BBa_I12059
type: ComponentDefinition
persistent identity: BBa I12059
display id: BBa_I12059
version: 1
was generated by: iGEM to SBOL conversion
description: BL oscillator (I12036 based) with IPTG kickstart
created: 2004-09-02T11:00:00Z
modified: 2015-08-31T04:07:31Z
owned by: james
owned by: myers
mutable description: Modified BL oscillator based on I12036. It can be "reset" by adding IPTG which stimulates production of the A protein (lambda cI) if used in LacIq strain. The B protein is 434 cI. The YFP-AAV reporter tracks the dynamics of the A protein.
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 145
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: endelman
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_I12054
component: BBa_I12024
component: BBa_E0434
component: BBa_I12056
sequence annotation: BBa_I12024
sequence annotation: BBa_I12054
sequence annotation: BBa_I12056
sequence annotation: BBa_E0434
sequence: https://synbiohub.org/public/igem/BBa_I12059_sequence/1

BBa_I12054
type: Component
persistent identity: component2237703
display id: component2237703
version: 1
top level: BBa_I12059
definition: BBa_I12054
access: public

BBa_I12024
type: Component
persistent identity: component2237685
display id: component2237685
version: 1
top level: BBa_I12059
definition: BBa_I12024
access: public

BBa_E0434
type: Component
persistent identity: component2237742
display id: component2237742
version: 1
top level: BBa_I12059
definition: BBa_E0434
access: public

BBa_I12056
type: Component
persistent identity: component2237732
display id: component2237732
version: 1
top level: BBa_I12059
definition: BBa_I12056
access: public

BBa_I12024
type: SequenceAnnotation
persistent identity: annotation2237685
display id: annotation2237685
version: 1
top level: BBa_I12059
location: https://synbiohub.org/public/igem/BBa_I12059/annotation2237685/range2237685/1
component: BBa_I12024

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type: Range
persistent identity: range2237685
display id: range2237685
version: 1
top level: BBa_I12059
direction: direction/reverse
start: 1
end: 993
orientation: inline

BBa_I12054
type: SequenceAnnotation
persistent identity: annotation2237703
display id: annotation2237703
version: 1
top level: BBa_I12059
location: https://synbiohub.org/public/igem/BBa_I12059/annotation2237703/range2237703/1
component: BBa_I12054

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type: Range
persistent identity: range2237703
display id: range2237703
version: 1
top level: BBa_I12059
direction: direction/reverse
start: 1002
end: 1882
orientation: inline

BBa_I12056
type: SequenceAnnotation
persistent identity: annotation2237732
display id: annotation2237732
version: 1
top level: BBa_I12059
location: https://synbiohub.org/public/igem/BBa_I12059/annotation2237732/range2237732/1
component: BBa_I12056

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type: Range
persistent identity: range2237732
display id: range2237732
version: 1
top level: BBa_I12059
direction: direction/reverse
start: 1891
end: 3018
orientation: inline

BBa_E0434
type: SequenceAnnotation
persistent identity: annotation2237742
display id: annotation2237742
version: 1
top level: BBa_I12059
location: https://synbiohub.org/public/igem/BBa_I12059/annotation2237742/range2237742/1
component: BBa_E0434

https://synbiohub.org/public/igem/BBa_I12059/annotation2237742/range2237742/1
type: Range
persistent identity: range2237742
display id: range2237742
version: 1
top level: BBa_I12059
direction: direction/reverse
start: 3027
end: 3943
orientation: inline

BBa_I12024
type: ComponentDefinition
persistent identity: BBa I12024
display id: BBa_I12024
version: 1
was generated by: iGEM to SBOL conversion
description: Lambda cI Generator (PLlac 0-1 promoter )
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:31Z
owned by: james
owned by: myers
mutable description: Expression of Lambda cI (LVA+) can be controlled by IPTG in LacIq strain.
discontinued: false
dominant: true
experience: experience/None
group u list: _3_
m user id: 0
owner id: 145
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: endelman
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0015
component: BBa_C0051
component: BBa_B0034
component: BBa_R0011
sequence annotation: BBa_R0011
sequence annotation: BBa_B0034
sequence annotation: BBa_C0051
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_I12024_sequence/1

