BBa_I12233
type: ComponentDefinition
persistent identity: BBa I12233
display id: BBa_I12233
version: 1
was generated by: iGEM to SBOL conversion
description: TetR heterodimer test construct 2
created: 2004-08-31T11:00:00Z
modified: 2015-08-31T04:07:32Z
owned by: james
owned by: myers
mutable description: Same as the other TetR heterodimer test construct (I12232), but with different promoters in front of the two components of the heterodimer.
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 120
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: rwald
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_I0500
component: BBa_B0010
component: BBa_I12203
component: BBa_I12212
component: BBa_B0012
component: BBa_E0022
component: BBa_I12205
component: BBa_R0010
component: BBa_I12202
component: BBa_B0032
component: BBa_R0053
component: BBa_C0013
sequence annotation: BBa_R0053
sequence annotation: BBa_B0032
sequence annotation: BBa_C0013
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_I12205
sequence annotation: BBa_R0010
sequence annotation: BBa_I12202
sequence annotation: BBa_I0500
sequence annotation: BBa_B0034
sequence annotation: BBa_I12203
sequence annotation: BBa_I12212
sequence annotation: BBa_E0022
sequence: https://synbiohub.org/public/igem/BBa_I12233_sequence/1

BBa_B0034
type: Component
persistent identity: component1063113
display id: component1063113
version: 1
top level: BBa_I12233
definition: BBa_B0034
access: public

BBa_I0500
type: Component
persistent identity: component1063108
display id: component1063108
version: 1
top level: BBa_I12233
definition: pBad/araC
access: public

BBa_B0010
type: Component
persistent identity: component1063126
display id: component1063126
version: 1
top level: BBa_I12233
definition: BBa_B0010
access: public

BBa_I12203
type: Component
persistent identity: component1063120
display id: component1063120
version: 1
top level: BBa_I12233
definition: Tetr-4C
access: public

BBa_I12212
type: Component
persistent identity: component1063165
display id: component1063165
version: 1
top level: BBa_I12233
definition: TetR-4C
access: public

BBa_B0012
type: Component
persistent identity: component1063136
display id: component1063136
version: 1
top level: BBa_I12233
definition: BBa_B0012
access: public

BBa_E0022
type: Component
persistent identity: component1063182
display id: component1063182
version: 1
top level: BBa_I12233
definition: ECFP
access: public

BBa_B0034
type: Component
persistent identity: component1063170
display id: component1063170
version: 1
top level: BBa_I12233
definition: BBa_B0034
access: public

BBa_B0010
type: Component
persistent identity: component1063040
display id: component1063040
version: 1
top level: BBa_I12233
definition: BBa_B0010
access: public

BBa_I12205
type: Component
persistent identity: component1063034
display id: component1063034
version: 1
top level: BBa_I12233
definition: AraC
access: public

BBa_R0010
type: Component
persistent identity: component1063066
display id: component1063066
version: 1
top level: BBa_I12233
definition: LacI
access: public

BBa_B0012
type: Component
persistent identity: component1063050
display id: component1063050
version: 1
top level: BBa_I12233
definition: BBa_B0012
access: public

BBa_I12202
type: Component
persistent identity: component1063087
display id: component1063087
version: 1
top level: BBa_I12233
definition: TetR(B)
access: public

BBa_B0032
type: Component
persistent identity: component1063079
display id: component1063079
version: 1
top level: BBa_I12233
definition: BBa_B0032
access: public

BBa_B0012
type: Component
persistent identity: component1063103
display id: component1063103
version: 1
top level: BBa_I12233
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1063093
display id: component1063093
version: 1
top level: BBa_I12233
definition: BBa_B0010
access: public

BBa_R0053
type: Component
persistent identity: component1062960
display id: component1062960
version: 1
top level: BBa_I12233
definition: cII p22
access: public

BBa_C0013
type: Component
persistent identity: component1062978
display id: component1062978
version: 1
top level: BBa_I12233
definition: lacI
access: public

BBa_B0032
type: Component
persistent identity: component1062973
display id: component1062973
version: 1
top level: BBa_I12233
definition: BBa_B0032
access: public

BBa_B0012
type: Component
persistent identity: component1062994
display id: component1062994
version: 1
top level: BBa_I12233
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1062984
display id: component1062984
version: 1
top level: BBa_I12233
definition: BBa_B0010
access: public

BBa_B0032
type: Component
persistent identity: component1063026
display id: component1063026
version: 1
top level: BBa_I12233
definition: BBa_B0032
access: public

BBa_B0012
type: Component
persistent identity: component1063200
display id: component1063200
version: 1
top level: BBa_I12233
definition: BBa_B0012
access: public

BBa_R0053
type: Component
persistent identity: component1063013
display id: component1063013
version: 1
top level: BBa_I12233
definition: cII p22
access: public

BBa_B0010
type: Component
persistent identity: component1063190
display id: component1063190
version: 1
top level: BBa_I12233
definition: BBa_B0010
access: public

BBa_R0053
type: SequenceAnnotation
persistent identity: annotation1062960
display id: annotation1062960
version: 1
top level: BBa_I12233
location: https://synbiohub.org/public/igem/BBa_I12233/annotation1062960/range1062960/1
component: BBa_R0053

https://synbiohub.org/public/igem/BBa_I12233/annotation1062960/range1062960/1
type: Range
persistent identity: range1062960
display id: range1062960
version: 1
top level: BBa_I12233
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1062973
display id: annotation1062973
version: 1
top level: BBa_I12233
location: https://synbiohub.org/public/igem/BBa_I12233/annotation1062973/range1062973/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_I12233/annotation1062973/range1062973/1
type: Range
persistent identity: range1062973
display id: range1062973
version: 1
top level: BBa_I12233
direction: direction/forward
start: 63
end: 75
orientation: inline

BBa_C0013
type: SequenceAnnotation
persistent identity: annotation1062978
display id: annotation1062978
version: 1
top level: BBa_I12233
location: https://synbiohub.org/public/igem/BBa_I12233/annotation1062978/range1062978/1
component: BBa_C0013

https://synbiohub.org/public/igem/BBa_I12233/annotation1062978/range1062978/1
type: Range
persistent identity: range1062978
display id: range1062978
version: 1
top level: BBa_I12233
direction: direction/forward
start: 82
end: 1167
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1062984
display id: annotation1062984
version: 1
top level: BBa_I12233
location: https://synbiohub.org/public/igem/BBa_I12233/annotation1062984/range1062984/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I12233/annotation1062984/range1062984/1
type: Range
persistent identity: range1062984
display id: range1062984
version: 1
top level: BBa_I12233
direction: direction/forward
start: 1201
end: 1280
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1062994
display id: annotation1062994
version: 1
top level: BBa_I12233
location: https://synbiohub.org/public/igem/BBa_I12233/annotation1062994/range1062994/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I12233/annotation1062994/range1062994/1
type: Range
persistent identity: range1062994
display id: range1062994
version: 1
top level: BBa_I12233
direction: direction/forward
start: 1289
end: 1329
orientation: inline

BBa_R0053
type: SequenceAnnotation
persistent identity: annotation1063013
display id: annotation1063013
version: 1
top level: BBa_I12233
location: https://synbiohub.org/public/igem/BBa_I12233/annotation1063013/range1063013/1
component: BBa_R0053

https://synbiohub.org/public/igem/BBa_I12233/annotation1063013/range1063013/1
type: Range
persistent identity: range1063013
display id: range1063013
version: 1
top level: BBa_I12233
direction: direction/forward
start: 1338
end: 1391
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1063026
display id: annotation1063026
version: 1
top level: BBa_I12233
location: https://synbiohub.org/public/igem/BBa_I12233/annotation1063026/range1063026/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_I12233/annotation1063026/range1063026/1
type: Range
persistent identity: range1063026
display id: range1063026
version: 1
top level: BBa_I12233
direction: direction/forward
start: 1400
end: 1412
orientation: inline

