##gff-version 3
##sequence-region BBa_I12227 1 1773
BBa_I12227	.	terminator	1733	1773	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I12227	.	engineered_region	1733	1773	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I12227	.	stop_codon	1766	1766	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I12227	.	stem_loop	1740	1759	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I12227	.	polyA_site	1760	1773	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I12227	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I12227	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I12227	.	ribosome_entry_site	967	978	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I12227	.	conserved_region	971	974	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I12227	.	CDS	985	1611	.	+	0	ID=BBa_I12203;Name=BBa_I12203
BBa_I12227	.	CDS	985	1611	.	+	0	ID=annotation1031381;Name=tetR-4C;Parent=BBa_I12203
BBa_I12227	.	engineered_tag	1612	1636	.	+	0	ID=annotation2214022;Name=Help:Barcodes;Parent=BBa_I12203
BBa_I12227	.	terminator	1645	1724	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I12227	.	engineered_region	1645	1724	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I12227	.	stem_loop	1656	1699	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I12227	.	sequence_feature	889	958	.	+	0	ID=BBa_B0040;Name=BBa_B0040
BBa_I12227	.	engineered_region	889	958	.	+	0	ID=annotation7030;Name=BBa_B0040;Parent=BBa_B0040
BBa_I12227	.	sequence_feature	889	958	.	+	0	ID=annotation1721;Name=Spacer-1;Parent=BBa_B0040
BBa_I12227	.	CDS	19	855	.	+	0	ID=BBa_I12200;Name=BBa_I12200
BBa_I12227	.	engineered_tag	856	880	.	+	0	ID=annotation2214019;Name=Help:Barcodes;Parent=BBa_I12200
BBa_I12227	.	stem_loop	850	855	.	+	0	ID=annotation1031174;Name=Terminator;Parent=BBa_I12200
BBa_I12227	.	CDS	19	729	.	+	0	ID=annotation1031160;Name=cI lambda;Parent=BBa_I12200
BBa_I12227	.	CDS	19	852	.	+	0	ID=annotation1031472;Name=cI lambda;Parent=BBa_I12200
BBa_I12227	.	CDS	730	849	.	+	0	ID=annotation1031161;Name=gal4 dimerization domain;Parent=BBa_I12200
>BBa_I12227
aaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaag
caatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcagg
cgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagc
gttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaa
gtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtga
tgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgacc
gcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtg
atttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttaca
accactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtgg
cctgaagagacgtttggcgaatcaaggctagaaagactggaacagctatttctactgatttttcctcgagaagaccttg
acatgattttgaaaatggattctttacaggatataaaagcattgttaacaggattattttaataaccctgatagtgcta
gtgtagatccctactagagaggttctgttaagtaactgaacccaatgtcgttagtgacgcttacctcttaagaggtcac
tgacctaacatactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattag
agctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagcgcagcaaacatt
gatgtggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttt
tgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatc
gcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagccttttt
atgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattg
gaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaag
ctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaa
acaacttaaatgtgaaagtgggtcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataa
aacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacac
tggctcaccttcgggtgggcctttctgcgtttata