##gff-version 3
##sequence-region BBa_I12059 1 3943
BBa_I12059	.	engineered_region	1891	3018	.	+	0	ID=BBa_I12056;Name=BBa_I12056
BBa_I12059	.	promoter	2928	3018	.	+	0	ID=BBa_I12036;Name=BBa_I12036;Parent=BBa_I12056
BBa_I12059	.	start_codon	3010	3010	.	+	0	ID=annotation1062203;Name=start;Parent=BBa_I12036
BBa_I12059	.	promoter	2975	2980	.	+	0	ID=annotation1056739;Name=-35;Parent=BBa_I12036
BBa_I12059	.	promoter	2998	3003	.	+	0	ID=annotation1056740;Name=-10;Parent=BBa_I12036
BBa_I12059	.	non_covalent_binding_site	2936	2952	.	+	0	ID=annotation1056735;Name=OR1 lambda;Parent=BBa_I12036
BBa_I12059	.	non_covalent_binding_site	3005	3018	.	+	0	ID=annotation1056738;Name=OR2 434;Parent=BBa_I12036
BBa_I12059	.	non_covalent_binding_site	2960	2976	.	+	0	ID=annotation1056736;Name=OR2 lambda;Parent=BBa_I12036
BBa_I12059	.	non_covalent_binding_site	2983	2996	.	+	0	ID=annotation1056737;Name=OR1 434;Parent=BBa_I12036
BBa_I12059	.	ribosome_entry_site	1990	2001	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I12056
BBa_I12059	.	conserved_region	1994	1997	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I12059	.	terminator	2879	2919	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I12056
BBa_I12059	.	stem_loop	2886	2905	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I12059	.	engineered_region	2879	2919	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I12059	.	stop_codon	2912	2912	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I12059	.	polyA_site	2906	2919	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I12059	.	promoter	1891	1981	.	+	0	ID=BBa_I12036;Name=BBa_I12036;Parent=BBa_I12056
BBa_I12059	.	start_codon	1973	1973	.	+	0	ID=annotation1062203;Name=start;Parent=BBa_I12036
BBa_I12059	.	promoter	1938	1943	.	+	0	ID=annotation1056739;Name=-35;Parent=BBa_I12036
BBa_I12059	.	promoter	1961	1966	.	+	0	ID=annotation1056740;Name=-10;Parent=BBa_I12036
BBa_I12059	.	non_covalent_binding_site	1899	1915	.	+	0	ID=annotation1056735;Name=OR1 lambda;Parent=BBa_I12036
BBa_I12059	.	non_covalent_binding_site	1968	1981	.	+	0	ID=annotation1056738;Name=OR2 434;Parent=BBa_I12036
BBa_I12059	.	non_covalent_binding_site	1923	1939	.	+	0	ID=annotation1056736;Name=OR2 lambda;Parent=BBa_I12036
BBa_I12059	.	non_covalent_binding_site	1946	1959	.	+	0	ID=annotation1056737;Name=OR1 434;Parent=BBa_I12036
BBa_I12059	.	terminator	2791	2870	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I12056
BBa_I12059	.	engineered_region	2791	2870	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I12059	.	stem_loop	2802	2845	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I12059	.	CDS	2008	2757	.	+	0	ID=BBa_C0051;Name=BBa_C0051;Parent=BBa_I12056
BBa_I12059	.	engineered_tag	2758	2782	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_I12059	.	CDS	2011	2718	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_I12059	.	tag	2719	2751	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_I12059	.	engineered_region	3027	3943	.	+	0	ID=BBa_E0434;Name=BBa_E0434
BBa_I12059	.	terminator	3903	3943	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0434
BBa_I12059	.	stem_loop	3910	3929	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I12059	.	engineered_region	3903	3943	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I12059	.	stop_codon	3936	3936	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I12059	.	polyA_site	3930	3943	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I12059	.	terminator	3815	3894	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0434
BBa_I12059	.	engineered_region	3815	3894	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I12059	.	stem_loop	3826	3869	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I12059	.	ribosome_entry_site	3027	3038	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0434
BBa_I12059	.	conserved_region	3031	3034	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I12059	.	engineered_region	3045	3806	.	+	0	ID=BBa_E0034;Name=BBa_E0034;Parent=BBa_E0434
BBa_I12059	.	stop_codon	3801	3806	.	+	0	ID=annotation1245671;Name=stop;Parent=BBa_E0034
BBa_I12059	.	tag	3762	3800	.	+	0	ID=annotation1245672;Name=AAV;Parent=BBa_E0034
BBa_I12059	.	engineered_region	1002	1882	.	+	0	ID=BBa_I12054;Name=BBa_I12054
BBa_I12059	.	terminator	1842	1882	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I12054
BBa_I12059	.	stem_loop	1849	1868	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I12059	.	engineered_region	1842	1882	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I12059	.	stop_codon	1875	1875	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I12059	.	polyA_site	1869	1882	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I12059	.	ribosome_entry_site	1092	1103	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I12054
BBa_I12059	.	conserved_region	1096	1099	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I12059	.	CDS	1110	1745	.	+	0	ID=BBa_C0056;Name=BBa_C0056;Parent=BBa_I12054
BBa_I12059	.	stop_codon	1740	1745	.	+	0	ID=annotation1063930;Name=2;Parent=BBa_C0056
BBa_I12059	.	CDS	1110	1745	.	+	0	ID=annotation1063931;Name=cI 434;Parent=BBa_C0056
BBa_I12059	.	promoter	1002	1083	.	+	0	ID=BBa_I12007;Name=BBa_I12007;Parent=BBa_I12054
BBa_I12059	.	promoter	1049	1054	.	+	0	ID=annotation937703;Name=-35;Parent=BBa_I12007
BBa_I12059	.	non_covalent_binding_site	1034	1050	.	+	0	ID=annotation937702;Name=OR2;Parent=BBa_I12007
BBa_I12059	.	non_covalent_binding_site	1010	1026	.	+	0	ID=annotation937701;Name=OR1;Parent=BBa_I12007
