LOCUS BBa_I12058 3925 bp DNA linear 18-May-2024 DEFINITION BL oscillator (I12006-based) with IPTG kickstart ACCESSION BBa_I12058 VERSION BBa_I12058.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 CDS 85..792 /label=cI lambda misc_feature 793..825 /label=LVA misc_feature 832..856 /label=Help:Barcodes CDS 82..856 /label=BBa_C0051 stem_loop 876..919 /label=stem_loop misc_feature 865..944 /label=BBa_B0010 terminator 865..944 /label=BBa_B0010 stem_loop 960..979 /label=T7 TE misc_feature 986..986 /label=stop polyA_site 980..993 /label=polya misc_feature 953..993 /label=BBa_B0012 terminator 953..993 /label=BBa_B0012 terminator 865..993 /label=BBa_B0015 misc_feature 1..993 /label=BBa_I12024 misc_feature 1010..1026 /label=OR1 misc_feature 1034..1050 /label=OR2 promoter 1049..1054 /label=-35 misc_feature 1060..1066 /label=mutate to obliterate OR3 promoter 1072..1077 /label=-10 promoter 1002..1083 /label=BBa_I12007 misc_feature 1096..1099 /label=conserved RBS 1092..1103 /label=BBa_B0034 misc_feature 1740..1745 /label=2 CDS 1110..1745 /label=cI 434 CDS 1110..1745 /label=BBa_C0056 stem_loop 1765..1808 /label=stem_loop misc_feature 1754..1833 /label=BBa_B0010 terminator 1754..1833 /label=BBa_B0010 stem_loop 1849..1868 /label=T7 TE misc_feature 1875..1875 /label=stop polyA_site 1869..1882 /label=polya misc_feature 1842..1882 /label=BBa_B0012 terminator 1842..1882 /label=BBa_B0012 misc_feature 1002..1882 /label=BBa_I12054 misc_feature 1946..1959 /label=OR1 434 misc_feature 1899..1915 /label=OR1 lambda misc_feature 1923..1939 /label=OR2 lambda promoter 1961..1966 /label=-10 promoter 1938..1943 /label=-35 promoter 1891..1972 /label=BBa_I12006 misc_feature 1985..1988 /label=conserved RBS 1981..1992 /label=BBa_B0034 CDS 2002..2709 /label=cI lambda misc_feature 2710..2742 /label=LVA misc_feature 2749..2773 /label=Help:Barcodes CDS 1999..2748 /label=BBa_C0051 stem_loop 2793..2836 /label=stem_loop misc_feature 2782..2861 /label=BBa_B0010 terminator 2782..2861 /label=BBa_B0010 stem_loop 2877..2896 /label=T7 TE misc_feature 2903..2903 /label=stop polyA_site 2897..2910 /label=polya misc_feature 2870..2910 /label=BBa_B0012 terminator 2870..2910 /label=BBa_B0012 misc_feature 2974..2987 /label=OR1 434 misc_feature 2927..2943 /label=OR1 lambda misc_feature 2951..2967 /label=OR2 lambda promoter 2989..2994 /label=-10 promoter 2966..2971 /label=-35 promoter 2919..3000 /label=BBa_I12006 misc_feature 1891..3000 /label=BBa_I12055 misc_feature 3013..3016 /label=conserved RBS 3009..3020 /label=BBa_B0034 misc_feature 3783..3788 /label=stop misc_feature 3744..3782 /label=AAV misc_feature 3027..3788 /label=BBa_E0034 stem_loop 3808..3851 /label=stem_loop misc_feature 3797..3876 /label=BBa_B0010 terminator 3797..3876 /label=BBa_B0010 stem_loop 3892..3911 /label=T7 TE misc_feature 3918..3918 /label=stop polyA_site 3912..3925 /label=polya misc_feature 3885..3925 /label=BBa_B0012 terminator 3885..3925 /label=BBa_B0012 misc_feature 3009..3925 /label=BBa_E0434 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagaaagagg agaaatacta gatgagcaca aaaaagaaac cattaacaca agagcagctt 121 gaggacgcac gtcgccttaa agcaatttat gaaaaaaaga aaaatgaact tggcttatcc 181 caggaatctg tcgcagacaa gatggggatg gggcagtcag gcgttggtgc tttatttaat 241 ggcatcaatg cattaaatgc ttataacgcc gcattgcttg caaaaattct caaagttagc 301 gttgaagaat ttagcccttc aatcgccaga gaaatctacg agatgtatga agcggttagt 361 atgcagccgt cacttagaag tgagtatgag taccctgttt tttctcatgt tcaggcaggg 421 atgttctcac ctgagcttag aacctttacc aaaggtgatg cggagagatg ggtaagcaca 481 accaaaaaag ccagtgattc tgcattctgg cttgaggttg aaggtaattc catgaccgca 541 ccaacaggct ccaagccaag ctttcctgac ggaatgttaa ttctcgttga ccctgagcag 601 gctgttgagc caggtgattt ctgcatagcc agacttgggg gtgatgagtt taccttcaag 661 aaactgatca gggatagcgg tcaggtgttt ttacaaccac taaacccaca gtacccaatg 721 atcccatgca atgagagttg ttccgttgtg gggaaagtta tcgctagtca gtggcctgaa 781 gagacgtttg gcgctgcaaa cgacgaaaac tacgctttag tagcttaata acgctgatag 841 tgctagtgta gatcgctact agagccaggc atcaaataaa acgaaaggct cagtcgaaag 901 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg 961 gctcaccttc gggtgggcct ttctgcgttt atatactaga ggcaaccatt atcaccgcca 1021 gaggtaaaat agtcaacacg cacggtgtta gatatttata aatagtggtg atagatttaa 1081 cgttactaga gaaagaggag aaatactaga tgagtatttc ttccagggta aaaagcaaaa 1141 gaatccagct tggacttaac caggctgaac ttgctcaaaa ggtggggact acccagcagt 1201 ctatagagca gctcgaaaac ggtaaaacta agcgaccacg ctttttacca gaacttgcgt 1261 cagctcttgg cgtaagtgtt gactggctgc tcaatggcac ctctgattcg aatgttagat 1321 ttgttgggca cgttgagccc aaagggaaat atccattgat tagcatggtt agagctggtt 1381 cgtggtgtga agcttgtgaa ccctacgata tcaaggacat tgatgaatgg tatgacagtg 1441 acgttaactt attaggcaat ggattctggc tgaaggttga aggtgattcc atgacctcac 1501 ctgtaggtca aagcatccct gaaggtcata tggtgttagt agatactgga cgggagccag 1561 tgaatggaag ccttgttgta gccaaactga ctgacgcgaa cgaagcaaca ttcaagaaac 1621 tggtcataga tggcggtcag aagtacctga aaggcctgaa tccttcatgg cctatgactc 1681 ctatcaacgg aaactgcaag attatcggtg ttgtcgtgga agcgagggta aaattcgtat 1741 aataatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt 1801 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg 1861 ggtgggcctt tctgcgttta tatactagag gcaaccatta tcaccgccag aggtaaaata 1921 gtcaacacgc acggtgttag atattacaaa ctttcttgta tagatttaac gttactagag 1981 aaagaggaga aatactagat gagcacaaaa aagaaaccat taacacaaga gcagcttgag 2041 gacgcacgtc gccttaaagc aatttatgaa aaaaagaaaa atgaacttgg cttatcccag 2101 gaatctgtcg cagacaagat ggggatgggg cagtcaggcg ttggtgcttt atttaatggc 2161 atcaatgcat taaatgctta taacgccgca ttgcttgcaa aaattctcaa agttagcgtt 2221 gaagaattta gcccttcaat cgccagagaa atctacgaga tgtatgaagc ggttagtatg 2281 cagccgtcac ttagaagtga gtatgagtac cctgtttttt ctcatgttca ggcagggatg 2341 ttctcacctg agcttagaac ctttaccaaa ggtgatgcgg agagatgggt aagcacaacc 2401 aaaaaagcca gtgattctgc attctggctt gaggttgaag gtaattccat gaccgcacca 2461 acaggctcca agccaagctt tcctgacgga atgttaattc tcgttgaccc tgagcaggct 2521 gttgagccag gtgatttctg catagccaga cttgggggtg atgagtttac cttcaagaaa 2581 ctgatcaggg atagcggtca ggtgttttta caaccactaa acccacagta cccaatgatc 2641 ccatgcaatg agagttgttc cgttgtgggg aaagttatcg ctagtcagtg gcctgaagag 2701 acgtttggcg ctgcaaacga cgaaaactac gctttagtag cttaataacg ctgatagtgc 2761 tagtgtagat cgctactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact 2821 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct 2881 caccttcggg tgggcctttc tgcgtttata tactagaggc aaccattatc accgccagag 2941 gtaaaatagt caacacgcac ggtgttagat attacaaact ttcttgtata gatttaacgt 3001 tactagagaa agaggagaaa tactagatgg tgagcaaggg cgaggagctg ttcaccgggg 3061 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg 3121 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg 3181 gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt cggctacggc ctgcaatgct 3241 tcgcccgcta ccccgaccac atgaagctgc acgacttctt caagtccgcc atgcccgaag 3301 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg 3361 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca 3421 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct 3481 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca 3541 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg 3601 gccccgtgct gctgcccgac aaccactacc tgagctacca gtccgccctg agcaaagacc 3661 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc 3721 tcggcatgga cgagctgtac aagaggcctg ctgcaaacga cgaaaactac gctgctgctg 3781 tttaataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc 3841 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct 3901 tcgggtgggc ctttctgcgt ttata //