LOCUS BBa_I12232 5307 bp DNA linear 22-Dec-2024 DEFINITION TetR heterodimer test construct ACCESSION BBa_I12232 VERSION BBa_I12232.1 FEATURES Location/Qualifiers misc_feature 1..3 /label=OR3 misc_feature 11..28 /label=OR2 misc_feature 34..51 /label=OR1 promoter 18..23 /label=-35 promoter 8..13 /label=-35 promoter 30..35 /label=-10 misc_feature 1..54 /label=BBa_R0053 promoter 1..54 /label=BBa_R0053 RBS 63..75 /label=RBS-3\Weak RBS 69..72 /label=RBS misc_feature 63..75 /label=BBa_B0032 RBS 63..75 /label=BBa_B0032 misc_feature 1168..1192 /label=Help:Barcodes CDS 82..1167 /label=BBa_C0013 stem_loop 1212..1255 /label=stem_loop misc_feature 1201..1280 /label=BBa_B0010 terminator 1201..1280 /label=BBa_B0010 stem_loop 1296..1315 /label=T7 TE misc_feature 1322..1322 /label=stop polyA_site 1316..1329 /label=polya misc_feature 1289..1329 /label=BBa_B0012 terminator 1289..1329 /label=BBa_B0012 misc_feature 63..1329 /label=BBa_I12224 misc_feature 1338..1340 /label=OR3 misc_feature 1348..1365 /label=OR2 misc_feature 1371..1388 /label=OR1 promoter 1355..1360 /label=-35 promoter 1345..1350 /label=-35 promoter 1367..1372 /label=-10 misc_feature 1338..1391 /label=BBa_R0053 promoter 1338..1391 /label=BBa_R0053 RBS 1400..1412 /label=RBS-3\Weak RBS 1406..1409 /label=RBS misc_feature 1400..1412 /label=BBa_B0032 RBS 1400..1412 /label=BBa_B0032 CDS 1419..2300 /label=araC misc_feature 2301..2325 /label=Help:Barcodes CDS 1419..2300 /label=BBa_I12205 stem_loop 2345..2388 /label=stem_loop misc_feature 2334..2413 /label=BBa_B0010 terminator 2334..2413 /label=BBa_B0010 stem_loop 2429..2448 /label=T7 TE misc_feature 2455..2455 /label=stop polyA_site 2449..2462 /label=polya misc_feature 2422..2462 /label=BBa_B0012 terminator 2422..2462 /label=BBa_B0012 misc_feature 1400..2462 /label=BBa_I12225 misc_feature 2473..2489 /label=lac O1 promoter 2490..2495 /label=-35 misc_feature 2496..2512 /label=lac O1 promoter 2513..2518 /label=-10 misc_feature 2471..2524 /label=BBa_R0011 promoter 2471..2524 /label=BBa_R0011 RBS 2534..2546 /label=RBS-3\Weak RBS 2540..2543 /label=RBS misc_feature 2534..2546 /label=BBa_B0032 RBS 2534..2546 /label=BBa_B0032 CDS 2553..3179 /label=tetR misc_feature 3180..3204 /label=Help:Barcodes CDS 2553..3179 /label=BBa_I12202 stem_loop 3224..3267 /label=stem_loop misc_feature 3213..3292 /label=BBa_B0010 terminator 3213..3292 /label=BBa_B0010 stem_loop 3308..3327 /label=T7 TE misc_feature 3334..3334 /label=stop polyA_site 3328..3341 /label=polya misc_feature 3301..3341 /label=BBa_B0012 terminator 3301..3341 /label=BBa_B0012 misc_feature 2534..3341 /label=BBa_I12222 misc_feature 3353..3378 /label=c-amp2 misc_feature 3355..3393 /label=araO1 misc_feature 3392..3421 /label=c-amp1 misc_feature 3422..3450 /label=ara1 and ara2 promoter 3462..3467 /label=-35 promoter 3485..3490 /label=-10 promoter 3350..3498 /label=BBa_R0080 misc_feature 3511..3514 /label=conserved RBS 3507..3518 /label=BBa_B0034 CDS 3525..4151 /label=tetR-4C misc_feature 4152..4176 /label=Help:Barcodes CDS 3525..4151 /label=BBa_I12203 stem_loop 4196..4239 /label=stem_loop misc_feature 4185..4264 /label=BBa_B0010 terminator 4185..4264 /label=BBa_B0010 stem_loop 4280..4299 /label=T7 TE misc_feature 4306..4306 /label=stop polyA_site 4300..4313 /label=polya misc_feature 4273..4313 /label=BBa_B0012 terminator 4273..4313 /label=BBa_B0012 misc_feature 3507..4313 /label=BBa_I12223 promoter 4322..4327 /label=-35 misc_feature 4323..4341 /label=TetR O2 promoter 4343..4348 /label=-35 