BBa_I12240
type: ComponentDefinition
persistent identity: BBa I12240
display id: BBa_I12240
version: 1
was generated by: iGEM to SBOL conversion
description: Test of LacI protein generator
created: 2004-08-31T11:00:00Z
modified: 2015-08-31T04:07:32Z
owned by: james
owned by: myers
mutable description: This part tests the effecacy of the I12224 protein generator.
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 120
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: rwald
type: DnaRegion
role: partType/Measurement
role: SO:0000804
component: BBa_I12224
component: BBa_R0053
component: BBa_E0422
component: BBa_R0011
sequence annotation: BBa_R0053
sequence annotation: BBa_I12224
sequence annotation: BBa_R0011
sequence annotation: BBa_E0422
sequence: https://synbiohub.org/public/igem/BBa_I12240_sequence/1

BBa_I12224
type: Component
persistent identity: component2229839
display id: component2229839
version: 1
top level: BBa_I12240
definition: BBa_I12224
access: public

BBa_R0053
type: Component
persistent identity: component2229822
display id: component2229822
version: 1
top level: BBa_I12240
definition: cII p22
access: public

BBa_E0422
type: Component
persistent identity: component2229858
display id: component2229858
version: 1
top level: BBa_I12240
definition: BBa_E0422
access: public

BBa_R0011
type: Component
persistent identity: component2229840
display id: component2229840
version: 1
top level: BBa_I12240
definition: lacI+pL
access: public

BBa_R0053
type: SequenceAnnotation
persistent identity: annotation2229822
display id: annotation2229822
version: 1
top level: BBa_I12240
location: https://synbiohub.org/public/igem/BBa_I12240/annotation2229822/range2229822/1
component: BBa_R0053

https://synbiohub.org/public/igem/BBa_I12240/annotation2229822/range2229822/1
type: Range
persistent identity: range2229822
display id: range2229822
version: 1
top level: BBa_I12240
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_I12224
type: SequenceAnnotation
persistent identity: annotation2229839
display id: annotation2229839
version: 1
top level: BBa_I12240
location: https://synbiohub.org/public/igem/BBa_I12240/annotation2229839/range2229839/1
component: BBa_I12224

https://synbiohub.org/public/igem/BBa_I12240/annotation2229839/range2229839/1
type: Range
persistent identity: range2229839
display id: range2229839
version: 1
top level: BBa_I12240
direction: direction/reverse
start: 63
end: 1329
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2229840
display id: annotation2229840
version: 1
top level: BBa_I12240
location: https://synbiohub.org/public/igem/BBa_I12240/annotation2229840/range2229840/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_I12240/annotation2229840/range2229840/1
type: Range
persistent identity: range2229840
display id: range2229840
version: 1
top level: BBa_I12240
direction: direction/forward
start: 1338
end: 1391
orientation: inline

BBa_E0422
type: SequenceAnnotation
persistent identity: annotation2229858
display id: annotation2229858
version: 1
top level: BBa_I12240
location: https://synbiohub.org/public/igem/BBa_I12240/annotation2229858/range2229858/1
component: BBa_E0422

https://synbiohub.org/public/igem/BBa_I12240/annotation2229858/range2229858/1
type: Range
persistent identity: range2229858
display id: range2229858
version: 1
top level: BBa_I12240
direction: direction/reverse
start: 1401
end: 2317
orientation: inline

BBa_I12224
type: ComponentDefinition
persistent identity: BBa I12224
display id: BBa_I12224
version: 1
was generated by: iGEM to SBOL conversion
description: LacI protein generator (LVA-)
created: 2004-08-06T11:00:00Z
modified: 2015-08-31T04:07:32Z
owned by: james
owned by: myers
mutable description: Protein generator for LacI (LVA-).
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 120
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: rwald
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_B0012
component: BBa_B0010
component: BBa_C0013
component: BBa_B0032
sequence annotation: BBa_B0032
sequence annotation: BBa_C0013
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I12224_sequence/1

BBa_B0012
type: Component
persistent identity: component1041957
display id: component1041957
version: 1
top level: BBa_I12224
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1041947
display id: component1041947
version: 1
top level: BBa_I12224
definition: BBa_B0010
access: public

