BBa_I12232
type: ComponentDefinition
persistent identity: BBa I12232
display id: BBa_I12232
version: 1
was generated by: iGEM to SBOL conversion
description: TetR heterodimer test construct
created: 2004-08-06T11:00:00Z
modified: 2015-08-31T04:07:32Z
owned by: james
owned by: myers
mutable description: This is a testing construct for the TetR - TetR-4C heterodimerization system.
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 120
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: rwald
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_E0422
component: BBa_I12212
component: BBa_I12222
component: BBa_R0011
component: BBa_I12223
component: BBa_R0080
component: BBa_I12224
component: BBa_R0053
component: BBa_I12225
sequence annotation: BBa_R0053
sequence annotation: BBa_I12224
sequence annotation: BBa_I12225
sequence annotation: BBa_R0011
sequence annotation: BBa_I12222
sequence annotation: BBa_R0080
sequence annotation: BBa_I12223
sequence annotation: BBa_I12212
sequence annotation: BBa_E0422
sequence: https://synbiohub.org/public/igem/BBa_I12232_sequence/1

BBa_E0422
type: Component
persistent identity: component2239442
display id: component2239442
version: 1
top level: BBa_I12232
definition: BBa_E0422
access: public

BBa_I12212
type: Component
persistent identity: component2239428
display id: component2239428
version: 1
top level: BBa_I12232
definition: TetR-4C
access: public

BBa_I12222
type: Component
persistent identity: component2239400
display id: component2239400
version: 1
top level: BBa_I12232
definition: BBa_I12222
access: public

BBa_R0011
type: Component
persistent identity: component2239383
display id: component2239383
version: 1
top level: BBa_I12232
definition: lacI+pL
access: public

BBa_I12223
type: Component
persistent identity: component2239419
display id: component2239419
version: 1
top level: BBa_I12232
definition: BBa_I12223
access: public

BBa_R0080
type: Component
persistent identity: component2239407
display id: component2239407
version: 1
top level: BBa_I12232
definition: AraC
access: public

BBa_I12224
type: Component
persistent identity: component2239362
display id: component2239362
version: 1
top level: BBa_I12232
definition: BBa_I12224
access: public

BBa_R0053
type: Component
persistent identity: component2239345
display id: component2239345
version: 1
top level: BBa_I12232
definition: cII p22
access: public

BBa_I12225
type: Component
persistent identity: component2239382
display id: component2239382
version: 1
top level: BBa_I12232
definition: BBa_I12225
access: public

BBa_R0053
type: Component
persistent identity: component2239364
display id: component2239364
version: 1
top level: BBa_I12232
definition: cII p22
access: public

BBa_R0053
type: SequenceAnnotation
persistent identity: annotation2239345
display id: annotation2239345
version: 1
top level: BBa_I12232
location: https://synbiohub.org/public/igem/BBa_I12232/annotation2239345/range2239345/1
component: BBa_R0053

https://synbiohub.org/public/igem/BBa_I12232/annotation2239345/range2239345/1
type: Range
persistent identity: range2239345
display id: range2239345
version: 1
top level: BBa_I12232
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_I12224
type: SequenceAnnotation
persistent identity: annotation2239362
display id: annotation2239362
version: 1
top level: BBa_I12232
location: https://synbiohub.org/public/igem/BBa_I12232/annotation2239362/range2239362/1
component: BBa_I12224

https://synbiohub.org/public/igem/BBa_I12232/annotation2239362/range2239362/1
type: Range
persistent identity: range2239362
display id: range2239362
version: 1
top level: BBa_I12232
direction: direction/reverse
start: 63
end: 1329
orientation: inline

BBa_R0053
type: SequenceAnnotation
persistent identity: annotation2239364
display id: annotation2239364
version: 1
top level: BBa_I12232
location: https://synbiohub.org/public/igem/BBa_I12232/annotation2239364/range2239364/1
component: BBa_R0053

https://synbiohub.org/public/igem/BBa_I12232/annotation2239364/range2239364/1
type: Range
persistent identity: range2239364
display id: range2239364
version: 1
top level: BBa_I12232
direction: direction/forward
start: 1338
end: 1391
orientation: inline

BBa_I12225
type: SequenceAnnotation
persistent identity: annotation2239382
display id: annotation2239382
version: 1
top level: BBa_I12232
location: https://synbiohub.org/public/igem/BBa_I12232/annotation2239382/range2239382/1
component: BBa_I12225

https://synbiohub.org/public/igem/BBa_I12232/annotation2239382/range2239382/1
type: Range
persistent identity: range2239382
display id: range2239382
version: 1
top level: BBa_I12232
direction: direction/reverse
start: 1400
end: 2462
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2239383
display id: annotation2239383
version: 1
top level: BBa_I12232
location: https://synbiohub.org/public/igem/BBa_I12232/annotation2239383/range2239383/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_I12232/annotation2239383/range2239383/1
type: Range
persistent identity: range2239383
display id: range2239383
version: 1
top level: BBa_I12232
direction: direction/forward
start: 2471
end: 2524
orientation: inline

BBa_I12222
type: SequenceAnnotation
persistent identity: annotation2239400
display id: annotation2239400
version: 1
top level: BBa_I12232
location: https://synbiohub.org/public/igem/BBa_I12232/annotation2239400/range2239400/1
component: BBa_I12222

https://synbiohub.org/public/igem/BBa_I12232/annotation2239400/range2239400/1
type: Range
persistent identity: range2239400
display id: range2239400
version: 1
top level: BBa_I12232
direction: direction/reverse
start: 2534
end: 3341
orientation: inline