promoter 4347..4351 /label=-10 misc_feature 4353..4371 /label=O1 promoter 4366..4371 /label=-10 misc_feature 4328..4328 /label=O2 mutation misc_feature 4358..4358 /label=O1 mutation promoter 4322..4382 /label=BBa_I12212 misc_feature 4395..4398 /label=conserved RBS 4391..4402 /label=BBa_B0034 CDS 4409..5170 /label=CFP (LVA) misc_feature 5127..5164 /label=SsrA misc_feature 5165..5170 /label=2 misc_feature 4477..4477 /label=A misc_feature 4409..5170 /label=BBa_E0022 misc_feature 4409..5170 /label=BBa_E0022 stem_loop 5190..5233 /label=stem_loop misc_feature 5179..5258 /label=BBa_B0010 terminator 5179..5258 /label=BBa_B0010 stem_loop 5274..5293 /label=T7 TE misc_feature 5300..5300 /label=stop polyA_site 5294..5307 /label=polya misc_feature 5267..5307 /label=BBa_B0012 terminator 5267..5307 /label=BBa_B0012 misc_feature 4391..5307 /label=BBa_E0422 ORIGIN 1 aataaacttg actaaagatt cctttagtag ataatttaag tgttctttaa tttctactag 61 agtcacacag gaaagtacta gatgaaacca gtaacgttat acgatgtcgc agagtatgcc 121 ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa 181 acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt acattcccaa ccgcgtggca 241 caacaactgg cgggcaaaca gtcgttgctg attggcgttg ccacctccag tctggccctg 301 cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc 361 gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat 421 cttctcgcgc aacgcgtcag tgggctgatc attaactatc cgctggatga ccaggatgcc 481 attgctgtgg aagctgcctg cactaatgtt ccggcgttat ttcttgatgt ctctgaccag 541 acacccatca acagtattat tttctcccat gaagacggta cgcgactggg cgtggagcat 601 ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg 661 gcgcgtctgc gtctggctgg ctggcataaa tatctcactc gcaatcaaat tcagccgata 721 gcggaacggg aaggcgactg gagtgccatg tccggttttc aacaaaccat gcaaatgctg 781 aatgagggca tcgttcccac tgcgatgctg gttgccaacg atcagatggc gctgggcgca 841 atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg atatctcggt agtgggatac 901 gacgataccg aagacagctc atgttatatc ccgccgttaa ccaccatcaa acaggatttt 961 cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg 1021 aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat 1081 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt 1141 tcccgactgg aaagcgggca gtaataacac tgatagtgct agtgtagatc actactagag 1201 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt 1261 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct 1321 gcgtttatat actagagaat aaacttgact aaagattcct ttagtagata atttaagtgt 1381 tctttaattt ctactagagt cacacaggaa agtactagat ggctgaagcg caaaatgatc 1441 ccctgctgcc gggatactcg tttaacgccc atctggtggc gggtttaacg ccgattgagg 1501 ccaacggtta tctcgatttt tttatcgacc gaccgctggg aatgaaaggt tatattctca 1561 atctcaccat tcgcggtcag ggggtggtga aaaatcaggg acgagaattt gtctgccgac 1621 cgggtgatat tttgctgttc ccgccaggag agattcatca ctacggtcgt catccggagg 1681 ctcgcgaatg gtatcaccag tgggtttact ttcgtccgcg cgcctactgg catgaatggc 1741 ttaactggcc gtcaatattt gccaatacgg gtttctttcg cccggatgaa gcgcaccagc 1801 cgcatttcag cgacctgttt gggcaaatca ttaacgccgg gcaaggggaa gggcgctatt 1861 cggagctgct ggcgataaat ctgcttgagc aattgttact gcggcgcatg gaagcgatta 1921 acgagtcgct ccatccaccg atggataatc gggtacgcga ggcttgtcag tacatcagcg 1981 atcacctggc agacagcaat tttgatatcg ccagcgtcgc acagcatgtt tgcttgtcgc 2041 cgtcgcgtct gtcacatctt ttccgccagc agttagggat tagcgtctta agctggcgcg 2101 aggaccaacg cattagtcag gcgaagctgc ttttgagcac tacccggatg cctatcgcca 2161 ccgtcggtcg caatgttggt tttgacgatc aactctattt ctcgcgagta tttaaaaaat 2221 gcaccggggc cagcccgagc gagtttcgtg ccggttgtga agaaaaagtg aatgatgtag 