BBa_B0015
type: Component
persistent identity: component2221570
display id: component2221570
version: 1
top level: BBa_I12024
definition: BBa_B0015
access: public

BBa_C0051
type: Component
persistent identity: component2221563
display id: component2221563
version: 1
top level: BBa_I12024
definition: cI lam
access: public

BBa_B0034
type: Component
persistent identity: component2221559
display id: component2221559
version: 1
top level: BBa_I12024
definition: BBa_B0034
access: public

BBa_R0011
type: Component
persistent identity: component2221553
display id: component2221553
version: 1
top level: BBa_I12024
definition: lacI+pL
access: public

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2221553
display id: annotation2221553
version: 1
top level: BBa_I12024
location: https://synbiohub.org/public/igem/BBa_I12024/annotation2221553/range2221553/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_I12024/annotation2221553/range2221553/1
type: Range
persistent identity: range2221553
display id: range2221553
version: 1
top level: BBa_I12024
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2221559
display id: annotation2221559
version: 1
top level: BBa_I12024
location: https://synbiohub.org/public/igem/BBa_I12024/annotation2221559/range2221559/1
component: BBa_B0034

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type: Range
persistent identity: range2221559
display id: range2221559
version: 1
top level: BBa_I12024
direction: direction/reverse
start: 64
end: 75
orientation: inline

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation2221563
display id: annotation2221563
version: 1
top level: BBa_I12024
location: https://synbiohub.org/public/igem/BBa_I12024/annotation2221563/range2221563/1
component: BBa_C0051

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type: Range
persistent identity: range2221563
display id: range2221563
version: 1
top level: BBa_I12024
direction: direction/reverse
start: 82
end: 856
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2221570
display id: annotation2221570
version: 1
top level: BBa_I12024
location: https://synbiohub.org/public/igem/BBa_I12024/annotation2221570/range2221570/1
component: BBa_B0015

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type: Range
persistent identity: range2221570
display id: range2221570
version: 1
top level: BBa_I12024
direction: direction/reverse
start: 865
end: 993
orientation: inline

BBa_I12054
type: ComponentDefinition
persistent identity: BBa I12054
display id: BBa_I12054
version: 1
was generated by: iGEM to SBOL conversion
description: B protein (434 cI) subassembly for modified BL oscillator
created: 2004-09-02T11:00:00Z
modified: 2015-08-31T04:07:31Z
owned by: james
owned by: myers
mutable description: This part produces 434 cI (B) in presence of lambda cI (A).
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 145
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: endelman
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I12007
component: BBa_C0056
component: BBa_B0034
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_I12007
sequence annotation: BBa_B0034
sequence annotation: BBa_C0056
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I12054_sequence/1

BBa_I12007
type: Component
persistent identity: component1241967
display id: component1241967
version: 1
top level: BBa_I12054
definition: Prm +
access: public

BBa_C0056
type: Component
persistent identity: component1241980
display id: component1241980
version: 1
top level: BBa_I12054
definition: BBa_C0056
access: public

BBa_B0034
type: Component
persistent identity: component1241972
display id: component1241972
version: 1
top level: BBa_I12054
definition: BBa_B0034
access: public

BBa_B0012
type: Component
persistent identity: component1241995
display id: component1241995
version: 1
top level: BBa_I12054
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1241985
display id: component1241985
version: 1
top level: BBa_I12054
definition: BBa_B0010
access: public

BBa_I12007
type: SequenceAnnotation
persistent identity: annotation1241967
display id: annotation1241967
version: 1
top level: BBa_I12054
location: https://synbiohub.org/public/igem/BBa_I12054/annotation1241967/range1241967/1
component: BBa_I12007

https://synbiohub.org/public/igem/BBa_I12054/annotation1241967/range1241967/1
type: Range
persistent identity: range1241967
display id: range1241967
version: 1
top level: BBa_I12054
direction: direction/forward
start: 1
end: 82
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1241972
display id: annotation1241972
version: 1
top level: BBa_I12054
location: https://synbiohub.org/public/igem/BBa_I12054/annotation1241972/range1241972/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I12054/annotation1241972/range1241972/1
type: Range
persistent identity: range1241972
display id: range1241972
version: 1
top level: BBa_I12054
direction: direction/forward
start: 91
end: 102
orientation: inline

