##gff-version 3
##sequence-region BBa_I12243 1 2207
BBa_I12243	.	promoter	1	54	.	+	0	ID=BBa_R0053;Name=BBa_R0053
BBa_I12243	.	non_covalent_binding_site	1	3	.	+	0	ID=annotation2035;Name=OR3;Parent=BBa_R0053
BBa_I12243	.	promoter	18	23	.	+	0	ID=annotation2038;Name=-35;Parent=BBa_R0053
BBa_I12243	.	engineered_region	1	54	.	+	0	ID=annotation7069;Name=BBa_R0053;Parent=BBa_R0053
BBa_I12243	.	promoter	30	35	.	+	0	ID=annotation2042;Name=-10;Parent=BBa_R0053
BBa_I12243	.	non_covalent_binding_site	11	28	.	+	0	ID=annotation2036;Name=OR2;Parent=BBa_R0053
BBa_I12243	.	non_covalent_binding_site	34	51	.	+	0	ID=annotation2037;Name=OR1;Parent=BBa_R0053
BBa_I12243	.	promoter	8	13	.	+	0	ID=annotation2041;Name=-35;Parent=BBa_R0053
BBa_I12243	.	ribosome_entry_site	63	75	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_I12243	.	ribosome_entry_site	63	75	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_I12243	.	ribosome_entry_site	69	72	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_I12243	.	engineered_region	63	75	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_I12243	.	promoter	1134	1282	.	+	0	ID=BBa_R0080;Name=BBa_R0080
BBa_I12243	.	non_covalent_binding_site	1176	1205	.	+	0	ID=annotation301458;Name=c-amp1;Parent=BBa_R0080
BBa_I12243	.	non_covalent_binding_site	1137	1162	.	+	0	ID=annotation301456;Name=c-amp2;Parent=BBa_R0080
BBa_I12243	.	non_covalent_binding_site	1206	1234	.	+	0	ID=annotation301462;Name=ara1 and ara2;Parent=BBa_R0080
BBa_I12243	.	promoter	1269	1274	.	+	0	ID=annotation308602;Name=-10;Parent=BBa_R0080
BBa_I12243	.	promoter	1246	1251	.	+	0	ID=annotation308601;Name=-35;Parent=BBa_R0080
BBa_I12243	.	operator	1139	1177	.	+	0	ID=annotation301457;Name=araO1;Parent=BBa_R0080
BBa_I12243	.	terminator	2079	2158	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I12243	.	engineered_region	2079	2158	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I12243	.	stem_loop	2090	2133	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I12243	.	CDS	82	963	.	+	0	ID=BBa_I12205;Name=BBa_I12205
BBa_I12243	.	engineered_tag	964	988	.	+	0	ID=annotation2214023;Name=Help:Barcodes;Parent=BBa_I12205
BBa_I12243	.	CDS	82	963	.	+	0	ID=annotation1032104;Name=araC;Parent=BBa_I12205
BBa_I12243	.	terminator	2167	2207	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I12243	.	polyA_site	2194	2207	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I12243	.	engineered_region	2167	2207	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I12243	.	stem_loop	2174	2193	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I12243	.	stop_codon	2200	2200	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I12243	.	terminator	997	1076	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I12243	.	engineered_region	997	1076	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I12243	.	stem_loop	1008	1051	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I12243	.	ribosome_entry_site	1291	1302	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I12243	.	conserved_region	1295	1298	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I12243	.	engineered_region	1309	2070	.	+	0	ID=BBa_E0022;Name=BBa_E0022
BBa_I12243	.	CDS	1309	2070	.	+	0	ID=annotation2150;Name=CFP (LVA);Parent=BBa_E0022
BBa_I12243	.	point_mutation	1377	1377	.	+	0	ID=annotation2155;Name=A;Parent=BBa_E0022
BBa_I12243	.	tag	2027	2064	.	+	0	ID=annotation2153;Name=SsrA;Parent=BBa_E0022
BBa_I12243	.	stop_codon	2065	2070	.	+	0	ID=annotation2154;Name=2;Parent=BBa_E0022
BBa_I12243	.	engineered_region	1309	2070	.	+	0	ID=annotation7040;Name=BBa_E0022;Parent=BBa_E0022
BBa_I12243	.	terminator	1085	1125	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I12243	.	polyA_site	1112	1125	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I12243	.	engineered_region	1085	1125	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I12243	.	stem_loop	1092	1111	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I12243	.	stop_codon	1118	1118	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
>BBa_I12243
aataaacttgactaaagattcctttagtagataatttaagtgttctttaatttctactagagtcacacaggaaagtact
agatggctgaagcgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggtttaacgccgat
tgaggccaacggttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctcaccattcgc
ggtcagggggtggtgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctgttcccgccaggagaga
ttcatcactacggtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgcctactggca
tgaatggcttaactggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagccgcatttcagc
gacctgtttgggcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatctgcttgagc
aattgttactgcggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgaggcttgtca
gtacatcagcgatcacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcttgtcgccgtcgcgt
ctgtcacatcttttccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgcattagtcaggcgaagc
tgcttttgagcactacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactctatttctcgcg
agtatttaaaaaatgcaccggggccagcccgagcgagtttcgtgccggttgtgaagaaaaagtgaatgatgtagccgtc
aagttgtcataataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtc
gaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggt
gggcctttctgcgtttatatactagaggcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttg
cacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaac
tctctactgtttctccattactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggt
ggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgcc
acctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccc
tgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccga
aggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggc
gacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagt
acaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgcca
caacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctg
cccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctgg
agttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgc
tttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttat
ctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata