BBa_I12230
type: ComponentDefinition
persistent identity: BBa I12230
display id: BBa_I12230
version: 1
was generated by: iGEM to SBOL conversion
description: Two-hybrid system test construct
created: 2004-08-06T11:00:00Z
modified: 2015-08-31T04:07:32Z
owned by: james
owned by: myers
mutable description: This is a test construct for the two-hybrid system.
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 120
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: rwald
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_E0422
component: BBa_I12210
component: BBa_I12220
component: BBa_R0011
component: BBa_I12221
component: BBa_R0040
component: BBa_I12224
component: BBa_R0053
component: BBa_I12222
sequence annotation: BBa_R0053
sequence annotation: BBa_I12224
sequence annotation: BBa_I12222
sequence annotation: BBa_R0011
sequence annotation: BBa_I12220
sequence annotation: BBa_R0040
sequence annotation: BBa_I12221
sequence annotation: BBa_I12210
sequence annotation: BBa_E0422
sequence: https://synbiohub.org/public/igem/BBa_I12230_sequence/1

BBa_E0422
type: Component
persistent identity: component2239343
display id: component2239343
version: 1
top level: BBa_I12230
definition: BBa_E0422
access: public

BBa_I12210
type: Component
persistent identity: component2239329
display id: component2239329
version: 1
top level: BBa_I12230
definition: lac Or2-62
access: public

BBa_I12220
type: Component
persistent identity: component2239302
display id: component2239302
version: 1
top level: BBa_I12230
definition: BBa_I12220
access: public

BBa_R0011
type: Component
persistent identity: component2239282
display id: component2239282
version: 1
top level: BBa_I12230
definition: lacI+pL
access: public

BBa_I12221
type: Component
persistent identity: component2239324
display id: component2239324
version: 1
top level: BBa_I12230
definition: BBa_I12221
access: public

BBa_R0040
type: Component
persistent identity: component2239303
display id: component2239303
version: 1
top level: BBa_I12230
definition: p(tetR)
access: public

BBa_I12224
type: Component
persistent identity: component2239261
display id: component2239261
version: 1
top level: BBa_I12230
definition: BBa_I12224
access: public

BBa_R0053
type: Component
persistent identity: component2239244
display id: component2239244
version: 1
top level: BBa_I12230
definition: cII p22
access: public

BBa_I12222
type: Component
persistent identity: component2239281
display id: component2239281
version: 1
top level: BBa_I12230
definition: BBa_I12222
access: public

BBa_R0053
type: Component
persistent identity: component2239263
display id: component2239263
version: 1
top level: BBa_I12230
definition: cII p22
access: public

BBa_R0053
type: SequenceAnnotation
persistent identity: annotation2239244
display id: annotation2239244
version: 1
top level: BBa_I12230
location: https://synbiohub.org/public/igem/BBa_I12230/annotation2239244/range2239244/1
component: BBa_R0053

https://synbiohub.org/public/igem/BBa_I12230/annotation2239244/range2239244/1
type: Range
persistent identity: range2239244
display id: range2239244
version: 1
top level: BBa_I12230
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_I12224
type: SequenceAnnotation
persistent identity: annotation2239261
display id: annotation2239261
version: 1
top level: BBa_I12230
location: https://synbiohub.org/public/igem/BBa_I12230/annotation2239261/range2239261/1
component: BBa_I12224

https://synbiohub.org/public/igem/BBa_I12230/annotation2239261/range2239261/1
type: Range
persistent identity: range2239261
display id: range2239261
version: 1
top level: BBa_I12230
direction: direction/reverse
start: 63
end: 1329
orientation: inline

BBa_R0053
type: SequenceAnnotation
persistent identity: annotation2239263
display id: annotation2239263
version: 1
top level: BBa_I12230
location: https://synbiohub.org/public/igem/BBa_I12230/annotation2239263/range2239263/1
component: BBa_R0053

https://synbiohub.org/public/igem/BBa_I12230/annotation2239263/range2239263/1
type: Range
persistent identity: range2239263
display id: range2239263
version: 1
top level: BBa_I12230
direction: direction/forward
start: 1338
end: 1391
orientation: inline

BBa_I12222
type: SequenceAnnotation
persistent identity: annotation2239281
display id: annotation2239281
version: 1
top level: BBa_I12230
location: https://synbiohub.org/public/igem/BBa_I12230/annotation2239281/range2239281/1
component: BBa_I12222

https://synbiohub.org/public/igem/BBa_I12230/annotation2239281/range2239281/1
type: Range
persistent identity: range2239281
display id: range2239281
version: 1
top level: BBa_I12230
direction: direction/reverse
start: 1400
end: 2207
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2239282
display id: annotation2239282
version: 1
top level: BBa_I12230
location: https://synbiohub.org/public/igem/BBa_I12230/annotation2239282/range2239282/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_I12230/annotation2239282/range2239282/1
type: Range
persistent identity: range2239282
display id: range2239282
version: 1
top level: BBa_I12230
direction: direction/forward
start: 2216
end: 2269
orientation: inline

BBa_I12220
type: SequenceAnnotation
persistent identity: annotation2239302
display id: annotation2239302
version: 1
top level: BBa_I12230
location: https://synbiohub.org/public/igem/BBa_I12230/annotation2239302/range2239302/1
component: BBa_I12220

https://synbiohub.org/public/igem/BBa_I12230/annotation2239302/range2239302/1
type: Range
persistent identity: range2239302
display id: range2239302
version: 1
top level: BBa_I12230
direction: direction/reverse
start: 2279
end: 3296
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2239303
display id: annotation2239303
version: 1
top level: BBa_I12230
location: https://synbiohub.org/public/igem/BBa_I12230/annotation2239303/range2239303/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_I12230/annotation2239303/range2239303/1
type: Range
persistent identity: range2239303
display id: range2239303
version: 1
top level: BBa_I12230
direction: direction/forward
start: 3305
end: 3358
orientation: inline

BBa_I12221
type: SequenceAnnotation
persistent identity: annotation2239324
display id: annotation2239324
version: 1
top level: BBa_I12230
location: https://synbiohub.org/public/igem/BBa_I12230/annotation2239324/range2239324/1
component: BBa_I12221

https://synbiohub.org/public/igem/BBa_I12230/annotation2239324/range2239324/1
type: Range
persistent identity: range2239324
display id: range2239324
version: 1
top level: BBa_I12230
direction: direction/reverse
start: 3367
end: 4567
orientation: inline

BBa_I12210
type: SequenceAnnotation
persistent identity: annotation2239329
display id: annotation2239329
version: 1
top level: BBa_I12230
location: https://synbiohub.org/public/igem/BBa_I12230/annotation2239329/range2239329/1
component: BBa_I12210

https://synbiohub.org/public/igem/BBa_I12230/annotation2239329/range2239329/1
type: Range
persistent identity: range2239329
display id: range2239329
version: 1
top level: BBa_I12230
direction: direction/reverse
start: 4576
end: 4645
orientation: inline

BBa_E0422
type: SequenceAnnotation
persistent identity: annotation2239343
display id: annotation2239343
version: 1
top level: BBa_I12230
location: https://synbiohub.org/public/igem/BBa_I12230/annotation2239343/range2239343/1
component: BBa_E0422

https://synbiohub.org/public/igem/BBa_I12230/annotation2239343/range2239343/1
type: Range
persistent identity: range2239343
display id: range2239343
version: 1
top level: BBa_I12230
direction: direction/reverse
start: 4654
end: 5570
orientation: inline

BBa_I12220
type: ComponentDefinition
persistent identity: BBa I12220
display id: BBa_I12220
version: 1
was generated by: iGEM to SBOL conversion
description: Lambda cI - Gal4 fusion protein generator
created: 2004-08-05T11:00:00Z
modified: 2015-08-31T04:07:32Z
owned by: james
owned by: myers
mutable description: Protein generator (with RBS, CDS, and terminators) for lambda cI - Gal4 fusion protein (BBa_I12200).
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 120
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: rwald
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_B0012
component: BBa_B0010
component: BBa_I12200
component: BBa_B0032
sequence annotation: BBa_B0032
sequence annotation: BBa_I12200
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I12220_sequence/1

BBa_B0012
type: Component
persistent identity: component1041808
display id: component1041808
version: 1
top level: BBa_I12220
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1041798
display id: component1041798
version: 1
top level: BBa_I12220
definition: BBa_B0010
access: public

BBa_I12200
type: Component
persistent identity: component1041792
display id: component1041792
version: 1
top level: BBa_I12220
definition: fusion
access: public

BBa_B0032
type: Component
persistent identity: component1041775
display id: component1041775
version: 1
top level: BBa_I12220
definition: BBa_B0032
access: public

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1041775
display id: annotation1041775
version: 1
top level: BBa_I12220
location: https://synbiohub.org/public/igem/BBa_I12220/annotation1041775/range1041775/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_I12220/annotation1041775/range1041775/1
type: Range
persistent identity: range1041775
display id: range1041775
version: 1
top level: BBa_I12220
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_I12200
type: SequenceAnnotation
persistent identity: annotation1041792
display id: annotation1041792
version: 1
top level: BBa_I12220
location: https://synbiohub.org/public/igem/BBa_I12220/annotation1041792/range1041792/1
component: BBa_I12200

https://synbiohub.org/public/igem/BBa_I12220/annotation1041792/range1041792/1
type: Range
persistent identity: range1041792
display id: range1041792
version: 1
top level: BBa_I12220
direction: direction/forward
start: 20
end: 856
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1041798
display id: annotation1041798
version: 1
top level: BBa_I12220
location: https://synbiohub.org/public/igem/BBa_I12220/annotation1041798/range1041798/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I12220/annotation1041798/range1041798/1
type: Range
persistent identity: range1041798
display id: range1041798
version: 1
top level: BBa_I12220
direction: direction/forward
start: 890
end: 969
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1041808
display id: annotation1041808
version: 1
top level: BBa_I12220
location: https://synbiohub.org/public/igem/BBa_I12220/annotation1041808/range1041808/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I12220/annotation1041808/range1041808/1
type: Range
persistent identity: range1041808
display id: range1041808
version: 1
top level: BBa_I12220
direction: direction/forward
start: 978
end: 1018
orientation: inline

BBa_I12221
type: ComponentDefinition
persistent identity: BBa I12221
display id: BBa_I12221
version: 1
was generated by: iGEM to SBOL conversion
description: Alpha-NTD - Gal11p fusion protein generator
created: 2004-08-06T11:00:00Z
modified: 2015-08-31T04:07:32Z
owned by: james
owned by: myers
mutable description: Protein generator (with RBS, CDS, and terminators) for alpha-NTD - Gal11p fusion protein (BBa_I12201).
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 120
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: rwald
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_I12201
component: BBa_B0032
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0032
sequence annotation: BBa_I12201
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I12221_sequence/1

BBa_I12201
type: Component
persistent identity: component1041839
display id: component1041839
version: 1
top level: BBa_I12221
definition: Fusion
access: public

BBa_B0032
type: Component
persistent identity: component1041819
display id: component1041819
version: 1
top level: BBa_I12221
definition: BBa_B0032
access: public

BBa_B0012
type: Component
persistent identity: component1041855
display id: component1041855
version: 1
top level: BBa_I12221
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1041845
display id: component1041845
version: 1
top level: BBa_I12221
definition: BBa_B0010
access: public

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1041819
display id: annotation1041819
version: 1
top level: BBa_I12221
location: https://synbiohub.org/public/igem/BBa_I12221/annotation1041819/range1041819/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_I12221/annotation1041819/range1041819/1
type: Range
persistent identity: range1041819
display id: range1041819
version: 1
top level: BBa_I12221
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_I12201
type: SequenceAnnotation
persistent identity: annotation1041839
display id: annotation1041839
version: 1
top level: BBa_I12221
location: https://synbiohub.org/public/igem/BBa_I12221/annotation1041839/range1041839/1
component: BBa_I12201

https://synbiohub.org/public/igem/BBa_I12221/annotation1041839/range1041839/1
type: Range
persistent identity: range1041839
display id: range1041839
version: 1
top level: BBa_I12221
direction: direction/forward
start: 20
end: 1039
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1041845
display id: annotation1041845
version: 1
top level: BBa_I12221
location: https://synbiohub.org/public/igem/BBa_I12221/annotation1041845/range1041845/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I12221/annotation1041845/range1041845/1
type: Range
persistent identity: range1041845
display id: range1041845
version: 1
top level: BBa_I12221
direction: direction/forward
start: 1073
end: 1152
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1041855
display id: annotation1041855
version: 1
top level: BBa_I12221
location: https://synbiohub.org/public/igem/BBa_I12221/annotation1041855/range1041855/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I12221/annotation1041855/range1041855/1
type: Range
persistent identity: range1041855
display id: range1041855
version: 1
top level: BBa_I12221
direction: direction/forward
start: 1161
end: 1201
orientation: inline

BBa_I12222
type: ComponentDefinition
persistent identity: BBa I12222
display id: BBa_I12222
version: 1
was generated by: iGEM to SBOL conversion
description: Wild-type TetR(B) protein generator
created: 2004-08-06T11:00:00Z
modified: 2015-08-31T04:07:32Z
owned by: james
owned by: myers
mutable description: Protein generator (with RBS, CDS, and terminators) for Wild-type TetR(B) (BBa_I12202).
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 120
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: rwald
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0012
component: BBa_B0010
component: BBa_I12202
component: BBa_B0032
sequence annotation: BBa_B0032
sequence annotation: BBa_I12202
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I12222_sequence/1

BBa_B0012
type: Component
persistent identity: component1041890
display id: component1041890
version: 1
top level: BBa_I12222
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1041880
display id: component1041880
version: 1
top level: BBa_I12222
definition: BBa_B0010
access: public

BBa_I12202
type: Component
persistent identity: component1041874
display id: component1041874
version: 1
top level: BBa_I12222
definition: TetR(B)
access: public

BBa_B0032
type: Component
persistent identity: component1041866
display id: component1041866
version: 1
top level: BBa_I12222
definition: BBa_B0032
access: public

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1041866
display id: annotation1041866
version: 1
top level: BBa_I12222
location: https://synbiohub.org/public/igem/BBa_I12222/annotation1041866/range1041866/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_I12222/annotation1041866/range1041866/1
type: Range
persistent identity: range1041866
display id: range1041866
version: 1
top level: BBa_I12222
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_I12202
type: SequenceAnnotation
persistent identity: annotation1041874
display id: annotation1041874
version: 1
top level: BBa_I12222
location: https://synbiohub.org/public/igem/BBa_I12222/annotation1041874/range1041874/1
component: BBa_I12202

https://synbiohub.org/public/igem/BBa_I12222/annotation1041874/range1041874/1
type: Range
persistent identity: range1041874
display id: range1041874
version: 1
top level: BBa_I12222
direction: direction/forward
start: 20
end: 646
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1041880
display id: annotation1041880
version: 1
top level: BBa_I12222
location: https://synbiohub.org/public/igem/BBa_I12222/annotation1041880/range1041880/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I12222/annotation1041880/range1041880/1
type: Range
persistent identity: range1041880
display id: range1041880
version: 1
top level: BBa_I12222
direction: direction/forward
start: 680
end: 759
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1041890
display id: annotation1041890
version: 1
top level: BBa_I12222
location: https://synbiohub.org/public/igem/BBa_I12222/annotation1041890/range1041890/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I12222/annotation1041890/range1041890/1
type: Range
persistent identity: range1041890
display id: range1041890
version: 1
top level: BBa_I12222
direction: direction/forward
start: 768
end: 808
orientation: inline

BBa_I12224
type: ComponentDefinition
persistent identity: BBa I12224
display id: BBa_I12224
version: 1
was generated by: iGEM to SBOL conversion
description: LacI protein generator (LVA-)
created: 2004-08-06T11:00:00Z
modified: 2015-08-31T04:07:32Z
owned by: james
owned by: myers
mutable description: Protein generator for LacI (LVA-).
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 120
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: rwald
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0012
component: BBa_B0010
component: BBa_C0013
component: BBa_B0032
sequence annotation: BBa_B0032
sequence annotation: BBa_C0013
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I12224_sequence/1

BBa_B0012
type: Component
persistent identity: component1041957
display id: component1041957
version: 1
top level: BBa_I12224
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1041947
display id: component1041947
version: 1
top level: BBa_I12224
definition: BBa_B0010
access: public

BBa_C0013
type: Component
persistent identity: component1041941
display id: component1041941
version: 1
top level: BBa_I12224
definition: lacI
access: public

BBa_B0032
type: Component
persistent identity: component1041936
display id: component1041936
version: 1
top level: BBa_I12224
definition: BBa_B0032
access: public

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1041936
display id: annotation1041936
version: 1
top level: BBa_I12224
location: https://synbiohub.org/public/igem/BBa_I12224/annotation1041936/range1041936/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_I12224/annotation1041936/range1041936/1
type: Range
persistent identity: range1041936
display id: range1041936
version: 1
top level: BBa_I12224
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_C0013
type: SequenceAnnotation
persistent identity: annotation1041941
display id: annotation1041941
version: 1
top level: BBa_I12224
location: https://synbiohub.org/public/igem/BBa_I12224/annotation1041941/range1041941/1
component: BBa_C0013

https://synbiohub.org/public/igem/BBa_I12224/annotation1041941/range1041941/1
type: Range
persistent identity: range1041941
display id: range1041941
version: 1
top level: BBa_I12224
direction: direction/forward
start: 20
end: 1105
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1041947
display id: annotation1041947
version: 1
top level: BBa_I12224
location: https://synbiohub.org/public/igem/BBa_I12224/annotation1041947/range1041947/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I12224/annotation1041947/range1041947/1
type: Range
persistent identity: range1041947
display id: range1041947
version: 1
top level: BBa_I12224
direction: direction/forward
start: 1139
end: 1218
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1041957
display id: annotation1041957
version: 1
top level: BBa_I12224
location: https://synbiohub.org/public/igem/BBa_I12224/annotation1041957/range1041957/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I12224/annotation1041957/range1041957/1
type: Range
persistent identity: range1041957
display id: range1041957
version: 1
top level: BBa_I12224
direction: direction/forward
start: 1227
end: 1267
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

lacI+pL
type: ComponentDefinition
persistent identity: BBa R0011
display id: BBa_R0011
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (lacI regulated, lambda pL hybrid)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site. LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
star: true
creator: Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0011
sequence annotation: lac O1
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0011_sequence/1

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation7064
display id: annotation7064
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
type: Range
persistent identity: range7064
display id: range7064
version: 1
top level: lacI+pL
direction: direction/forward
start: 1
end: 54
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation1999
display id: annotation1999
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
type: Range
persistent identity: range1999
display id: range1999
version: 1
top level: lacI+pL
direction: direction/forward
start: 3
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2000
display id: annotation2000
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
type: Range
persistent identity: range2000
display id: range2000
version: 1
top level: lacI+pL
direction: direction/forward
start: 20
end: 25
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation2001
display id: annotation2001
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
type: Range
persistent identity: range2001
display id: range2001
version: 1
top level: lacI+pL
direction: direction/forward
start: 26
end: 42
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2002
display id: annotation2002
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
type: Range
persistent identity: range2002
display id: range2002
version: 1
top level: lacI+pL
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_E0422
type: ComponentDefinition
persistent identity: BBa E0422
display id: BBa_E0422
version: 1
was generated by: iGEM to SBOL conversion
description: ECFP (RBS+ LVA+ TERM) (B0034.E0022.B0015)
created: 2003-12-04T12:00:00Z
modified: 2015-08-31T04:07:26Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: -- No description --
discontinued: false
dominant: true
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Caitlin Conboy and Jennifer Braff
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0034
component: BBa_B0010
component: BBa_E0022
component: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_E0022
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_E0422_sequence/1

BBa_B0034
type: Component
persistent identity: component942803
display id: component942803
version: 1
top level: BBa_E0422
definition: BBa_B0034
access: public

BBa_B0010
type: Component
persistent identity: component942823
display id: component942823
version: 1
top level: BBa_E0422
definition: BBa_B0010
access: public

BBa_E0022
type: Component
persistent identity: component942815
display id: component942815
version: 1
top level: BBa_E0422
definition: ECFP
access: public

BBa_B0012
type: Component
persistent identity: component942833
display id: component942833
version: 1
top level: BBa_E0422
definition: BBa_B0012
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation942803
display id: annotation942803
version: 1
top level: BBa_E0422
location: https://synbiohub.org/public/igem/BBa_E0422/annotation942803/range942803/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_E0422/annotation942803/range942803/1
type: Range
persistent identity: range942803
display id: range942803
version: 1
top level: BBa_E0422
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_E0022
type: SequenceAnnotation
persistent identity: annotation942815
display id: annotation942815
version: 1
top level: BBa_E0422
location: https://synbiohub.org/public/igem/BBa_E0422/annotation942815/range942815/1
component: BBa_E0022

https://synbiohub.org/public/igem/BBa_E0422/annotation942815/range942815/1
type: Range
persistent identity: range942815
display id: range942815
version: 1
top level: BBa_E0422
direction: direction/forward
start: 19
end: 780
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation942823
display id: annotation942823
version: 1
top level: BBa_E0422
location: https://synbiohub.org/public/igem/BBa_E0422/annotation942823/range942823/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_E0422/annotation942823/range942823/1
type: Range
persistent identity: range942823
display id: range942823
version: 1
top level: BBa_E0422
direction: direction/forward
start: 789
end: 868
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation942833
display id: annotation942833
version: 1
top level: BBa_E0422
location: https://synbiohub.org/public/igem/BBa_E0422/annotation942833/range942833/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_E0422/annotation942833/range942833/1
type: Range
persistent identity: range942833
display id: range942833
version: 1
top level: BBa_E0422
direction: direction/forward
start: 877
end: 917
orientation: inline

cII p22
type: ComponentDefinition
persistent identity: BBa R0053
display id: BBa_R0053
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (p22 cII regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Bacteriophage p22.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The p22 cII regulatory region sequence is a 97 base-pair sequence with the standard BioBrick prefix and suffix sections on its ends. p22 cII repressor protein, BBa_C0053, binds to it. This segment contains O-R1, O-R2, a fragment of O-R3, the -35 of P-RM, and P-R (-10 and -35 from Tom Knight)
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Maia Mahoney
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0053
sequence annotation: OR3
sequence annotation: -35
sequence annotation: OR2
sequence annotation: -10
sequence annotation: OR1
sequence: https://synbiohub.org/public/igem/BBa_R0053_sequence/1

BBa_R0053
type: SequenceAnnotation
persistent identity: annotation7069
display id: annotation7069
version: 1
top level: cII p22
location: https://synbiohub.org/public/igem/BBa_R0053/annotation7069/range7069/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0053/annotation7069/range7069/1
type: Range
persistent identity: range7069
display id: range7069
version: 1
top level: cII p22
direction: direction/forward
start: 1
end: 54
orientation: inline

OR3
type: SequenceAnnotation
persistent identity: annotation2035
display id: annotation2035
version: 1
top level: cII p22
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2035/range2035/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0053/annotation2035/range2035/1
type: Range
persistent identity: range2035
display id: range2035
version: 1
top level: cII p22
direction: direction/forward
start: 1
end: 3
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2041
display id: annotation2041
version: 1
top level: cII p22
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2041/range2041/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0053/annotation2041/range2041/1
type: Range
persistent identity: range2041
display id: range2041
version: 1
top level: cII p22
direction: direction/forward
start: 8
end: 13
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2036
display id: annotation2036
version: 1
top level: cII p22
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2036/range2036/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0053/annotation2036/range2036/1
type: Range
persistent identity: range2036
display id: range2036
version: 1
top level: cII p22
direction: direction/forward
start: 11
end: 28
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2038
display id: annotation2038
version: 1
top level: cII p22
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2038/range2038/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0053/annotation2038/range2038/1
type: Range
persistent identity: range2038
display id: range2038
version: 1
top level: cII p22
direction: direction/forward
start: 18
end: 23
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2042
display id: annotation2042
version: 1
top level: cII p22
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2042/range2042/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0053/annotation2042/range2042/1
type: Range
persistent identity: range2042
display id: range2042
version: 1
top level: cII p22
direction: direction/forward
start: 30
end: 35
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation2037
display id: annotation2037
version: 1
top level: cII p22
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2037/range2037/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0053/annotation2037/range2037/1
type: Range
persistent identity: range2037
display id: range2037
version: 1
top level: cII p22
direction: direction/forward
start: 34
end: 51
orientation: inline

lac Or2-62
type: ComponentDefinition
persistent identity: BBa I12210
display id: BBa_I12210
version: 1
was generated by: iGEM to SBOL conversion
description: plac Or2-62 (positive)
created: 2004-08-06T11:00:00Z
modified: 2015-08-31T04:07:31Z
owned by: james
owned by: myers
mutable description: This is a modification of the standard plac operon with everything before the -35 site removed, the Or2 site from the lambda promoter inserted at the beginning, and a 17-bp spacer added between the two.
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 120
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: rwald
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: cI OR2 (lambda)
sequence annotation: spacer
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_I12210_sequence/1

cI OR2 (lambda)
type: SequenceAnnotation
persistent identity: annotation1031724
display id: annotation1031724
version: 1
top level: lac Or2-62
location: https://synbiohub.org/public/igem/BBa_I12210/annotation1031724/range1031724/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_I12210/annotation1031724/range1031724/1
type: Range
persistent identity: range1031724
display id: range1031724
version: 1
top level: lac Or2-62
direction: direction/forward
start: 1
end: 17
orientation: inline

spacer
type: SequenceAnnotation
persistent identity: annotation1031727
display id: annotation1031727
version: 1
top level: lac Or2-62
location: https://synbiohub.org/public/igem/BBa_I12210/annotation1031727/range1031727/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I12210/annotation1031727/range1031727/1
type: Range
persistent identity: range1031727
display id: range1031727
version: 1
top level: lac Or2-62
direction: direction/forward
start: 18
end: 34
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1031725
display id: annotation1031725
version: 1
top level: lac Or2-62
location: https://synbiohub.org/public/igem/BBa_I12210/annotation1031725/range1031725/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_I12210/annotation1031725/range1031725/1
type: Range
persistent identity: range1031725
display id: range1031725
version: 1
top level: lac Or2-62
direction: direction/forward
start: 35
end: 40
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1031726
display id: annotation1031726
version: 1
top level: lac Or2-62
location: https://synbiohub.org/public/igem/BBa_I12210/annotation1031726/range1031726/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I12210/annotation1031726/range1031726/1
type: Range
persistent identity: range1031726
display id: range1031726
version: 1
top level: lac Or2-62
direction: direction/forward
start: 59
end: 65
orientation: inline

fusion
type: ComponentDefinition
persistent identity: BBa I12200
display id: BBa_I12200
version: 1
was generated by: iGEM to SBOL conversion
description: Lambda cI - Gal4 fusion protein
created: 2004-08-05T11:00:00Z
modified: 2015-08-31T04:07:31Z
mutable provenance: Lambda phage, Saccharomyces cerevisiae
owned by: james
owned by: myers
mutable description: This is the coding region for the Lambda cI - Gal4 fusion protein. This protein is intended to be used as half of a two-hybrid system, with the other half being Alpha-NTD - Gal11p (BBa_I12201).
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 120
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: rwald
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cI lambda
sequence annotation: gal4 dimerization domain
sequence annotation: Terminator
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_I12200_sequence/1

cI lambda
type: SequenceAnnotation
persistent identity: annotation1031472
display id: annotation1031472
version: 1
top level: fusion
location: https://synbiohub.org/public/igem/BBa_I12200/annotation1031472/range1031472/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I12200/annotation1031472/range1031472/1
type: Range
persistent identity: range1031472
display id: range1031472
version: 1
top level: fusion
direction: direction/forward
start: 1
end: 834
orientation: inline

cI lambda
type: SequenceAnnotation
persistent identity: annotation1031160
display id: annotation1031160
version: 1
top level: fusion
location: https://synbiohub.org/public/igem/BBa_I12200/annotation1031160/range1031160/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I12200/annotation1031160/range1031160/1
type: Range
persistent identity: range1031160
display id: range1031160
version: 1
top level: fusion
direction: direction/forward
start: 1
end: 711
orientation: inline

gal4 dimerization domain
type: SequenceAnnotation
persistent identity: annotation1031161
display id: annotation1031161
version: 1
top level: fusion
location: https://synbiohub.org/public/igem/BBa_I12200/annotation1031161/range1031161/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I12200/annotation1031161/range1031161/1
type: Range
persistent identity: range1031161
display id: range1031161
version: 1
top level: fusion
direction: direction/forward
start: 712
end: 831
orientation: inline

Terminator
type: SequenceAnnotation
persistent identity: annotation1031174
display id: annotation1031174
version: 1
top level: fusion
location: https://synbiohub.org/public/igem/BBa_I12200/annotation1031174/range1031174/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_I12200/annotation1031174/range1031174/1
type: Range
persistent identity: range1031174
display id: range1031174
version: 1
top level: fusion
direction: direction/forward
start: 832
end: 837
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214019
display id: annotation2214019
version: 1
top level: fusion
location: https://synbiohub.org/public/igem/BBa_I12200/annotation2214019/range2214019/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_I12200/annotation2214019/range2214019/1
type: Range
persistent identity: range2214019
display id: range2214019
version: 1
top level: fusion
direction: direction/forward
start: 838
end: 862
orientation: inline

Fusion
type: ComponentDefinition
persistent identity: BBa I12201
display id: BBa_I12201
version: 1
was generated by: iGEM to SBOL conversion
description: Alpha-NTD - Gal11p fusion protein
created: 2004-08-05T11:00:00Z
modified: 2015-08-31T04:07:31Z
mutable provenance: Escherichia coli, Saccharomyces cerevisiae
owned by: james
owned by: myers
mutable description: This is the alpha N-terminal domain - Gal11p fusion protein. It is meant to be used as part of a two-hybrid system along with lambda cI - Gal4 (BBa_I12200).
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 120
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: rwald
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: Alpha N-terminal domain
sequence annotation: NTD - Gal Alpha
sequence annotation: Flexible linker
sequence annotation: Gal11p dimerization domain
sequence annotation: Terminator
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_I12201_sequence/1

Alpha N-terminal domain
type: SequenceAnnotation
persistent identity: annotation1031181
display id: annotation1031181
version: 1
top level: Fusion
location: https://synbiohub.org/public/igem/BBa_I12201/annotation1031181/range1031181/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I12201/annotation1031181/range1031181/1
type: Range
persistent identity: range1031181
display id: range1031181
version: 1
top level: Fusion
direction: direction/forward
start: 1
end: 705
orientation: inline

NTD - Gal Alpha
type: SequenceAnnotation
persistent identity: annotation1031564
display id: annotation1031564
version: 1
top level: Fusion
location: https://synbiohub.org/public/igem/BBa_I12201/annotation1031564/range1031564/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I12201/annotation1031564/range1031564/1
type: Range
persistent identity: range1031564
display id: range1031564
version: 1
top level: Fusion
direction: direction/forward
start: 1
end: 1017
orientation: inline

Flexible linker
type: SequenceAnnotation
persistent identity: annotation1031182
display id: annotation1031182
version: 1
top level: Fusion
location: https://synbiohub.org/public/igem/BBa_I12201/annotation1031182/range1031182/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_I12201/annotation1031182/range1031182/1
type: Range
persistent identity: range1031182
display id: range1031182
version: 1
top level: Fusion
direction: direction/forward
start: 706
end: 744
orientation: inline

Gal11p dimerization domain
type: SequenceAnnotation
persistent identity: annotation1031183
display id: annotation1031183
version: 1
top level: Fusion
location: https://synbiohub.org/public/igem/BBa_I12201/annotation1031183/range1031183/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I12201/annotation1031183/range1031183/1
type: Range
persistent identity: range1031183
display id: range1031183
version: 1
top level: Fusion
direction: direction/forward
start: 745
end: 1014
orientation: inline

Terminator
type: SequenceAnnotation
persistent identity: annotation1031184
display id: annotation1031184
version: 1
top level: Fusion
location: https://synbiohub.org/public/igem/BBa_I12201/annotation1031184/range1031184/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_I12201/annotation1031184/range1031184/1
type: Range
persistent identity: range1031184
display id: range1031184
version: 1
top level: Fusion
direction: direction/forward
start: 1015
end: 1020
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214020
display id: annotation2214020
version: 1
top level: Fusion
location: https://synbiohub.org/public/igem/BBa_I12201/annotation2214020/range2214020/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_I12201/annotation2214020/range2214020/1
type: Range
persistent identity: range2214020
display id: range2214020
version: 1
top level: Fusion
direction: direction/forward
start: 1021
end: 1045
orientation: inline

TetR(B)
type: ComponentDefinition
persistent identity: BBa I12202
display id: BBa_I12202
version: 1
was generated by: iGEM to SBOL conversion
description: Wild-type TetR(B) (-LVA)
created: 2004-08-05T11:00:00Z
modified: 2015-08-31T04:07:31Z
mutable provenance: Serratia marcescens
owned by: james
owned by: myers
mutable description: This is the standard wild-type TetR repressor. It may appear that this is a duplicate of BBa_C0041, but that part has an error near the end which breaks its stop codon. This was presumably to facilitate addition of an LVA tag; nonetheless, for the stand-alone protein, a stop codon is needed.
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 120
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: rwald
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: tetR
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_I12202_sequence/1

tetR
type: SequenceAnnotation
persistent identity: annotation1031374
display id: annotation1031374
version: 1
top level: TetR(B)
location: https://synbiohub.org/public/igem/BBa_I12202/annotation1031374/range1031374/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I12202/annotation1031374/range1031374/1
type: Range
persistent identity: range1031374
display id: range1031374
version: 1
top level: TetR(B)
direction: direction/forward
start: 1
end: 627
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214021
display id: annotation2214021
version: 1
top level: TetR(B)
location: https://synbiohub.org/public/igem/BBa_I12202/annotation2214021/range2214021/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_I12202/annotation2214021/range2214021/1
type: Range
persistent identity: range2214021
display id: range2214021
version: 1
top level: TetR(B)
direction: direction/forward
start: 628
end: 652
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

lacI
type: ComponentDefinition
persistent identity: BBa C0013
display id: BBa_C0013
version: 1
was generated by: iGEM to SBOL conversion
description: Repressor, lacI
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: www.ncbi.nlm.nih.gov
owned by: james
owned by: myers
mutable description: LacI repressor protein, without SsrA degradation tag. No Barcode.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Yong-Su Jin (Fighting Darwins)
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0013_sequence/1

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214005
display id: annotation2214005
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0013/annotation2214005/range2214005/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0013/annotation2214005/range2214005/1
type: Range
persistent identity: range2214005
display id: range2214005
version: 1
top level: lacI
direction: direction/forward
start: 1087
end: 1111
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

ECFP
type: ComponentDefinition
persistent identity: BBa E0022
display id: BBa_E0022
version: 1
was generated by: iGEM to SBOL conversion
description: enhanced cyan fluorescent protein derived from A. victoria GFP
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:25Z
mutable provenance: Modified from <bb_part>BBa_E0021</bb_part>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Cyan fluorescent protein (ECFP) reporter coding sequence without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission.
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_E0022 cyan fluorescent protein is based on BioBrick part BBa_E0021. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: BBa_E0022
sequence annotation: CFP (LVA)
sequence annotation: A
sequence annotation: SsrA
sequence annotation: 2
sequence: https://synbiohub.org/public/igem/BBa_E0022_sequence/1

BBa_E0022
type: SequenceAnnotation
persistent identity: annotation7040
display id: annotation7040
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation7040/range7040/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_E0022/annotation7040/range7040/1
type: Range
persistent identity: range7040
display id: range7040
version: 1
top level: ECFP
direction: direction/forward
start: 1
end: 762
orientation: inline

CFP (LVA)
type: SequenceAnnotation
persistent identity: annotation2150
display id: annotation2150
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2150/range2150/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E0022/annotation2150/range2150/1
type: Range
persistent identity: range2150
display id: range2150
version: 1
top level: ECFP
direction: direction/forward
start: 1
end: 762
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation2155
display id: annotation2155
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2155/range2155/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_E0022/annotation2155/range2155/1
type: Range
persistent identity: range2155
display id: range2155
version: 1
top level: ECFP
direction: direction/forward
start: 69
end: 69
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation2153
display id: annotation2153
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2153/range2153/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_E0022/annotation2153/range2153/1
type: Range
persistent identity: range2153
display id: range2153
version: 1
top level: ECFP
direction: direction/forward
start: 719
end: 756
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation2154
display id: annotation2154
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2154/range2154/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_E0022/annotation2154/range2154/1
type: Range
persistent identity: range2154
display id: range2154
version: 1
top level: ECFP
direction: direction/forward
start: 757
end: 762
orientation: inline