BBa_I12205
type: SequenceAnnotation
persistent identity: annotation1063034
display id: annotation1063034
version: 1
top level: BBa_I12233
location: https://synbiohub.org/public/igem/BBa_I12233/annotation1063034/range1063034/1
component: BBa_I12205

https://synbiohub.org/public/igem/BBa_I12233/annotation1063034/range1063034/1
type: Range
persistent identity: range1063034
display id: range1063034
version: 1
top level: BBa_I12233
direction: direction/forward
start: 1419
end: 2300
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1063040
display id: annotation1063040
version: 1
top level: BBa_I12233
location: https://synbiohub.org/public/igem/BBa_I12233/annotation1063040/range1063040/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I12233/annotation1063040/range1063040/1
type: Range
persistent identity: range1063040
display id: range1063040
version: 1
top level: BBa_I12233
direction: direction/forward
start: 2334
end: 2413
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1063050
display id: annotation1063050
version: 1
top level: BBa_I12233
location: https://synbiohub.org/public/igem/BBa_I12233/annotation1063050/range1063050/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I12233/annotation1063050/range1063050/1
type: Range
persistent identity: range1063050
display id: range1063050
version: 1
top level: BBa_I12233
direction: direction/forward
start: 2422
end: 2462
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1063066
display id: annotation1063066
version: 1
top level: BBa_I12233
location: https://synbiohub.org/public/igem/BBa_I12233/annotation1063066/range1063066/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_I12233/annotation1063066/range1063066/1
type: Range
persistent identity: range1063066
display id: range1063066
version: 1
top level: BBa_I12233
direction: direction/forward
start: 2471
end: 2670
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1063079
display id: annotation1063079
version: 1
top level: BBa_I12233
location: https://synbiohub.org/public/igem/BBa_I12233/annotation1063079/range1063079/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_I12233/annotation1063079/range1063079/1
type: Range
persistent identity: range1063079
display id: range1063079
version: 1
top level: BBa_I12233
direction: direction/forward
start: 2679
end: 2691
orientation: inline

BBa_I12202
type: SequenceAnnotation
persistent identity: annotation1063087
display id: annotation1063087
version: 1
top level: BBa_I12233
location: https://synbiohub.org/public/igem/BBa_I12233/annotation1063087/range1063087/1
component: BBa_I12202

https://synbiohub.org/public/igem/BBa_I12233/annotation1063087/range1063087/1
type: Range
persistent identity: range1063087
display id: range1063087
version: 1
top level: BBa_I12233
direction: direction/forward
start: 2698
end: 3324
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1063093
display id: annotation1063093
version: 1
top level: BBa_I12233
location: https://synbiohub.org/public/igem/BBa_I12233/annotation1063093/range1063093/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I12233/annotation1063093/range1063093/1
type: Range
persistent identity: range1063093
display id: range1063093
version: 1
top level: BBa_I12233
direction: direction/forward
start: 3358
end: 3437
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1063103
display id: annotation1063103
version: 1
top level: BBa_I12233
location: https://synbiohub.org/public/igem/BBa_I12233/annotation1063103/range1063103/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I12233/annotation1063103/range1063103/1
type: Range
persistent identity: range1063103
display id: range1063103
version: 1
top level: BBa_I12233
direction: direction/forward
start: 3446
end: 3486
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation1063108
display id: annotation1063108
version: 1
top level: BBa_I12233
location: https://synbiohub.org/public/igem/BBa_I12233/annotation1063108/range1063108/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_I12233/annotation1063108/range1063108/1
type: Range
persistent identity: range1063108
display id: range1063108
version: 1
top level: BBa_I12233
direction: direction/forward
start: 3495
end: 4704
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1063113
display id: annotation1063113
version: 1
top level: BBa_I12233
location: https://synbiohub.org/public/igem/BBa_I12233/annotation1063113/range1063113/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I12233/annotation1063113/range1063113/1
type: Range
persistent identity: range1063113
display id: range1063113
version: 1
top level: BBa_I12233
direction: direction/forward
start: 4713
end: 4724
orientation: inline

BBa_I12203
type: SequenceAnnotation
persistent identity: annotation1063120
display id: annotation1063120
version: 1
top level: BBa_I12233
location: https://synbiohub.org/public/igem/BBa_I12233/annotation1063120/range1063120/1
component: BBa_I12203

