LOCUS BBa_I12059 3943 bp DNA linear 18-May-2024 DEFINITION BL oscillator (I12036 based) with IPTG kickstart ACCESSION BBa_I12059 VERSION BBa_I12059.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 CDS 85..792 /label=cI lambda misc_feature 793..825 /label=LVA misc_feature 832..856 /label=Help:Barcodes CDS 82..856 /label=BBa_C0051 stem_loop 876..919 /label=stem_loop misc_feature 865..944 /label=BBa_B0010 terminator 865..944 /label=BBa_B0010 stem_loop 960..979 /label=T7 TE misc_feature 986..986 /label=stop polyA_site 980..993 /label=polya misc_feature 953..993 /label=BBa_B0012 terminator 953..993 /label=BBa_B0012 terminator 865..993 /label=BBa_B0015 misc_feature 1..993 /label=BBa_I12024 misc_feature 1010..1026 /label=OR1 misc_feature 1034..1050 /label=OR2 promoter 1049..1054 /label=-35 misc_feature 1060..1066 /label=mutate to obliterate OR3 promoter 1072..1077 /label=-10 promoter 1002..1083 /label=BBa_I12007 misc_feature 1096..1099 /label=conserved RBS 1092..1103 /label=BBa_B0034 misc_feature 1740..1745 /label=2 CDS 1110..1745 /label=cI 434 CDS 1110..1745 /label=BBa_C0056 stem_loop 1765..1808 /label=stem_loop misc_feature 1754..1833 /label=BBa_B0010 terminator 1754..1833 /label=BBa_B0010 stem_loop 1849..1868 /label=T7 TE misc_feature 1875..1875 /label=stop polyA_site 1869..1882 /label=polya misc_feature 1842..1882 /label=BBa_B0012 terminator 1842..1882 /label=BBa_B0012 misc_feature 1002..1882 /label=BBa_I12054 misc_feature 1899..1915 /label=OR1 lambda misc_feature 1923..1939 /label=OR2 lambda misc_feature 1946..1959 /label=OR1 434 misc_feature 1968..1981 /label=OR2 434 promoter 1938..1943 /label=-35 promoter 1961..1966 /label=-10 misc_feature 1973..1973 /label=start promoter 1891..1981 /label=BBa_I12036 misc_feature 1994..1997 /label=conserved RBS 1990..2001 /label=BBa_B0034 CDS 2011..2718 /label=cI lambda misc_feature 2719..2751 /label=LVA misc_feature 2758..2782 /label=Help:Barcodes CDS 2008..2757 /label=BBa_C0051 stem_loop 2802..2845 /label=stem_loop misc_feature 2791..2870 /label=BBa_B0010 terminator 2791..2870 /label=BBa_B0010 stem_loop 2886..2905 /label=T7 TE misc_feature 2912..2912 /label=stop polyA_site 2906..2919 /label=polya misc_feature 2879..2919 /label=BBa_B0012 terminator 2879..2919 /label=BBa_B0012 misc_feature 2936..2952 /label=OR1 lambda misc_feature 2960..2976 /label=OR2 lambda misc_feature 2983..2996 /label=OR1 434 misc_feature 3005..3018 /label=OR2 434 promoter 2975..2980 /label=-35 promoter 2998..3003 /label=-10 misc_feature 3010..3010 /label=start promoter 2928..3018 /label=BBa_I12036 misc_feature 1891..3018 /label=BBa_I12056 misc_feature 3031..3034 /label=conserved RBS 3027..3038 /label=BBa_B0034 misc_feature 3801..3806 /label=stop misc_feature 3762..3800 /label=AAV misc_feature 3045..3806 /label=BBa_E0034 stem_loop 3826..3869 /label=stem_loop misc_feature 3815..3894 /label=BBa_B0010 terminator 3815..3894 /label=BBa_B0010 stem_loop 3910..3929 /label=T7 TE misc_feature 3936..3936 /label=stop polyA_site 3930..3943 /label=polya misc_feature 3903..3943 /label=BBa_B0012 terminator 3903..3943 /label=BBa_B0012 misc_feature 3027..3943 /label=BBa_E0434 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagaaagagg agaaatacta gatgagcaca aaaaagaaac cattaacaca agagcagctt 121 gaggacgcac gtcgccttaa agcaatttat gaaaaaaaga aaaatgaact tggcttatcc 181 caggaatctg tcgcagacaa gatggggatg gggcagtcag gcgttggtgc tttatttaat 241 ggcatcaatg cattaaatgc ttataacgcc gcattgcttg caaaaattct caaagttagc 301 gttgaagaat ttagcccttc aatcgccaga gaaatctacg agatgtatga agcggttagt 361 atgcagccgt cacttagaag tgagtatgag taccctgttt tttctcatgt tcaggcaggg 421 atgttctcac ctgagcttag aacctttacc aaaggtgatg cggagagatg ggtaagcaca 481 accaaaaaag ccagtgattc tgcattctgg cttgaggttg aaggtaattc catgaccgca 541 ccaacaggct ccaagccaag ctttcctgac ggaatgttaa ttctcgttga ccctgagcag 601 gctgttgagc