BBa_C0056
type: SequenceAnnotation
persistent identity: annotation1241980
display id: annotation1241980
version: 1
top level: BBa_I12054
location: https://synbiohub.org/public/igem/BBa_I12054/annotation1241980/range1241980/1
component: BBa_C0056

https://synbiohub.org/public/igem/BBa_I12054/annotation1241980/range1241980/1
type: Range
persistent identity: range1241980
display id: range1241980
version: 1
top level: BBa_I12054
direction: direction/forward
start: 109
end: 744
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1241985
display id: annotation1241985
version: 1
top level: BBa_I12054
location: https://synbiohub.org/public/igem/BBa_I12054/annotation1241985/range1241985/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I12054/annotation1241985/range1241985/1
type: Range
persistent identity: range1241985
display id: range1241985
version: 1
top level: BBa_I12054
direction: direction/forward
start: 753
end: 832
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1241995
display id: annotation1241995
version: 1
top level: BBa_I12054
location: https://synbiohub.org/public/igem/BBa_I12054/annotation1241995/range1241995/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I12054/annotation1241995/range1241995/1
type: Range
persistent identity: range1241995
display id: range1241995
version: 1
top level: BBa_I12054
direction: direction/forward
start: 841
end: 881
orientation: inline

BBa_I12056
type: ComponentDefinition
persistent identity: BBa I12056
display id: BBa_I12056
version: 1
was generated by: iGEM to SBOL conversion
description: A protein (lambda cI) subassembly for modified BL oscillator based on I12036
created: 2004-09-02T11:00:00Z
modified: 2015-08-31T04:07:31Z
owned by: james
owned by: myers
mutable description: This part produces POPS output to connect with a reporter.
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 145
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: endelman
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_B0010
component: BBa_C0051
component: BBa_I12036
component: BBa_B0012
component: BBa_B0034
sequence annotation: BBa_I12036
sequence annotation: BBa_B0034
sequence annotation: BBa_C0051
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I12056_sequence/1

BBa_B0010
type: Component
persistent identity: component1241621
display id: component1241621
version: 1
top level: BBa_I12056
definition: BBa_B0010
access: public

BBa_C0051
type: Component
persistent identity: component1241615
display id: component1241615
version: 1
top level: BBa_I12056
definition: cI lam
access: public

BBa_I12036
type: Component
persistent identity: component1241657
display id: component1241657
version: 1
top level: BBa_I12056
definition: BBa_I12036
access: public

BBa_B0012
type: Component
persistent identity: component1241631
display id: component1241631
version: 1
top level: BBa_I12056
definition: BBa_B0012
access: public

BBa_B0034
type: Component
persistent identity: component1241605
display id: component1241605
version: 1
top level: BBa_I12056
definition: BBa_B0034
access: public

BBa_I12036
type: Component
persistent identity: component1241600
display id: component1241600
version: 1
top level: BBa_I12056
definition: BBa_I12036
access: public

BBa_I12036
type: SequenceAnnotation
persistent identity: annotation1241600
display id: annotation1241600
version: 1
top level: BBa_I12056
location: https://synbiohub.org/public/igem/BBa_I12056/annotation1241600/range1241600/1
component: BBa_I12036

https://synbiohub.org/public/igem/BBa_I12056/annotation1241600/range1241600/1
type: Range
persistent identity: range1241600
display id: range1241600
version: 1
top level: BBa_I12056
direction: direction/forward
start: 1
end: 91
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1241605
display id: annotation1241605
version: 1
top level: BBa_I12056
location: https://synbiohub.org/public/igem/BBa_I12056/annotation1241605/range1241605/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I12056/annotation1241605/range1241605/1
type: Range
persistent identity: range1241605
display id: range1241605
version: 1
top level: BBa_I12056
direction: direction/forward
start: 100
end: 111
orientation: inline

