BBa_I12212
1
TetR-4C
TetR - TetR-4C heterodimer promoter (negative)
2004-08-05T11:00:00Z
2015-08-31T04:07:31Z
This promoter is repressed by a heterodimer of wild-type TetR and mutant TetR-4C. It differs from wild-type TetR promoter in that the +4 and -4 regions of its O2 binding site have been changed, and the -35 region of the reverse promoter (pA) has been destroyed.
false
false
_3_
0
120
7
Not in stock
false
The O2 and O1 mutations are all single point mutations, occuring at the first base which appears listed here.
false
rwald
annotation1031337
1
-35
range1031337
1
1
6
annotation1031338
1
TetR O2
range1031338
1
2
20
annotation1031339
1
-35
range1031339
1
22
27
annotation1057574
1
O1 mutation
range1057574
1
37
37
annotation1031342
1
-10
range1031342
1
45
50
annotation1057573
1
O2 mutation
range1057573
1
7
7
annotation1031340
1
-10
range1031340
1
26
30
annotation1031341
1
O1
range1031341
1
32
50
BBa_I12212_sequence
1
ttctctgtcactgatagggagtggtaaaataactctgtcaatgatagagtggattcaaaaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z