LOCUS       BBa_I12241              1857 bp    DNA     linear       15-May-2024
DEFINITION  Test of TetR protein generator
ACCESSION   BBa_I12241
VERSION     BBa_I12241.1  
FEATURES             Location/Qualifiers
     misc_feature    1..3
                     /label=OR3
     misc_feature    11..28
                     /label=OR2
     misc_feature    34..51
                     /label=OR1
     promoter        18..23
                     /label=-35
     promoter        8..13
                     /label=-35
     promoter        30..35
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0053
     promoter        1..54
                     /label=BBa_R0053
     RBS             63..75
                     /label=RBS-3\Weak
     RBS             69..72
                     /label=RBS
     misc_feature    63..75
                     /label=BBa_B0032
     RBS             63..75
                     /label=BBa_B0032
     CDS             82..708
                     /label=tetR
     misc_feature    709..733
                     /label=Help:Barcodes
     CDS             82..708
                     /label=BBa_I12202
     stem_loop       753..796
                     /label=stem_loop
     misc_feature    742..821
                     /label=BBa_B0010
     terminator      742..821
                     /label=BBa_B0010
     stem_loop       837..856
                     /label=T7 TE
     misc_feature    863..863
                     /label=stop
     polyA_site      857..870
                     /label=polya
     misc_feature    830..870
                     /label=BBa_B0012
     terminator      830..870
                     /label=BBa_B0012
     misc_feature    879..897
                     /label=TetR 1
     misc_feature    879..932
                     /label=BBa_R0040
     promoter        898..903
                     /label=-35
     misc_feature    904..922
                     /label=TetR 2
     promoter        921..926
                     /label=-10
     promoter        879..932
                     /label=BBa_R0040
     misc_feature    945..948
                     /label=conserved
     RBS             941..952
                     /label=BBa_B0034
     CDS             959..1720
                     /label=CFP (LVA)
     misc_feature    1677..1714
                     /label=SsrA
     misc_feature    1715..1720
                     /label=2
     misc_feature    1027..1027
                     /label=A
     misc_feature    959..1720
                     /label=BBa_E0022
     misc_feature    959..1720
                     /label=BBa_E0022
     stem_loop       1740..1783
                     /label=stem_loop
     misc_feature    1729..1808
                     /label=BBa_B0010
     terminator      1729..1808
                     /label=BBa_B0010
     stem_loop       1824..1843
                     /label=T7 TE
     misc_feature    1850..1850
                     /label=stop
     polyA_site      1844..1857
                     /label=polya
     misc_feature    1817..1857
                     /label=BBa_B0012
     terminator      1817..1857
                     /label=BBa_B0012
ORIGIN
        1 aataaacttg actaaagatt cctttagtag ataatttaag tgttctttaa tttctactag
       61 agtcacacag gaaagtacta gatgtccaga ttagataaaa gtaaagtgat taacagcgca
      121 ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag
      181 ctaggtgtag agcagcctac attgtattgg catgtaaaaa ataagcgggc tttgctcgac
      241 gccttagcca ttgagatgtt agataggcac catactcact tttgcccttt agaaggggaa
      301 agctggcaag attttttacg taataacgct aaaagtttta gatgtgcttt actaagtcat
      361 cgcgatggag caaaagtaca tttaggtaca cggcctacag aaaaacagta tgaaactctc
      421 gaaaatcaat tagccttttt atgccaacaa ggtttttcac tagagaatgc attatatgca
      481 ctcagcgctg tggggcattt tactttaggt tgcgtattgg aagatcaaga gcatcaagtc
      541 gctaaagaag aaagggaaac acctactact gatagtatgc cgccattatt acgacaagct
      601 atcgaattat ttgatcacca aggtgcagag ccagccttct tattcggcct tgaattgatc
      661 atatgcggat tagaaaaaca acttaaatgt gaaagtgggt cttaataact ctgatagtgc
      721 tagtgtagat ctctactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact
      781 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
      841 caccttcggg tgggcctttc tgcgtttata tactagagtc cctatcagtg atagagattg
      901 acatccctat cagtgataga gatactgagc actactagag aaagaggaga aatactagat
      961 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     1021 cgacgtgaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
     1081 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     1141 cgtgaccacc ctgacctggg gcgtgcagtg cttcagccgc taccccgacc acatgaagca
     1201 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     1261 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     1321 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     1381 gctggagtac aactacatca gccacaacgt ctatatcacc gccgacaagc agaagaacgg
     1441 catcaaggcc aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
     1501 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     1561 cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     1621 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagaggcc
     1681 tgctgcaaac gacgaaaact acgctttagt agcttaataa tactagagcc aggcatcaaa
     1741 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
     1801 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//