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation1241615
display id: annotation1241615
version: 1
top level: BBa_I12056
location: https://synbiohub.org/public/igem/BBa_I12056/annotation1241615/range1241615/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_I12056/annotation1241615/range1241615/1
type: Range
persistent identity: range1241615
display id: range1241615
version: 1
top level: BBa_I12056
direction: direction/forward
start: 118
end: 867
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1241621
display id: annotation1241621
version: 1
top level: BBa_I12056
location: https://synbiohub.org/public/igem/BBa_I12056/annotation1241621/range1241621/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I12056/annotation1241621/range1241621/1
type: Range
persistent identity: range1241621
display id: range1241621
version: 1
top level: BBa_I12056
direction: direction/forward
start: 901
end: 980
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1241631
display id: annotation1241631
version: 1
top level: BBa_I12056
location: https://synbiohub.org/public/igem/BBa_I12056/annotation1241631/range1241631/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I12056/annotation1241631/range1241631/1
type: Range
persistent identity: range1241631
display id: range1241631
version: 1
top level: BBa_I12056
direction: direction/forward
start: 989
end: 1029
orientation: inline

BBa_I12036
type: SequenceAnnotation
persistent identity: annotation1241657
display id: annotation1241657
version: 1
top level: BBa_I12056
location: https://synbiohub.org/public/igem/BBa_I12056/annotation1241657/range1241657/1
component: BBa_I12036

https://synbiohub.org/public/igem/BBa_I12056/annotation1241657/range1241657/1
type: Range
persistent identity: range1241657
display id: range1241657
version: 1
top level: BBa_I12056
direction: direction/forward
start: 1038
end: 1128
orientation: inline

BBa_E0434
type: ComponentDefinition
persistent identity: BBa E0434
display id: BBa_E0434
version: 1
was generated by: iGEM to SBOL conversion
description: B0034.E0034.B0015
created: 2004-07-15T11:00:00Z
modified: 2015-08-31T04:07:26Z
owned by: james
owned by: myers
mutable description: B0034.E0034.B0015 output device with aav tagged yfp
discontinued: false
dominant: true
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: jcbraff
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_E0034
component: BBa_B0012
component: BBa_B0034
component: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_E0034
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_E0434_sequence/1

BBa_E0034
type: Component
persistent identity: component942990
display id: component942990
version: 1
top level: BBa_E0434
definition: EYFP
access: public

BBa_B0012
type: Component
persistent identity: component943005
display id: component943005
version: 1
top level: BBa_E0434
definition: BBa_B0012
access: public

BBa_B0034
type: Component
persistent identity: component942988
display id: component942988
version: 1
top level: BBa_E0434
definition: BBa_B0034
access: public

BBa_B0010
type: Component
persistent identity: component942995
display id: component942995
version: 1
top level: BBa_E0434
definition: BBa_B0010
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation942988
display id: annotation942988
version: 1
top level: BBa_E0434
location: https://synbiohub.org/public/igem/BBa_E0434/annotation942988/range942988/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_E0434/annotation942988/range942988/1
type: Range
persistent identity: range942988
display id: range942988
version: 1
top level: BBa_E0434
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_E0034
type: SequenceAnnotation
persistent identity: annotation942990
display id: annotation942990
version: 1
top level: BBa_E0434
location: https://synbiohub.org/public/igem/BBa_E0434/annotation942990/range942990/1
component: BBa_E0034

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type: Range
persistent identity: range942990
display id: range942990
version: 1
top level: BBa_E0434
direction: direction/forward
start: 19
end: 780
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation942995
display id: annotation942995
version: 1
top level: BBa_E0434
location: https://synbiohub.org/public/igem/BBa_E0434/annotation942995/range942995/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_E0434/annotation942995/range942995/1
type: Range
persistent identity: range942995
display id: range942995
version: 1
top level: BBa_E0434
direction: direction/forward
start: 789
end: 868
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation943005
display id: annotation943005
version: 1
top level: BBa_E0434
location: https://synbiohub.org/public/igem/BBa_E0434/annotation943005/range943005/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_E0434/annotation943005/range943005/1
type: Range
persistent identity: range943005
display id: range943005
version: 1
top level: BBa_E0434
direction: direction/forward
start: 877
end: 917
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

cI lam
type: ComponentDefinition
persistent identity: BBa C0051
display id: BBa_C0051
version: 1
was generated by: iGEM to SBOL conversion
description: cI repressor from E. coli phage lambda (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999).
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the cI repressor based on cI repressor from bacteriophage lambda modified with an LVA tail for rapid degradation of the protein. cI repressor binds to the cI regulator (BBa_R0051).
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001 References (unparsed) here: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001 BBa_C0051 cI repressor is based on the cI repressor from the Elowitz's repressilator. It has been modified to include a rapid degradation LAA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cI lambda
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0051_sequence/1