BBa_C0013
type: Component
persistent identity: component1041941
display id: component1041941
version: 1
top level: BBa_I12224
definition: lacI
access: public

BBa_B0032
type: Component
persistent identity: component1041936
display id: component1041936
version: 1
top level: BBa_I12224
definition: BBa_B0032
access: public

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1041936
display id: annotation1041936
version: 1
top level: BBa_I12224
location: https://synbiohub.org/public/igem/BBa_I12224/annotation1041936/range1041936/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_I12224/annotation1041936/range1041936/1
type: Range
persistent identity: range1041936
display id: range1041936
version: 1
top level: BBa_I12224
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_C0013
type: SequenceAnnotation
persistent identity: annotation1041941
display id: annotation1041941
version: 1
top level: BBa_I12224
location: https://synbiohub.org/public/igem/BBa_I12224/annotation1041941/range1041941/1
component: BBa_C0013

https://synbiohub.org/public/igem/BBa_I12224/annotation1041941/range1041941/1
type: Range
persistent identity: range1041941
display id: range1041941
version: 1
top level: BBa_I12224
direction: direction/forward
start: 20
end: 1105
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1041947
display id: annotation1041947
version: 1
top level: BBa_I12224
location: https://synbiohub.org/public/igem/BBa_I12224/annotation1041947/range1041947/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I12224/annotation1041947/range1041947/1
type: Range
persistent identity: range1041947
display id: range1041947
version: 1
top level: BBa_I12224
direction: direction/forward
start: 1139
end: 1218
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1041957
display id: annotation1041957
version: 1
top level: BBa_I12224
location: https://synbiohub.org/public/igem/BBa_I12224/annotation1041957/range1041957/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I12224/annotation1041957/range1041957/1
type: Range
persistent identity: range1041957
display id: range1041957
version: 1
top level: BBa_I12224
direction: direction/forward
start: 1227
end: 1267
orientation: inline

lacI+pL
type: ComponentDefinition
persistent identity: BBa R0011
display id: BBa_R0011
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (lacI regulated, lambda pL hybrid)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site. LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
star: true
creator: Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0011
sequence annotation: lac O1
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0011_sequence/1

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation7064
display id: annotation7064
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
type: Range
persistent identity: range7064
display id: range7064
version: 1
top level: lacI+pL
direction: direction/forward
start: 1
end: 54
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation1999
display id: annotation1999
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
type: Range
persistent identity: range1999
display id: range1999
version: 1
top level: lacI+pL
direction: direction/forward
start: 3
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2000
display id: annotation2000
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
type: Range
persistent identity: range2000
display id: range2000
version: 1
top level: lacI+pL
direction: direction/forward
start: 20
end: 25
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation2001
display id: annotation2001
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
type: Range
persistent identity: range2001
display id: range2001
version: 1
top level: lacI+pL
direction: direction/forward
start: 26
end: 42
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2002
display id: annotation2002
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
type: Range
persistent identity: range2002
display id: range2002
version: 1
top level: lacI+pL
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_E0422
type: ComponentDefinition
persistent identity: BBa E0422
display id: BBa_E0422
version: 1
was generated by: iGEM to SBOL conversion
description: ECFP (RBS+ LVA+ TERM) (B0034.E0022.B0015)
created: 2003-12-04T12:00:00Z
modified: 2015-08-31T04:07:26Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: -- No description --
discontinued: false
dominant: true
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Caitlin Conboy and Jennifer Braff
type: DnaRegion
role: SO:0000804
role: partType/Reporter
component: BBa_B0034
component: BBa_B0010
component: BBa_E0022
component: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_E0022
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_E0422_sequence/1

BBa_B0034
type: Component
persistent identity: component942803
display id: component942803
version: 1
top level: BBa_E0422
definition: BBa_B0034
access: public

BBa_B0010
type: Component
persistent identity: component942823
display id: component942823
version: 1
top level: BBa_E0422
definition: BBa_B0010
access: public

BBa_E0022
type: Component
persistent identity: component942815
display id: component942815
version: 1
top level: BBa_E0422
definition: ECFP
access: public

BBa_B0012
type: Component
persistent identity: component942833
display id: component942833
version: 1
top level: BBa_E0422
definition: BBa_B0012
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation942803
display id: annotation942803
version: 1
top level: BBa_E0422
location: https://synbiohub.org/public/igem/BBa_E0422/annotation942803/range942803/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_E0422/annotation942803/range942803/1
type: Range
persistent identity: range942803
display id: range942803
version: 1
top level: BBa_E0422
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_E0022
type: SequenceAnnotation
persistent identity: annotation942815
display id: annotation942815
version: 1
top level: BBa_E0422
location: https://synbiohub.org/public/igem/BBa_E0422/annotation942815/range942815/1
component: BBa_E0022

https://synbiohub.org/public/igem/BBa_E0422/annotation942815/range942815/1
type: Range
persistent identity: range942815
display id: range942815
version: 1
top level: BBa_E0422
direction: direction/forward
start: 19
end: 780
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation942823
display id: annotation942823
version: 1
top level: BBa_E0422
location: https://synbiohub.org/public/igem/BBa_E0422/annotation942823/range942823/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_E0422/annotation942823/range942823/1
type: Range
persistent identity: range942823
display id: range942823
version: 1
top level: BBa_E0422
direction: direction/forward
start: 789
end: 868
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation942833
display id: annotation942833
version: 1
top level: BBa_E0422
location: https://synbiohub.org/public/igem/BBa_E0422/annotation942833/range942833/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_E0422/annotation942833/range942833/1
type: Range
persistent identity: range942833
display id: range942833
version: 1
top level: BBa_E0422
direction: direction/forward
start: 877
end: 917
orientation: inline

cII p22
type: ComponentDefinition
persistent identity: BBa R0053
display id: BBa_R0053
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (p22 cII regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Bacteriophage p22.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The p22 cII regulatory region sequence is a 97 base-pair sequence with the standard BioBrick prefix and suffix sections on its ends. p22 cII repressor protein, BBa_C0053, binds to it. This segment contains O-R1, O-R2, a fragment of O-R3, the -35 of P-RM, and P-R (-10 and -35 from Tom Knight)
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Maia Mahoney
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0053
sequence annotation: OR3
sequence annotation: -35
sequence annotation: OR2
sequence annotation: -10
sequence annotation: OR1
sequence: https://synbiohub.org/public/igem/BBa_R0053_sequence/1

BBa_R0053
type: SequenceAnnotation
persistent identity: annotation7069
display id: annotation7069
version: 1
top level: cII p22
location: https://synbiohub.org/public/igem/BBa_R0053/annotation7069/range7069/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0053/annotation7069/range7069/1
type: Range
persistent identity: range7069
display id: range7069
version: 1
top level: cII p22
direction: direction/forward
start: 1
end: 54
orientation: inline

OR3
type: SequenceAnnotation
persistent identity: annotation2035
display id: annotation2035
version: 1
top level: cII p22
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2035/range2035/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0053/annotation2035/range2035/1
type: Range
persistent identity: range2035
display id: range2035
version: 1
top level: cII p22
direction: direction/forward
start: 1
end: 3
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2041
display id: annotation2041
version: 1
top level: cII p22
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2041/range2041/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0053/annotation2041/range2041/1
type: Range
persistent identity: range2041
display id: range2041
version: 1
top level: cII p22
direction: direction/forward
start: 8
end: 13
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2036
display id: annotation2036
version: 1
top level: cII p22
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2036/range2036/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0053/annotation2036/range2036/1
type: Range
persistent identity: range2036
display id: range2036
version: 1
top level: cII p22
direction: direction/forward
start: 11
end: 28
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2038
display id: annotation2038
version: 1
top level: cII p22
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2038/range2038/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0053/annotation2038/range2038/1
type: Range
persistent identity: range2038
display id: range2038
version: 1
top level: cII p22
direction: direction/forward
start: 18
end: 23
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2042
display id: annotation2042
version: 1
top level: cII p22
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2042/range2042/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0053/annotation2042/range2042/1
type: Range
persistent identity: range2042
display id: range2042
version: 1
top level: cII p22
direction: direction/forward
start: 30
end: 35
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation2037
display id: annotation2037
version: 1
top level: cII p22
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2037/range2037/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0053/annotation2037/range2037/1
type: Range
persistent identity: range2037
display id: range2037
version: 1
top level: cII p22
direction: direction/forward
start: 34
end: 51
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

