##gff-version 3
##sequence-region BBa_I12051 1 4025
BBa_I12051	.	engineered_region	1	993	.	+	0	ID=BBa_I12024;Name=BBa_I12024
BBa_I12051	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_I12024
BBa_I12051	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_I12051	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_I12051	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_I12051	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_I12051	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_I12051	.	CDS	82	856	.	+	0	ID=BBa_C0051;Name=BBa_C0051;Parent=BBa_I12024
BBa_I12051	.	CDS	85	792	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_I12051	.	tag	793	825	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_I12051	.	engineered_tag	832	856	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_I12051	.	terminator	865	993	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_I12024
BBa_I12051	.	terminator	865	944	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I12051	.	stem_loop	876	919	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I12051	.	engineered_region	865	944	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I12051	.	terminator	953	993	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I12051	.	polyA_site	980	993	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I12051	.	stem_loop	960	979	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I12051	.	engineered_region	953	993	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I12051	.	stop_codon	986	986	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I12051	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I12024
BBa_I12051	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I12051	.	engineered_region	1002	3010	.	+	0	ID=BBa_I12050;Name=BBa_I12050
BBa_I12051	.	terminator	2882	2961	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I12050
BBa_I12051	.	stem_loop	2893	2936	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I12051	.	engineered_region	2882	2961	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I12051	.	ribosome_entry_site	2220	2231	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I12050
BBa_I12051	.	conserved_region	2224	2227	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I12051	.	terminator	2970	3010	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I12050
BBa_I12051	.	polyA_site	2997	3010	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I12051	.	stem_loop	2977	2996	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I12051	.	engineered_region	2970	3010	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I12051	.	stop_codon	3003	3003	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I12051	.	CDS	2238	2873	.	+	0	ID=BBa_C0056;Name=BBa_C0056;Parent=BBa_I12050
BBa_I12051	.	CDS	2238	2873	.	+	0	ID=annotation1063931;Name=cI 434;Parent=BBa_C0056
BBa_I12051	.	stop_codon	2868	2873	.	+	0	ID=annotation1063930;Name=2;Parent=BBa_C0056
BBa_I12051	.	promoter	1002	2211	.	+	0	ID=BBa_I0500;Name=BBa_I0500;Parent=BBa_I12050
BBa_I12051	.	promoter	2044	2115	.	+	0	ID=annotation1498842;Name=PC;Parent=BBa_I0500
BBa_I12051	.	promoter	2081	2211	.	+	0	ID=annotation1498841;Name=Pbad;Parent=BBa_I0500
BBa_I12051	.	sequence_feature	1002	2080	.	+	0	ID=annotation1498843;Name=AraC;Parent=BBa_I0500
BBa_I12051	.	promoter	3019	3100	.	+	0	ID=BBa_I12006;Name=BBa_I12006
BBa_I12051	.	non_covalent_binding_site	3027	3043	.	+	0	ID=annotation786500;Name=OR1 lambda;Parent=BBa_I12006
BBa_I12051	.	non_covalent_binding_site	3051	3067	.	+	0	ID=annotation786518;Name=OR2 lambda;Parent=BBa_I12006
BBa_I12051	.	non_covalent_binding_site	3074	3087	.	+	0	ID=annotation786365;Name=OR1 434;Parent=BBa_I12006
BBa_I12051	.	promoter	3066	3071	.	+	0	ID=annotation837284;Name=-35;Parent=BBa_I12006
BBa_I12051	.	promoter	3089	3094	.	+	0	ID=annotation837228;Name=-10;Parent=BBa_I12006
BBa_I12051	.	engineered_region	3109	4025	.	+	0	ID=BBa_E0434;Name=BBa_E0434
BBa_I12051	.	engineered_region	3127	3888	.	+	0	ID=BBa_E0034;Name=BBa_E0034;Parent=BBa_E0434
BBa_I12051	.	stop_codon	3883	3888	.	+	0	ID=annotation1245671;Name=stop;Parent=BBa_E0034
BBa_I12051	.	tag	3844	3882	.	+	0	ID=annotation1245672;Name=AAV;Parent=BBa_E0034
BBa_I12051	.	terminator	3897	3976	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0434
BBa_I12051	.	stem_loop	3908	3951	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I12051	.	engineered_region	3897	3976	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I12051	.	ribosome_entry_site	3109	3120	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0434
BBa_I12051	.	conserved_region	3113	3116	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I12051	.	terminator	3985	4025	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0434
BBa_I12051	.	polyA_site	4012	4025	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I12051	.	stem_loop	3992	4011	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I12051	.	engineered_region	3985	4025	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I12051	.	stop_codon	4018	4018	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
>BBa_I12051
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatact
agatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaa
gaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttattt
aatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcc
cttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccc
tgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggta
agcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctcca
agccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccag
acttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacag
tacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttg
gcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccagg
catcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctact
agagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttatgacaacttgacggctacatcat
tcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagag
ttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctg
atacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgaca
agcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcg
tacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagattt
atcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggct
ggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcg
cggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcggg
aacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagat
tgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacc
cgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccg
ccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaa
ccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaag
tgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatcc
ataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagct
actagagaaagaggagaaatactagatgagtatttcttccagggtaaaaagcaaaagaatccagcttggacttaaccag
gctgaacttgctcaaaaggtggggactacccagcagtctatagagcagctcgaaaacggtaaaactaagcgaccacgct
ttttaccagaacttgcgtcagctcttggcgtaagtgttgactggctgctcaatggcacctctgattcgaatgttagatt
tgttgggcacgttgagcccaaagggaaatatccattgattagcatggttagagctggttcgtggtgtgaagcttgtgaa
ccctacgatatcaaggacattgatgaatggtatgacagtgacgttaacttattaggcaatggattctggctgaaggttg
aaggtgattccatgacctcacctgtaggtcaaagcatccctgaaggtcatatggtgttagtagatactggacgggagcc
agtgaatggaagccttgttgtagccaaactgactgacgcgaacgaagcaacattcaagaaactggtcatagatggcggt
cagaagtacctgaaaggcctgaatccttcatggcctatgactcctatcaacggaaactgcaagattatcggtgttgtcg
tggaagcgagggtaaaattcgtataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactggg
cctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctg
cgtttatatactagaggcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttagatattacaaactt
tcttgtatagatttaacgttactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccgggg
tggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgc
cacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccacc
ttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccg
aaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgaggg
cgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggag
tacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgcc
acaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgct
gcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctg
gagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacg
ctgctgctgtttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtttta
tctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata