LOCUS BBa_I12244 3268 bp DNA linear 22-Nov-2024 DEFINITION Test of AraC protein generator 2 ACCESSION BBa_I12244 VERSION BBa_I12244.1 FEATURES Location/Qualifiers misc_feature 1..3 /label=OR3 misc_feature 11..28 /label=OR2 misc_feature 34..51 /label=OR1 promoter 18..23 /label=-35 promoter 8..13 /label=-35 promoter 30..35 /label=-10 misc_feature 1..54 /label=BBa_R0053 promoter 1..54 /label=BBa_R0053 RBS 63..75 /label=RBS-3\Weak RBS 69..72 /label=RBS misc_feature 63..75 /label=BBa_B0032 RBS 63..75 /label=BBa_B0032 CDS 82..963 /label=araC misc_feature 964..988 /label=Help:Barcodes CDS 82..963 /label=BBa_I12205 stem_loop 1008..1051 /label=stem_loop misc_feature 997..1076 /label=BBa_B0010 terminator 997..1076 /label=BBa_B0010 stem_loop 1092..1111 /label=T7 TE misc_feature 1118..1118 /label=stop polyA_site 1112..1125 /label=polya misc_feature 1085..1125 /label=BBa_B0012 terminator 1085..1125 /label=BBa_B0012 promoter 2213..2343 /label=Pbad promoter 2176..2247 /label=PC misc_feature 1134..2212 /label=AraC promoter 1134..2343 /label=BBa_I0500 misc_feature 2356..2359 /label=conserved RBS 2352..2363 /label=BBa_B0034 CDS 2370..3131 /label=CFP (LVA) misc_feature 3088..3125 /label=SsrA misc_feature 3126..3131 /label=2 misc_feature 2438..2438 /label=A misc_feature 2370..3131 /label=BBa_E0022 misc_feature 2370..3131 /label=BBa_E0022 stem_loop 3151..3194 /label=stem_loop misc_feature 3140..3219 /label=BBa_B0010 terminator 3140..3219 /label=BBa_B0010 stem_loop 3235..3254 /label=T7 TE misc_feature 3261..3261 /label=stop polyA_site 3255..3268 /label=polya misc_feature 3228..3268 /label=BBa_B0012 terminator 3228..3268 /label=BBa_B0012 ORIGIN 1 aataaacttg actaaagatt cctttagtag ataatttaag tgttctttaa tttctactag 61 agtcacacag gaaagtacta gatggctgaa gcgcaaaatg atcccctgct gccgggatac 121 tcgtttaacg cccatctggt ggcgggttta acgccgattg aggccaacgg ttatctcgat 181 ttttttatcg accgaccgct gggaatgaaa ggttatattc tcaatctcac cattcgcggt 241 cagggggtgg tgaaaaatca gggacgagaa tttgtctgcc gaccgggtga tattttgctg 301 ttcccgccag gagagattca tcactacggt cgtcatccgg aggctcgcga atggtatcac 361 cagtgggttt actttcgtcc gcgcgcctac tggcatgaat ggcttaactg gccgtcaata 421 tttgccaata cgggtttctt tcgcccggat gaagcgcacc agccgcattt cagcgacctg 481 tttgggcaaa tcattaacgc cgggcaaggg gaagggcgct attcggagct gctggcgata 541 aatctgcttg agcaattgtt actgcggcgc atggaagcga ttaacgagtc gctccatcca 601 ccgatggata atcgggtacg cgaggcttgt cagtacatca gcgatcacct ggcagacagc 661 aattttgata tcgccagcgt cgcacagcat gtttgcttgt cgccgtcgcg tctgtcacat 721 cttttccgcc agcagttagg gattagcgtc ttaagctggc gcgaggacca acgcattagt 781 caggcgaagc tgcttttgag cactacccgg atgcctatcg ccaccgtcgg tcgcaatgtt 841 ggttttgacg atcaactcta tttctcgcga gtatttaaaa aatgcaccgg ggccagcccg 901 agcgagtttc gtgccggttg tgaagaaaaa gtgaatgatg tagccgtcaa gttgtcataa 961 taacgctgat agtgctagtg tagatcgcta ctagagccag gcatcaaata aaacgaaagg 1021 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact 1081 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagttatgac 1141 aacttgacgg ctacatcatt cactttttct tcacaaccgg cacggaactc gctcgggctg 1201 gccccggtgc attttttaaa tacccgcgag aaatagagtt gatcgtcaaa accaacattg 1261 cgaccgacgg tggcgatagg catccgggtg gtgctcaaaa gcagcttcgc ctggctgata 1321 cgttggtcct cgcgccagct taagacgcta atccctaact gctggcggaa aagatgtgac 1381 agacgcgacg gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa attgctgtct 1441 gccaggtgat cgctgatgta ctgacaagcc tcgcgtaccc gattatccat cggtggatgg 1501 agcgactcgt taatcgcttc catgcgccgc agtaacaatt gctcaagcag atttatcgcc 1561 agcagctccg aatagcgccc ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg 1621 ctgaaatgcg gctggtgcgc ttcatccggg cgaaagaacc ccgtattggc aaatattgac 1681 ggccagttaa gccattcatg ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac 1741 cattcgcgag cctccggatg acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa 1801 atatcacccg gtcggcaaac aaattctcgt ccctgatttt tcaccacccc ctgaccgcga 1861 atggtgagat tgagaatata acctttcatt cccagcggtc ggtcgataaa aaaatcgaga 1921 taaccgttgg cctcaatcgg cgttaaaccc gccaccagat gggcattaaa cgagtatccc 1981 ggcagcaggg gatcattttg cgcttcagcc atacttttca tactcccgcc attcagagaa 2041 gaaaccaatt gtccatattg catcagacat tgccgtcact gcgtctttta ctggctcttc 2101 tcgctaacca aaccggtaac cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa 2161 gccatgacaa aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt ccacattgat 2221 tatttgcacg gcgtcacact ttgctatgcc atagcatttt tatccataag attagcggat 2281 cctacctgac gctttttatc gcaactctct actgtttctc catacccgtt tttttgggct 2341 agctactaga gaaagaggag aaatactaga tggtgagcaa gggcgaggag ctgttcaccg 2401 gggtggtgcc catcctggtc gagctggacg gcgacgtgaa cggccacaag ttcagcgtgt 2461 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca 2521 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctgg ggcgtgcagt 2581 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg 2641 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg 2701 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact 2761 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacatc agccacaacg 2821 tctatatcac cgccgacaag cagaagaacg gcatcaaggc caacttcaag atccgccaca 2881 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg 2941 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag 3001 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca 3061 ctctcggcat ggacgagctg tacaagaggc ctgctgcaaa cgacgaaaac tacgctttag 3121 tagcttaata atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg 3181 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca 3241 ccttcgggtg ggcctttctg cgtttata //