cI lambda
type: SequenceAnnotation
persistent identity: annotation23334
display id: annotation23334
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation23334/range23334/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0051/annotation23334/range23334/1
type: Range
persistent identity: range23334
display id: range23334
version: 1
top level: cI lam
direction: direction/forward
start: 4
end: 711
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation23335
display id: annotation23335
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation23335/range23335/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0051/annotation23335/range23335/1
type: Range
persistent identity: range23335
display id: range23335
version: 1
top level: cI lam
direction: direction/forward
start: 712
end: 744
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213991
display id: annotation2213991
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation2213991/range2213991/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0051/annotation2213991/range2213991/1
type: Range
persistent identity: range2213991
display id: range2213991
version: 1
top level: cI lam
direction: direction/forward
start: 751
end: 775
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

lacI+pL
type: ComponentDefinition
persistent identity: BBa R0011
display id: BBa_R0011
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (lacI regulated, lambda pL hybrid)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site. LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
star: true
creator: Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0011
sequence annotation: lac O1
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0011_sequence/1

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation7064
display id: annotation7064
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
type: Range
persistent identity: range7064
display id: range7064
version: 1
top level: lacI+pL
direction: direction/forward
start: 1
end: 54
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation1999
display id: annotation1999
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
type: Range
persistent identity: range1999
display id: range1999
version: 1
top level: lacI+pL
direction: direction/forward
start: 3
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2000
display id: annotation2000
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
type: Range
persistent identity: range2000
display id: range2000
version: 1
top level: lacI+pL
direction: direction/forward
start: 20
end: 25
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation2001
display id: annotation2001
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
type: Range
persistent identity: range2001
display id: range2001
version: 1
top level: lacI+pL
direction: direction/forward
start: 26
end: 42
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2002
display id: annotation2002
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
type: Range
persistent identity: range2002
display id: range2002
version: 1
top level: lacI+pL
direction: direction/forward
start: 43
end: 48
orientation: inline

Prm +
type: ComponentDefinition
persistent identity: BBa I12007
display id: BBa_I12007
version: 1
was generated by: iGEM to SBOL conversion
description: Modified lambda Prm promoter (OR-3 obliterated)
created: 2004-07-14T11:00:00Z
modified: 2015-08-31T04:07:31Z
mutable provenance: Shih and Gussin (1983)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Lambda Prm promoter modified to be activated but not repressed by the lambda repressor (cI)
discontinued: false
dominant: true
experience: experience/None
group u list: _3_
m user id: 0
owner id: 103
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In wild type lambda phage, the OR3 site (-27 to -11) reads "tatcccttgcggtgata" on the sense strand of DNA. To prevent lambda repressor (cI) from binding to this site, the 4th through 10th nt of OR3 were replaced by the 7 nt between OR2 and OR1, "aaatagt" (-57 to -51), which in effect mutates 5 nt of the promoter. These nt were chosen to be about halfway between the -10 and -35 boxes of the promoter. Furthermore, since these nt already act as a spacer in wild-type phage, it is hoped they will not create undesired interactions.
star: true
creator: Hans
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OR1
sequence annotation: OR2
sequence annotation: -35
sequence annotation: mutate to obliterate OR3
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_I12007_sequence/1

OR1
type: SequenceAnnotation
persistent identity: annotation937701
display id: annotation937701
version: 1
top level: Prm +
location: https://synbiohub.org/public/igem/BBa_I12007/annotation937701/range937701/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_I12007/annotation937701/range937701/1
type: Range
persistent identity: range937701
display id: range937701
version: 1
top level: Prm +
direction: direction/forward
start: 9
end: 25
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation937702
display id: annotation937702
version: 1
top level: Prm +
location: https://synbiohub.org/public/igem/BBa_I12007/annotation937702/range937702/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_I12007/annotation937702/range937702/1
type: Range
persistent identity: range937702
display id: range937702
version: 1
top level: Prm +
direction: direction/forward
start: 33
end: 49
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation937703
display id: annotation937703
version: 1
top level: Prm +
location: https://synbiohub.org/public/igem/BBa_I12007/annotation937703/range937703/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I12007/annotation937703/range937703/1
type: Range
persistent identity: range937703
display id: range937703
version: 1
top level: Prm +
direction: direction/forward
start: 48
end: 53
orientation: inline