2281 ccgtcaagtt gtcataataa cgctgatagt gctagtgtag atcgctacta gagccaggca 2341 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc 2401 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta 2461 tatactagag aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga 2521 gcacatacta gagtcacaca ggaaagtact agatgtccag attagataaa agtaaagtga 2581 ttaacagcgc attagagctg cttaatgagg tcggaatcga aggtttaaca acccgtaaac 2641 tcgcccagaa gctaggtgta gagcagccta cattgtattg gcatgtaaaa aataagcggg 2701 ctttgctcga cgccttagcc attgagatgt tagataggca ccatactcac ttttgccctt 2761 tagaagggga aagctggcaa gattttttac gtaataacgc taaaagtttt agatgtgctt 2821 tactaagtca tcgcgatgga gcaaaagtac atttaggtac acggcctaca gaaaaacagt 2881 atgaaactct cgaaaatcaa ttagcctttt tatgccaaca aggtttttca ctagagaatg 2941 cattatatgc actcagcgct gtggggcatt ttactttagg ttgcgtattg gaagatcaag 3001 agcatcaagt cgctaaagaa gaaagggaaa cacctactac tgatagtatg ccgccattat 3061 tacgacaagc tatcgaatta tttgatcacc aaggtgcaga gccagccttc ttattcggcc 3121 ttgaattgat catatgcgga ttagaaaaac aacttaaatg tgaaagtggg tcttaataac 3181 tctgatagtg ctagtgtaga tctctactag agccaggcat caaataaaac gaaaggctca 3241 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag 3301 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagagg cgtaacaaaa 3361 gtgtctataa tcacggcaga aaagtccaca ttgattattt gcacggcgtc acactttgct 3421 atgccatagc atttttatcc ataagattag cggatcctac ctgacgcttt ttatcgcaac 3481 tctctactgt ttctccatta ctagagaaag aggagaaata ctagatgtcc agattagata 3541 aaagtaaagt gattaacagc gcattagagc tgcttaatga ggtcggaatc gaaggtttaa 3601 caacccgtaa actcgcccag aagctaggtg tagcgcagca aacattgatg tggcatgtaa 3661 aaaataagcg ggctttgctc gacgccttag ccattgagat gttagatagg caccatactc 3721 acttttgccc tttagaaggg gaaagctggc aagatttttt acgtaataac gctaaaagtt 3781 ttagatgtgc tttactaagt catcgcgatg gagcaaaagt acatttaggt acacggccta 3841 cagaaaaaca gtatgaaact ctcgaaaatc aattagcctt tttatgccaa caaggttttt 3901 cactagagaa tgcattatat gcactcagcg ctgtggggca ttttacttta ggttgcgtat 3961 tggaagatca agagcatcaa gtcgctaaag aagaaaggga aacacctact actgatagta 4021 tgccgccatt attacgacaa gctatcgaat tatttgatca ccaaggtgca gagccagcct 4081 tcttattcgg ccttgaattg atcatatgcg gattagaaaa acaacttaaa tgtgaaagtg 4141 ggtcttaata acactgatag tgctagtgta gatcactact agagccaggc atcaaataaa 4201 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc 4261 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga 4321 gttctctgtc actgataggg agtggtaaaa taactctgtc aatgatagag tggattcaaa 4381 aatactagag aaagaggaga aatactagat ggtgagcaag ggcgaggagc tgttcaccgg 4441 ggtggtgccc atcctggtcg agctggacgg cgacgtgaac ggccacaagt tcagcgtgtc 4501 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac 4561 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctggg gcgtgcagtg 4621 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga 4681 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc 4741 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt 4801 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacatca gccacaacgt 4861 ctatatcacc gccgacaagc agaagaacgg catcaaggcc aacttcaaga tccgccacaa 4921 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga 4981 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga 5041 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac 5101 tctcggcatg gacgagctgt acaagaggcc tgctgcaaac gacgaaaact acgctttagt 5161 agcttaataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg 5221 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac 5281 cttcgggtgg gcctttctgc gtttata //