LOCUS BBa_I12230 5570 bp DNA linear 22-Dec-2024 DEFINITION Two-hybrid system test construct ACCESSION BBa_I12230 VERSION BBa_I12230.1 FEATURES Location/Qualifiers misc_feature 1..3 /label=OR3 misc_feature 11..28 /label=OR2 misc_feature 34..51 /label=OR1 promoter 18..23 /label=-35 promoter 8..13 /label=-35 promoter 30..35 /label=-10 misc_feature 1..54 /label=BBa_R0053 promoter 1..54 /label=BBa_R0053 RBS 63..75 /label=RBS-3\Weak RBS 69..72 /label=RBS misc_feature 63..75 /label=BBa_B0032 RBS 63..75 /label=BBa_B0032 misc_feature 1168..1192 /label=Help:Barcodes CDS 82..1167 /label=BBa_C0013 stem_loop 1212..1255 /label=stem_loop misc_feature 1201..1280 /label=BBa_B0010 terminator 1201..1280 /label=BBa_B0010 stem_loop 1296..1315 /label=T7 TE misc_feature 1322..1322 /label=stop polyA_site 1316..1329 /label=polya misc_feature 1289..1329 /label=BBa_B0012 terminator 1289..1329 /label=BBa_B0012 misc_feature 63..1329 /label=BBa_I12224 misc_feature 1338..1340 /label=OR3 misc_feature 1348..1365 /label=OR2 misc_feature 1371..1388 /label=OR1 promoter 1355..1360 /label=-35 promoter 1345..1350 /label=-35 promoter 1367..1372 /label=-10 misc_feature 1338..1391 /label=BBa_R0053 promoter 1338..1391 /label=BBa_R0053 RBS 1400..1412 /label=RBS-3\Weak RBS 1406..1409 /label=RBS misc_feature 1400..1412 /label=BBa_B0032 RBS 1400..1412 /label=BBa_B0032 CDS 1419..2045 /label=tetR misc_feature 2046..2070 /label=Help:Barcodes CDS 1419..2045 /label=BBa_I12202 stem_loop 2090..2133 /label=stem_loop misc_feature 2079..2158 /label=BBa_B0010 terminator 2079..2158 /label=BBa_B0010 stem_loop 2174..2193 /label=T7 TE misc_feature 2200..2200 /label=stop polyA_site 2194..2207 /label=polya misc_feature 2167..2207 /label=BBa_B0012 terminator 2167..2207 /label=BBa_B0012 misc_feature 1400..2207 /label=BBa_I12222 misc_feature 2218..2234 /label=lac O1 promoter 2235..2240 /label=-35 misc_feature 2241..2257 /label=lac O1 promoter 2258..2263 /label=-10 misc_feature 2216..2269 /label=BBa_R0011 promoter 2216..2269 /label=BBa_R0011 RBS 2279..2291 /label=RBS-3\Weak RBS 2285..2288 /label=RBS misc_feature 2279..2291 /label=BBa_B0032 RBS 2279..2291 /label=BBa_B0032 CDS 2298..3008 /label=cI lambda CDS 3009..3128 /label=gal4 dimerization domain stem_loop 3129..3134 /label=Terminator CDS 2298..3131 /label=cI lambda misc_feature 3135..3159 /label=Help:Barcodes CDS 2298..3134 /label=BBa_I12200 stem_loop 3179..3222 /label=stem_loop misc_feature 3168..3247 /label=BBa_B0010 terminator 3168..3247 /label=BBa_B0010 stem_loop 3263..3282 /label=T7 TE misc_feature 3289..3289 /label=stop polyA_site 3283..3296 /label=polya misc_feature 3256..3296 /label=BBa_B0012 terminator 3256..3296 /label=BBa_B0012 misc_feature 2279..3296 /label=BBa_I12220 misc_feature 3305..3323 /label=TetR 1 misc_feature 3305..3358 /label=BBa_R0040 promoter 3324..3329 /label=-35 misc_feature 3330..3348 /label=TetR 2 promoter 3347..3352 /label=-10 promoter 3305..3358 /label=BBa_R0040 RBS 3367..3379 /label=RBS-3\Weak RBS 3373..3376 /label=RBS misc_feature 3367..3379 /label=BBa_B0032 RBS 3367..3379 /label=BBa_B0032 CDS 3386..4090 /label=Alpha N-terminal domain misc_feature 4091..4129 /label=Flexible linker CDS 4130..4399 /label=Gal11p dimerization domain stem_loop 4400..4405 /label=Terminator CDS 3386..4402 /label=NTD - Gal Alpha misc_feature 4406..4430 /label=Help:Barcodes CDS 3386..4405 /label=BBa_I12201 stem_loop 4450..4493 /label=stem_loop misc_feature 4439..4518 /label=BBa_B0010 terminator 4439..4518 /label=BBa_B0010 stem_loop 4534..4553 /label=T7 TE misc_feature 4560..4560 /label=stop polyA_site 4554..4567 /label=polya misc_feature 4527..4567 /label=BBa_B0012 terminator 