mutate to obliterate OR3
type: SequenceAnnotation
persistent identity: annotation937704
display id: annotation937704
version: 1
top level: Prm +
location: https://synbiohub.org/public/igem/BBa_I12007/annotation937704/range937704/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_I12007/annotation937704/range937704/1
type: Range
persistent identity: range937704
display id: range937704
version: 1
top level: Prm +
direction: direction/forward
start: 59
end: 65
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation937705
display id: annotation937705
version: 1
top level: Prm +
location: https://synbiohub.org/public/igem/BBa_I12007/annotation937705/range937705/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I12007/annotation937705/range937705/1
type: Range
persistent identity: range937705
display id: range937705
version: 1
top level: Prm +
direction: direction/forward
start: 71
end: 76
orientation: inline

BBa_C0056
type: ComponentDefinition
persistent identity: BBa C0056
display id: BBa_C0056
version: 1
was generated by: iGEM to SBOL conversion
description: cI repressor from phage 434 (no LVA)
created: 2004-09-01T11:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Removed LVA tag from BBa_C0052
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Removed LVA tag from BBa_C0052 via PCR. In pSB1AK3 currently.
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cI 434
sequence annotation: 2
sequence: https://synbiohub.org/public/igem/BBa_C0056_sequence/1

cI 434
type: SequenceAnnotation
persistent identity: annotation1063931
display id: annotation1063931
version: 1
top level: BBa_C0056
location: https://synbiohub.org/public/igem/BBa_C0056/annotation1063931/range1063931/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0056/annotation1063931/range1063931/1
type: Range
persistent identity: range1063931
display id: range1063931
version: 1
top level: BBa_C0056
direction: direction/forward
start: 1
end: 636
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation1063930
display id: annotation1063930
version: 1
top level: BBa_C0056
location: https://synbiohub.org/public/igem/BBa_C0056/annotation1063930/range1063930/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_C0056/annotation1063930/range1063930/1
type: Range
persistent identity: range1063930
display id: range1063930
version: 1
top level: BBa_C0056
direction: direction/forward
start: 631
end: 636
orientation: inline

BBa_I12036
type: ComponentDefinition
persistent identity: BBa I12036
display id: BBa_I12036
version: 1
was generated by: iGEM to SBOL conversion
description: Modified lamdba Prm promoter (cooperative repression by 434 cI)
created: 2004-08-17T11:00:00Z
modified: 2015-08-31T04:07:31Z
mutable provenance: Bushman, F. D. The bacteriophage 434 right operator roles of O-R1, O-R2, and O-R3. J. Mol. Biol. (1993) 230, 28-40.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Lamdba Prm promoter modified to be activated by lamda repressor (cI) and repressed by 434 repressor (cI). Two operators are used for both activation and repression to enhance cooperativity.
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 103
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The O-R3 site of lambda (17 nt) was replaced by the O-R1 site of 434 (14 nt) to facilitate repression by 434 cI. The new 434 O-R1 is 5'-justified with the lambda O-R3 it replaced, leaving the -10 sequence intact. The 434 O-R2 site replaces the wildtype labmda promoter starting at -5, thereby preserving the 8 nt between 434 O-R1 and O-R2 in the wildtype. The mRNA transcript will contain an additional 9 nt compared to wildtype lambda phage.
star: true
creator: Hans
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: OR1 lambda
sequence annotation: OR2 lambda
sequence annotation: -35
sequence annotation: OR1 434
sequence annotation: -10
sequence annotation: OR2 434
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_I12036_sequence/1

OR1 lambda
type: SequenceAnnotation
persistent identity: annotation1056735
display id: annotation1056735
version: 1
top level: BBa_I12036
location: https://synbiohub.org/public/igem/BBa_I12036/annotation1056735/range1056735/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_I12036/annotation1056735/range1056735/1
type: Range
persistent identity: range1056735
display id: range1056735
version: 1
top level: BBa_I12036
direction: direction/forward
start: 9
end: 25
orientation: inline