BBa_R0080
type: SequenceAnnotation
persistent identity: annotation2239407
display id: annotation2239407
version: 1
top level: BBa_I12232
location: https://synbiohub.org/public/igem/BBa_I12232/annotation2239407/range2239407/1
component: BBa_R0080

https://synbiohub.org/public/igem/BBa_I12232/annotation2239407/range2239407/1
type: Range
persistent identity: range2239407
display id: range2239407
version: 1
top level: BBa_I12232
direction: direction/reverse
start: 3350
end: 3498
orientation: inline

BBa_I12223
type: SequenceAnnotation
persistent identity: annotation2239419
display id: annotation2239419
version: 1
top level: BBa_I12232
location: https://synbiohub.org/public/igem/BBa_I12232/annotation2239419/range2239419/1
component: BBa_I12223

https://synbiohub.org/public/igem/BBa_I12232/annotation2239419/range2239419/1
type: Range
persistent identity: range2239419
display id: range2239419
version: 1
top level: BBa_I12232
direction: direction/reverse
start: 3507
end: 4313
orientation: inline

BBa_I12212
type: SequenceAnnotation
persistent identity: annotation2239428
display id: annotation2239428
version: 1
top level: BBa_I12232
location: https://synbiohub.org/public/igem/BBa_I12232/annotation2239428/range2239428/1
component: BBa_I12212

https://synbiohub.org/public/igem/BBa_I12232/annotation2239428/range2239428/1
type: Range
persistent identity: range2239428
display id: range2239428
version: 1
top level: BBa_I12232
direction: direction/reverse
start: 4322
end: 4382
orientation: inline

BBa_E0422
type: SequenceAnnotation
persistent identity: annotation2239442
display id: annotation2239442
version: 1
top level: BBa_I12232
location: https://synbiohub.org/public/igem/BBa_I12232/annotation2239442/range2239442/1
component: BBa_E0422

https://synbiohub.org/public/igem/BBa_I12232/annotation2239442/range2239442/1
type: Range
persistent identity: range2239442
display id: range2239442
version: 1
top level: BBa_I12232
direction: direction/reverse
start: 4391
end: 5307
orientation: inline

TetR-4C
type: ComponentDefinition
persistent identity: BBa I12212
display id: BBa_I12212
version: 1
was generated by: iGEM to SBOL conversion
description: TetR - TetR-4C heterodimer promoter (negative)
created: 2004-08-05T11:00:00Z
modified: 2015-08-31T04:07:31Z
owned by: james
owned by: myers
mutable description: This promoter is repressed by a heterodimer of wild-type TetR and mutant TetR-4C. It differs from wild-type TetR promoter in that the +4 and -4 regions of its O2 binding site have been changed, and the -35 region of the reverse promoter (pA) has been destroyed.
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 120
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The O2 and O1 mutations are all single point mutations, occuring at the first base which appears listed here.
star: false
creator: rwald
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: -35
sequence annotation: TetR O2
sequence annotation: O2 mutation
sequence annotation: -10
sequence annotation: O1
sequence annotation: O1 mutation
sequence: https://synbiohub.org/public/igem/BBa_I12212_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation1031337
display id: annotation1031337
version: 1
top level: TetR-4C
location: https://synbiohub.org/public/igem/BBa_I12212/annotation1031337/range1031337/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_I12212/annotation1031337/range1031337/1
type: Range
persistent identity: range1031337
display id: range1031337
version: 1
top level: TetR-4C
direction: direction/forward
start: 1
end: 6
orientation: inline

TetR O2
type: SequenceAnnotation
persistent identity: annotation1031338
display id: annotation1031338
version: 1
top level: TetR-4C
location: https://synbiohub.org/public/igem/BBa_I12212/annotation1031338/range1031338/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_I12212/annotation1031338/range1031338/1
type: Range
persistent identity: range1031338
display id: range1031338
version: 1
top level: TetR-4C
direction: direction/forward
start: 2
end: 20
orientation: inline

O2 mutation
type: SequenceAnnotation
persistent identity: annotation1057573
display id: annotation1057573
version: 1
top level: TetR-4C
location: https://synbiohub.org/public/igem/BBa_I12212/annotation1057573/range1057573/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_I12212/annotation1057573/range1057573/1
type: Range
persistent identity: range1057573
display id: range1057573
version: 1
top level: TetR-4C
direction: direction/forward
start: 7
end: 7
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1031339
display id: annotation1031339
version: 1
top level: TetR-4C
location: https://synbiohub.org/public/igem/BBa_I12212/annotation1031339/range1031339/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I12212/annotation1031339/range1031339/1
type: Range
persistent identity: range1031339
display id: range1031339
version: 1
top level: TetR-4C
direction: direction/forward
start: 22
end: 27
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1031340
display id: annotation1031340
version: 1
top level: TetR-4C
location: https://synbiohub.org/public/igem/BBa_I12212/annotation1031340/range1031340/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I12212/annotation1031340/range1031340/1
type: Range
persistent identity: range1031340
display id: range1031340
version: 1
top level: TetR-4C
direction: direction/forward
start: 26
end: 30
orientation: inline

