LOCUS       BBa_I13020              1815 bp    DNA     linear       15-May-2024
DEFINITION  QPI Test Construct Intermediate (Q04530.E0430)
ACCESSION   BBa_I13020
VERSION     BBa_I13020.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..666
                     /label=cII p22
     misc_feature    667..699
                     /label=LVA
     misc_feature    700..705
                     /label=stop
     misc_feature    19..705
                     /label=BBa_C0053
     misc_feature    706..730
                     /label=Help:Barcodes
     CDS             19..705
                     /label=BBa_C0053
     stem_loop       750..793
                     /label=stem_loop
     misc_feature    739..818
                     /label=BBa_B0010
     terminator      739..818
                     /label=BBa_B0010
     stem_loop       834..853
                     /label=T7 TE
     misc_feature    860..860
                     /label=stop
     polyA_site      854..867
                     /label=polya
     misc_feature    827..867
                     /label=BBa_B0012
     terminator      827..867
                     /label=BBa_B0012
     misc_feature    876..878
                     /label=OR3
     misc_feature    886..903
                     /label=OR2
     misc_feature    909..926
                     /label=OR1
     promoter        893..898
                     /label=-35
     promoter        883..888
                     /label=-35
     promoter        905..910
                     /label=-10
     misc_feature    876..929
                     /label=BBa_R0053
     promoter        876..929
                     /label=BBa_R0053
     misc_feature    942..945
                     /label=conserved
     RBS             938..949
                     /label=BBa_B0034
     CDS             956..1678
                     /label=BBa_E0030
     stem_loop       1698..1741
                     /label=stem_loop
     misc_feature    1687..1766
                     /label=BBa_B0010
     terminator      1687..1766
                     /label=BBa_B0010
     stem_loop       1782..1801
                     /label=T7 TE
     misc_feature    1808..1808
                     /label=stop
     polyA_site      1802..1815
                     /label=polya
     misc_feature    1775..1815
                     /label=BBa_B0012
     terminator      1775..1815
                     /label=BBa_B0012
ORIGIN
        1 aaagaggaga aatactagat gaatacacaa ttgatgggtg agcgtattcg cgctcgaaga
       61 aaaaaactca agattagaca agccgctctt ggtaagatgg tgggagtgtc taatgttgca
      121 atatcgcaat gggagcgctc ggagactgag ccaaatgggg agaacctgtt ggcactttcg
      181 aaggctcttc agtgctcccc tgactatttg ctgaaaggag atttaagcca gacaaacgtt
      241 gcctatcata gtaggcatga gccaagagga tcataccctc ttatcagttg ggtaagcgca
      301 gggcaatgga tggaagctgt agaaccttat cacaagcgcg cgatagagaa ctggcacgac
      361 accactgtag attgttcaga agattcattt tggcttgatg tccaaggtga ctctatgaca
      421 gcaccggcag ggttaagcat tccagaagga atgataattc tggttgatcc cgaagtcgaa
      481 ccaagaaacg gcaagctggt tgttgcaaaa ttagaaggtg aaaacgaggc cacattcaaa
      541 aaattagtta tggatgcagg ccgaaagttt ttaaaaccat taaacccaca atatccgatg
      601 atagaaatca acggaaactg caaaatcatt ggcgtagttg ttgacgcaaa actcgcaaat
      661 cttccagctg caaacgacga aaactacgct ttagtagctt aataacactg atagtgctag
      721 tgtagatcac tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
      781 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac
      841 cttcgggtgg gcctttctgc gtttatatac tagagaataa acttgactaa agattccttt
      901 agtagataat ttaagtgttc tttaatttct actagagaaa gaggagaaat actagatggt
      961 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     1021 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     1081 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     1141 gaccaccttc ggctacggcc tgcaatgctt cgcccgctac cccgaccaca tgaagctgca
     1201 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     1261 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     1321 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     1381 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
     1441 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
     1501 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     1561 gagctaccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     1621 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtaataata
     1681 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     1741 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
     1801 ctttctgcgt ttata
//