976 /label=BBa_B0010 stem_loop 3992..4011 /label=T7 TE misc_feature 4018..4018 /label=stop polyA_site 4012..4025 /label=polya misc_feature 3985..4025 /label=BBa_B0012 terminator 3985..4025 /label=BBa_B0012 misc_feature 3109..4025 /label=BBa_E0434 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagaaagagg agaaatacta gatgagcaca aaaaagaaac cattaacaca agagcagctt 121 gaggacgcac gtcgccttaa agcaatttat gaaaaaaaga aaaatgaact tggcttatcc 181 caggaatctg tcgcagacaa gatggggatg gggcagtcag gcgttggtgc tttatttaat 241 ggcatcaatg cattaaatgc ttataacgcc gcattgcttg caaaaattct caaagttagc 301 gttgaagaat ttagcccttc aatcgccaga gaaatctacg agatgtatga agcggttagt 361 atgcagccgt cacttagaag tgagtatgag taccctgttt tttctcatgt tcaggcaggg 421 atgttctcac ctgagcttag aacctttacc aaaggtgatg cggagagatg ggtaagcaca 481 accaaaaaag ccagtgattc tgcattctgg cttgaggttg aaggtaattc catgaccgca 541 ccaacaggct ccaagccaag ctttcctgac ggaatgttaa ttctcgttga ccctgagcag 601 gctgttgagc caggtgattt ctgcatagcc agacttgggg gtgatgagtt taccttcaag 661 aaactgatca gggatagcgg tcaggtgttt ttacaaccac taaacccaca gtacccaatg 721 atcccatgca atgagagttg ttccgttgtg gggaaagtta tcgctagtca gtggcctgaa 781 gagacgtttg gcgctgcaaa cgacgaaaac tacgctttag tagcttaata acgctgatag 841 tgctagtgta gatcgctact agagccaggc atcaaataaa acgaaaggct cagtcgaaag 901 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg 961 gctcaccttc gggtgggcct ttctgcgttt atatactaga gttatgacaa cttgacggct 1021 acatcattca ctttttcttc acaaccggca cggaactcgc tcgggctggc cccggtgcat 1081 tttttaaata cccgcgagaa atagagttga tcgtcaaaac caacattgcg accgacggtg 1141 gcgataggca tccgggtggt gctcaaaagc agcttcgcct ggctgatacg ttggtcctcg 1201 cgccagctta agacgctaat ccctaactgc tggcggaaaa gatgtgacag acgcgacggc 1261 gacaagcaaa catgctgtgc gacgctggcg atatcaaaat tgctgtctgc caggtgatcg 1321 ctgatgtact gacaagcctc gcgtacccga ttatccatcg gtggatggag cgactcgtta 1381 atcgcttcca tgcgccgcag taacaattgc tcaagcagat ttatcgccag cagctccgaa 1441 tagcgccctt ccccttgccc ggcgttaatg atttgcccaa acaggtcgct gaaatgcggc 1501 tggtgcgctt catccgggcg aaagaacccc gtattggcaa atattgacgg ccagttaagc 1561 cattcatgcc agtaggcgcg cggacgaaag taaacccact ggtgatacca ttcgcgagcc 1621 tccggatgac gaccgtagtg atgaatctct cctggcggga acagcaaaat atcacccggt 1681 cggcaaacaa attctcgtcc ctgatttttc accaccccct gaccgcgaat ggtgagattg 1741 agaatataac ctttcattcc cagcggtcgg tcgataaaaa aatcgagata accgttggcc 1801 tcaatcggcg ttaaacccgc caccagatgg gcattaaacg agtatcccgg cagcagggga 1861 tcattttgcg cttcagccat acttttcata ctcccgccat tcagagaaga aaccaattgt 1921 ccatattgca tcagacattg ccgtcactgc gtcttttact ggctcttctc gctaaccaaa 1981 ccggtaaccc cgcttattaa aagcattctg taacaaagcg ggaccaaagc catgacaaaa 2041 acgcgtaaca aaagtgtcta taatcacggc agaaaagtcc acattgatta tttgcacggc 2101 gtcacacttt gctatgccat agcattttta tccataagat tagcggatcc tacctgacgc 2161 tttttatcgc aactctctac tgtttctcca tacccgtttt tttgggctag ctactagaga 2221 aagaggagaa atactagatg agtatttctt ccagggtaaa aagcaaaaga atccagcttg 2281 gacttaacca ggctgaactt gctcaaaagg tggggactac ccagcagtct atagagcagc 2341 tcgaaaacgg taaaactaag cgaccacgct ttttaccaga acttgcgtca gctcttggcg 2401 taagtgttga ctggctgctc aatggcacct ctgattcgaa tgttagattt gttgggcacg 2461 ttgagcccaa agggaaatat ccattgatta gcatggttag agctggttcg tggtgtgaag 2521 cttgtgaacc ctacgatatc aaggacattg atgaatggta tgacagtgac gttaacttat 2581 taggcaatgg attctggctg aaggttgaag gtgattccat gacctcacct gtaggtcaaa 2641 gcatccctga aggtcatatg gtgttagtag atactggacg ggagccagtg aatggaagcc 2701 ttgttgtagc caaactgact gacgcgaacg aagcaacatt caagaaactg gtcatagatg 2761 gcggtcagaa gtacctgaaa ggcctgaatc cttcatggcc tatgactcct atcaacggaa 2821 actgcaagat tatcggtgtt gtcgtggaag cgagggtaaa attcgtataa taatactaga 2881 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt 2941 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc 3001 tgcgtttata tactagaggc aaccattatc accgccagag gtaaaatagt caacacgcac 3061 ggtgttagat attacaaact ttcttgtata gatttaacgt tactagagaa agaggagaaa 3121 tactagatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag 3181 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc 3241 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg 3301 cccaccctcg tgaccacctt cggctacggc ctgcaatgct tcgcccgcta ccccgaccac 3361 atgaagctgc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc 3421 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac 3481 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg 3541 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag 3601 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag 3661 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac 3721 aaccactacc tgagctacca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac 3781 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac 3841 aagaggcctg ctgcaaacga cgaaaactac gctgctgctg tttaataata ctagagccag 3901 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt 3961 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt 4021 ttata //