O1
type: SequenceAnnotation
persistent identity: annotation1031341
display id: annotation1031341
version: 1
top level: TetR-4C
location: https://synbiohub.org/public/igem/BBa_I12212/annotation1031341/range1031341/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_I12212/annotation1031341/range1031341/1
type: Range
persistent identity: range1031341
display id: range1031341
version: 1
top level: TetR-4C
direction: direction/forward
start: 32
end: 50
orientation: inline

O1 mutation
type: SequenceAnnotation
persistent identity: annotation1057574
display id: annotation1057574
version: 1
top level: TetR-4C
location: https://synbiohub.org/public/igem/BBa_I12212/annotation1057574/range1057574/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_I12212/annotation1057574/range1057574/1
type: Range
persistent identity: range1057574
display id: range1057574
version: 1
top level: TetR-4C
direction: direction/forward
start: 37
end: 37
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1031342
display id: annotation1031342
version: 1
top level: TetR-4C
location: https://synbiohub.org/public/igem/BBa_I12212/annotation1031342/range1031342/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I12212/annotation1031342/range1031342/1
type: Range
persistent identity: range1031342
display id: range1031342
version: 1
top level: TetR-4C
direction: direction/forward
start: 45
end: 50
orientation: inline

BBa_I12222
type: ComponentDefinition
persistent identity: BBa I12222
display id: BBa_I12222
version: 1
was generated by: iGEM to SBOL conversion
description: Wild-type TetR(B) protein generator
created: 2004-08-06T11:00:00Z
modified: 2015-08-31T04:07:32Z
owned by: james
owned by: myers
mutable description: Protein generator (with RBS, CDS, and terminators) for Wild-type TetR(B) (BBa_I12202).
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 120
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: rwald
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0012
component: BBa_B0010
component: BBa_I12202
component: BBa_B0032
sequence annotation: BBa_B0032
sequence annotation: BBa_I12202
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I12222_sequence/1

BBa_B0012
type: Component
persistent identity: component1041890
display id: component1041890
version: 1
top level: BBa_I12222
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1041880
display id: component1041880
version: 1
top level: BBa_I12222
definition: BBa_B0010
access: public

BBa_I12202
type: Component
persistent identity: component1041874
display id: component1041874
version: 1
top level: BBa_I12222
definition: TetR(B)
access: public

BBa_B0032
type: Component
persistent identity: component1041866
display id: component1041866
version: 1
top level: BBa_I12222
definition: BBa_B0032
access: public

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1041866
display id: annotation1041866
version: 1
top level: BBa_I12222
location: https://synbiohub.org/public/igem/BBa_I12222/annotation1041866/range1041866/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_I12222/annotation1041866/range1041866/1
type: Range
persistent identity: range1041866
display id: range1041866
version: 1
top level: BBa_I12222
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_I12202
type: SequenceAnnotation
persistent identity: annotation1041874
display id: annotation1041874
version: 1
top level: BBa_I12222
location: https://synbiohub.org/public/igem/BBa_I12222/annotation1041874/range1041874/1
component: BBa_I12202

https://synbiohub.org/public/igem/BBa_I12222/annotation1041874/range1041874/1
type: Range
persistent identity: range1041874
display id: range1041874
version: 1
top level: BBa_I12222
direction: direction/forward
start: 20
end: 646
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1041880
display id: annotation1041880
version: 1
top level: BBa_I12222
location: https://synbiohub.org/public/igem/BBa_I12222/annotation1041880/range1041880/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I12222/annotation1041880/range1041880/1
type: Range
persistent identity: range1041880
display id: range1041880
version: 1
top level: BBa_I12222
direction: direction/forward
start: 680
end: 759
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1041890
display id: annotation1041890
version: 1
top level: BBa_I12222
location: https://synbiohub.org/public/igem/BBa_I12222/annotation1041890/range1041890/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I12222/annotation1041890/range1041890/1
type: Range
persistent identity: range1041890
display id: range1041890
version: 1
top level: BBa_I12222
direction: direction/forward
start: 768
end: 808
orientation: inline

BBa_I12223
type: ComponentDefinition
persistent identity: BBa I12223
display id: BBa_I12223
version: 1
was generated by: iGEM to SBOL conversion
description: TetR-4C protein generator
created: 2004-08-06T11:00:00Z
modified: 2015-08-31T04:07:32Z
owned by: james
owned by: myers
mutable description: Protein generator (with RBS, CDS, and terminators) for TetR-4C (BBa_I12203).
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 120
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: rwald
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_I12203
component: BBa_B0034
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_I12203
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I12223_sequence/1

BBa_I12203
type: Component
persistent identity: component1042142
display id: component1042142
version: 1
top level: BBa_I12223
definition: Tetr-4C
access: public

BBa_B0034
type: Component
persistent identity: component1042135
display id: component1042135
version: 1
top level: BBa_I12223
definition: BBa_B0034
access: public

BBa_B0012
type: Component
persistent identity: component1042158
display id: component1042158
version: 1
top level: BBa_I12223
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1042148
display id: component1042148
version: 1
top level: BBa_I12223
definition: BBa_B0010
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1042135
display id: annotation1042135
version: 1
top level: BBa_I12223
location: https://synbiohub.org/public/igem/BBa_I12223/annotation1042135/range1042135/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I12223/annotation1042135/range1042135/1
type: Range
persistent identity: range1042135
display id: range1042135
version: 1
top level: BBa_I12223
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_I12203
type: SequenceAnnotation
persistent identity: annotation1042142
display id: annotation1042142
version: 1
top level: BBa_I12223
location: https://synbiohub.org/public/igem/BBa_I12223/annotation1042142/range1042142/1
component: BBa_I12203

https://synbiohub.org/public/igem/BBa_I12223/annotation1042142/range1042142/1
type: Range
persistent identity: range1042142
display id: range1042142
version: 1
top level: BBa_I12223
direction: direction/forward
start: 19
end: 645
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1042148
display id: annotation1042148
version: 1
top level: BBa_I12223
location: https://synbiohub.org/public/igem/BBa_I12223/annotation1042148/range1042148/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I12223/annotation1042148/range1042148/1
type: Range
persistent identity: range1042148
display id: range1042148
version: 1
top level: BBa_I12223
direction: direction/forward
start: 679
end: 758
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1042158
display id: annotation1042158
version: 1
top level: BBa_I12223
location: https://synbiohub.org/public/igem/BBa_I12223/annotation1042158/range1042158/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I12223/annotation1042158/range1042158/1
type: Range
persistent identity: range1042158
display id: range1042158
version: 1
top level: BBa_I12223
direction: direction/forward
start: 767
end: 807
orientation: inline

BBa_I12224
type: ComponentDefinition
persistent identity: BBa I12224
display id: BBa_I12224
version: 1
was generated by: iGEM to SBOL conversion
description: LacI protein generator (LVA-)
created: 2004-08-06T11:00:00Z
modified: 2015-08-31T04:07:32Z
owned by: james
owned by: myers
mutable description: Protein generator for LacI (LVA-).
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 120
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: rwald
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0012
component: BBa_B0010
component: BBa_C0013
component: BBa_B0032
sequence annotation: BBa_B0032
sequence annotation: BBa_C0013
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I12224_sequence/1

BBa_B0012
type: Component
persistent identity: component1041957
display id: component1041957
version: 1
top level: BBa_I12224
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1041947
display id: component1041947
version: 1
top level: BBa_I12224
definition: BBa_B0010
access: public

BBa_C0013
type: Component
persistent identity: component1041941
display id: component1041941
version: 1
top level: BBa_I12224
definition: lacI
access: public

BBa_B0032
type: Component
persistent identity: component1041936
display id: component1041936
version: 1
top level: BBa_I12224
definition: BBa_B0032
access: public

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1041936
display id: annotation1041936
version: 1
top level: BBa_I12224
location: https://synbiohub.org/public/igem/BBa_I12224/annotation1041936/range1041936/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_I12224/annotation1041936/range1041936/1
type: Range
persistent identity: range1041936
display id: range1041936
version: 1
top level: BBa_I12224
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_C0013
type: SequenceAnnotation
persistent identity: annotation1041941
display id: annotation1041941
version: 1
top level: BBa_I12224
location: https://synbiohub.org/public/igem/BBa_I12224/annotation1041941/range1041941/1
component: BBa_C0013

https://synbiohub.org/public/igem/BBa_I12224/annotation1041941/range1041941/1
type: Range
persistent identity: range1041941
display id: range1041941
version: 1
top level: BBa_I12224
direction: direction/forward
start: 20
end: 1105
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1041947
display id: annotation1041947
version: 1
top level: BBa_I12224
location: https://synbiohub.org/public/igem/BBa_I12224/annotation1041947/range1041947/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I12224/annotation1041947/range1041947/1
type: Range
persistent identity: range1041947
display id: range1041947
version: 1
top level: BBa_I12224
direction: direction/forward
start: 1139
end: 1218
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1041957
display id: annotation1041957
version: 1
top level: BBa_I12224
location: https://synbiohub.org/public/igem/BBa_I12224/annotation1041957/range1041957/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I12224/annotation1041957/range1041957/1
type: Range
persistent identity: range1041957
display id: range1041957
version: 1
top level: BBa_I12224
direction: direction/forward
start: 1227
end: 1267
orientation: inline

BBa_I12225
type: ComponentDefinition
persistent identity: BBa I12225
display id: BBa_I12225
version: 1
was generated by: iGEM to SBOL conversion
description: AraC protein generator (LVA-)
created: 2004-08-06T11:00:00Z
modified: 2015-08-31T04:07:32Z
owned by: james
owned by: myers
mutable description: Protein generator for AraC.
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 120
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: rwald
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_I12205
component: BBa_B0032
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0032
sequence annotation: BBa_I12205
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I12225_sequence/1

BBa_I12205
type: Component
persistent identity: component1041976
display id: component1041976
version: 1
top level: BBa_I12225
definition: AraC
access: public

BBa_B0032
type: Component
persistent identity: component1041968
display id: component1041968
version: 1
top level: BBa_I12225
definition: BBa_B0032
access: public

BBa_B0012
type: Component
persistent identity: component1041992
display id: component1041992
version: 1
top level: BBa_I12225
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1041982
display id: component1041982
version: 1
top level: BBa_I12225
definition: BBa_B0010
access: public