4527..4567 /label=BBa_B0012 misc_feature 3367..4567 /label=BBa_I12221 misc_feature 4576..4592 /label=cI OR2 (lambda) promoter 4610..4615 /label=-35 promoter 4634..4640 /label=-10 misc_feature 4593..4609 /label=spacer promoter 4576..4645 /label=BBa_I12210 misc_feature 4658..4661 /label=conserved RBS 4654..4665 /label=BBa_B0034 CDS 4672..5433 /label=CFP (LVA) misc_feature 5390..5427 /label=SsrA misc_feature 5428..5433 /label=2 misc_feature 4740..4740 /label=A misc_feature 4672..5433 /label=BBa_E0022 misc_feature 4672..5433 /label=BBa_E0022 stem_loop 5453..5496 /label=stem_loop misc_feature 5442..5521 /label=BBa_B0010 terminator 5442..5521 /label=BBa_B0010 stem_loop 5537..5556 /label=T7 TE misc_feature 5563..5563 /label=stop polyA_site 5557..5570 /label=polya misc_feature 5530..5570 /label=BBa_B0012 terminator 5530..5570 /label=BBa_B0012 misc_feature 4654..5570 /label=BBa_E0422 ORIGIN 1 aataaacttg actaaagatt cctttagtag ataatttaag tgttctttaa tttctactag 61 agtcacacag gaaagtacta gatgaaacca gtaacgttat acgatgtcgc agagtatgcc 121 ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa 181 acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt acattcccaa ccgcgtggca 241 caacaactgg cgggcaaaca gtcgttgctg attggcgttg ccacctccag tctggccctg 301 cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc 361 gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat 421 cttctcgcgc aacgcgtcag tgggctgatc attaactatc cgctggatga ccaggatgcc 481 attgctgtgg aagctgcctg cactaatgtt ccggcgttat ttcttgatgt ctctgaccag 541 acacccatca acagtattat tttctcccat gaagacggta cgcgactggg cgtggagcat 601 ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg 661 gcgcgtctgc gtctggctgg ctggcataaa tatctcactc gcaatcaaat tcagccgata 721 gcggaacggg aaggcgactg gagtgccatg tccggttttc aacaaaccat gcaaatgctg 781 aatgagggca tcgttcccac tgcgatgctg gttgccaacg atcagatggc gctgggcgca 841 atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg atatctcggt agtgggatac 901 gacgataccg aagacagctc atgttatatc ccgccgttaa ccaccatcaa acaggatttt 961 cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg 1021 aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat 1081 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt 1141 tcccgactgg aaagcgggca gtaataacac tgatagtgct agtgtagatc actactagag 1201 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt 1261 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct 1321 gcgtttatat actagagaat aaacttgact aaagattcct ttagtagata atttaagtgt 1381 tctttaattt ctactagagt cacacaggaa agtactagat gtccagatta gataaaagta 1441 aagtgattaa cagcgcatta gagctgctta atgaggtcgg aatcgaaggt ttaacaaccc 1501 gtaaactcgc ccagaagcta ggtgtagagc agcctacatt gtattggcat gtaaaaaata 1561 agcgggcttt gctcgacgcc ttagccattg agatgttaga taggcaccat actcactttt 1621 gccctttaga aggggaaagc tggcaagatt ttttacgtaa taacgctaaa agttttagat 1681 gtgctttact aagtcatcgc gatggagcaa aagtacattt aggtacacgg cctacagaaa 1741 aacagtatga aactctcgaa aatcaattag cctttttatg ccaacaaggt ttttcactag 1801 agaatgcatt atatgcactc agcgctgtgg ggcattttac tttaggttgc gtattggaag 1861 atcaagagca tcaagtcgct aaagaagaaa gggaaacacc tactactgat agtatgccgc 1921 cattattacg acaagctatc gaattatttg atcaccaagg tgcagagcca gccttcttat 1981 tcggccttga attgatcata tgcggattag aaaaacaact taaatgtgaa agtgggtctt 2041 aataactctg atagtgctag tgtagatctc tactagagcc aggcatcaaa taaaacgaaa 2101 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta 2161 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac tagagaattg 2221 tgagcggata acaattgaca ttgtgagcgg ataacaagat actgagcaca tactagagtc 2281 acacaggaaa gtactagatg agcacaaaaa agaaaccatt aacacaagag cagcttgagg 2341 acgcacgtcg ccttaaagca atttatgaaa aaaagaaaaa tgaacttggc