https://synbiohub.org/public/igem/BBa_I12233/annotation1063120/range1063120/1
type: Range
persistent identity: range1063120
display id: range1063120
version: 1
top level: BBa_I12233
direction: direction/forward
start: 4731
end: 5357
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1063126
display id: annotation1063126
version: 1
top level: BBa_I12233
location: https://synbiohub.org/public/igem/BBa_I12233/annotation1063126/range1063126/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I12233/annotation1063126/range1063126/1
type: Range
persistent identity: range1063126
display id: range1063126
version: 1
top level: BBa_I12233
direction: direction/forward
start: 5391
end: 5470
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1063136
display id: annotation1063136
version: 1
top level: BBa_I12233
location: https://synbiohub.org/public/igem/BBa_I12233/annotation1063136/range1063136/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I12233/annotation1063136/range1063136/1
type: Range
persistent identity: range1063136
display id: range1063136
version: 1
top level: BBa_I12233
direction: direction/forward
start: 5479
end: 5519
orientation: inline

BBa_I12212
type: SequenceAnnotation
persistent identity: annotation1063165
display id: annotation1063165
version: 1
top level: BBa_I12233
location: https://synbiohub.org/public/igem/BBa_I12233/annotation1063165/range1063165/1
component: BBa_I12212

https://synbiohub.org/public/igem/BBa_I12233/annotation1063165/range1063165/1
type: Range
persistent identity: range1063165
display id: range1063165
version: 1
top level: BBa_I12233
direction: direction/forward
start: 5528
end: 5588
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1063170
display id: annotation1063170
version: 1
top level: BBa_I12233
location: https://synbiohub.org/public/igem/BBa_I12233/annotation1063170/range1063170/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I12233/annotation1063170/range1063170/1
type: Range
persistent identity: range1063170
display id: range1063170
version: 1
top level: BBa_I12233
direction: direction/forward
start: 5597
end: 5608
orientation: inline

BBa_E0022
type: SequenceAnnotation
persistent identity: annotation1063182
display id: annotation1063182
version: 1
top level: BBa_I12233
location: https://synbiohub.org/public/igem/BBa_I12233/annotation1063182/range1063182/1
component: BBa_E0022

https://synbiohub.org/public/igem/BBa_I12233/annotation1063182/range1063182/1
type: Range
persistent identity: range1063182
display id: range1063182
version: 1
top level: BBa_I12233
direction: direction/forward
start: 5615
end: 6376
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1063190
display id: annotation1063190
version: 1
top level: BBa_I12233
location: https://synbiohub.org/public/igem/BBa_I12233/annotation1063190/range1063190/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I12233/annotation1063190/range1063190/1
type: Range
persistent identity: range1063190
display id: range1063190
version: 1
top level: BBa_I12233
direction: direction/forward
start: 6385
end: 6464
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1063200
display id: annotation1063200
version: 1
top level: BBa_I12233
location: https://synbiohub.org/public/igem/BBa_I12233/annotation1063200/range1063200/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I12233/annotation1063200/range1063200/1
type: Range
persistent identity: range1063200
display id: range1063200
version: 1
top level: BBa_I12233
direction: direction/forward
start: 6473
end: 6513
orientation: inline

AraC
type: ComponentDefinition
persistent identity: BBa I12205
display id: BBa_I12205
version: 1
was generated by: iGEM to SBOL conversion
description: AraC
created: 2004-08-06T11:00:00Z
modified: 2015-08-31T04:07:31Z
owned by: james
owned by: myers
mutable description: The coding region for the AraC protein.
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 120
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: rwald
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: araC
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_I12205_sequence/1

araC
type: SequenceAnnotation
persistent identity: annotation1032104
display id: annotation1032104
version: 1
top level: AraC
location: https://synbiohub.org/public/igem/BBa_I12205/annotation1032104/range1032104/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I12205/annotation1032104/range1032104/1
type: Range
persistent identity: range1032104
display id: range1032104
version: 1
top level: AraC
direction: direction/forward
start: 1
end: 882
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214023
display id: annotation2214023
version: 1
top level: AraC
location: https://synbiohub.org/public/igem/BBa_I12205/annotation2214023/range2214023/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_I12205/annotation2214023/range2214023/1
type: Range
persistent identity: range2214023
display id: range2214023
version: 1
top level: AraC
direction: direction/forward
start: 883
end: 907
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

cII p22
type: ComponentDefinition
persistent identity: BBa R0053
display id: BBa_R0053
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (p22 cII regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Bacteriophage p22.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The p22 cII regulatory region sequence is a 97 base-pair sequence with the standard BioBrick prefix and suffix sections on its ends. p22 cII repressor protein, BBa_C0053, binds to it. This segment contains O-R1, O-R2, a fragment of O-R3, the -35 of P-RM, and P-R (-10 and -35 from Tom Knight)
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Maia Mahoney
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0053
sequence annotation: OR3
sequence annotation: -35
sequence annotation: OR2
sequence annotation: -10
sequence annotation: OR1
sequence: https://synbiohub.org/public/igem/BBa_R0053_sequence/1