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1041968
display id: annotation1041968
version: 1
top level: BBa_I12225
location: https://synbiohub.org/public/igem/BBa_I12225/annotation1041968/range1041968/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_I12225/annotation1041968/range1041968/1
type: Range
persistent identity: range1041968
display id: range1041968
version: 1
top level: BBa_I12225
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_I12205
type: SequenceAnnotation
persistent identity: annotation1041976
display id: annotation1041976
version: 1
top level: BBa_I12225
location: https://synbiohub.org/public/igem/BBa_I12225/annotation1041976/range1041976/1
component: BBa_I12205

https://synbiohub.org/public/igem/BBa_I12225/annotation1041976/range1041976/1
type: Range
persistent identity: range1041976
display id: range1041976
version: 1
top level: BBa_I12225
direction: direction/forward
start: 20
end: 901
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1041982
display id: annotation1041982
version: 1
top level: BBa_I12225
location: https://synbiohub.org/public/igem/BBa_I12225/annotation1041982/range1041982/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I12225/annotation1041982/range1041982/1
type: Range
persistent identity: range1041982
display id: range1041982
version: 1
top level: BBa_I12225
direction: direction/forward
start: 935
end: 1014
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1041992
display id: annotation1041992
version: 1
top level: BBa_I12225
location: https://synbiohub.org/public/igem/BBa_I12225/annotation1041992/range1041992/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I12225/annotation1041992/range1041992/1
type: Range
persistent identity: range1041992
display id: range1041992
version: 1
top level: BBa_I12225
direction: direction/forward
start: 1023
end: 1063
orientation: inline

lacI+pL
type: ComponentDefinition
persistent identity: BBa R0011
display id: BBa_R0011
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (lacI regulated, lambda pL hybrid)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site. LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
star: true
creator: Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0011
sequence annotation: lac O1
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0011_sequence/1

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation7064
display id: annotation7064
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
type: Range
persistent identity: range7064
display id: range7064
version: 1
top level: lacI+pL
direction: direction/forward
start: 1
end: 54
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation1999
display id: annotation1999
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
type: Range
persistent identity: range1999
display id: range1999
version: 1
top level: lacI+pL
direction: direction/forward
start: 3
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2000
display id: annotation2000
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
type: Range
persistent identity: range2000
display id: range2000
version: 1
top level: lacI+pL
direction: direction/forward
start: 20
end: 25
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation2001
display id: annotation2001
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
type: Range
persistent identity: range2001
display id: range2001
version: 1
top level: lacI+pL
direction: direction/forward
start: 26
end: 42
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2002
display id: annotation2002
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
type: Range
persistent identity: range2002
display id: range2002
version: 1
top level: lacI+pL
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_E0422
type: ComponentDefinition
persistent identity: BBa E0422
display id: BBa_E0422
version: 1
was generated by: iGEM to SBOL conversion
description: ECFP (RBS+ LVA+ TERM) (B0034.E0022.B0015)
created: 2003-12-04T12:00:00Z
modified: 2015-08-31T04:07:26Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: -- No description --
discontinued: false
dominant: true
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Caitlin Conboy and Jennifer Braff
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0034
component: BBa_B0010
component: BBa_E0022
component: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_E0022
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_E0422_sequence/1

BBa_B0034
type: Component
persistent identity: component942803
display id: component942803
version: 1
top level: BBa_E0422
definition: BBa_B0034
access: public

BBa_B0010
type: Component
persistent identity: component942823
display id: component942823
version: 1
top level: BBa_E0422
definition: BBa_B0010
access: public

BBa_E0022
type: Component
persistent identity: component942815
display id: component942815
version: 1
top level: BBa_E0422
definition: ECFP
access: public

BBa_B0012
type: Component
persistent identity: component942833
display id: component942833
version: 1
top level: BBa_E0422
definition: BBa_B0012
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation942803
display id: annotation942803
version: 1
top level: BBa_E0422
location: https://synbiohub.org/public/igem/BBa_E0422/annotation942803/range942803/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_E0422/annotation942803/range942803/1
type: Range
persistent identity: range942803
display id: range942803
version: 1
top level: BBa_E0422
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_E0022
type: SequenceAnnotation
persistent identity: annotation942815
display id: annotation942815
version: 1
top level: BBa_E0422
location: https://synbiohub.org/public/igem/BBa_E0422/annotation942815/range942815/1
component: BBa_E0022

https://synbiohub.org/public/igem/BBa_E0422/annotation942815/range942815/1
type: Range
persistent identity: range942815
display id: range942815
version: 1
top level: BBa_E0422
direction: direction/forward
start: 19
end: 780
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation942823
display id: annotation942823
version: 1
top level: BBa_E0422
location: https://synbiohub.org/public/igem/BBa_E0422/annotation942823/range942823/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_E0422/annotation942823/range942823/1
type: Range
persistent identity: range942823
display id: range942823
version: 1
top level: BBa_E0422
direction: direction/forward
start: 789
end: 868
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation942833
display id: annotation942833
version: 1
top level: BBa_E0422
location: https://synbiohub.org/public/igem/BBa_E0422/annotation942833/range942833/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_E0422/annotation942833/range942833/1
type: Range
persistent identity: range942833
display id: range942833
version: 1
top level: BBa_E0422
direction: direction/forward
start: 877
end: 917
orientation: inline

cII p22
type: ComponentDefinition
persistent identity: BBa R0053
display id: BBa_R0053
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (p22 cII regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Bacteriophage p22.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The p22 cII regulatory region sequence is a 97 base-pair sequence with the standard BioBrick prefix and suffix sections on its ends. p22 cII repressor protein, BBa_C0053, binds to it. This segment contains O-R1, O-R2, a fragment of O-R3, the -35 of P-RM, and P-R (-10 and -35 from Tom Knight)
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Maia Mahoney
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0053
sequence annotation: OR3
sequence annotation: -35
sequence annotation: OR2
sequence annotation: -10
sequence annotation: OR1
sequence: https://synbiohub.org/public/igem/BBa_R0053_sequence/1

