LOCUS       BBa_I12227              1773 bp    DNA     linear       12-May-2024
DEFINITION  Generator for TetR-4C and lambda cI - Gal4 fusion protein
ACCESSION   BBa_I12227
VERSION     BBa_I12227.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..729
                     /label=cI lambda
     CDS             730..849
                     /label=gal4 dimerization domain
     stem_loop       850..855
                     /label=Terminator
     CDS             19..852
                     /label=cI lambda
     misc_feature    856..880
                     /label=Help:Barcodes
     CDS             19..855
                     /label=BBa_I12200
     misc_feature    889..958
                     /label=Spacer-1
     misc_feature    889..958
                     /label=BBa_B0040
     misc_feature    889..958
                     /label=BBa_B0040
     misc_feature    971..974
                     /label=conserved
     RBS             967..978
                     /label=BBa_B0034
     CDS             985..1611
                     /label=tetR-4C
     misc_feature    1612..1636
                     /label=Help:Barcodes
     CDS             985..1611
                     /label=BBa_I12203
     stem_loop       1656..1699
                     /label=stem_loop
     misc_feature    1645..1724
                     /label=BBa_B0010
     terminator      1645..1724
                     /label=BBa_B0010
     stem_loop       1740..1759
                     /label=T7 TE
     misc_feature    1766..1766
                     /label=stop
     polyA_site      1760..1773
                     /label=polya
     misc_feature    1733..1773
                     /label=BBa_B0012
     terminator      1733..1773
                     /label=BBa_B0012
ORIGIN
        1 aaagaggaga aatactagat gagcacaaaa aagaaaccat taacacaaga gcagcttgag
       61 gacgcacgtc gccttaaagc aatttatgaa aaaaagaaaa atgaacttgg cttatcccag
      121 gaatctgtcg cagacaagat ggggatgggg cagtcaggcg ttggtgcttt atttaatggc
      181 atcaatgcat taaatgctta taacgccgca ttgcttgcaa aaattctcaa agttagcgtt
      241 gaagaattta gcccttcaat cgccagagaa atctacgaga tgtatgaagc ggttagtatg
      301 cagccgtcac ttagaagtga gtatgagtac cctgtttttt ctcatgttca ggcagggatg
      361 ttctcacctg agcttagaac ctttaccaaa ggtgatgcgg agagatgggt aagcacaacc
      421 aaaaaagcca gtgattctgc attctggctt gaggttgaag gtaattccat gaccgcacca
      481 acaggctcca agccaagctt tcctgacgga atgttaattc tcgttgaccc tgagcaggct
      541 gttgagccag gtgatttctg catagccaga cttgggggtg atgagtttac cttcaagaaa
      601 ctgatcaggg atagcggtca ggtgttttta caaccactaa acccacagta cccaatgatc
      661 ccatgcaatg agagttgttc cgttgtgggg aaagttatcg ctagtcagtg gcctgaagag
      721 acgtttggcg aatcaaggct agaaagactg gaacagctat ttctactgat ttttcctcga
      781 gaagaccttg acatgatttt gaaaatggat tctttacagg atataaaagc attgttaaca
      841 ggattatttt aataaccctg atagtgctag tgtagatccc tactagagag gttctgttaa
      901 gtaactgaac ccaatgtcgt tagtgacgct tacctcttaa gaggtcactg acctaacata
      961 ctagagaaag aggagaaata ctagatgtcc agattagata aaagtaaagt gattaacagc
     1021 gcattagagc tgcttaatga ggtcggaatc gaaggtttaa caacccgtaa actcgcccag
     1081 aagctaggtg tagcgcagca aacattgatg tggcatgtaa aaaataagcg ggctttgctc
     1141 gacgccttag ccattgagat gttagatagg caccatactc acttttgccc tttagaaggg
     1201 gaaagctggc aagatttttt acgtaataac gctaaaagtt ttagatgtgc tttactaagt
     1261 catcgcgatg gagcaaaagt acatttaggt acacggccta cagaaaaaca gtatgaaact
     1321 ctcgaaaatc aattagcctt tttatgccaa caaggttttt cactagagaa tgcattatat
     1381 gcactcagcg ctgtggggca ttttacttta ggttgcgtat tggaagatca agagcatcaa
     1441 gtcgctaaag aagaaaggga aacacctact actgatagta tgccgccatt attacgacaa
     1501 gctatcgaat tatttgatca ccaaggtgca gagccagcct tcttattcgg ccttgaattg
     1561 atcatatgcg gattagaaaa acaacttaaa tgtgaaagtg ggtcttaata acactgatag
     1621 tgctagtgta gatcactact agagccaggc atcaaataaa acgaaaggct cagtcgaaag
     1681 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
     1741 gctcaccttc gggtgggcct ttctgcgttt ata
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