ECFP
type: ComponentDefinition
persistent identity: BBa E0022
display id: BBa_E0022
version: 1
was generated by: iGEM to SBOL conversion
description: enhanced cyan fluorescent protein derived from A. victoria GFP
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:25Z
mutable provenance: Modified from <bb_part>BBa_E0021</bb_part>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Cyan fluorescent protein (ECFP) reporter coding sequence without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission.
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_E0022 cyan fluorescent protein is based on BioBrick part BBa_E0021. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: BBa_E0022
sequence annotation: CFP (LVA)
sequence annotation: A
sequence annotation: SsrA
sequence annotation: 2
sequence: https://synbiohub.org/public/igem/BBa_E0022_sequence/1

BBa_E0022
type: SequenceAnnotation
persistent identity: annotation7040
display id: annotation7040
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation7040/range7040/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_E0022/annotation7040/range7040/1
type: Range
persistent identity: range7040
display id: range7040
version: 1
top level: ECFP
direction: direction/forward
start: 1
end: 762
orientation: inline

CFP (LVA)
type: SequenceAnnotation
persistent identity: annotation2150
display id: annotation2150
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2150/range2150/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E0022/annotation2150/range2150/1
type: Range
persistent identity: range2150
display id: range2150
version: 1
top level: ECFP
direction: direction/forward
start: 1
end: 762
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation2155
display id: annotation2155
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2155/range2155/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_E0022/annotation2155/range2155/1
type: Range
persistent identity: range2155
display id: range2155
version: 1
top level: ECFP
direction: direction/forward
start: 69
end: 69
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation2153
display id: annotation2153
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2153/range2153/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_E0022/annotation2153/range2153/1
type: Range
persistent identity: range2153
display id: range2153
version: 1
top level: ECFP
direction: direction/forward
start: 719
end: 756
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation2154
display id: annotation2154
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2154/range2154/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_E0022/annotation2154/range2154/1
type: Range
persistent identity: range2154
display id: range2154
version: 1
top level: ECFP
direction: direction/forward
start: 757
end: 762
orientation: inline

lacI
type: ComponentDefinition
persistent identity: BBa C0013
display id: BBa_C0013
version: 1
was generated by: iGEM to SBOL conversion
description: Repressor, lacI
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: www.ncbi.nlm.nih.gov
owned by: james
owned by: myers
mutable description: LacI repressor protein, without SsrA degradation tag. No Barcode.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Yong-Su Jin (Fighting Darwins)
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0013_sequence/1

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214005
display id: annotation2214005
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0013/annotation2214005/range2214005/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0013/annotation2214005/range2214005/1
type: Range
persistent identity: range2214005
display id: range2214005
version: 1
top level: lacI
direction: direction/forward
start: 1087
end: 1111
orientation: inline

https://synbiohub.org/public/igem/BBa_I12240_sequence/1
type: Sequence
persistent identity: BBa I12240 sequence
display id: BBa_I12240_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aataaacttgactaaagattcctttagtagataatttaagtgttctttaatttctactagagtcacacaggaaagtactagatgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I12224_sequence/1
type: Sequence
persistent identity: BBa I12224 sequence
display id: BBa_I12224_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaagtactagatgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0011_sequence/1
type: Sequence
persistent identity: BBa R0011 sequence
display id: BBa_R0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0422_sequence/1
type: Sequence
persistent identity: BBa E0422 sequence
display id: BBa_E0422_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0053_sequence/1
type: Sequence
persistent identity: BBa R0053 sequence
display id: BBa_R0053_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aataaacttgactaaagattcctttagtagataatttaagtgttctttaatttc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0022_sequence/1
type: Sequence
persistent identity: BBa E0022 sequence
display id: BBa_E0022_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0013_sequence/1
type: Sequence
persistent identity: BBa C0013 sequence
display id: BBa_C0013_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtaataacactgatagtgctagtgtagatcac
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z