OR2 lambda
type: SequenceAnnotation
persistent identity: annotation1056736
display id: annotation1056736
version: 1
top level: BBa_I12036
location: https://synbiohub.org/public/igem/BBa_I12036/annotation1056736/range1056736/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_I12036/annotation1056736/range1056736/1
type: Range
persistent identity: range1056736
display id: range1056736
version: 1
top level: BBa_I12036
direction: direction/forward
start: 33
end: 49
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1056739
display id: annotation1056739
version: 1
top level: BBa_I12036
location: https://synbiohub.org/public/igem/BBa_I12036/annotation1056739/range1056739/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_I12036/annotation1056739/range1056739/1
type: Range
persistent identity: range1056739
display id: range1056739
version: 1
top level: BBa_I12036
direction: direction/forward
start: 48
end: 53
orientation: inline

OR1 434
type: SequenceAnnotation
persistent identity: annotation1056737
display id: annotation1056737
version: 1
top level: BBa_I12036
location: https://synbiohub.org/public/igem/BBa_I12036/annotation1056737/range1056737/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_I12036/annotation1056737/range1056737/1
type: Range
persistent identity: range1056737
display id: range1056737
version: 1
top level: BBa_I12036
direction: direction/forward
start: 56
end: 69
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1056740
display id: annotation1056740
version: 1
top level: BBa_I12036
location: https://synbiohub.org/public/igem/BBa_I12036/annotation1056740/range1056740/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I12036/annotation1056740/range1056740/1
type: Range
persistent identity: range1056740
display id: range1056740
version: 1
top level: BBa_I12036
direction: direction/forward
start: 71
end: 76
orientation: inline

OR2 434
type: SequenceAnnotation
persistent identity: annotation1056738
display id: annotation1056738
version: 1
top level: BBa_I12036
location: https://synbiohub.org/public/igem/BBa_I12036/annotation1056738/range1056738/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_I12036/annotation1056738/range1056738/1
type: Range
persistent identity: range1056738
display id: range1056738
version: 1
top level: BBa_I12036
direction: direction/forward
start: 78
end: 91
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1062203
display id: annotation1062203
version: 1
top level: BBa_I12036
location: https://synbiohub.org/public/igem/BBa_I12036/annotation1062203/range1062203/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_I12036/annotation1062203/range1062203/1
type: Range
persistent identity: range1062203
display id: range1062203
version: 1
top level: BBa_I12036
direction: direction/forward
start: 83
end: 83
orientation: inline

EYFP
type: ComponentDefinition
persistent identity: BBa E0034
display id: BBa_E0034
version: 1
was generated by: iGEM to SBOL conversion
description: enhanced yellow fluorescent protein derived from A. victoria GFP
created: 2004-06-06T11:00:00Z
modified: 2015-08-31T04:07:25Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000804
role: partType/Reporter
sequence annotation: AAV
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_E0034_sequence/1

AAV
type: SequenceAnnotation
persistent identity: annotation1245672
display id: annotation1245672
version: 1
top level: EYFP
location: https://synbiohub.org/public/igem/BBa_E0034/annotation1245672/range1245672/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_E0034/annotation1245672/range1245672/1
type: Range
persistent identity: range1245672
display id: range1245672
version: 1
top level: EYFP
direction: direction/forward
start: 718
end: 756
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1245671
display id: annotation1245671
version: 1
top level: EYFP
location: https://synbiohub.org/public/igem/BBa_E0034/annotation1245671/range1245671/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_E0034/annotation1245671/range1245671/1
type: Range
persistent identity: range1245671
display id: range1245671
version: 1
top level: EYFP
direction: direction/forward
start: 757
end: 762
orientation: inline