ttatcccagg 2401 aatctgtcgc agacaagatg gggatggggc agtcaggcgt tggtgcttta tttaatggca 2461 tcaatgcatt aaatgcttat aacgccgcat tgcttgcaaa aattctcaaa gttagcgttg 2521 aagaatttag cccttcaatc gccagagaaa tctacgagat gtatgaagcg gttagtatgc 2581 agccgtcact tagaagtgag tatgagtacc ctgttttttc tcatgttcag gcagggatgt 2641 tctcacctga gcttagaacc tttaccaaag gtgatgcgga gagatgggta agcacaacca 2701 aaaaagccag tgattctgca ttctggcttg aggttgaagg taattccatg accgcaccaa 2761 caggctccaa gccaagcttt cctgacggaa tgttaattct cgttgaccct gagcaggctg 2821 ttgagccagg tgatttctgc atagccagac ttgggggtga tgagtttacc ttcaagaaac 2881 tgatcaggga tagcggtcag gtgtttttac aaccactaaa cccacagtac ccaatgatcc 2941 catgcaatga gagttgttcc gttgtgggga aagttatcgc tagtcagtgg cctgaagaga 3001 cgtttggcga atcaaggcta gaaagactgg aacagctatt tctactgatt tttcctcgag 3061 aagaccttga catgattttg aaaatggatt ctttacagga tataaaagca ttgttaacag 3121 gattatttta ataaccctga tagtgctagt gtagatccct actagagcca ggcatcaaat 3181 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa 3241 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact 3301 agagtcccta tcagtgatag agattgacat ccctatcagt gatagagata ctgagcacta 3361 ctagagtcac acaggaaagt actagatgca gggttctgtg acagagtttc taaaaccgcg 3421 cctggttgat atcgagcaag tgagttcgac gcacgccaag gtgacccttg agcctttaga 3481 gcgtggcttt ggccatactc tgggtaacgc actgcgccgt attctgctct catcgatgcc 3541 gggttgcgcg gtgaccgagg ttgagattga tggtgtacta catgagtaca gcaccaaaga 3601 aggcgttcag gaagatatcc tggaaatcct gctcaacctg aaagggctgg cggtgagagt 3661 tcagggcaaa gatgaagtta ttcttacctt gaataaatct ggcattggcc ctgtgacggc 3721 agccgatatc acccacgacg gtgatgtcga aatcgtcaag ccgcagcacg tgatctgcca 3781 cctgaccgat gagaacgcgt ctattagcat gcgtatcaaa gttcagcgcg gtcgtggtta 3841 tgtgccggct tctacccgaa tccattcgga agaagatgag cgcccaatcg gccgtctgct 3901 ggtcgacgca tgctacagcc ctgtggagcg tattgcctac aatgttgaag cagcgcgtgt 3961 agaacagcgt accgacctgg acaagctggt catcgaaatg gaaaccaacg gcacaatcga 4021 tcctgaagag gcgattcgtc gtgcggcaac cattctggct gaacaactgg aagctttcgt 4081 tgacttacgt gatgtacgtc agcctgaagt gaaagaagag aaaccagagc ctcaacagca 4141 gcaaatgcaa cctcccaatt caagtgcgaa caacaaccct ttgcaacagc aatcatcaca 4201 aaataccgta ccaaacgtcc tcaaccaaat taaccaaatc ttttctccag aggagcaacg 4261 cagcttatta caagaagcca tcgaaacctg caagaatttt gaaaaaacac aattgggtag 4321 tacgatgacg gaacctgtca agcaaagttt tattaggaaa tacattaatc aaaaggccct 4381 gagaaaaatc caagctttgt aataacgctg atagtgctag tgtagatcgc tactagagcc 4441 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt 4501 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc 4561 gtttatatac tagagcaaca cgcacggtgt taaggttctg ttaagtaact ttacacttta 4621 tgcttccggc tcgtatgttg tgtggtacta gagaaagagg agaaatacta gatggtgagc 4681 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgtg 4741 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg 4801 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc 4861 accctgacct ggggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac 4921 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac 4981 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc 5041 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag 5101 tacaactaca tcagccacaa cgtctatatc accgccgaca agcagaagaa cggcatcaag 5161 gccaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac 5221 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc 5281 acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag 5341 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagag gcctgctgca 5401 aacgacgaaa actacgcttt agtagcttaa taatactaga gccaggcatc aaataaaacg 5461 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct 5521 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata //