LOCUS       BBa_I12240              2317 bp    DNA     linear       15-May-2024
DEFINITION  Test of LacI protein generator
ACCESSION   BBa_I12240
VERSION     BBa_I12240.1  
FEATURES             Location/Qualifiers
     misc_feature    1..3
                     /label=OR3
     misc_feature    11..28
                     /label=OR2
     misc_feature    34..51
                     /label=OR1
     promoter        18..23
                     /label=-35
     promoter        8..13
                     /label=-35
     promoter        30..35
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0053
     promoter        1..54
                     /label=BBa_R0053
     RBS             63..75
                     /label=RBS-3\Weak
     RBS             69..72
                     /label=RBS
     misc_feature    63..75
                     /label=BBa_B0032
     RBS             63..75
                     /label=BBa_B0032
     misc_feature    1168..1192
                     /label=Help:Barcodes
     CDS             82..1167
                     /label=BBa_C0013
     stem_loop       1212..1255
                     /label=stem_loop
     misc_feature    1201..1280
                     /label=BBa_B0010
     terminator      1201..1280
                     /label=BBa_B0010
     stem_loop       1296..1315
                     /label=T7 TE
     misc_feature    1322..1322
                     /label=stop
     polyA_site      1316..1329
                     /label=polya
     misc_feature    1289..1329
                     /label=BBa_B0012
     terminator      1289..1329
                     /label=BBa_B0012
     misc_feature    63..1329
                     /label=BBa_I12224
     misc_feature    1340..1356
                     /label=lac O1
     promoter        1357..1362
                     /label=-35
     misc_feature    1363..1379
                     /label=lac O1
     promoter        1380..1385
                     /label=-10
     misc_feature    1338..1391
                     /label=BBa_R0011
     promoter        1338..1391
                     /label=BBa_R0011
     misc_feature    1405..1408
                     /label=conserved
     RBS             1401..1412
                     /label=BBa_B0034
     CDS             1419..2180
                     /label=CFP (LVA)
     misc_feature    2137..2174
                     /label=SsrA
     misc_feature    2175..2180
                     /label=2
     misc_feature    1487..1487
                     /label=A
     misc_feature    1419..2180
                     /label=BBa_E0022
     misc_feature    1419..2180
                     /label=BBa_E0022
     stem_loop       2200..2243
                     /label=stem_loop
     misc_feature    2189..2268
                     /label=BBa_B0010
     terminator      2189..2268
                     /label=BBa_B0010
     stem_loop       2284..2303
                     /label=T7 TE
     misc_feature    2310..2310
                     /label=stop
     polyA_site      2304..2317
                     /label=polya
     misc_feature    2277..2317
                     /label=BBa_B0012
     terminator      2277..2317
                     /label=BBa_B0012
     misc_feature    1401..2317
                     /label=BBa_E0422
ORIGIN
        1 aataaacttg actaaagatt cctttagtag ataatttaag tgttctttaa tttctactag
       61 agtcacacag gaaagtacta gatgaaacca gtaacgttat acgatgtcgc agagtatgcc
      121 ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa
      181 acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt acattcccaa ccgcgtggca
      241 caacaactgg cgggcaaaca gtcgttgctg attggcgttg ccacctccag tctggccctg
      301 cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc
      361 gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat
      421 cttctcgcgc aacgcgtcag tgggctgatc attaactatc cgctggatga ccaggatgcc
      481 attgctgtgg aagctgcctg cactaatgtt ccggcgttat ttcttgatgt ctctgaccag
      541 acacccatca acagtattat tttctcccat gaagacggta cgcgactggg cgtggagcat
      601 ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg
      661 gcgcgtctgc gtctggctgg ctggcataaa tatctcactc gcaatcaaat tcagccgata
      721 gcggaacggg aaggcgactg gagtgccatg tccggttttc aacaaaccat gcaaatgctg
      781 aatgagggca tcgttcccac tgcgatgctg gttgccaacg atcagatggc gctgggcgca
      841 atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg atatctcggt agtgggatac
      901 gacgataccg aagacagctc atgttatatc ccgccgttaa ccaccatcaa acaggatttt
      961 cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg
     1021 aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat
     1081 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     1141 tcccgactgg aaagcgggca gtaataacac tgatagtgct agtgtagatc actactagag
     1201 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     1261 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
     1321 gcgtttatat actagagaat tgtgagcgga taacaattga cattgtgagc ggataacaag
     1381 atactgagca catactagag aaagaggaga aatactagat ggtgagcaag ggcgaggagc
     1441 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtgaac ggccacaagt
     1501 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
     1561 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctggg
     1621 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     1681 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
     1741 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     1801 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacatca
     1861 gccacaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc aacttcaaga
     1921 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
     1981 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
     2041 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
     2101 ccgggatcac tctcggcatg gacgagctgt acaagaggcc tgctgcaaac gacgaaaact
     2161 acgctttagt agcttaataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg
     2221 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac
     2281 actggctcac cttcgggtgg gcctttctgc gtttata
//