BBa_R0053
type: SequenceAnnotation
persistent identity: annotation7069
display id: annotation7069
version: 1
top level: cII p22
location: https://synbiohub.org/public/igem/BBa_R0053/annotation7069/range7069/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0053/annotation7069/range7069/1
type: Range
persistent identity: range7069
display id: range7069
version: 1
top level: cII p22
direction: direction/forward
start: 1
end: 54
orientation: inline

OR3
type: SequenceAnnotation
persistent identity: annotation2035
display id: annotation2035
version: 1
top level: cII p22
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2035/range2035/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0053/annotation2035/range2035/1
type: Range
persistent identity: range2035
display id: range2035
version: 1
top level: cII p22
direction: direction/forward
start: 1
end: 3
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2041
display id: annotation2041
version: 1
top level: cII p22
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2041/range2041/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0053/annotation2041/range2041/1
type: Range
persistent identity: range2041
display id: range2041
version: 1
top level: cII p22
direction: direction/forward
start: 8
end: 13
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2036
display id: annotation2036
version: 1
top level: cII p22
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2036/range2036/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0053/annotation2036/range2036/1
type: Range
persistent identity: range2036
display id: range2036
version: 1
top level: cII p22
direction: direction/forward
start: 11
end: 28
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2038
display id: annotation2038
version: 1
top level: cII p22
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2038/range2038/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0053/annotation2038/range2038/1
type: Range
persistent identity: range2038
display id: range2038
version: 1
top level: cII p22
direction: direction/forward
start: 18
end: 23
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2042
display id: annotation2042
version: 1
top level: cII p22
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2042/range2042/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0053/annotation2042/range2042/1
type: Range
persistent identity: range2042
display id: range2042
version: 1
top level: cII p22
direction: direction/forward
start: 30
end: 35
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation2037
display id: annotation2037
version: 1
top level: cII p22
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2037/range2037/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0053/annotation2037/range2037/1
type: Range
persistent identity: range2037
display id: range2037
version: 1
top level: cII p22
direction: direction/forward
start: 34
end: 51
orientation: inline

TetR-4C
type: ComponentDefinition
persistent identity: BBa I12212
display id: BBa_I12212
version: 1
was generated by: iGEM to SBOL conversion
description: TetR - TetR-4C heterodimer promoter (negative)
created: 2004-08-05T11:00:00Z
modified: 2015-08-31T04:07:31Z
owned by: james
owned by: myers
mutable description: This promoter is repressed by a heterodimer of wild-type TetR and mutant TetR-4C. It differs from wild-type TetR promoter in that the +4 and -4 regions of its O2 binding site have been changed, and the -35 region of the reverse promoter (pA) has been destroyed.
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 120
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The O2 and O1 mutations are all single point mutations, occuring at the first base which appears listed here.
star: false
creator: rwald
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: -35
sequence annotation: TetR O2
sequence annotation: O2 mutation
sequence annotation: -10
sequence annotation: O1
sequence annotation: O1 mutation
sequence: https://synbiohub.org/public/igem/BBa_I12212_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation1031337
display id: annotation1031337
version: 1
top level: TetR-4C
location: https://synbiohub.org/public/igem/BBa_I12212/annotation1031337/range1031337/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_I12212/annotation1031337/range1031337/1
type: Range
persistent identity: range1031337
display id: range1031337
version: 1
top level: TetR-4C
direction: direction/forward
start: 1
end: 6
orientation: inline

TetR O2
type: SequenceAnnotation
persistent identity: annotation1031338
display id: annotation1031338
version: 1
top level: TetR-4C
location: https://synbiohub.org/public/igem/BBa_I12212/annotation1031338/range1031338/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_I12212/annotation1031338/range1031338/1
type: Range
persistent identity: range1031338
display id: range1031338
version: 1
top level: TetR-4C
direction: direction/forward
start: 2
end: 20
orientation: inline

