LOCUS       BBa_I12226              1775 bp    DNA     linear       12-May-2024
DEFINITION  Generator for wild-type TetR and lambda cI - Gal4 fusion protein
ACCESSION   BBa_I12226
VERSION     BBa_I12226.1  
FEATURES             Location/Qualifiers
     RBS             1..13
                     /label=RBS-3\Weak
     RBS             7..10
                     /label=RBS
     misc_feature    1..13
                     /label=BBa_B0032
     RBS             1..13
                     /label=BBa_B0032
     CDS             20..730
                     /label=cI lambda
     CDS             731..850
                     /label=gal4 dimerization domain
     stem_loop       851..856
                     /label=Terminator
     CDS             20..853
                     /label=cI lambda
     misc_feature    857..881
                     /label=Help:Barcodes
     CDS             20..856
                     /label=BBa_I12200
     misc_feature    890..959
                     /label=Spacer-1
     misc_feature    890..959
                     /label=BBa_B0040
     misc_feature    890..959
                     /label=BBa_B0040
     RBS             968..980
                     /label=RBS-3\Weak
     RBS             974..977
                     /label=RBS
     misc_feature    968..980
                     /label=BBa_B0032
     RBS             968..980
                     /label=BBa_B0032
     CDS             987..1613
                     /label=tetR
     misc_feature    1614..1638
                     /label=Help:Barcodes
     CDS             987..1613
                     /label=BBa_I12202
     stem_loop       1658..1701
                     /label=stem_loop
     misc_feature    1647..1726
                     /label=BBa_B0010
     terminator      1647..1726
                     /label=BBa_B0010
     stem_loop       1742..1761
                     /label=T7 TE
     misc_feature    1768..1768
                     /label=stop
     polyA_site      1762..1775
                     /label=polya
     misc_feature    1735..1775
                     /label=BBa_B0012
     terminator      1735..1775
                     /label=BBa_B0012
ORIGIN
        1 tcacacagga aagtactaga tgagcacaaa aaagaaacca ttaacacaag agcagcttga
       61 ggacgcacgt cgccttaaag caatttatga aaaaaagaaa aatgaacttg gcttatccca
      121 ggaatctgtc gcagacaaga tggggatggg gcagtcaggc gttggtgctt tatttaatgg
      181 catcaatgca ttaaatgctt ataacgccgc attgcttgca aaaattctca aagttagcgt
      241 tgaagaattt agcccttcaa tcgccagaga aatctacgag atgtatgaag cggttagtat
      301 gcagccgtca cttagaagtg agtatgagta ccctgttttt tctcatgttc aggcagggat
      361 gttctcacct gagcttagaa cctttaccaa aggtgatgcg gagagatggg taagcacaac
      421 caaaaaagcc agtgattctg cattctggct tgaggttgaa ggtaattcca tgaccgcacc
      481 aacaggctcc aagccaagct ttcctgacgg aatgttaatt ctcgttgacc ctgagcaggc
      541 tgttgagcca ggtgatttct gcatagccag acttgggggt gatgagttta ccttcaagaa
      601 actgatcagg gatagcggtc aggtgttttt acaaccacta aacccacagt acccaatgat
      661 cccatgcaat gagagttgtt ccgttgtggg gaaagttatc gctagtcagt ggcctgaaga
      721 gacgtttggc gaatcaaggc tagaaagact ggaacagcta tttctactga tttttcctcg
      781 agaagacctt gacatgattt tgaaaatgga ttctttacag gatataaaag cattgttaac
      841 aggattattt taataaccct gatagtgcta gtgtagatcc ctactagaga ggttctgtta
      901 agtaactgaa cccaatgtcg ttagtgacgc ttacctctta agaggtcact gacctaacat
      961 actagagtca cacaggaaag tactagatgt ccagattaga taaaagtaaa gtgattaaca
     1021 gcgcattaga gctgcttaat gaggtcggaa tcgaaggttt aacaacccgt aaactcgccc
     1081 agaagctagg tgtagagcag cctacattgt attggcatgt aaaaaataag cgggctttgc
     1141 tcgacgcctt agccattgag atgttagata ggcaccatac tcacttttgc cctttagaag
     1201 gggaaagctg gcaagatttt ttacgtaata acgctaaaag ttttagatgt gctttactaa
     1261 gtcatcgcga tggagcaaaa gtacatttag gtacacggcc tacagaaaaa cagtatgaaa
     1321 ctctcgaaaa tcaattagcc tttttatgcc aacaaggttt ttcactagag aatgcattat
     1381 atgcactcag cgctgtgggg cattttactt taggttgcgt attggaagat caagagcatc
     1441 aagtcgctaa agaagaaagg gaaacaccta ctactgatag tatgccgcca ttattacgac
     1501 aagctatcga attatttgat caccaaggtg cagagccagc cttcttattc ggccttgaat
     1561 tgatcatatg cggattagaa aaacaactta aatgtgaaag tgggtcttaa taactctgat
     1621 agtgctagtg tagatctcta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa
     1681 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac
     1741 tggctcacct tcgggtgggc ctttctgcgt ttata
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