BBa_R0053
type: SequenceAnnotation
persistent identity: annotation7069
display id: annotation7069
version: 1
top level: cII p22
location: https://synbiohub.org/public/igem/BBa_R0053/annotation7069/range7069/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0053/annotation7069/range7069/1
type: Range
persistent identity: range7069
display id: range7069
version: 1
top level: cII p22
direction: direction/forward
start: 1
end: 54
orientation: inline

OR3
type: SequenceAnnotation
persistent identity: annotation2035
display id: annotation2035
version: 1
top level: cII p22
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2035/range2035/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0053/annotation2035/range2035/1
type: Range
persistent identity: range2035
display id: range2035
version: 1
top level: cII p22
direction: direction/forward
start: 1
end: 3
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2041
display id: annotation2041
version: 1
top level: cII p22
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2041/range2041/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0053/annotation2041/range2041/1
type: Range
persistent identity: range2041
display id: range2041
version: 1
top level: cII p22
direction: direction/forward
start: 8
end: 13
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2036
display id: annotation2036
version: 1
top level: cII p22
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2036/range2036/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0053/annotation2036/range2036/1
type: Range
persistent identity: range2036
display id: range2036
version: 1
top level: cII p22
direction: direction/forward
start: 11
end: 28
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2038
display id: annotation2038
version: 1
top level: cII p22
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2038/range2038/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0053/annotation2038/range2038/1
type: Range
persistent identity: range2038
display id: range2038
version: 1
top level: cII p22
direction: direction/forward
start: 18
end: 23
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2042
display id: annotation2042
version: 1
top level: cII p22
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2042/range2042/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0053/annotation2042/range2042/1
type: Range
persistent identity: range2042
display id: range2042
version: 1
top level: cII p22
direction: direction/forward
start: 30
end: 35
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation2037
display id: annotation2037
version: 1
top level: cII p22
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2037/range2037/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0053/annotation2037/range2037/1
type: Range
persistent identity: range2037
display id: range2037
version: 1
top level: cII p22
direction: direction/forward
start: 34
end: 51
orientation: inline

AraC
type: ComponentDefinition
persistent identity: BBa R0080
display id: BBa_R0080
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (AraC regulated)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: GenBank: J01641 (www.ncbi.nlm.nih.gov)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: AraC operator, truncated to include araO1, araI1, araI2, c-amp1, and c-amp2 sites. This operator should *activate* transcription in the presence of AraC; b/c the operator lacks the araO2 site, there should not be araC-mediated repression.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Sara Neves (Fighting Darwins)
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: c-amp2
sequence annotation: araO1
sequence annotation: c-amp1
sequence annotation: ara1 and ara2
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0080_sequence/1

c-amp2
type: SequenceAnnotation
persistent identity: annotation301456
display id: annotation301456
version: 1
top level: AraC
location: https://synbiohub.org/public/igem/BBa_R0080/annotation301456/range301456/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0080/annotation301456/range301456/1
type: Range
persistent identity: range301456
display id: range301456
version: 1
top level: AraC
direction: direction/forward
start: 4
end: 29
orientation: inline

araO1
type: SequenceAnnotation
persistent identity: annotation301457
display id: annotation301457
version: 1
top level: AraC
location: https://synbiohub.org/public/igem/BBa_R0080/annotation301457/range301457/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0080/annotation301457/range301457/1
type: Range
persistent identity: range301457
display id: range301457
version: 1
top level: AraC
direction: direction/forward
start: 6
end: 44
orientation: inline

c-amp1
type: SequenceAnnotation
persistent identity: annotation301458
display id: annotation301458
version: 1
top level: AraC
location: https://synbiohub.org/public/igem/BBa_R0080/annotation301458/range301458/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0080/annotation301458/range301458/1
type: Range
persistent identity: range301458
display id: range301458
version: 1
top level: AraC
direction: direction/forward
start: 43
end: 72
orientation: inline

ara1 and ara2
type: SequenceAnnotation
persistent identity: annotation301462
display id: annotation301462
version: 1
top level: AraC
location: https://synbiohub.org/public/igem/BBa_R0080/annotation301462/range301462/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0080/annotation301462/range301462/1
type: Range
persistent identity: range301462
display id: range301462
version: 1
top level: AraC
direction: direction/forward
start: 73
end: 101
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation308601
display id: annotation308601
version: 1
top level: AraC
location: https://synbiohub.org/public/igem/BBa_R0080/annotation308601/range308601/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0080/annotation308601/range308601/1
type: Range
persistent identity: range308601
display id: range308601
version: 1
top level: AraC
direction: direction/forward
start: 113
end: 118
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation308602
display id: annotation308602
version: 1
top level: AraC
location: https://synbiohub.org/public/igem/BBa_R0080/annotation308602/range308602/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0080/annotation308602/range308602/1
type: Range
persistent identity: range308602
display id: range308602
version: 1
top level: AraC
direction: direction/forward
start: 136
end: 141
orientation: inline

TetR(B)
type: ComponentDefinition
persistent identity: BBa I12202
display id: BBa_I12202
version: 1
was generated by: iGEM to SBOL conversion
description: Wild-type TetR(B) (-LVA)
created: 2004-08-05T11:00:00Z
modified: 2015-08-31T04:07:31Z
mutable provenance: Serratia marcescens
owned by: james
owned by: myers
mutable description: This is the standard wild-type TetR repressor. It may appear that this is a duplicate of BBa_C0041, but that part has an error near the end which breaks its stop codon. This was presumably to facilitate addition of an LVA tag; nonetheless, for the stand-alone protein, a stop codon is needed.
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 120
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: rwald
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: tetR
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_I12202_sequence/1

tetR
type: SequenceAnnotation
persistent identity: annotation1031374
display id: annotation1031374
version: 1
top level: TetR(B)
location: https://synbiohub.org/public/igem/BBa_I12202/annotation1031374/range1031374/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I12202/annotation1031374/range1031374/1
type: Range
persistent identity: range1031374
display id: range1031374
version: 1
top level: TetR(B)
direction: direction/forward
start: 1
end: 627
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214021
display id: annotation2214021
version: 1
top level: TetR(B)
location: https://synbiohub.org/public/igem/BBa_I12202/annotation2214021/range2214021/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_I12202/annotation2214021/range2214021/1
type: Range
persistent identity: range2214021
display id: range2214021
version: 1
top level: TetR(B)
direction: direction/forward
start: 628
end: 652
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

Tetr-4C
type: ComponentDefinition
persistent identity: BBa I12203
display id: BBa_I12203
version: 1
was generated by: iGEM to SBOL conversion
description: TetR-4C
created: 2004-08-05T11:00:00Z
modified: 2015-08-31T04:07:31Z
owned by: james
owned by: myers
mutable description: This is a mutant form of TetR which binds to the tetO-4C operating region (rather than the standard tetO region). In particular, this is the EA37PQ39YM42 mutant.
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 120
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: rwald
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: tetR-4C
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_I12203_sequence/1

tetR-4C
type: SequenceAnnotation
persistent identity: annotation1031381
display id: annotation1031381
version: 1
top level: Tetr-4C
location: https://synbiohub.org/public/igem/BBa_I12203/annotation1031381/range1031381/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I12203/annotation1031381/range1031381/1
type: Range
persistent identity: range1031381
display id: range1031381
version: 1
top level: Tetr-4C
direction: direction/forward
start: 1
end: 627
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214022
display id: annotation2214022
version: 1
top level: Tetr-4C
location: https://synbiohub.org/public/igem/BBa_I12203/annotation2214022/range2214022/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_I12203/annotation2214022/range2214022/1
type: Range
persistent identity: range2214022
display id: range2214022
version: 1
top level: Tetr-4C
direction: direction/forward
start: 628
end: 652
orientation: inline

AraC
type: ComponentDefinition
persistent identity: BBa I12205
display id: BBa_I12205
version: 1
was generated by: iGEM to SBOL conversion
description: AraC
created: 2004-08-06T11:00:00Z
modified: 2015-08-31T04:07:31Z
owned by: james
owned by: myers
mutable description: The coding region for the AraC protein.
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 120
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: rwald
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: araC
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_I12205_sequence/1

araC
type: SequenceAnnotation
persistent identity: annotation1032104
display id: annotation1032104
version: 1
top level: AraC
location: https://synbiohub.org/public/igem/BBa_I12205/annotation1032104/range1032104/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I12205/annotation1032104/range1032104/1
type: Range
persistent identity: range1032104
display id: range1032104
version: 1
top level: AraC
direction: direction/forward
start: 1
end: 882
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214023
display id: annotation2214023
version: 1
top level: AraC
location: https://synbiohub.org/public/igem/BBa_I12205/annotation2214023/range2214023/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_I12205/annotation2214023/range2214023/1
type: Range
persistent identity: range2214023
display id: range2214023
version: 1
top level: AraC
direction: direction/forward
start: 883
end: 907
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

ECFP
type: ComponentDefinition
persistent identity: BBa E0022
display id: BBa_E0022
version: 1
was generated by: iGEM to SBOL conversion
description: enhanced cyan fluorescent protein derived from A. victoria GFP
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:25Z
mutable provenance: Modified from <bb_part>BBa_E0021</bb_part>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Cyan fluorescent protein (ECFP) reporter coding sequence without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission.
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_E0022 cyan fluorescent protein is based on BioBrick part BBa_E0021. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: BBa_E0022
sequence annotation: CFP (LVA)
sequence annotation: A
sequence annotation: SsrA
sequence annotation: 2
sequence: https://synbiohub.org/public/igem/BBa_E0022_sequence/1

BBa_E0022
type: SequenceAnnotation
persistent identity: annotation7040
display id: annotation7040
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation7040/range7040/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_E0022/annotation7040/range7040/1
type: Range
persistent identity: range7040
display id: range7040
version: 1
top level: ECFP
direction: direction/forward
start: 1
end: 762
orientation: inline

CFP (LVA)
type: SequenceAnnotation
persistent identity: annotation2150
display id: annotation2150
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2150/range2150/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E0022/annotation2150/range2150/1
type: Range
persistent identity: range2150
display id: range2150
version: 1
top level: ECFP
direction: direction/forward
start: 1
end: 762
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation2155
display id: annotation2155
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2155/range2155/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_E0022/annotation2155/range2155/1
type: Range
persistent identity: range2155
display id: range2155
version: 1
top level: ECFP
direction: direction/forward
start: 69
end: 69
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation2153
display id: annotation2153
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2153/range2153/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_E0022/annotation2153/range2153/1
type: Range
persistent identity: range2153
display id: range2153
version: 1
top level: ECFP
direction: direction/forward
start: 719
end: 756
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation2154
display id: annotation2154
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2154/range2154/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_E0022/annotation2154/range2154/1
type: Range
persistent identity: range2154
display id: range2154
version: 1
top level: ECFP
direction: direction/forward
start: 757
end: 762
orientation: inline

lacI
type: ComponentDefinition
persistent identity: BBa C0013
display id: BBa_C0013
version: 1
was generated by: iGEM to SBOL conversion
description: Repressor, lacI
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: www.ncbi.nlm.nih.gov
owned by: james
owned by: myers
mutable description: LacI repressor protein, without SsrA degradation tag. No Barcode.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Yong-Su Jin (Fighting Darwins)
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0013_sequence/1

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214005
display id: annotation2214005
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0013/annotation2214005/range2214005/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0013/annotation2214005/range2214005/1
type: Range
persistent identity: range2214005
display id: range2214005
version: 1
top level: lacI
direction: direction/forward
start: 1087
end: 1111
orientation: inline

https://synbiohub.org/public/igem/BBa_I12232_sequence/1
type: Sequence
persistent identity: BBa I12232 sequence
display id: BBa_I12232_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aataaacttgactaaagattcctttagtagataatttaagtgttctttaatttctactagagtcacacaggaaagtactagatgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaataaacttgactaaagattcctttagtagataatttaagtgttctttaatttctactagagtcacacaggaaagtactagatggctgaagcgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggtttaacgccgattgaggccaacggttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctcaccattcgcggtcagggggtggtgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctgttcccgccaggagagattcatcactacggtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgcctactggcatgaatggcttaactggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagccgcatttcagcgacctgtttgggcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatctgcttgagcaattgttactgcggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgaggcttgtcagtacatcagcgatcacctggcagacagcaat…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I12212_sequence/1
type: Sequence
persistent identity: BBa I12212 sequence
display id: BBa_I12212_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttctctgtcactgatagggagtggtaaaataactctgtcaatgatagagtggattcaaaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I12222_sequence/1
type: Sequence
persistent identity: BBa I12222 sequence
display id: BBa_I12222_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaagtactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I12223_sequence/1
type: Sequence
persistent identity: BBa I12223 sequence
display id: BBa_I12223_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagcgcagcaaacattgatgtggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I12224_sequence/1
type: Sequence
persistent identity: BBa I12224 sequence
display id: BBa_I12224_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaagtactagatgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I12225_sequence/1
type: Sequence
persistent identity: BBa I12225 sequence
display id: BBa_I12225_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaagtactagatggctgaagcgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggtttaacgccgattgaggccaacggttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctcaccattcgcggtcagggggtggtgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctgttcccgccaggagagattcatcactacggtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgcctactggcatgaatggcttaactggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagccgcatttcagcgacctgtttgggcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatctgcttgagcaattgttactgcggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgaggcttgtcagtacatcagcgatcacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcttgtcgccgtcgcgtctgtcacatcttttccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgcattagtcaggcgaagctgcttttgagcactacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactctatttctcgcgagtatttaaaaaatgcaccggggccagcccgagcgagtttcgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0011_sequence/1
type: Sequence
persistent identity: BBa R0011 sequence
display id: BBa_R0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0422_sequence/1
type: Sequence
persistent identity: BBa E0422 sequence
display id: BBa_E0422_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0053_sequence/1
type: Sequence
persistent identity: BBa R0053 sequence
display id: BBa_R0053_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aataaacttgactaaagattcctttagtagataatttaagtgttctttaatttc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0080_sequence/1
type: Sequence
persistent identity: BBa R0080 sequence
display id: BBa_R0080_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I12202_sequence/1
type: Sequence
persistent identity: BBa I12202 sequence
display id: BBa_I12202_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I12203_sequence/1
type: Sequence
persistent identity: BBa I12203 sequence
display id: BBa_I12203_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagcgcagcaaacattgatgtggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I12205_sequence/1
type: Sequence
persistent identity: BBa I12205 sequence
display id: BBa_I12205_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggctgaagcgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggtttaacgccgattgaggccaacggttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctcaccattcgcggtcagggggtggtgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctgttcccgccaggagagattcatcactacggtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgcctactggcatgaatggcttaactggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagccgcatttcagcgacctgtttgggcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatctgcttgagcaattgttactgcggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgaggcttgtcagtacatcagcgatcacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcttgtcgccgtcgcgtctgtcacatcttttccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgcattagtcaggcgaagctgcttttgagcactacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactctatttctcgcgagtatttaaaaaatgcaccggggccagcccgagcgagtttcgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0022_sequence/1
type: Sequence
persistent identity: BBa E0022 sequence
display id: BBa_E0022_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0013_sequence/1
type: Sequence
persistent identity: BBa C0013 sequence
display id: BBa_C0013_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtaataacactgatagtgctagtgtagatcac
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z