LOCUS       BBa_I12243              2207 bp    DNA     linear       14-May-2024
DEFINITION  Test of AraC protein generator
ACCESSION   BBa_I12243
VERSION     BBa_I12243.1  
FEATURES             Location/Qualifiers
     misc_feature    1..3
                     /label=OR3
     misc_feature    11..28
                     /label=OR2
     misc_feature    34..51
                     /label=OR1
     promoter        18..23
                     /label=-35
     promoter        8..13
                     /label=-35
     promoter        30..35
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0053
     promoter        1..54
                     /label=BBa_R0053
     RBS             63..75
                     /label=RBS-3\Weak
     RBS             69..72
                     /label=RBS
     misc_feature    63..75
                     /label=BBa_B0032
     RBS             63..75
                     /label=BBa_B0032
     CDS             82..963
                     /label=araC
     misc_feature    964..988
                     /label=Help:Barcodes
     CDS             82..963
                     /label=BBa_I12205
     stem_loop       1008..1051
                     /label=stem_loop
     misc_feature    997..1076
                     /label=BBa_B0010
     terminator      997..1076
                     /label=BBa_B0010
     stem_loop       1092..1111
                     /label=T7 TE
     misc_feature    1118..1118
                     /label=stop
     polyA_site      1112..1125
                     /label=polya
     misc_feature    1085..1125
                     /label=BBa_B0012
     terminator      1085..1125
                     /label=BBa_B0012
     misc_feature    1137..1162
                     /label=c-amp2
     misc_feature    1139..1177
                     /label=araO1
     misc_feature    1176..1205
                     /label=c-amp1
     misc_feature    1206..1234
                     /label=ara1 and ara2
     promoter        1246..1251
                     /label=-35
     promoter        1269..1274
                     /label=-10
     promoter        1134..1282
                     /label=BBa_R0080
     misc_feature    1295..1298
                     /label=conserved
     RBS             1291..1302
                     /label=BBa_B0034
     CDS             1309..2070
                     /label=CFP (LVA)
     misc_feature    2027..2064
                     /label=SsrA
     misc_feature    2065..2070
                     /label=2
     misc_feature    1377..1377
                     /label=A
     misc_feature    1309..2070
                     /label=BBa_E0022
     misc_feature    1309..2070
                     /label=BBa_E0022
     stem_loop       2090..2133
                     /label=stem_loop
     misc_feature    2079..2158
                     /label=BBa_B0010
     terminator      2079..2158
                     /label=BBa_B0010
     stem_loop       2174..2193
                     /label=T7 TE
     misc_feature    2200..2200
                     /label=stop
     polyA_site      2194..2207
                     /label=polya
     misc_feature    2167..2207
                     /label=BBa_B0012
     terminator      2167..2207
                     /label=BBa_B0012
ORIGIN
        1 aataaacttg actaaagatt cctttagtag ataatttaag tgttctttaa tttctactag
       61 agtcacacag gaaagtacta gatggctgaa gcgcaaaatg atcccctgct gccgggatac
      121 tcgtttaacg cccatctggt ggcgggttta acgccgattg aggccaacgg ttatctcgat
      181 ttttttatcg accgaccgct gggaatgaaa ggttatattc tcaatctcac cattcgcggt
      241 cagggggtgg tgaaaaatca gggacgagaa tttgtctgcc gaccgggtga tattttgctg
      301 ttcccgccag gagagattca tcactacggt cgtcatccgg aggctcgcga atggtatcac
      361 cagtgggttt actttcgtcc gcgcgcctac tggcatgaat ggcttaactg gccgtcaata
      421 tttgccaata cgggtttctt tcgcccggat gaagcgcacc agccgcattt cagcgacctg
      481 tttgggcaaa tcattaacgc cgggcaaggg gaagggcgct attcggagct gctggcgata
      541 aatctgcttg agcaattgtt actgcggcgc atggaagcga ttaacgagtc gctccatcca
      601 ccgatggata atcgggtacg cgaggcttgt cagtacatca gcgatcacct ggcagacagc
      661 aattttgata tcgccagcgt cgcacagcat gtttgcttgt cgccgtcgcg tctgtcacat
      721 cttttccgcc agcagttagg gattagcgtc ttaagctggc gcgaggacca acgcattagt
      781 caggcgaagc tgcttttgag cactacccgg atgcctatcg ccaccgtcgg tcgcaatgtt
      841 ggttttgacg atcaactcta tttctcgcga gtatttaaaa aatgcaccgg ggccagcccg
      901 agcgagtttc gtgccggttg tgaagaaaaa gtgaatgatg tagccgtcaa gttgtcataa
      961 taacgctgat agtgctagtg tagatcgcta ctagagccag gcatcaaata aaacgaaagg
     1021 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
     1081 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta gaggcgtaac
     1141 aaaagtgtct ataatcacgg cagaaaagtc cacattgatt atttgcacgg cgtcacactt
     1201 tgctatgcca tagcattttt atccataaga ttagcggatc ctacctgacg ctttttatcg
     1261 caactctcta ctgtttctcc attactagag aaagaggaga aatactagat ggtgagcaag
     1321 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtgaac
     1381 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     1441 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     1501 ctgacctggg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     1561 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     1621 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     1681 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     1741 aactacatca gccacaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc
     1801 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     1861 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     1921 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     1981 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagaggcc tgctgcaaac
     2041 gacgaaaact acgctttagt agcttaataa tactagagcc aggcatcaaa taaaacgaaa
     2101 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta
     2161 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//