BBa_I12059	.	sequence_alteration	1060	1066	.	+	0	ID=annotation937704;Name=mutate to obliterate OR3;Parent=BBa_I12007
BBa_I12059	.	promoter	1072	1077	.	+	0	ID=annotation937705;Name=-10;Parent=BBa_I12007
BBa_I12059	.	terminator	1754	1833	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I12054
BBa_I12059	.	engineered_region	1754	1833	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I12059	.	stem_loop	1765	1808	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I12059	.	engineered_region	1	993	.	+	0	ID=BBa_I12024;Name=BBa_I12024
BBa_I12059	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_I12024
BBa_I12059	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_I12059	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_I12059	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_I12059	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_I12059	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_I12059	.	CDS	82	856	.	+	0	ID=BBa_C0051;Name=BBa_C0051;Parent=BBa_I12024
BBa_I12059	.	engineered_tag	832	856	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_I12059	.	CDS	85	792	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_I12059	.	tag	793	825	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_I12059	.	terminator	865	993	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_I12024
BBa_I12059	.	terminator	953	993	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I12059	.	stem_loop	960	979	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I12059	.	engineered_region	953	993	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I12059	.	stop_codon	986	986	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I12059	.	polyA_site	980	993	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I12059	.	terminator	865	944	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I12059	.	engineered_region	865	944	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I12059	.	stem_loop	876	919	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I12059	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I12024
BBa_I12059	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_I12059
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatact
agatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaa
gaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttattt
aatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcc
cttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccc
tgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggta
agcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctcca
agccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccag
acttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacag
tacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttg
gcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccagg
catcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctact
agagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggcaaccattatcaccgccagaggtaa
aatagtcaacacgcacggtgttagatatttataaatagtggtgatagatttaacgttactagagaaagaggagaaatac
tagatgagtatttcttccagggtaaaaagcaaaagaatccagcttggacttaaccaggctgaacttgctcaaaaggtgg
ggactacccagcagtctatagagcagctcgaaaacggtaaaactaagcgaccacgctttttaccagaacttgcgtcagc
tcttggcgtaagtgttgactggctgctcaatggcacctctgattcgaatgttagatttgttgggcacgttgagcccaaa
gggaaatatccattgattagcatggttagagctggttcgtggtgtgaagcttgtgaaccctacgatatcaaggacattg
atgaatggtatgacagtgacgttaacttattaggcaatggattctggctgaaggttgaaggtgattccatgacctcacc
tgtaggtcaaagcatccctgaaggtcatatggtgttagtagatactggacgggagccagtgaatggaagccttgttgta
gccaaactgactgacgcgaacgaagcaacattcaagaaactggtcatagatggcggtcagaagtacctgaaaggcctga
atccttcatggcctatgactcctatcaacggaaactgcaagattatcggtgttgtcgtggaagcgagggtaaaattcgt
ataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgttt
gtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggcaacc
attatcaccgccagaggtaaaatagtcaacacgcacggtgttagatattacaaactttcttgtatagatttacaatgta
tcttgttactagagaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgc
acgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatgggg
atggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaa
ttctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgca
gccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacc
tttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaag
gtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggc
tgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggt
caggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagtta
tcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgata
gtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtttta
tctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatact
agaggcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttagatattacaaactttcttgtatagat
ttacaatgtatcttgttactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtgg
tgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccac
ctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttc
ggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaag
gctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcga
caccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtac
aactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccaca
acatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcc
cgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggag
ttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctg
ctgctgtttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatct
gttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata