BBa_I12226
type: ComponentDefinition
persistent identity: BBa I12226
display id: BBa_I12226
version: 1
was generated by: iGEM to SBOL conversion
description: Generator for wild-type TetR and lambda cI - Gal4 fusion protein
created: 2004-08-08T11:00:00Z
modified: 2015-08-31T04:07:32Z
owned by: james
owned by: myers
mutable description: This is a generator to create both wild-type TetR and lambda cI - Gal4 fusion protein. It allows one PoPS-in to control two biological ANDs.
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 120
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: rwald
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_B0012
component: BBa_B0032
component: BBa_B0040
component: BBa_B0010
component: BBa_I12202
component: BBa_I12200
sequence annotation: BBa_B0032
sequence annotation: BBa_I12200
sequence annotation: BBa_B0040
sequence annotation: BBa_I12202
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I12226_sequence/1

BBa_B0012
type: Component
persistent identity: component1042059
display id: component1042059
version: 1
top level: BBa_I12226
definition: BBa_B0012
access: public

BBa_B0032
type: Component
persistent identity: component1042035
display id: component1042035
version: 1
top level: BBa_I12226
definition: BBa_B0032
access: public

BBa_B0040
type: Component
persistent identity: component1042027
display id: component1042027
version: 1
top level: BBa_I12226
definition: spacer
access: public

BBa_B0010
type: Component
persistent identity: component1042049
display id: component1042049
version: 1
top level: BBa_I12226
definition: BBa_B0010
access: public

BBa_I12202
type: Component
persistent identity: component1042043
display id: component1042043
version: 1
top level: BBa_I12226
definition: TetR(B)
access: public

BBa_I12200
type: Component
persistent identity: component1042020
display id: component1042020
version: 1
top level: BBa_I12226
definition: fusion
access: public

BBa_B0032
type: Component
persistent identity: component1042003
display id: component1042003
version: 1
top level: BBa_I12226
definition: BBa_B0032
access: public

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1042003
display id: annotation1042003
version: 1
top level: BBa_I12226
location: https://synbiohub.org/public/igem/BBa_I12226/annotation1042003/range1042003/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_I12226/annotation1042003/range1042003/1
type: Range
persistent identity: range1042003
display id: range1042003
version: 1
top level: BBa_I12226
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_I12200
type: SequenceAnnotation
persistent identity: annotation1042020
display id: annotation1042020
version: 1
top level: BBa_I12226
location: https://synbiohub.org/public/igem/BBa_I12226/annotation1042020/range1042020/1
component: BBa_I12200

https://synbiohub.org/public/igem/BBa_I12226/annotation1042020/range1042020/1
type: Range
persistent identity: range1042020
display id: range1042020
version: 1
top level: BBa_I12226
direction: direction/forward
start: 20
end: 856
orientation: inline

BBa_B0040
type: SequenceAnnotation
persistent identity: annotation1042027
display id: annotation1042027
version: 1
top level: BBa_I12226
location: https://synbiohub.org/public/igem/BBa_I12226/annotation1042027/range1042027/1
component: BBa_B0040

https://synbiohub.org/public/igem/BBa_I12226/annotation1042027/range1042027/1
type: Range
persistent identity: range1042027
display id: range1042027
version: 1
top level: BBa_I12226
direction: direction/forward
start: 890
end: 959
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1042035
display id: annotation1042035
version: 1
top level: BBa_I12226
location: https://synbiohub.org/public/igem/BBa_I12226/annotation1042035/range1042035/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_I12226/annotation1042035/range1042035/1
type: Range
persistent identity: range1042035
display id: range1042035
version: 1
top level: BBa_I12226
direction: direction/forward
start: 968
end: 980
orientation: inline

BBa_I12202
type: SequenceAnnotation
persistent identity: annotation1042043
display id: annotation1042043
version: 1
top level: BBa_I12226
location: https://synbiohub.org/public/igem/BBa_I12226/annotation1042043/range1042043/1
component: BBa_I12202

https://synbiohub.org/public/igem/BBa_I12226/annotation1042043/range1042043/1
type: Range
persistent identity: range1042043
display id: range1042043
version: 1
top level: BBa_I12226
direction: direction/forward
start: 987
end: 1613
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1042049
display id: annotation1042049
version: 1
top level: BBa_I12226
location: https://synbiohub.org/public/igem/BBa_I12226/annotation1042049/range1042049/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I12226/annotation1042049/range1042049/1
type: Range
persistent identity: range1042049
display id: range1042049
version: 1
top level: BBa_I12226
direction: direction/forward
start: 1647
end: 1726
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1042059
display id: annotation1042059
version: 1
top level: BBa_I12226
location: https://synbiohub.org/public/igem/BBa_I12226/annotation1042059/range1042059/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I12226/annotation1042059/range1042059/1
type: Range
persistent identity: range1042059
display id: range1042059
version: 1
top level: BBa_I12226
direction: direction/forward
start: 1735
end: 1775
orientation: inline

fusion
type: ComponentDefinition
persistent identity: BBa I12200
display id: BBa_I12200
version: 1
was generated by: iGEM to SBOL conversion
description: Lambda cI - Gal4 fusion protein
created: 2004-08-05T11:00:00Z
modified: 2015-08-31T04:07:31Z
mutable provenance: Lambda phage, Saccharomyces cerevisiae
owned by: james
owned by: myers
mutable description: This is the coding region for the Lambda cI - Gal4 fusion protein. This protein is intended to be used as half of a two-hybrid system, with the other half being Alpha-NTD - Gal11p (BBa_I12201).
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 120
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: rwald
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: cI lambda
sequence annotation: gal4 dimerization domain
sequence annotation: Terminator
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_I12200_sequence/1

cI lambda
type: SequenceAnnotation
persistent identity: annotation1031472
display id: annotation1031472
version: 1
top level: fusion
location: https://synbiohub.org/public/igem/BBa_I12200/annotation1031472/range1031472/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I12200/annotation1031472/range1031472/1
type: Range
persistent identity: range1031472
display id: range1031472
version: 1
top level: fusion
direction: direction/forward
start: 1
end: 834
orientation: inline

cI lambda
type: SequenceAnnotation
persistent identity: annotation1031160
display id: annotation1031160
version: 1
top level: fusion
location: https://synbiohub.org/public/igem/BBa_I12200/annotation1031160/range1031160/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I12200/annotation1031160/range1031160/1
type: Range
persistent identity: range1031160
display id: range1031160
version: 1
top level: fusion
direction: direction/forward
start: 1
end: 711
orientation: inline

gal4 dimerization domain
type: SequenceAnnotation
persistent identity: annotation1031161
display id: annotation1031161
version: 1
top level: fusion
location: https://synbiohub.org/public/igem/BBa_I12200/annotation1031161/range1031161/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I12200/annotation1031161/range1031161/1
type: Range
persistent identity: range1031161
display id: range1031161
version: 1
top level: fusion
direction: direction/forward
start: 712
end: 831
orientation: inline

Terminator
type: SequenceAnnotation
persistent identity: annotation1031174
display id: annotation1031174
version: 1
top level: fusion
location: https://synbiohub.org/public/igem/BBa_I12200/annotation1031174/range1031174/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_I12200/annotation1031174/range1031174/1
type: Range
persistent identity: range1031174
display id: range1031174
version: 1
top level: fusion
direction: direction/forward
start: 832
end: 837
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214019
display id: annotation2214019
version: 1
top level: fusion
location: https://synbiohub.org/public/igem/BBa_I12200/annotation2214019/range2214019/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_I12200/annotation2214019/range2214019/1
type: Range
persistent identity: range2214019
display id: range2214019
version: 1
top level: fusion
direction: direction/forward
start: 838
end: 862
orientation: inline

TetR(B)
type: ComponentDefinition
persistent identity: BBa I12202
display id: BBa_I12202
version: 1
was generated by: iGEM to SBOL conversion
description: Wild-type TetR(B) (-LVA)
created: 2004-08-05T11:00:00Z
modified: 2015-08-31T04:07:31Z
mutable provenance: Serratia marcescens
owned by: james
owned by: myers
mutable description: This is the standard wild-type TetR repressor. It may appear that this is a duplicate of BBa_C0041, but that part has an error near the end which breaks its stop codon. This was presumably to facilitate addition of an LVA tag; nonetheless, for the stand-alone protein, a stop codon is needed.
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 120
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: rwald
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: tetR
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_I12202_sequence/1

tetR
type: SequenceAnnotation
persistent identity: annotation1031374
display id: annotation1031374
version: 1
top level: TetR(B)
location: https://synbiohub.org/public/igem/BBa_I12202/annotation1031374/range1031374/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I12202/annotation1031374/range1031374/1
type: Range
persistent identity: range1031374
display id: range1031374
version: 1
top level: TetR(B)
direction: direction/forward
start: 1
end: 627
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214021
display id: annotation2214021
version: 1
top level: TetR(B)
location: https://synbiohub.org/public/igem/BBa_I12202/annotation2214021/range2214021/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_I12202/annotation2214021/range2214021/1
type: Range
persistent identity: range2214021
display id: range2214021
version: 1
top level: TetR(B)
direction: direction/forward
start: 628
end: 652
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

