BBa_I12100
1
BBa_I12100
2004-07-25T11:00:00Z
2015-08-31T04:07:31Z
false
false
_3_
0
145
7
Not in stock
false
false
endelman
component939391
1
BBa_I12006
annotation939391
1
BBa_I12006
range939391
1
1
82
BBa_I12006
1
Prm +
Modified lamdba Prm promoter (repressed by 434 cI)
2004-07-13T11:00:00Z
2015-08-31T04:07:31Z
Bushman(1993), Shih & Gussin (1983)
Released HQ 2013
Lamdba Prm promoter modified to be activated by lamda repressor (cI) and repressed by 434 repressor (cI)
false
false
_3_
0
147
7
In stock
false
The O-R1 region of 434 contained 14 base pairs as opposed to the 17 base pairs of the O-R3 site of lambda. Also, it was noticed that the O-R3 site of the lambda included part of the -10 site. Hence, to preserve the spacing and the -10 site, the three nucleotides that were in both the -10 site and the lambda O-R3 site were retained. The 14 nucleotides that were in the O-R3 site and not in the -10 site were replaced with the O-R1 site of the 434.
true
mcnamara
annotation786500
1
OR1 lambda
range786500
1
9
25
annotation786518
1
OR2 lambda
range786518
1
33
49
annotation786365
1
OR1 434
range786365
1
56
69
annotation837228
1
-10
range837228
1
71
76
annotation837284
1
-35
range837284
1
48
53
BBa_I12100_sequence
1
gcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttagatattacaaactttcttgtatagatttaacgttactagaggcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttagatatttataaatagtggtgatagatttaacgt
BBa_I12006_sequence
1
gcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttagatattacaaactttcttgtatagatttaacgt
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z