O2 mutation
type: SequenceAnnotation
persistent identity: annotation1057573
display id: annotation1057573
version: 1
top level: TetR-4C
location: https://synbiohub.org/public/igem/BBa_I12212/annotation1057573/range1057573/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_I12212/annotation1057573/range1057573/1
type: Range
persistent identity: range1057573
display id: range1057573
version: 1
top level: TetR-4C
direction: direction/forward
start: 7
end: 7
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1031339
display id: annotation1031339
version: 1
top level: TetR-4C
location: https://synbiohub.org/public/igem/BBa_I12212/annotation1031339/range1031339/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I12212/annotation1031339/range1031339/1
type: Range
persistent identity: range1031339
display id: range1031339
version: 1
top level: TetR-4C
direction: direction/forward
start: 22
end: 27
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1031340
display id: annotation1031340
version: 1
top level: TetR-4C
location: https://synbiohub.org/public/igem/BBa_I12212/annotation1031340/range1031340/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I12212/annotation1031340/range1031340/1
type: Range
persistent identity: range1031340
display id: range1031340
version: 1
top level: TetR-4C
direction: direction/forward
start: 26
end: 30
orientation: inline

O1
type: SequenceAnnotation
persistent identity: annotation1031341
display id: annotation1031341
version: 1
top level: TetR-4C
location: https://synbiohub.org/public/igem/BBa_I12212/annotation1031341/range1031341/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_I12212/annotation1031341/range1031341/1
type: Range
persistent identity: range1031341
display id: range1031341
version: 1
top level: TetR-4C
direction: direction/forward
start: 32
end: 50
orientation: inline

O1 mutation
type: SequenceAnnotation
persistent identity: annotation1057574
display id: annotation1057574
version: 1
top level: TetR-4C
location: https://synbiohub.org/public/igem/BBa_I12212/annotation1057574/range1057574/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_I12212/annotation1057574/range1057574/1
type: Range
persistent identity: range1057574
display id: range1057574
version: 1
top level: TetR-4C
direction: direction/forward
start: 37
end: 37
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1031342
display id: annotation1031342
version: 1
top level: TetR-4C
location: https://synbiohub.org/public/igem/BBa_I12212/annotation1031342/range1031342/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I12212/annotation1031342/range1031342/1
type: Range
persistent identity: range1031342
display id: range1031342
version: 1
top level: TetR-4C
direction: direction/forward
start: 45
end: 50
orientation: inline

TetR(B)
type: ComponentDefinition
persistent identity: BBa I12202
display id: BBa_I12202
version: 1
was generated by: iGEM to SBOL conversion
description: Wild-type TetR(B) (-LVA)
created: 2004-08-05T11:00:00Z
modified: 2015-08-31T04:07:31Z
mutable provenance: Serratia marcescens
owned by: james
owned by: myers
mutable description: This is the standard wild-type TetR repressor. It may appear that this is a duplicate of BBa_C0041, but that part has an error near the end which breaks its stop codon. This was presumably to facilitate addition of an LVA tag; nonetheless, for the stand-alone protein, a stop codon is needed.
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 120
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: rwald
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: tetR
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_I12202_sequence/1

tetR
type: SequenceAnnotation
persistent identity: annotation1031374
display id: annotation1031374
version: 1
top level: TetR(B)
location: https://synbiohub.org/public/igem/BBa_I12202/annotation1031374/range1031374/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I12202/annotation1031374/range1031374/1
type: Range
persistent identity: range1031374
display id: range1031374
version: 1
top level: TetR(B)
direction: direction/forward
start: 1
end: 627
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214021
display id: annotation2214021
version: 1
top level: TetR(B)
location: https://synbiohub.org/public/igem/BBa_I12202/annotation2214021/range2214021/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_I12202/annotation2214021/range2214021/1
type: Range
persistent identity: range2214021
display id: range2214021
version: 1
top level: TetR(B)
direction: direction/forward
start: 628
end: 652
orientation: inline

Tetr-4C
type: ComponentDefinition
persistent identity: BBa I12203
display id: BBa_I12203
version: 1
was generated by: iGEM to SBOL conversion
description: TetR-4C
created: 2004-08-05T11:00:00Z
modified: 2015-08-31T04:07:31Z
owned by: james
owned by: myers
mutable description: This is a mutant form of TetR which binds to the tetO-4C operating region (rather than the standard tetO region). In particular, this is the EA37PQ39YM42 mutant.
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 120
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: rwald
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: tetR-4C
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_I12203_sequence/1

tetR-4C
type: SequenceAnnotation
persistent identity: annotation1031381
display id: annotation1031381
version: 1
top level: Tetr-4C
location: https://synbiohub.org/public/igem/BBa_I12203/annotation1031381/range1031381/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I12203/annotation1031381/range1031381/1
type: Range
persistent identity: range1031381
display id: range1031381
version: 1
top level: Tetr-4C
direction: direction/forward
start: 1
end: 627
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214022
display id: annotation2214022
version: 1
top level: Tetr-4C
location: https://synbiohub.org/public/igem/BBa_I12203/annotation2214022/range2214022/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_I12203/annotation2214022/range2214022/1
type: Range
persistent identity: range2214022
display id: range2214022
version: 1
top level: Tetr-4C
direction: direction/forward
start: 628
end: 652
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

pBad/araC
type: ComponentDefinition
persistent identity: BBa I0500
display id: BBa_I0500
version: 1
was generated by: iGEM to SBOL conversion
description: Inducible pBad/araC promoter
created: 2003-12-05T12:00:00Z
modified: 2015-08-31T04:07:29Z
owned by: james
owned by: myers
mutable description: -- No description --
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Sri Kosuri
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: AraC
sequence annotation: PC
sequence annotation: Pbad
sequence: https://synbiohub.org/public/igem/BBa_I0500_sequence/1

AraC
type: SequenceAnnotation
persistent identity: annotation1498843
display id: annotation1498843
version: 1
top level: pBad/araC
location: https://synbiohub.org/public/igem/BBa_I0500/annotation1498843/range1498843/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I0500/annotation1498843/range1498843/1
type: Range
persistent identity: range1498843
display id: range1498843
version: 1
top level: pBad/araC
direction: direction/forward
start: 1
end: 1079
orientation: inline

PC
type: SequenceAnnotation
persistent identity: annotation1498842
display id: annotation1498842
version: 1
top level: pBad/araC
location: https://synbiohub.org/public/igem/BBa_I0500/annotation1498842/range1498842/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I0500/annotation1498842/range1498842/1
type: Range
persistent identity: range1498842
display id: range1498842
version: 1
top level: pBad/araC
direction: direction/forward
start: 1043
end: 1114
orientation: inline

Pbad
type: SequenceAnnotation
persistent identity: annotation1498841
display id: annotation1498841
version: 1
top level: pBad/araC
location: https://synbiohub.org/public/igem/BBa_I0500/annotation1498841/range1498841/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_I0500/annotation1498841/range1498841/1
type: Range
persistent identity: range1498841
display id: range1498841
version: 1
top level: pBad/araC
direction: direction/forward
start: 1080
end: 1210
orientation: inline

ECFP
type: ComponentDefinition
persistent identity: BBa E0022
display id: BBa_E0022
version: 1
was generated by: iGEM to SBOL conversion
description: enhanced cyan fluorescent protein derived from A. victoria GFP
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:25Z
mutable provenance: Modified from <bb_part>BBa_E0021</bb_part>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Cyan fluorescent protein (ECFP) reporter coding sequence without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission.
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_E0022 cyan fluorescent protein is based on BioBrick part BBa_E0021. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000804
role: partType/Reporter
sequence annotation: BBa_E0022
sequence annotation: CFP (LVA)
sequence annotation: A
sequence annotation: SsrA
sequence annotation: 2
sequence: https://synbiohub.org/public/igem/BBa_E0022_sequence/1

BBa_E0022
type: SequenceAnnotation
persistent identity: annotation7040
display id: annotation7040
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation7040/range7040/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_E0022/annotation7040/range7040/1
type: Range
persistent identity: range7040
display id: range7040
version: 1
top level: ECFP
direction: direction/forward
start: 1
end: 762
orientation: inline

CFP (LVA)
type: SequenceAnnotation
persistent identity: annotation2150
display id: annotation2150
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2150/range2150/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E0022/annotation2150/range2150/1
type: Range
persistent identity: range2150
display id: range2150
version: 1
top level: ECFP
direction: direction/forward
start: 1
end: 762
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation2155
display id: annotation2155
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2155/range2155/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_E0022/annotation2155/range2155/1
type: Range
persistent identity: range2155
display id: range2155
version: 1
top level: ECFP
direction: direction/forward
start: 69
end: 69
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation2153
display id: annotation2153
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2153/range2153/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_E0022/annotation2153/range2153/1
type: Range
persistent identity: range2153
display id: range2153
version: 1
top level: ECFP
direction: direction/forward
start: 719
end: 756
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation2154
display id: annotation2154
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2154/range2154/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_E0022/annotation2154/range2154/1
type: Range
persistent identity: range2154
display id: range2154
version: 1
top level: ECFP
direction: direction/forward
start: 757
end: 762
orientation: inline

lacI
type: ComponentDefinition
persistent identity: BBa C0013
display id: BBa_C0013
version: 1
was generated by: iGEM to SBOL conversion
description: Repressor, lacI
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: www.ncbi.nlm.nih.gov
owned by: james
owned by: myers
mutable description: LacI repressor protein, without SsrA degradation tag. No Barcode.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Yong-Su Jin (Fighting Darwins)
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0013_sequence/1

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214005
display id: annotation2214005
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0013/annotation2214005/range2214005/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0013/annotation2214005/range2214005/1
type: Range
persistent identity: range2214005
display id: range2214005
version: 1
top level: lacI
direction: direction/forward
start: 1087
end: 1111
orientation: inline

https://synbiohub.org/public/igem/BBa_I12233_sequence/1
type: Sequence
persistent identity: BBa I12233 sequence
display id: BBa_I12233_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aataaacttgactaaagattcctttagtagataatttaagtgttctttaatttctactagagtcacacaggaaagtactagatgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaataaacttgactaaagattcctttagtagataatttaagtgttctttaatttctactagagtcacacaggaaagtactagatggctgaagcgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggtttaacgccgattgaggccaacggttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctcaccattcgcggtcagggggtggtgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctgttcccgccaggagagattcatcactacggtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgcctactggcatgaatggcttaactggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagccgcatttcagcgacctgtttgggcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatctgcttgagcaattgttactgcggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgaggcttgtcagtacatcagcgatcacctggcagacagcaat…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I12205_sequence/1
type: Sequence
persistent identity: BBa I12205 sequence
display id: BBa_I12205_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggctgaagcgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggtttaacgccgattgaggccaacggttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctcaccattcgcggtcagggggtggtgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctgttcccgccaggagagattcatcactacggtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgcctactggcatgaatggcttaactggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagccgcatttcagcgacctgtttgggcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatctgcttgagcaattgttactgcggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgaggcttgtcagtacatcagcgatcacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcttgtcgccgtcgcgtctgtcacatcttttccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgcattagtcaggcgaagctgcttttgagcactacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactctatttctcgcgagtatttaaaaaatgcaccggggccagcccgagcgagtttcgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I12212_sequence/1
type: Sequence
persistent identity: BBa I12212 sequence
display id: BBa_I12212_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttctctgtcactgatagggagtggtaaaataactctgtcaatgatagagtggattcaaaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I12202_sequence/1
type: Sequence
persistent identity: BBa I12202 sequence
display id: BBa_I12202_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I12203_sequence/1
type: Sequence
persistent identity: BBa I12203 sequence
display id: BBa_I12203_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagcgcagcaaacattgatgtggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0053_sequence/1
type: Sequence
persistent identity: BBa R0053 sequence
display id: BBa_R0053_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aataaacttgactaaagattcctttagtagataatttaagtgttctttaatttc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I0500_sequence/1
type: Sequence
persistent identity: BBa I0500 sequence
display id: BBa_I0500_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0022_sequence/1
type: Sequence
persistent identity: BBa E0022 sequence
display id: BBa_E0022_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0013_sequence/1
type: Sequence
persistent identity: BBa C0013 sequence
display id: BBa_C0013_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtaataacactgatagtgctagtgtagatcac
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z