spacer
type: ComponentDefinition
persistent identity: BBa B0040
display id: BBa_B0040
version: 1
was generated by: iGEM to SBOL conversion
description: Spacer.1 (generic)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Randomly generated and optimized for several parameters (see Design notes).
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Generic spacer for ensuring a 70 bp distance between the end of the suffix of the BioBrick part containing the double terminator and the prefix of the BioBrick part containing the promoter of the new gene. Please, use the AlignX function of Vector NT to check for homology with the components in your plasmid before using this spacer.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The size of the spacer was choosed to meet the minimum length of a sequence that can be queried using the BLAST search engine. However, subsequences of it can be used to design shorter spacers. The sequence was selected from many more sequences randomly generated using the Random DNA Generator engine; the GC% parameter used as input was 50%. The sequences were selected based on the following constraints listed in their order of importance: the absence of any putative promoter regions, a low degree of homology with the Elowitz plasmid (whose components are widely used in our designs), no homology with other E.coli sequences as shown by BLASTN search results and the presence of a number of TAA stop codons. The second constraint was the most stringent leading to the elimination of most sequences. DE made the following changes to the original sequence in order to add stop codons in the -3 frame and more in the +2 frame (note, not all of these stop codons are UAA. Thus, if used in an organism that inserts an amino acid @ UGA or UAG the obvious will occur): T->A @ 85 T->A @ 42 C->T @ 79 A->T @ 64 A->T @ 31 T->A @ 34 C->A @ 37 Also, note that the above changes further reduce (the already very weak) homology to current NCBI-stored sequences. In the process of selecting the best sequence it appeared that a good alternative sequence for a spacer would be: AGGTTCTGATATGTAACTGTGCCCAATGTCGTTAGTGACGCATACCTCTTAAGAGGCCACTGTCCTAACA. The sequence contains no putative promoters and shows moderate homology with the 5' end of the Ampicillin resistance gene. However a strong promoter sequence starts 12 bp downstream of this sequence, and therefore the sequence presented above was preferred. The sequence is compatible (does not show significant homology) with the components in the Elowitz repressilator plasmid.
star: true
creator: Vinay S. Mahajan, Brian Chow, Peter Carr
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: Spacer-1
sequence annotation: BBa_B0040
sequence: https://synbiohub.org/public/igem/BBa_B0040_sequence/1

Spacer-1
type: SequenceAnnotation
persistent identity: annotation1721
display id: annotation1721
version: 1
top level: spacer
location: https://synbiohub.org/public/igem/BBa_B0040/annotation1721/range1721/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_B0040/annotation1721/range1721/1
type: Range
persistent identity: range1721
display id: range1721
version: 1
top level: spacer
direction: direction/forward
start: 1
end: 70
orientation: inline

BBa_B0040
type: SequenceAnnotation
persistent identity: annotation7030
display id: annotation7030
version: 1
top level: spacer
location: https://synbiohub.org/public/igem/BBa_B0040/annotation7030/range7030/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0040/annotation7030/range7030/1
type: Range
persistent identity: range7030
display id: range7030
version: 1
top level: spacer
direction: direction/forward
start: 1
end: 70
orientation: inline

https://synbiohub.org/public/igem/BBa_I12226_sequence/1
type: Sequence
persistent identity: BBa I12226 sequence
display id: BBa_I12226_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaagtactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgaatcaaggctagaaagactggaacagctatttctactgatttttcctcgagaagaccttgacatgattttgaaaatggattctttacaggatataaaagcattgttaacaggattattttaataaccctgatagtgctagtgtagatccctactagagaggttctgttaagtaactgaacccaatgtcgttagtgacgcttacctcttaagaggtcactgacctaacatactagagtcacacaggaaagtactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I12200_sequence/1
type: Sequence
persistent identity: BBa I12200 sequence
display id: BBa_I12200_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgaatcaaggctagaaagactggaacagctatttctactgatttttcctcgagaagaccttgacatgattttgaaaatggattctttacaggatataaaagcattgttaacaggattattttaataaccctgatagtgctagtgtagatccc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I12202_sequence/1
type: Sequence
persistent identity: BBa I12202 sequence
display id: BBa_I12202_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0040_sequence/1
type: Sequence
persistent identity: BBa B0040 sequence
display id: BBa_B0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aggttctgttaagtaactgaacccaatgtcgttagtgacgcttacctcttaagaggtcactgacctaaca
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z