LOCUS BBa_I13012 942 bp DNA linear 22-Dec-2024 DEFINITION RBS Test R0040.B0031.E0130 ACCESSION BBa_I13012 VERSION BBa_I13012.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 69..72 /label=conserved RBS 63..76 /label=BBa_B0031 CDS 83..805 /label=BBa_E0030 stem_loop 825..868 /label=stem_loop misc_feature 814..893 /label=BBa_B0010 terminator 814..893 /label=BBa_B0010 stem_loop 909..928 /label=T7 TE misc_feature 935..935 /label=stop polyA_site 929..942 /label=polya misc_feature 902..942 /label=BBa_B0012 terminator 902..942 /label=BBa_B0012 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agtcacacag gaaacctact agatggtgag caagggcgag gagctgttca ccggggtggt 121 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga 181 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa 241 gctgcccgtg ccctggccca ccctcgtgac caccttcggc tacggcctgc aatgcttcgc 301 ccgctacccc gaccacatga agctgcacga cttcttcaag tccgccatgc ccgaaggcta 361 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt 421 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga 481 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat 541 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga 601 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc 661 cgtgctgctg cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa 721 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg 781 catggacgag ctgtacaagt aataatacta gagccaggca tcaaataaaa cgaaaggctc 841 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga 901 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta //