LOCUS BBa_I13021 2257 bp DNA linear 22-Dec-2024 DEFINITION QPI Test Construct Intermediate (Q04121.E0430) ACCESSION BBa_I13021 VERSION BBa_I13021.1 FEATURES Location/Qualifiers misc_feature 5..8 /label=conserved RBS 1..12 /label=BBa_B0034 misc_feature 1108..1146 /label=LVA CDS 19..1146 /label=lacI-LVA misc_feature 19..1146 /label=BBa_C0012 misc_feature 1147..1171 /label=Help:Barcodes CDS 19..1146 /label=BBa_C0012 stem_loop 1191..1234 /label=stem_loop misc_feature 1180..1259 /label=BBa_B0010 terminator 1180..1259 /label=BBa_B0010 stem_loop 1275..1294 /label=T7 TE misc_feature 1301..1301 /label=stop polyA_site 1295..1308 /label=polya misc_feature 1268..1308 /label=BBa_B0012 terminator 1268..1308 /label=BBa_B0012 terminator 1180..1308 /label=BBa_B0015 misc_feature 1319..1335 /label=lac O1 promoter 1336..1341 /label=-35 misc_feature 1342..1358 /label=lac O1 promoter 1359..1364 /label=-10 misc_feature 1317..1370 /label=BBa_R0011 promoter 1317..1370 /label=BBa_R0011 misc_feature 1..1371 /label=BBa_Q04121 misc_feature 1384..1387 /label=conserved RBS 1380..1391 /label=BBa_B0034 CDS 1398..2120 /label=BBa_E0030 stem_loop 2140..2183 /label=stem_loop misc_feature 2129..2208 /label=BBa_B0010 terminator 2129..2208 /label=BBa_B0010 stem_loop 2224..2243 /label=T7 TE misc_feature 2250..2250 /label=stop polyA_site 2244..2257 /label=polya misc_feature 2217..2257 /label=BBa_B0012 terminator 2217..2257 /label=BBa_B0012 misc_feature 1380..2257 /label=BBa_E0430 ORIGIN 1 aaagaggaga aatactagat ggtgaatgtg aaaccagtaa cgttatacga tgtcgcagag 61 tatgccggtg tctcttatca gaccgtttcc cgcgtggtga accaggccag ccacgtttct 121 gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat tcccaaccgc 181 gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac ctccagtctg 241 gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt 301 gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg 361 cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta actatccgct ggatgaccag 421 gatgccattg ctgtggaagc tgcctgcact aatgttccgg cgttatttct tgatgtctct 481 gaccagacac ccatcaacag tattattttc tcccatgaag acggtacgcg actgggcgtg 541 gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc attaagttct 601 gtctcggcgc gtctgcgtct ggctggctgg cataaatatc tcactcgcaa tcaaattcag 661 ccgatagcgg aacgggaagg cgactggagt gccatgtccg gttttcaaca aaccatgcaa 721 atgctgaatg agggcatcgt tcccactgcg atgctggttg ccaacgatca gatggcgctg 781 ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg 841 ggatacgacg ataccgaaga cagctcatgt tatatcccgc cgttaaccac catcaaacag 901 gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag 961 gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccctggcg 1021 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga 1081 caggtttccc gactggaaag cgggcaggct gcaaacgacg aaaactacgc tttagtagct 1141 taataactct gatagtgcta gtgtagatct ctactagagc caggcatcaa ataaaacgaa 1201 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct 1261 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagaatt 1321 gtgagcggat aacaattgac attgtgagcg gataacaaga tactgagcac atactagaga 1381 aagaggagaa atactagatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca 1441 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg 1501 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc 1561 ccgtgccctg gcccaccctc gtgaccacct tcggctacgg cctgcaatgc ttcgcccgct 1621 accccgacca catgaagctg cacgacttct tcaagtccgc catgcccgaa ggctacgtcc 1681 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt 1741 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg 1801 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg 1861 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg 1921 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc 1981 tgctgcccga caaccactac ctgagctacc agtccgccct gagcaaagac cccaacgaga 2041 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg 2101 acgagctgta caagtaataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg 2161 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac 2221 actggctcac cttcgggtgg gcctttctgc gtttata //