https://synbiohub.org/public/igem/BBa_I12059_sequence/1
type: Sequence
persistent identity: BBa I12059 sequence
display id: BBa_I12059_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttagatatttataaatagtggtgatagatttaacgttactagagaaagaggagaaatactagatgagtatttcttccagggtaaaaagcaaaagaatccagcttggacttaaccaggctgaacttgctcaaaaggtggggactacccagcagtctatagagcagctcgaaaacggtaaaactaagcgaccacgctttttaccagaacttgcgtcagctcttggcgtaagtgttgactggctgctcaatggcacctctgattcgaatgttagatttgttgggcacgttgagcccaaagggaaatatccattgattagcatggttagagctggttcgtggtgtgaagcttgtgaaccctacgatatcaaggacattgatgaatggtatgacagtgacgttaacttattaggcaatggattctggctgaaggttgaaggtgattccatgacctcacctgtaggtcaaagcatccctgaaggtcatatggtgttagtagatactggacgggagccagtgaatggaagccttgttgtagccaaactgactgacgcgaacgaagcaacattcaagaaactggtcatagatggcggtcagaagtacctgaaaggcctgaatccttcatggcctatgactcctatcaacggaaactgcaagattatcggtgttgtcgtggaagcgagggtaaaattcgtataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttagatattacaaactttcttgtatagatttacaatgtatcttgttactagagaaagaggagaa…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I12024_sequence/1
type: Sequence
persistent identity: BBa I12024 sequence
display id: BBa_I12024_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I12054_sequence/1
type: Sequence
persistent identity: BBa I12054 sequence
display id: BBa_I12054_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttagatatttataaatagtggtgatagatttaacgttactagagaaagaggagaaatactagatgagtatttcttccagggtaaaaagcaaaagaatccagcttggacttaaccaggctgaacttgctcaaaaggtggggactacccagcagtctatagagcagctcgaaaacggtaaaactaagcgaccacgctttttaccagaacttgcgtcagctcttggcgtaagtgttgactggctgctcaatggcacctctgattcgaatgttagatttgttgggcacgttgagcccaaagggaaatatccattgattagcatggttagagctggttcgtggtgtgaagcttgtgaaccctacgatatcaaggacattgatgaatggtatgacagtgacgttaacttattaggcaatggattctggctgaaggttgaaggtgattccatgacctcacctgtaggtcaaagcatccctgaaggtcatatggtgttagtagatactggacgggagccagtgaatggaagccttgttgtagccaaactgactgacgcgaacgaagcaacattcaagaaactggtcatagatggcggtcagaagtacctgaaaggcctgaatccttcatggcctatgactcctatcaacggaaactgcaagattatcggtgttgtcgtggaagcgagggtaaaattcgtataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I12056_sequence/1
type: Sequence
persistent identity: BBa I12056 sequence
display id: BBa_I12056_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttagatattacaaactttcttgtatagatttacaatgtatcttgttactagagaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttagatattacaaactttcttgtatagatttacaatgtatcttgt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0434_sequence/1
type: Sequence
persistent identity: BBa E0434 sequence
display id: BBa_E0434_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctgctgctgtttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0051_sequence/1
type: Sequence
persistent identity: BBa C0051 sequence
display id: BBa_C0051_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0011_sequence/1
type: Sequence
persistent identity: BBa R0011 sequence
display id: BBa_R0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I12036_sequence/1
type: Sequence
persistent identity: BBa I12036 sequence
display id: BBa_I12036_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttagatattacaaactttcttgtatagatttacaatgtatcttgt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I12007_sequence/1
type: Sequence
persistent identity: BBa I12007 sequence
display id: BBa_I12007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttagatatttataaatagtggtgatagatttaacgt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0056_sequence/1
type: Sequence
persistent identity: BBa C0056 sequence
display id: BBa_C0056_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagtatttcttccagggtaaaaagcaaaagaatccagcttggacttaaccaggctgaacttgctcaaaaggtggggactacccagcagtctatagagcagctcgaaaacggtaaaactaagcgaccacgctttttaccagaacttgcgtcagctcttggcgtaagtgttgactggctgctcaatggcacctctgattcgaatgttagatttgttgggcacgttgagcccaaagggaaatatccattgattagcatggttagagctggttcgtggtgtgaagcttgtgaaccctacgatatcaaggacattgatgaatggtatgacagtgacgttaacttattaggcaatggattctggctgaaggttgaaggtgattccatgacctcacctgtaggtcaaagcatccctgaaggtcatatggtgttagtagatactggacgggagccagtgaatggaagccttgttgtagccaaactgactgacgcgaacgaagcaacattcaagaaactggtcatagatggcggtcagaagtacctgaaaggcctgaatccttcatggcctatgactcctatcaacggaaactgcaagattatcggtgttgtcgtggaagcgagggtaaaattcgtataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0034_sequence/1
type: Sequence
persistent identity: BBa E0034 sequence
display id: BBa_E0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctgctgctgtttaataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z