https://synbiohub.org/public/igem/BBa_I12230_sequence/1
type: Sequence
persistent identity: BBa I12230 sequence
display id: BBa_I12230_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aataaacttgactaaagattcctttagtagataatttaagtgttctttaatttctactagagtcacacaggaaagtactagatgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaataaacttgactaaagattcctttagtagataatttaagtgttctttaatttctactagagtcacacaggaaagtactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcata…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I12220_sequence/1
type: Sequence
persistent identity: BBa I12220 sequence
display id: BBa_I12220_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaagtactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgaatcaaggctagaaagactggaacagctatttctactgatttttcctcgagaagaccttgacatgattttgaaaatggattctttacaggatataaaagcattgttaacaggattattttaataaccctgatagtgctagtgtagatccctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I12221_sequence/1
type: Sequence
persistent identity: BBa I12221 sequence
display id: BBa_I12221_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaagtactagatgcagggttctgtgacagagtttctaaaaccgcgcctggttgatatcgagcaagtgagttcgacgcacgccaaggtgacccttgagcctttagagcgtggctttggccatactctgggtaacgcactgcgccgtattctgctctcatcgatgccgggttgcgcggtgaccgaggttgagattgatggtgtactacatgagtacagcaccaaagaaggcgttcaggaagatatcctggaaatcctgctcaacctgaaagggctggcggtgagagttcagggcaaagatgaagttattcttaccttgaataaatctggcattggccctgtgacggcagccgatatcacccacgacggtgatgtcgaaatcgtcaagccgcagcacgtgatctgccacctgaccgatgagaacgcgtctattagcatgcgtatcaaagttcagcgcggtcgtggttatgtgccggcttctacccgaatccattcggaagaagatgagcgcccaatcggccgtctgctggtcgacgcatgctacagccctgtggagcgtattgcctacaatgttgaagcagcgcgtgtagaacagcgtaccgacctggacaagctggtcatcgaaatggaaaccaacggcacaatcgatcctgaagaggcgattcgtcgtgcggcaaccattctggctgaacaactggaagctttcgttgacttacgtgatgtacgtcagcctgaagtgaaagaagagaaaccagagcctcaacagcagcaaatgcaacctcccaattcaagtgcgaacaacaaccctttgcaacagcaatcatcacaaaataccgtaccaaacgtcctcaaccaaattaaccaaatcttttctccagaggagcaacgcagcttattacaagaagccatcgaaacctgcaagaattttgaaaaaacacaattgggtagtacgatgacggaacctgtcaagcaaagttttattaggaaatacattaatcaaaaggccctgagaaaaatccaagctttgtaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I12222_sequence/1
type: Sequence
persistent identity: BBa I12222 sequence
display id: BBa_I12222_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaagtactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I12224_sequence/1
type: Sequence
persistent identity: BBa I12224 sequence
display id: BBa_I12224_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaagtactagatgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0011_sequence/1
type: Sequence
persistent identity: BBa R0011 sequence
display id: BBa_R0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0422_sequence/1
type: Sequence
persistent identity: BBa E0422 sequence
display id: BBa_E0422_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0053_sequence/1
type: Sequence
persistent identity: BBa R0053 sequence
display id: BBa_R0053_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aataaacttgactaaagattcctttagtagataatttaagtgttctttaatttc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I12210_sequence/1
type: Sequence
persistent identity: BBa I12210 sequence
display id: BBa_I12210_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caacacgcacggtgttaaggttctgttaagtaactttacactttatgcttccggctcgtatgttgtgtgg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I12200_sequence/1
type: Sequence
persistent identity: BBa I12200 sequence
display id: BBa_I12200_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgaatcaaggctagaaagactggaacagctatttctactgatttttcctcgagaagaccttgacatgattttgaaaatggattctttacaggatataaaagcattgttaacaggattattttaataaccctgatagtgctagtgtagatccc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I12201_sequence/1
type: Sequence
persistent identity: BBa I12201 sequence
display id: BBa_I12201_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcagggttctgtgacagagtttctaaaaccgcgcctggttgatatcgagcaagtgagttcgacgcacgccaaggtgacccttgagcctttagagcgtggctttggccatactctgggtaacgcactgcgccgtattctgctctcatcgatgccgggttgcgcggtgaccgaggttgagattgatggtgtactacatgagtacagcaccaaagaaggcgttcaggaagatatcctggaaatcctgctcaacctgaaagggctggcggtgagagttcagggcaaagatgaagttattcttaccttgaataaatctggcattggccctgtgacggcagccgatatcacccacgacggtgatgtcgaaatcgtcaagccgcagcacgtgatctgccacctgaccgatgagaacgcgtctattagcatgcgtatcaaagttcagcgcggtcgtggttatgtgccggcttctacccgaatccattcggaagaagatgagcgcccaatcggccgtctgctggtcgacgcatgctacagccctgtggagcgtattgcctacaatgttgaagcagcgcgtgtagaacagcgtaccgacctggacaagctggtcatcgaaatggaaaccaacggcacaatcgatcctgaagaggcgattcgtcgtgcggcaaccattctggctgaacaactggaagctttcgttgacttacgtgatgtacgtcagcctgaagtgaaagaagagaaaccagagcctcaacagcagcaaatgcaacctcccaattcaagtgcgaacaacaaccctttgcaacagcaatcatcacaaaataccgtaccaaacgtcctcaaccaaattaaccaaatcttttctccagaggagcaacgcagcttattacaagaagccatcgaaacctgcaagaattttgaaaaaacacaattgggtagtacgatgacggaacctgtcaagcaaagttttattaggaaatacattaatcaaaaggccctgagaaaaatccaagctttgtaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I12202_sequence/1
type: Sequence
persistent identity: BBa I12202 sequence
display id: BBa_I12202_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0013_sequence/1
type: Sequence
persistent identity: BBa C0013 sequence
display id: BBa_C0013_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0022_sequence/1
type: Sequence
persistent identity: BBa E0022 sequence
display id: BBa_E0022_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z