##gff-version 3
##sequence-region BBa_I12226 1 1775
BBa_I12226	.	terminator	1735	1775	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I12226	.	polyA_site	1762	1775	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I12226	.	engineered_region	1735	1775	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I12226	.	stem_loop	1742	1761	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I12226	.	stop_codon	1768	1768	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I12226	.	sequence_feature	890	959	.	+	0	ID=BBa_B0040;Name=BBa_B0040
BBa_I12226	.	engineered_region	890	959	.	+	0	ID=annotation7030;Name=BBa_B0040;Parent=BBa_B0040
BBa_I12226	.	sequence_feature	890	959	.	+	0	ID=annotation1721;Name=Spacer-1;Parent=BBa_B0040
BBa_I12226	.	ribosome_entry_site	1	13	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_I12226	.	engineered_region	1	13	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_I12226	.	ribosome_entry_site	1	13	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_I12226	.	ribosome_entry_site	7	10	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_I12226	.	CDS	987	1613	.	+	0	ID=BBa_I12202;Name=BBa_I12202
BBa_I12226	.	CDS	987	1613	.	+	0	ID=annotation1031374;Name=tetR;Parent=BBa_I12202
BBa_I12226	.	engineered_tag	1614	1638	.	+	0	ID=annotation2214021;Name=Help:Barcodes;Parent=BBa_I12202
BBa_I12226	.	terminator	1647	1726	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I12226	.	stem_loop	1658	1701	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I12226	.	engineered_region	1647	1726	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I12226	.	ribosome_entry_site	968	980	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_I12226	.	engineered_region	968	980	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_I12226	.	ribosome_entry_site	968	980	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_I12226	.	ribosome_entry_site	974	977	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_I12226	.	CDS	20	856	.	+	0	ID=BBa_I12200;Name=BBa_I12200
BBa_I12226	.	engineered_tag	857	881	.	+	0	ID=annotation2214019;Name=Help:Barcodes;Parent=BBa_I12200
BBa_I12226	.	CDS	20	730	.	+	0	ID=annotation1031160;Name=cI lambda;Parent=BBa_I12200
BBa_I12226	.	stem_loop	851	856	.	+	0	ID=annotation1031174;Name=Terminator;Parent=BBa_I12200
BBa_I12226	.	CDS	20	853	.	+	0	ID=annotation1031472;Name=cI lambda;Parent=BBa_I12200
BBa_I12226	.	CDS	731	850	.	+	0	ID=annotation1031161;Name=gal4 dimerization domain;Parent=BBa_I12200
>BBa_I12226
tcacacaggaaagtactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaa
gcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcag
gcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttag
cgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttaga
agtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtg
atgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgac
cgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggt
gatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttac
aaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtg
gcctgaagagacgtttggcgaatcaaggctagaaagactggaacagctatttctactgatttttcctcgagaagacctt
gacatgattttgaaaatggattctttacaggatataaaagcattgttaacaggattattttaataaccctgatagtgct
agtgtagatccctactagagaggttctgttaagtaactgaacccaatgtcgttagtgacgcttacctcttaagaggtca
ctgacctaacatactagagtcacacaggaaagtactagatgtccagattagataaaagtaaagtgattaacagcgcatt
agagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctaca
ttgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcact
tttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtca
tcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagccttt
ttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtat
tggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgaca
agctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaa
aaacaacttaaatgtgaaagtgggtcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaat
aaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcac
actggctcaccttcgggtgggcctttctgcgtttata