##gff-version 3
##sequence-region BBa_I12241 1 1857
BBa_I12241	.	CDS	82	708	.	+	0	ID=BBa_I12202;Name=BBa_I12202
BBa_I12241	.	CDS	82	708	.	+	0	ID=annotation1031374;Name=tetR;Parent=BBa_I12202
BBa_I12241	.	engineered_tag	709	733	.	+	0	ID=annotation2214021;Name=Help:Barcodes;Parent=BBa_I12202
BBa_I12241	.	terminator	1729	1808	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I12241	.	stem_loop	1740	1783	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I12241	.	engineered_region	1729	1808	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I12241	.	terminator	1817	1857	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I12241	.	stem_loop	1824	1843	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I12241	.	stop_codon	1850	1850	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I12241	.	engineered_region	1817	1857	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I12241	.	polyA_site	1844	1857	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I12241	.	promoter	1	54	.	+	0	ID=BBa_R0053;Name=BBa_R0053
BBa_I12241	.	non_covalent_binding_site	34	51	.	+	0	ID=annotation2037;Name=OR1;Parent=BBa_R0053
BBa_I12241	.	promoter	8	13	.	+	0	ID=annotation2041;Name=-35;Parent=BBa_R0053
BBa_I12241	.	non_covalent_binding_site	1	3	.	+	0	ID=annotation2035;Name=OR3;Parent=BBa_R0053
BBa_I12241	.	engineered_region	1	54	.	+	0	ID=annotation7069;Name=BBa_R0053;Parent=BBa_R0053
BBa_I12241	.	promoter	30	35	.	+	0	ID=annotation2042;Name=-10;Parent=BBa_R0053
BBa_I12241	.	non_covalent_binding_site	11	28	.	+	0	ID=annotation2036;Name=OR2;Parent=BBa_R0053
BBa_I12241	.	promoter	18	23	.	+	0	ID=annotation2038;Name=-35;Parent=BBa_R0053
BBa_I12241	.	ribosome_entry_site	63	75	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_I12241	.	ribosome_entry_site	63	75	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_I12241	.	engineered_region	63	75	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_I12241	.	ribosome_entry_site	69	72	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_I12241	.	terminator	742	821	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I12241	.	stem_loop	753	796	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I12241	.	engineered_region	742	821	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I12241	.	ribosome_entry_site	941	952	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I12241	.	conserved_region	945	948	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I12241	.	terminator	830	870	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I12241	.	stem_loop	837	856	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I12241	.	stop_codon	863	863	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I12241	.	engineered_region	830	870	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I12241	.	polyA_site	857	870	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I12241	.	engineered_region	959	1720	.	+	0	ID=BBa_E0022;Name=BBa_E0022
BBa_I12241	.	CDS	959	1720	.	+	0	ID=annotation2150;Name=CFP (LVA);Parent=BBa_E0022
BBa_I12241	.	point_mutation	1027	1027	.	+	0	ID=annotation2155;Name=A;Parent=BBa_E0022
BBa_I12241	.	tag	1677	1714	.	+	0	ID=annotation2153;Name=SsrA;Parent=BBa_E0022
BBa_I12241	.	stop_codon	1715	1720	.	+	0	ID=annotation2154;Name=2;Parent=BBa_E0022
BBa_I12241	.	engineered_region	959	1720	.	+	0	ID=annotation7040;Name=BBa_E0022;Parent=BBa_E0022
BBa_I12241	.	promoter	879	932	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_I12241	.	engineered_region	879	932	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I12241	.	promoter	898	903	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I12241	.	non_covalent_binding_site	904	922	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I12241	.	promoter	921	926	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I12241	.	non_covalent_binding_site	879	897	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
>BBa_I12241
aataaacttgactaaagattcctttagtagataatttaagtgttctttaatttctactagagtcacacaggaaagtact
agatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaac
aacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctc
gacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttt
tacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcc
tacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcatta
tatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaa
gggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagcc
agccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtcttaataactc
tgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcg
ttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttat
atactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagagga
gaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtg
aacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgca
ccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccc
cgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaag
gacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggca
tcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgc
cgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgac
cactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccc
tgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcat
ggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatca
aataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagt
cacactggctcaccttcgggtgggcctttctgcgtttata