caggtgattt ctgcatagcc agacttgggg gtgatgagtt taccttcaag 661 aaactgatca gggatagcgg tcaggtgttt ttacaaccac taaacccaca gtacccaatg 721 atcccatgca atgagagttg ttccgttgtg gggaaagtta tcgctagtca gtggcctgaa 781 gagacgtttg gcgctgcaaa cgacgaaaac tacgctttag tagcttaata acgctgatag 841 tgctagtgta gatcgctact agagccaggc atcaaataaa acgaaaggct cagtcgaaag 901 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg 961 gctcaccttc gggtgggcct ttctgcgttt atatactaga ggcaaccatt atcaccgcca 1021 gaggtaaaat agtcaacacg cacggtgtta gatatttata aatagtggtg atagatttaa 1081 cgttactaga gaaagaggag aaatactaga tgagtatttc ttccagggta aaaagcaaaa 1141 gaatccagct tggacttaac caggctgaac ttgctcaaaa ggtggggact acccagcagt 1201 ctatagagca gctcgaaaac ggtaaaacta agcgaccacg ctttttacca gaacttgcgt 1261 cagctcttgg cgtaagtgtt gactggctgc tcaatggcac ctctgattcg aatgttagat 1321 ttgttgggca cgttgagccc aaagggaaat atccattgat tagcatggtt agagctggtt 1381 cgtggtgtga agcttgtgaa ccctacgata tcaaggacat tgatgaatgg tatgacagtg 1441 acgttaactt attaggcaat ggattctggc tgaaggttga aggtgattcc atgacctcac 1501 ctgtaggtca aagcatccct gaaggtcata tggtgttagt agatactgga cgggagccag 1561 tgaatggaag ccttgttgta gccaaactga ctgacgcgaa cgaagcaaca ttcaagaaac 1621 tggtcataga tggcggtcag aagtacctga aaggcctgaa tccttcatgg cctatgactc 1681 ctatcaacgg aaactgcaag attatcggtg ttgtcgtgga agcgagggta aaattcgtat 1741 aataatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt 1801 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg 1861 ggtgggcctt tctgcgttta tatactagag gcaaccatta tcaccgccag aggtaaaata 1921 gtcaacacgc acggtgttag atattacaaa ctttcttgta tagatttaca atgtatcttg 1981 ttactagaga aagaggagaa atactagatg agcacaaaaa agaaaccatt aacacaagag 2041 cagcttgagg acgcacgtcg ccttaaagca atttatgaaa aaaagaaaaa tgaacttggc 2101 ttatcccagg aatctgtcgc agacaagatg gggatggggc agtcaggcgt tggtgcttta 2161 tttaatggca tcaatgcatt aaatgcttat aacgccgcat tgcttgcaaa aattctcaaa 2221 gttagcgttg aagaatttag cccttcaatc gccagagaaa tctacgagat gtatgaagcg 2281 gttagtatgc agccgtcact tagaagtgag tatgagtacc ctgttttttc tcatgttcag 2341 gcagggatgt tctcacctga gcttagaacc tttaccaaag gtgatgcgga gagatgggta 2401 agcacaacca aaaaagccag tgattctgca ttctggcttg aggttgaagg taattccatg 2461 accgcaccaa caggctccaa gccaagcttt cctgacggaa tgttaattct cgttgaccct 2521 gagcaggctg ttgagccagg tgatttctgc atagccagac ttgggggtga tgagtttacc 2581 ttcaagaaac tgatcaggga tagcggtcag gtgtttttac aaccactaaa cccacagtac 2641 ccaatgatcc catgcaatga gagttgttcc gttgtgggga aagttatcgc tagtcagtgg 2701 cctgaagaga cgtttggcgc tgcaaacgac gaaaactacg ctttagtagc ttaataacgc 2761 tgatagtgct agtgtagatc gctactagag ccaggcatca aataaaacga aaggctcagt 2821 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc 2881 acactggctc accttcgggt gggcctttct gcgtttatat actagaggca accattatca 2941 ccgccagagg taaaatagtc aacacgcacg gtgttagata ttacaaactt tcttgtatag 3001 atttacaatg tatcttgtta ctagagaaag aggagaaata ctagatggtg agcaagggcg 3061 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc 3121 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga 3181 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccttcg 3241 gctacggcct gcaatgcttc gcccgctacc ccgaccacat gaagctgcac gacttcttca 3301 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca 3361 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc 3421 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact 3481 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact 3541 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga 3601 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agctaccagt 3661 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga 3721 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gaggcctgct gcaaacgacg 3781 aaaactacgc tgctgctgtt taataatact agagccaggc atcaaataaa acgaaaggct 3841 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag 3901 agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata //